Multifunctional carbon-nanotube cellular endoscopes

Glass micropipettes, atomic force microscope tips and nanoneedles can be used to interrogate cells, but these devices either have conical geometries that can damage cells during penetration or are incapable of continuous fluid handling. Here, we report a carbon-nanotube-based endoscope for interrogating cells, transporting fluids and performing optical and electrochemical diagnostics at the single organelle level. The endoscope, which is made by placing a multiwalled carbon nanotube (length, 50-60?μm) at the tip of a glass pipette, can probe the intracellular environment with a spatial resolution of ～100?nm and can also access organelles without disrupting the cell. When the nanotube is filled with magnetic nanoparticles, the endoscope can be remotely manoeuvered to transport nanoparticles and attolitre volumes of fluids to and from precise locations. Because they are mounted on conventional glass micropipettes, the endoscopes readily fit standard instruments, creating a broad range of opportunities for minimally invasive intracellular probing, drug delivery and single-cell surgery.     

4种马铃薯淀粉糊流变特性的研究

对4种不同加工方式获得的马铃薯淀粉糊流变特性进行了探讨。静态流变特性测定结果表明,4种淀粉糊的流变模型为幂率模型,均为假塑性流体,每种淀粉糊以相同方式流动需要施加的力不同。在相同流动方式下,淀粉4粘度最大,其次为淀粉2,淀粉1和淀粉3的粘度近似相同。动态流变特性测定结果表明:随着温度的增加,淀粉1、2、4的弹性模量、粘性模量刚开始基本不发生变化,到60℃后,两模量急剧增加到最大值,然后降低到某恒定值。淀粉3的两模量随温度升高逐渐增加到最大值,然后逐渐降低到某恒定值。粘性模量达到最大后,淀粉2的粘性模量最大,其次为淀粉4、淀粉1,淀粉3的最低。弹性模量达到最大后,淀粉4的弹性模量最大,其次为淀粉2、淀粉1,淀粉3最低。     

Using Planar Embedded DRAM in Memory Intensive Signal Processing Circuits: Case Studies on LDPC Decoding and Motion Estimation

This paper studies the feasibility and potential of using planar embedded DRAM (eDRAM), which is completely compatible with CMOS logic process, to improve circuit implementation efficiency of memory-hungry signal processing algorithms. In spite of its apparent cell area efficiency advantage over SRAM, planar eDRAM is not being widely used in practice, mainly due to its very short retention time (e.g., few $\upmu $ s and even a few hundreds ns). In this work, we contend that short retention time may not necessarily be a fundamental issue for implementing signal processing algorithms because they typically handle streaming data, which exhibits regular and predictable data access patterns, and has a large algorithm/architecture design space. This study elaborates on the rationale and application of using a planar eDRAM in memory-hungry signal processing circuit implementations, and discusses the possible algorithm and architecture design strategies to better embrace the use of planar eDRAM. For the purpose of demonstration, we use low-density parity-check (LDPC) code decoding and motion estimation in video encoding as test vehicles. Beyond a straightforward SRAM replacement, we propose an interleaved read/write page-mode DRAM operation to reduce planar eDRAM energy consumption by leveraging LDPC code decoding data access pattern, and we investigate the potential of using planar eDRAM to enable a higher degree of image data reuse in motion estimation by proposing a folded scan structure to further improve its effectiveness. We carried out detailed planar eDRAM SPICE simulations at 45 nm node to obtain its characteristics, based on which we quantitatively evaluate the effectiveness of using planar eDRAM in these two case studies.     

Mathematical modeling and verification of pulse electrochemical micromachining of microtools

Pulse electrochemical micromachining (PECMM) is an unconventional manufacturing method suitable for the production of micro-sized components on a wide range of electrically conductive materials. PECMM in this study has been used to manufacture microtools. The non-contact nature of PECMM has necessitated the modeling of the process to estimate the anodic profile (microtool profile). This paper presents a mathematical model for predicting the diameter of the microtools fabricated by PECMM process. Tungsten microtools of diameters less than 100 μm were fabricated using an in-house built microelectrochemical machining system. Experimental results confirm the theoretical prediction of reduction in tool diameter with respect to increasing machining time. Further, from the experimental verification, it was found that the deviations in the tool diameters were within 9 % of the theoretical predictions.     

