Study of an annular laser beam based axially-fed powder cladding process

An annular laser beam based powder cladding head, which enables an axial powder feeding and variation of the laser beam intensity distribution (LBID) on the workpiece surface is presented. The influence of typical LBIDs, including Ring, Tophat(?), Tophat(+), and Gaussian-like, on a cladding process has been characterized based on the process and melt pool visualization, powder catchment efficiency, clad layer geometry, and porosity. The results showed that the most stable process without plasma formation but with low dilution and porosity of the clad layer can be achieved within the range from a Ring to a Tophat(?) LBID. Additionally, axial powder feeding results in a high powder catchment efficiency above 80%.     

Decreasing resistances of membrane-electrode assemblies containing hydrocarbon-based ionomers for improving their anodic performances

To improve methanol-oxidation performances of membrane-electrode assemblies composed of a hydrocarbon-based ionomers, the resistances involved in the reaction were decreased. Electrochemical impedance spectroscopy revealed that the proton-conductive resistance (R_i) in the anode was decreased from 0.54 to 0.40 Ω cm² by increasing a loading ratio of platinum-ruthenium to carbon support of anode catalyst from 54 to 73 wt.%. In addition, R_i was decreased to be 0.25 Ω cm² by increasing ion-exchange capacity (IEC) of the ionomer from 1.4 to 2.9 mequiv. g⁻¹. Consequently, the polarization resistance of the anode was significantly decreased, in turn, increasing current density of methanol oxidation at the potential of 0.45 V from 0.110 to 0.244 A cm⁻².     

Magnetic properties and phase diagram of Ni50Mn50−xGax ferromagnetic shape memory alloys

We present the magnetic properties and the magnetic phase diagram of Ni₅₀Mn_50?xGa_x ferromagnetic shape memory alloys across a wide concentration range. Martensitic transformation, intermediate transformation, B2–L2₁ order–disorder transformation, Néel and Curie temperatures are determined for the prepared samples. The martensitic transformation temperature decreases with increasing Ga concentration and bends two times when crossing the Curie temperature and the intermediate-phase transformation temperature. Spontaneous magnetization and its composition dependence were also investigated. Composition dependence of the transformation temperatures and the spontaneous magnetization in the martensite phase of Ni₅₀Mn_50?xGa_x are compared with those of Ni₅₀Mn_50?xIn_x and Ni₅₀Mn_50?xSn_x, revealing a similarity in the NiMn-based alloy systems.     

Identification of SYS1 as a Host Factor Required for Shiga Toxin-Mediated Cytotoxicity in Vero Cells

Shiga toxin (STx) or Vero toxin is a virulence factor produced by enterohemorrhagic Escherichia coli. The toxin binds to the glycosphingolipid globotriaosylceramide (Gb3) for its entry, and causes cell death by inhibiting ribosome function. Previously, we performed a loss-of-function screen in HeLa cells using a human CRISPR knockout (KO) library and identified various host genes required for STx-induced cell death. To determine whether this library targeted to the human genome is applicable to non-human primate cells and to identify previously unrecognized factors crucial for STx-induced cell death, we herein performed a similar screen in the African green monkey kidney-derived Vero C1008 subline. Many genes relevant to metabolic enzymes and membrane trafficking were enriched, although the number of enriched genes was less than that obtained in the screening for HeLa cells. Of note, several genes that had not been enriched in the previous screening were enriched: one of these genes was SYS1, which encodes a multi-spanning membrane protein in the Golgi apparatus. In SYS1 KO Vero cells, expression of Gb3 and sphingomyelin was decreased, while that of glucosylceramide and lactosylceramide was increased. In addition, loss of SYS1 inhibited the biosynthesis of protein glycans, deformed the Golgi apparatus, and perturbed the localization of trans-Golgi network protein (TGN) 46. These results indicate that the human CRISPR KO library is applicable to Vero cell lines, and SYS1 has a widespread effect on glycan biosynthesis via regulation of intra-Golgi and endosome–TGN retrograde transports.     

Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer

Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases.     

Differential Effects of Fkbp4 and Fkbp5 on Regulation of the Proopiomelanocortin Gene in Murine AtT-20 Corticotroph Cells

