Stationary and Progressive Phenotypes Caused by the p.G90D Mutation in Rhodopsin Gene

Mutations in rhodopsin gene (RHO) are a frequent cause of retinitis pigmentosa (RP) and less often, congenital stationary night blindness (CSNB). Mutation p.G90D has previously been associated with CSNB based on the examination of one family. This study screened 60 patients. Out of these 60 patients, 32 were affected and a full characterization was conducted in 15 patients. We described the clinical characteristics of these 15 patients (12 male, median age 42 years, range 8–71) from three families including visual field (Campus Goldmann), fundus autofluorescence (FAF), optical coherence tomography (OCT) and electrophysiology. Phenotypes were classified into four categories: CSNB (N = 3, 20%) sector RP (N = 3, 20%), pericentral RP (N = 1, 6.7%) and classic RP (N = 8, 53.3% (8/15)). The phenotypes were not associated with family, sex or age (Kruskal–Wallis, p > 0.05), however, cystoid macular edema (CME) was observed only in one family. Among the subjects reporting nyctalopia, 69% (22/32) were male. The clinical characteristics of the largest p.G90D cohort so far showed a large frequency of progressive retinal degeneration with 53.3% developing RP, contrary to the previous report.     

Evaluation of the quality of collaboration between the client and the therapist in phobia treatments

A growing number of empirical studies evaluate the influence of Mental Health (MH) technology on the clinical effectiveness, the therapeutic relationship (i.e., therapeutic alliance), and usability issues. However, to the authors’ knowledge, no studies have yet been performed regarding the influence of technology on the therapeutic process in terms of collaboration. This study evaluates the quality of collaboration between the client and therapist in Augmented Reality Exposure Therapy (ARET) context and the traditional, In Vivo Exposure Therapy (IVET) context with the Therapeutic Collaborative Scale (TCS). Twenty participants received an intensive session of cognitive behavioral therapy in either a technology-mediated therapeutic context or in a traditional therapeutic context. The results indicate that both therapeutic conditions show high collaboration scores. However, the asymmetry of roles between the therapist and the client under both conditions were detected. Also, a greater level of distraction was observed for therapists in ARET, which affected the quality of the therapists’ involvement in the therapeutic session. The implications of these results are discussed.     

Towards the automatic detection of spontaneous agreement and disagreement based on nonverbal behaviour: A survey of related cues,databases, and tools

While detecting and interpreting temporal patterns of nonverbal behavioural cues in a given context is a natural and often unconscious process for humans, it remains a rather difficult task for computer systems. Nevertheless, it is an important one to achieve if the goal is to realise a naturalistic communication between humans and machines. Machines that are able to sense social attitudes like agreement and disagreement and respond to them in a meaningful way are likely to be welcomed by users due to the more natural, efficient and human‐centred interaction they are bound to experience. This paper surveys the nonverbal behavioural cues that could be present during displays of agreement and disagreement; discusses a number of methods that could be used or adapted to detect these suggested cues; lists some publicly available databases these tools could be trained on for the analysis of spontaneous, audiovisual instances of agreement and disagreement, it examines the few existing attempts at agreement and disagreement classification, and finally discusses the challenges in automatically detecting agreement and disagreement.     

Intelligent forest fire monitoring system

This paper presents iForestFire, an Environmental Monitoring Information System for forest fire protection. The system is composed of several components, each having a particular function. Automatic fire detection is a crucial component of the system. It is based on various complex image processing algorithms. Complexity of the system also emerges from integration, based on multi agent technology, of different environment information. The presented system contributes to the environment protection and is in use in Croatia for several years.     

Spectroscopic evaluation of the colour play effect of thermochromic liquid crystal printing inks

Colour changes of a thermochromic liquid crystal (TLC) ink printed on a black substrate are clearly observed by the naked eye, and therefore it is reasonable to expect that every approach recommended for colour measurements of samples with solid colour would be appropriate. In addition, the published spectroscopic analyses of TLC inks do not provide any guidance or limitations for the measurement geometry. On the other hand, our study demonstrates that spectroscopic measurements of such samples are not so straightforward. When using the most simple and commonly used measurement equipment with a directional illumination and viewing approach, the temperature-dependent spectral features resolve in a narrow spectral region that gives only a small part of the colour play effect. Detection of the entire effect requires the use of integrating spheres, which spatially integrate the radiant flux reflected on a sample in any direction; better results are obtained by spheres of larger diameter. The comparision of spectroscopic and colorimetric results obtained when different measurement geometries are applied on the same sample may demonstrate certain properties of the liquid crystal material inside microcapsules and help in understanding some details of the optical properties of the ink layer.     

Shear bond strength to dentine of dental adhesives containing hydroxyapatite nano-fillers

The aim of this study was to compare the effect of hydroxyapatite (HAP) nano-rods (HAP_rods) and HAP nano-sticks (HAP_sticks) added to commercial adhesives on the macro-shear bond strength (SBS) to dentine and morphology of the adhesive–dentine interface. HAP was added to Single Bond Universal (SBU, 3M ESPE) and Te-Econom Bond (TeE; Ivoclar Vivadent), in the form of water suspensions to avoid agglomeration of nano-particles and to achieve HAP concentrations of 0.5, 1.0 and 1.5 wt%. Following a ‘total-etch’ or a ‘self-etch’ protocol, the adhesives were applied to flat dentine surface of 162 intact human, third molars (N = 6/group). Composite (Z250, 3M ESPE) was built-up using a split stainless steel mould, 3 mm in diameter. SBS was tested using a universal testing machine at 1 mm/min until fracture. Data were statistically analysed using two-way and one-way analysis of variance with Tukey’s post-test (α = 0.05). HAP_rods had no significant effect on SBS of the tested adhesives while HAP_sticks improved bond strength to dentine only of adhesives applied following the ‘total-etch’ total-etch protocol. SBS values of SBU containing 1% HAP_sticks (15.10 ± 2.96 MPa) and TeE containing 0.5% HAP_sticks applied following the total-etch protocol (12.96 ± 4.48 MPa) were higher than those of their respective control groups (10.36 ± 2.68 and 7.97 ± 3.64 MPa). Samples with higher SBS showed more ‘mixed’ failures. HAP nano-fillers may improve bond strength of total-etch adhesives without an adverse effect on adhesive dispersion on dentine and its ability to infiltrate dentinal tubules.     

Microbes versus microbes: control of pathogens in the food chain

Foodborne illness continues as a considerable threat to public health. Despite improved hygiene management systems and increased regulation, pathogenic bacteria still contaminate food, causing sporadic cases of illness and disease outbreaks worldwide. For many centuries, microbial antagonism has been used in food processing to improve food safety. An understanding of the mode of action of this microbial antagonism has been gained in recent years and potential applications in food and feed safety are now being explored. This review focuses on the potential opportunities presented, and the limitations, of using microbial antagonism as a biocontrol mechanism to reduce contamination along the food chain; including animal feed as its first link. © 2014 Society of Chemical Industry