Complete single mode wavelength coverage over 40 nm with a superstructure grating DBR laser

The spectral characteristics of a widely tunable super structure grating distributed Bragg reflector (SSG-DBR) laser are investigated and the design considerations of the grating reflectors are given. By systematically adjusting the control currents of the two reflector sections and the phase section it is experimentally shown that every wavelength in an interval of 40 nm can be reached with a side-mode suppression usually better than 30 dB     

A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food

The beige mutation is a murine autosomal recessive disorder, resulting in hypopigmentation, bleeding and immune cell dysfunction. The gene defective in beige is thought to be a homologue of the gene for the human disorder Chediak-Higashi syndrome. We have identified the murine beige gene by in vitro complementation and positional cloning, and confirmed its identification by defining mutations in two independent mutant alleles. The sequence of the beige gene message shows strong nucleotide homology to multiple human ESTs, one or more of which may be associated with the Chediak-Higashi syndrome gene. The amino acid sequence of the Beige protein revealed a novel protein with significant amino acid homology to orphan proteins identified in Saccharomyces cerevisiae, Caenorhabditis elegans and humans.     

Direct dopamine D2-receptor-mediated modulation of arachidonic acid release in transfected CHO cells without the concomitant administration of a Ca2+-mobilizing agent

In CHO cells transfected with the rat dopamine D2 receptor (long isoform), administration of dopamine per se elicited a concentration-dependent increase in arachidonic acid (AA) release. The maximal effect was 197% of controls (EC50=25 nM). The partial D2 receptor agonist, (-)-(3-hydroxyphenyl)-N-n-propylpiperidine [(-)-3-PPP], also induced AA release, but with somewhat lower efficacy (maximal effect: 165%; EC50=91 nM). The AA-releasing effect of dopamine was counteracted by pertussis toxin, by the inhibitor of intracellular Ca2+ release, 8-(N N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8), by excluding calcium from the medium, by the phospholipase A2 (PLA2) inhibitor, quinacrine, and by long-term pretreatment with the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA). In addition, it was antagonized by the D2 antagonists, raclopride and (-)-sulpiride--but not by (+)-sulpiride--and absent in sham-transfected CHO cells devoid of D2 receptors. The results obtained contrast to the previous notion that dopamine and other D2 receptor agonists require the concomitant administration of calcium-mobilizing agents such as ATP, ionophore A-23187 (calcimycin), thrombin, and TRH, to influence AA release from various cell lines.     

Phase Equilibria and Thermodynamics in the Al2O3–SiO2 System—Modeling of Mullite and Liquid

The Al₂O₃–SiO₂ system has been reassessed using a solution model for mullite extending from sillimanite to a hypothetical state of alumina. The property of sillimanite, to be used to describe one of the end-members, was extracted from an analysis of the T – P phase diagram for Al₂SiO₅ polymorphs. It was possible to represent the information on the range of stability of mullite, including some showing that mullite extends to higher SiO₂ contents than represented by the composition of 3:2 mullite. An attempt was made to model the liquid with the ionic two-sublattice model using a new species AlO₂⁻¹. The pressure dependence of Al₂SiO₅ polymorphs was optimized by a new model recently implemented in Thermo-Calc.     

A dual-affinity gene fusion system to express small recombinant proteins in a soluble form: expression and characterization of protein A deletion mutants

A novel gene fusion system to express and purify small recombinantproteins in Escherichia coli has been constructed. The conceptallows for affinity purification of soluble gene products bysequential albumin- and Zn²+-affinity chromatography. The dual-affinitysystem is well suited for expression of unstable proteins asonly full-length protein is obtained after purification andproteins gain proteolytic stability in the fusion protein. Herewe show that the dual-affinity approach can be used for theexpression of various unstable derivatives of a single IgG-bindingdomain based on staphylococcal protein A. Analysis of the proteolyticstabilities and the IgG-binding properties of the differentmutant proteins suggest that the model for the structure ofan IgG-binding domain must be re-evaluated.     

分块云计算

Jimmy Nilsson在过去数年间观察到一种架构风格正慢慢流行,通过对过去数年的总结,对过往的痛苦自省,他将这种新的架构风格做了总结。     

A mathematical model for estimation of sulphide capacities of multi-component slags

The present work presents mathematical model for the estimation of sulphide capacities. The sulphide capacity, C_S is expressed as (1) where ΔG⁰ is the standard Gibbs energy change for the reaction, (2) ξ, being a function of both temperature and composition, is characterized by the system under consideration. By optimizing ξ, the sulphide capacities could be estimated as functions of temperature and composition. The slag composition is described using a modified Temkin approach, where complex polymeric ions are considered as dissociated to simple species. The model has been applied to the binary systems CaO-SiO₂, MnO-SiO₂ as well as the CaO-MnO-SiO₂ ternary system. The results show that reliable predictions of the sulphide capacities can be obtained using the present model.     

Perceived production complexity – understanding more than parts of a system

To successfully manage today’s complex production systems it is essential to study operators’ perception of the system. The paper presents perceived production complexity assessed at seven manufacturing companies with the CompleXity Index (CXI) method. While other methods have measured product variants, layout, work content, tools and information items, CXI combines them into three areas. These three complexity areas (Station design, Work variance and Disturbance handling) help to create an understanding of the complex system by visualising how they contribute to complexity, and show the need to support the operators’ ability to handle the varying work.