Kamil Wierzchowski  Bartosz Nowak  Mateusz Kawka  Patryk Wi&#x;ckowicz  Katarzyna D&#x;bkowska-Susfa&#x;  Agnieszka Pietrosiuk  Katarzyna Syk&#x;owska-Baranek  Maciej Pilarek 《International journal of molecular sciences》2022,23(22)

In situ extraction is a method for separating plant secondary metabolites from in vitro systems of plant biomass cultures. The study aimed to investigate the MTMS-based xerogels morphology effect on the growth kinetics and deoxyshikonin productivity in xerogel-supported in vitro culture systems of Rindera graeca hairy root. Cultures were supplemented with three types of xerogel, i.e., mesoporous gel, microporous gel, and agglomerated precipitate, in the disintegrated or monolithic form. Structure, oil sorption capacity, and SEM analyses for xerogel-based additives were performed. Application of monolithic macroporous xerogel resulted in the highest biomass proliferation, i.e., 5.11-fold fresh biomass increase after four weeks of the screening culture. The highest deoxyshikonin production (i.e., 105.03 µg) was noted when hairy roots were maintained with particles of disintegrated mesoporous xerogel. The detailed kinetics investigations (6-week culture) revealed the highest growth of hairy root biomass and secondary metabolite production, equaling 9.46-fold fresh weight biomass and 204.08 µg deoxyshikonin, respectively. MTMS-based xerogels have been recognized as selective biocompatible scaffolds for boosting the proliferation of transgenic roots or for productivity enhancement of naphthoquinones without detrimental effects on biomass growth, and their successful applicability in in situ removal of secondary plant metabolites has been experimentally confirmed.  相似文献   

    

Annika-Ricarda Kuhn  Marc van Bilsen 《International journal of molecular sciences》2022,23(22)

Heart failure is associated with profound alterations in cardiac intermediary metabolism. One of the prevailing hypotheses is that metabolic remodeling leads to a mismatch between cardiac energy (ATP) production and demand, thereby impairing cardiac function. However, even after decades of research, the relevance of metabolic remodeling in the pathogenesis of heart failure has remained elusive. Here we propose that cardiac metabolic remodeling should be looked upon from more perspectives than the mere production of ATP needed for cardiac contraction and relaxation. Recently, advances in cancer research have revealed that the metabolic rewiring of cancer cells, often coined as oncometabolism, directly impacts cellular phenotype and function. Accordingly, it is well feasible that the rewiring of cardiac cellular metabolism during the development of heart failure serves similar functions. In this review, we reflect on the influence of principal metabolic pathways on cellular phenotype as originally described in cancer cells and discuss their potential relevance for cardiac pathogenesis. We discuss current knowledge of metabolism-driven phenotypical alterations in the different cell types of the heart and evaluate their impact on cardiac pathogenesis and therapy.  相似文献   

    

Sabine Heublein  Udo Jeschke  Cornelia Sattler  Christina Kuhn  Anna Hester  Bastian Czogalla  Fabian Trillsch  Sven Mahner  Doris Mayr  Elisa Schmoeckel  Nina Ditsch 《International journal of molecular sciences》2022,23(5)

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Jaroslaw Markowski  Marcel Zambrzycki  Wojciech Smolka  Agnieszka Panek  Maciej Gubernat  Pawe&#x; Czaja  Mateusz Marzec  Aneta Fraczek-Szczypta 《International journal of molecular sciences》2022,23(11)

The main aim of this study is to investigate the effect of fragmentation of electrospun carbon nanofibers (eCNFs) obtained at different temperatures, i.e., at 750 °C, 1000 °C, 1500 °C, 1750 °C and 2000 °C on the cellular response in vitro. In order to assess the influence of nanofibers on biological response, it was necessary to conduct physicochemical, microstructural and structural studies such as SEM, XPS, Raman spectroscopy, HRTEM and surface wettability of the obtained materials. During the in vitro study, all samples made contact with the human chondrocyte CHON-001 cell lines. The key study was to assess the genotoxicity of eCNFs using the comet test after 1 h or 24 h. Special attention was paid to the degree of crystallinity of the nanofibers, the dimensions of the degradation products and the presence of functional groups on their surface. A detailed analysis showed that the key determinant of the genotoxic effect is the surface chemistry. The presence of nitrogen-containing groups as a product of the decomposition of nitrile groups has an influence on the biological response, leading to mutations in the DNA. This effect was observed only for samples carbonized at lower temperatures, i.e., 750 °C and 1000 °C. These results are important with respect to selecting the temperature of thermal treatment of eCNFs dedicated for medical and environmental functions due to the minimization of the genotoxic effect of these materials.  相似文献   

    

Nicolau López-Pintó  Christopher J. Jensen  Zhijie Chen  Zhengwei Tan  Zheng Ma  Maciej Oskar Liedke  Maik Butterling  Andreas Wagner  Javier Herrero-Martín  Enric Menéndez  Josep Nogués  Kai Liu  Jordi Sort 《Advanced functional materials》2024,34(42):2404487