The hypothalamic-pituitary-adrenal axis is stimulated in response to stress. When activated, it is suppressed by the negative feedback effect of glucocorticoids. Glucocorticoids directly inhibit proopiomelanocortin (Pomc) gene expression in the pituitary. Glucocorticoid signaling is mediated via glucocorticoid receptors, 11β-hydroxysteroid dehydrogenases, and the FK506-binding immunophilins, Fkbp4 and Fkbp5. Fkbp4 and Fkbp5 differentially regulate dynein interaction and nuclear translocation of the glucocorticoid receptor, resulting in modulation of the glucocorticoid action. Here, we explored the regulation of Fkbp4 and Fkbp5 genes and their proteins with dexamethasone, a major synthetic glucocorticoid drug, in murine AtT-20 corticotroph cells. To elucidate further roles of Fkbp4 and Fkbp5, we examined their effects on Pomc mRNA levels in corticotroph cells. Dexamethasone decreased Pomc mRNA levels as well as Fkpb4 mRNA levels in mouse corticotroph cells. Dexamethasone tended to decrease Fkbp4 protein levels, while it increased Fkpb5 mRNA and its protein levels. The dexamethasone-induced decreases in Pomc mRNA levels were partially canceled by Fkbp4 knockdown. Alternatively, Pomc mRNA levels were further decreased by Fkbp5 knockdown. Thus, Fkbp4 contributes to the negative feedback of glucocorticoids, and Fkbp5 reduces the efficiency of the glucocorticoid effect on Pomc gene expression in pituitary corticotroph cells.     

New catalytic technologies in Japan

Recent trends in R&D of catalytic technology in Japan (cleaner and more efficient production, environmental catalysts, and recycling processes) are overviewed and examples of recent achievements are listed. Examples are then described as zeolite-catalyzed organic reactions recently commercialized and expected to be commercialized: hydration of cyclohexene, synthesis of pyridine derivatives and gas-phase Beckmann rearrangement of cyclohexanone oxime. Finally, as an example of environmental catalysts, the NO_x storage–reduction type three-way automobile catalyst, is introduced.     

Gasification characteristics of organic waste by molten salt

Recently, along with the growth in economic development, there has been a dramatic accompanying increase in the amount of sludge and organic waste. The disposal of such is a significant problem. Moreover, there is also an increased in the consumption of electricity along with economic growth. Although new energy development, such as fuel cells, has been promoted to solve the problem of power consumption, there has been little corresponding promotion relating to the disposal of sludge and organic waste. Generally, methane fermentation comprises the primary organic waste fuel used in gasification systems. However, the methane fermentation method takes a long time to obtain the fuel gas, and the quality of the obtained gas is unstable. On the other hand, gasification by molten salt is undesirable because the molten salt in the gasification gas corrodes the piping and turbine blades. Therefore, a gasification system is proposed by which the sludge and organic waste are gasified by molten salt. Moreover, molten carbonate fuel cells (MCFC) are needed to refill the MCFC electrolyte volatilized in the operation. Since the gasification gas is used as an MCFC fuel, MCFC electrolyte can be provided with the fuel gas. This paper elucidates the fundamental characteristics of sludge and organic waste gasification. A crucible filled with the molten salt comprising 62 Li₂CO₃/38 K₂CO₃, is installed in the reaction vessel, and can be set to an arbitrary temperature in a gas atmosphere. In this instance, the gasifying agent gas is CO₂. Sludge or the rice is supplied as organic waste into the molten salt, and is gasified. The chemical composition of the gasification gas is analyzed by a CO/CO₂ meter, a HC meter, and a SO_x meter gas chromatography. As a result, although sludge can generate CO and H₂ near the chemical equilibrium value, all of the sulfur in the sludge is not fixed in the molten salt, because the sludge floats on the surface of the carbonate by the specific gravity of sludge lighter than the carbonate, and is not completely converted into CO and H₂. Moreover, the rice also shows good characteristics as a gasifying agent. Consequently, there is high expectation to using the organic waste as a molten salt gasifying agent. However, this requires lengthening the contact time between the organic waste and the molten salt.     

A novel l-amino acid ligase from bacillus licheniformis

l-Amino acid alpha-ligase (EC 6.3.2.28) catalyzed formation of alpha-peptide bond in unprotected l-amino acids in an ATP-dependent manner. BL00235 gene in Bacillus licheniformis NBRC12200 coded as a new l-amino acid ligase. BL00235 substrate specificity was strict; only methionine or leucine was acceptable as dipeptide N-terminal residues.     

Structural Change of Maize Starch Granules by Ball-mill Treatment

Effect of ball-mill treatment on physical properties and molecular change of maize starch granule was investigated. Ball-mill treatment was done by rotary type mill, and species of maize starch are normal, waxy and high amylose (amylo). Running time of the treatment is 0–320 h. Starch granules loss smoothness on surface and became rough, even though their changing speed was different among the three species. But, they retained whole figure and size after 320 h treatment in the all cases. Amylase susceptivity and water absorption activity were measured. Structural change of starch components was compared among the three species with X-ray diffraction, DSC and GPC. High Performance An-ion Exchange Chromatography (HPAEC) pattern of debranched sample treated with ball-mill for 320 h showed that formation of very short chain in amylopectin is little. ¹³C solid-NMR spectra suggest that disruption of molecules of amylopectin and amylose with ball-mill might occur at their glycosidic linkage. However, very slight radical was observed by Electron Spin Resonance spectroscopy (ESR) in the case of 320 h sample.