Method for stakeholder identification in interorganizational environments

Stakeholders are the first emerging challenge in any software project. Their identification is a critical task for success. Nevertheless, many authors consider them as a default product of a non-explained identification process. Several aspects must be considered when the project is carried out in environments where multiple organizations interact. These complex contexts demand extremely hard efforts. Stakeholders must be identified taking into account their attributes (types, roles), which must be extended and defined for these environments. In general, there are no methodologies that allow performing this task in a systematic way for the development of interorganizational information systems. The paper proposes a method for carrying out stakeholder identification considering the diverse dimensions involved in interorganizational environments: organizational, interorganizational and external. It allows the systematic specification of all the people, groups and organizations whose interests and needs can affect or are affected by the interorganizational system. Also diverse stakeholders’ attributes such as types, roles, influence and interest are defined, analyzed, and included in the method. They are all important in later stages of any software project.     

Monogenic Autoinflammatory Diseases: State of the Art and Future Perspectives

Systemic autoinflammatory diseases are a heterogeneous family of disorders characterized by a dysregulation of the innate immune system, in which sterile inflammation primarily develops through antigen-independent hyperactivation of immune pathways. In most cases, they have a strong genetic background, with mutations in single genes involved in inflammation. Therefore, they can derive from different pathogenic mechanisms at any level, such as dysregulated inflammasome-mediated production of cytokines, intracellular stress, defective regulatory pathways, altered protein folding, enhanced NF-kappaB signalling, ubiquitination disorders, interferon pathway upregulation and complement activation. Since the discover of pathogenic mutations of the pyrin-encoding gene MEFV in Familial Mediterranean Fever, more than 50 monogenic autoinflammatory diseases have been discovered thanks to the advances in genetic sequencing: the advent of new genetic analysis techniques and the discovery of genes involved in autoinflammatory diseases have allowed a better understanding of the underlying innate immunologic pathways and pathogenetic mechanisms, thus opening new perspectives in targeted therapies. Moreover, this field of research has become of great interest, since more than a hundred clinical trials for autoinflammatory diseases are currently active or recently concluded, allowing us to hope for considerable acquisitions for the next few years. General paediatricians need to be aware of the importance of this group of diseases and they should consider autoinflammatory diseases in patients with clinical hallmarks, in order to guide further examinations and refer the patient to a specialist rheumatologist. Here we resume the pathogenesis, clinical aspects and diagnosis of the most important autoinflammatory diseases in children.     

Life cycle assessment of a multi-megawatt wind turbine

At the present moment in time, renewable energy sources have achieved great significance for modern day society. The main reason for this boom is the need to use alternative sources of energy to fossil fuels which are free of CO₂ emissions and contamination. Among the current renewable energy sources, the growth of wind farms has been spectacular. Wind power uses the kinetic energy of the wind to produce a clean form of energy without producing contamination or emissions. The problem it raises is that of quantifying to what extent it is a totally clean form of energy. In this sense we have to consider not only the emissions produced while they are in operation, but also the contamination and environmental impact resulting from their manufacture and the future dismantling of the turbines when they come to the end of their working life. The aim of this study is to analyse the real impact that this technology has if we consider the whole life cycle. The application of the ISO 14040 standard [ISO. ISO 14040. Environmental management – life cycle assessment – principles and framework. Geneva, Switzerland: International Standard Organization; 1998.] allows us to make an LCA study quantifying the overall impact of a wind turbine and each of its components.Applying this methodology, the wind turbine is analysed during all the phases of its life cycle, from cradle to grave, with regard to the manufacture of its key components (through the incorporation of cut-off criteria), transport to the wind farm, subsequent installation, start-up, maintenance and final dismantling and stripping down into waste materials and their treatment.     

On mobility-fluctuation origin of noise

The bases and implications of models ascribing 1/ƒ noise to the fluctuations in the number and mobility of the carriers are reviewed and compared, their symmetry and equivalence are shown and new results are proposed. It is proved that local and uncorrelated fluctuations in the mobility need variations in the total density of the carriers, which, however, do not affect the short-circuit current, and that they are due and equivalent to the fluctuations in the defect charges. These and other results are reached by determining in a general way the coupling equation between the local and the output fluctuations, and by finding through it that the short-circuit current fluctuations are given simply by the integral over the sample of the mobility variations or, in an equivalent way, of the fluctuations in the defect charges. The mobility variance for the various models is also computed and it is shown that, for some of them, it reaches values too high to be easily explained.     

Effects of milk high pressure homogenization on biogenic amine accumulation during ripening of ovine and bovine Italian cheeses

The aim of this work was to evaluate the biogenic amine (BA) content during the ripening of both bovine and ovine cheeses obtained using milk subjected to a homogenization treatment at 100 MPa before cheese-making. The data obtained were compared with those from cheeses produced by the same milks without any treatment or thermized. The results showed that both microbial ecology and BA concentrations of cheeses during ripening were significantly influenced by the type of milk used for cheese-making and by the treatment applied to the raw materials. In particular, the microbial counts found in Caciotta indicated that the high pressure homogenization (HPH) of milk significantly reduced the presence of the yeasts, Micrococcaceae and lactobacilli at the end of ripening. On the other hand, the HPH treatment of milk favoured the proliferation of yeasts in ovine cheese. Moreover, the ovine cheeses were characterized by a remarkably higher accumulation of BA than bovine cheeses. However, the HPH treatment of milk was able to drastically reduce the biogenic amine concentrations in both cheese typologies at the end of ripening.     

Disruption of syntaxin-mediated protein interactions blocks neurotransmitter secretion

The membrane protein syntaxin participates in several protein-protein interactions that have been implicated in neurotransmitter release. To probe the physiological importance of these interactions, we microinjected into the squid giant presynaptic terminal botulinum toxin C1, which cleaves syntaxin, and the H3 domain of syntaxin, which mediates binding to other proteins. Both reagents inhibited synaptic transmission yet did not affect the number or distribution of synaptic vesicles at the presynaptic active zone. Recombinant H3 domain inhibited the interactions between syntaxin and SNAP-25 that underlie the formation of stable SNARE complexes in vitro. These data support the notion that syntaxin-mediated SNARE complexes are necessary for docked synaptic vesicles to fuse.     

Classroom contextual effects on children's play.

Examined the effects of age, sex, and context of preschool classrooms on children's play. In Study 1, observed 3-, 4-, and 5-year-olds playing in art, blocks, and replica play areas in their age-graded classrooms. Results suggested that children's play was related to Age?×?Play Area interactions. These relations were not independent of children's sex-related self-selection into the play areas or the number of participants in the areas. In Study 2, same-age, same-sex dyads played with blocks and replica props in experimental playrooms. Play was mediated by age, sex, and play area. Girls' play was less sophisticated than boys' play in the blocks area because it was an area in which males tended to play. Furthermore, the ecological validity of Study 2 was established vis-à-vis Study 1 results. In Study 3, same-age, mixed-sex dyads played in constructive and replica areas in experimental playrooms. No sex or Sex?×?Context effects were observed on play. Boys tended to dominate girls in both contexts. Results are explained in terms of Lewin's model of context. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation

The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271⁺ and CD271⁻ mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271⁺ and CD271⁻ mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271⁺, CD271⁻ mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271⁺ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271⁺ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.