The increasing energy demand in information technologies requires novel low-power procedures to store and process data. Magnetic materials, central to these technologies, are usually controlled through magnetic fields or spin-polarized currents that are prone to the Joule heating effect. Magneto-ionics is a unique energy-efficient strategy to control magnetism that can induce large non-volatile modulation of magnetization, coercivity and other properties through voltage-driven ionic motion. Recent studies have shown promising magneto-ionic effects using nitrogen ions. However, either liquid electrolytes or prior annealing procedures are necessary to induce the desired N-ion motion. In this work, magneto-ionic effects are voltage-triggered at room temperature in solid state systems of Co_xMn_1-xN films, without the need of thermal annealing. Upon gating, a rearrangement of nitrogen ions in the layers is observed, leading to changes in the co-existing ferromagnetic and antiferromagnetic phases, which result in substantial increase of magnetization at room temperature and modulation of the exchange bias effect at low temperatures. A detailed correlation between the structural and magnetic evolution of the system upon voltage actuation is provided. The obtained results offer promising new avenues for the utilization of nitride compounds in energy-efficient spintronic and other memory devices.  相似文献   

    

Eva Miglbauer  Dr. Oliya S. Abdullaeva  Dr. Maciej Gryszel  Dr. Eric Daniel Głowacki 《Chembiochem : a European journal of chemical biology》2023,24(17):e202300353

Reactive oxygen species (ROS) are an integral part of many anticancer therapies. Fenton-like processes involving reactions of peroxides with transition metal ions are a particularly potent and tunable subset of ROS approaches. Precise on-demand dosing of the Fenton reaction is an area of great interest. Herein, we present a concept of an electrochemical faradaic pixel that produces controlled amounts of ROS via a Fenton-like process. The pixel comprises a cathode and anode, where the cathode reduces dissolved oxygen to hydrogen peroxide. The anode is made of chromium, which is electrochemically corroded to yield chromium ions. Peroxide and chromium interact to form a highly oxidizing mixture of hydroxyl radicals and hexavalent Cr ions. After benchmarking the electrochemical properties of this type of device, we demonstrate how it can be used under in vitro conditions with a cancer cell line. The faradaic Fenton pixel is a general and scalable concept that can be used for on-demand delivery of redox-active products for controlling a physiological outcome.  相似文献   

    

Georg Linz  Sebastian Bernhard Rauer  Yasmin Kuhn  Simon Wennemaring  Laura Siedler  Smriti Singh  Matthias Wessling 《Advanced Materials Technologies》2021,6(6):2100009

Cell culture experiments often suffer from limited commercial availability of laboratory-scale bioreactors, which allow experiments to be conducted under flow conditions and additional online monitoring techniques. A novel 3D-printed bioreactor with a homogeneously distributed flow field enabling epithelial cell culture experiments and online barrier monitoring by integrated electrodes through electrical impedance spectroscopy (EIS) is presented. Transparent and conductive indium tin oxide glass as current-injecting electrodes allows direct visualization of the cells, while measuring EIS simultaneously. The bioreactor's design considers the importance of a homogeneous electric field by placing the voltage pick-up electrodes in the electrical field. The device's functionality is demonstrated by the cultivation of the epithelial cell line Caco-2 under continuous flow and monitoring of the cell layer by online EIS. The collected EIS data were fitted by an equivalent electric circuit, resulting in the cell layer's resistance and capacitance. This data is used to monitor the cell layer's reaction to ethylene glycol-bis-(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid and forskolin. These two model substances show the power of impedance spectroscopy as a non-invasive way to characterize cell barriers. In addition, the bioreactor design is available as a print-ready file in the Appendix, enabling its use for other scientific institutions.  相似文献   

    

John Banhart  Zi Yang  Qianning Guo  Meng Liu  Maik Butterling  Maciej Oskar Liedke  Eric Hirschmann  Andreas Wagner 《Advanced Engineering Materials》2024,26(4):2300490

The binding between vacancies and Mg atoms in an aluminum solid solution is not fully understood but essential for understanding its role in age hardening of many Al alloys. After annealing and quenching, Mg prevents the loss of excess vacancies during natural ageing and forms complexes containing one, possibly two, vacancies, and various Mg atoms. By heating the alloy after natural ageing, these complexes are dissolved, i.e., natural ageing is reverted. This reversion process is studied by in situ positron annihilation lifetime spectroscopy utilizing the very high count rate at the accelerator driven facility ELBE. Positron spectra are continuously acquired during heating at rates between 3 and 50 K min⁻¹. After correcting for the contributions of the oxidized surface and decomposing spectra into components, the process can be followed in detail and is found to take place in distinct stages: first, the number of vacancy–Mg complexes is reduced and then the liberated vacancies agglomerate into clusters that eventually dissolve at even higher temperatures.  相似文献   

    

Maciej A. Noras  Misa N. Vo 《国际能源研究杂志》2020,44(4):2998-3007

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Fernanda Kuhn  Eduarda Silva de Azevedo  Caciano Pelayo Zapata Noreña 《Journal of Food Processing and Preservation》2020,44(10):e14834

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