A Study on Process Parameters of Ultrasonic Assisted Micro EDM Based on Taguchi Method

Experimental investigation of ultrasonic assisted micro electro discharge machining was performed by introducing ultrasonic vibration to workpiece. The Taguchi experimental design has been applied to investigate the optimal combinations of process parameters to maximize the material removal rate and minimize the tool wear. Analysis of variance (ANOVA) was performed and signal-to-noise (S/N) ratio was determined to know the level of importance of the machining parameters. Based on ANOVA, ultrasonic vibration at 60% of the peak power with capacitance of 3300 PF was found to be significant for best MRR. The machining time plays a significant role in the tool wear. The results were confirmed experimentally at 95% confidence interval.     

The Visceral City and the Theatre of Fear

Over the last decade in India, intensive urbanisation, with the aid of the mass media, has unleashed a new culture of fear. Widespread access to TV, text messaging and the Internet have heightened and played upon individuals' anxieties. Ravi Sundaram describes how in Delhi, in particular, the media provided the catalyst for ‘mass hysteria’ and ‘psychosis’ during the summer of 2002. Copyright © 2007 John Wiley & Sons, Ltd.     

LSPR-based colorimetric biosensing for food quality and safety

Ensuring consistently high quality and safety is paramount to food producers and consumers alike. Wet chemistry and microbiological methods provide accurate results, but those methods are not conducive to rapid, onsite testing needs. Hence, many efforts have focused on rapid testing for food quality and safety, including the development of various biosensors. Herein, we focus on a group of biosensors, which provide visually recognizable colorimetric signals within minutes and can be used onsite. Although there are different ways to achieve visual color-change signals, we restrict our focus on sensors that exploit the localized surface plasmon resonance (LSPR) phenomenon of metal nanoparticles, primarily gold and silver nanoparticles. The typical approach in the design of LSPR biosensors is to conjugate biorecognition ligands on the surface of metal nanoparticles and allow the ligands to specifically recognize and bind the target analyte. This ligand–target binding reaction leads to a change in color of the test sample and a concomitant shift in the ultraviolet-visual absorption peak. Various designs applying this and other signal generation schemes are reviewed with an emphasis on those applied for evaluating factors that compromise the quality and safety of food and agricultural products. The LSPR-based colorimetric biosensing platform is a promising technology for enhancing food quality and safety. Aided by the advances in nanotechnology, this sensing technique lends itself easily for further development on field-deployable platforms such as smartphones for onsite and end-user applications.     

Understanding Substrate Selectivity of Phoslactomycin Polyketide Synthase by Using Reconstituted in Vitro Systems

Polyketide synthases (PKSs) use simple extender units to synthesize complex natural products. A fundamental question is how different extender units are site-specifically incorporated into the growing polyketide. Here we established phoslactomycin (Pn) PKS, which incorporates malonyl- and ethylmalonyl-CoA, as an in vitro model to study substrate specificity. We combined up to six Pn PKS modules with different termination sites for the controlled release of tetra-, penta- and hexaketides, and challenged these systems with up to seven different extender units in competitive assays to test for the specificity of Pn modules. While malonyl-CoA modules of Pn PKS exclusively accept their natural substrate, the ethylmalonyl-CoA module PnC tolerates different α-substituted derivatives, but discriminates against malonyl-CoA. We show that the ratio of extender transacylation to hydrolysis controls incorporation in PnC, thus explaining site-specific selectivity and promiscuity in the natural context of Pn PKS.     

A New Supervised Clustering Framework Using Multi Discriminative Parts and Expectation–Maximization Approach for a Fine-Grained Animal Breed Classification (SC-MPEM)

Neural Processing Letters - Fine-grained image classification is active research in the field of computer vision. Specifically, animal breed classification is an arduous task due to the challenges...     

Growth of AlxGa1−x as by metalorganic chemical vapor deposition using trimethylgallium and trimethylamine alane

High-quality Al_xGa_1−xAs layers with aluminum arsenide contentx up to 0.34 have been grown in a low pressure metalorganic chemical vapor deposition (MOCVD) system using trimethylgallium (TMG), trimethylamine alane (TMAA) and arsine. The carbon content in these films depended on growth conditions but was in general lower than in those obtained with trimethylaluminum (TMA) instead of TMAA in the same reactor under similar conditions. Unlike TMA grown layers, the TMAA grown Al_xGa_1−xAs layers, (grown at much lower temperature—down to 650° C), exhibited room temperature photolu-minescence (PL). Low temperature (25 K) PL from these films showed sharp bound exciton peaks with a line width of 5.1 meV for Al_0.25Ga_0.75As. A 39 period Al_0.28Ga_0.72As (5.5 nm)/GaAs (8.0 nm) superlattice grown at 650° C showed a strong PL peak at 25 K with a line width of 5.5 meV attesting to the high quality of these layers.