Immunoglobulin 1 (IgG1) Fc-glycosylation profiling of anti-citrullinated peptide antibodies from human serum

In several autoimmune disorders, including rheumatoid arthritis (RA), autoantibodies are thought to be the driving force of pathogenicity. Glycosylation of the Fc-part of human Igs is known to modulate biological activity. Hitherto, glycosylation of human IgG-Fc has been analyzed predominantly at the level of total serum IgG, revealing reduced galactosylation in RA. Given the pathogenic relevance of autoantibodies in RA, we wished, in the present study, to address the question whether distinct Fc-glycosylation features are observable at the level of antigen-specific IgG subpopulations. For this purpose, we have developed a method for the microscale purification and Fc-glycosylation analysis of anti-citrullinated peptide antibodies (ACPA). ACPA represent a group of autoantibodies that occur with unique specificity in RA patients. Their presence is associated with increased inflammatory disease activity and rapid joint destruction. Results indicate that ACPA of the IgG1 subclass vary considerably from total serum IgG1 with respect to Fc-galactosylation, with galactosylation being higher on ACPA than on serum IgG1 for some patients, while other patients show higher galactosylation on serum IgG1 than on ACPA. Using this method, studies can be performed on the biological and clinical relevance of ACPA glycosylation within RA patient cohorts.     

An element-based formulation for ES-FEM and FS-FEM models for implementation in standard solid mechanics finite element codes for 2D and 3D static analysis

Edge-based and face-based smoothed finite element methods (ES-FEM and FS-FEM, respectively) are modified versions of the finite element method allowing to achieve more accurate results and to reduce sensitivity to mesh distortion, at least for linear elements. These properties make the two methods very attractive. However, their implementation in a standard finite element code is nontrivial because it requires heavy and extensive modifications to the code architecture. In this article, we present an element-based formulation of ES-FEM and FS-FEM methods allowing to implement the two methods in a standard finite element code with no modifications to its architecture. Moreover, the element-based formulation permits to easily manage any type of element, especially in 3D models where, to the best of the authors' knowledge, only tetrahedral elements are used in FS-FEM applications found in the literature. Shape functions for non-simplex 3D elements are proposed in order to apply FS-FEM to any standard finite element.     

Modelling and analysis of the dynamics of a tilting three-wheeled vehicle

To understand the handling behaviour of a three-wheeled tilting vehicle, models of the vehicle with different level of detail, corresponding to specific fields of investigation, have been developed. Then the proposed kinematics of the three-wheeler are assessed and optimized with respect to desired dynamic properties by applying a detailed multibody system model. The partially unstable nature of the motion of the vehicle suggests the application of an analytically derived, simplified model, to allow for focusing on stability aspects and steady-state handling properties. These investigations reveal the necessity of employing a steer-by-wire control system to support the driver by stabilizing the motion of the vehicle. Thus, an additional basic vehicle model is derived for control design, and an energy-efficient control strategy is presented. Numerical simulation results demonstrate the dynamic properties of the optimized kinematics and the control system, approved by successful test runs of a prototype.     

On generic context lemmas for higher-order calculi with sharing

This paper proves several generic variants of context lemmas and thus contributes to improving the tools for observational semantics of deterministic and non-deterministic higher-order calculi that use a small-step reduction semantics. The generic (sharing) context lemmas are provided for may- as well as two variants of must-convergence, which hold in a broad class of extended process- and extended lambda calculi, if the calculi satisfy certain natural conditions. As a guide-line, the proofs of the context lemmas are valid in call-by-need calculi, in call-by-value calculi if substitution is restricted to variable-by-variable and in process calculi like variants of the $\pi$-calculus. For calculi employing beta-reduction using a call-by-name or call-by-value strategy or similar reduction rules, some iu-variants of ciu-theorems are obtained from our context lemmas. Our results reestablish several context lemmas already proved in the literature, and also provide some new context lemmas as well as some new variants of the ciu-theorem. To make the results widely applicable, we use a higher-order abstract syntax that allows untyped calculi as well as certain simple typing schemes. The approach may lead to a unifying view of higher-order calculi, reduction, and observational equality.     

The effect of the microfluidic diodicity on the efficiency of valve-less rectification micropumps using Lattice Boltzmann Method

The efficiency of the valve-less rectification micropump depends primarily on the microfluidic diodicity (the ratio of the backward pressure drop to the forward pressure drop). In this study, different rectifying structures, including the conventional structures (nozzle/diffuser and Tesla structures), were investigated at very low Reynolds numbers (between 0.2 and 60). The rectifying structures were characterized with respect to their design, and a numerical approach was illustrated to calculate the diodicity for the rectifying structures. In this study, the microfluidic diodicity was evaluated numerically for different rectifying structures including half circle, semicircle, heart, triangle, bifurcation, nozzle/diffuser, and Tesla structures. The Lattice Boltzmann Method (LBM) was utilized as a numerical method to simulate the fluid flow in the microscale. The results suggest that at very low Reynolds number flow, rectification and multifunction micropumping may be achievable by using a number of the presented structures. The results for the conventional structures agree with the reported results.     

A visual language for modeling multiple perspectives of business process compliance rules

A fundamental challenge for enterprises is to ensure compliance of their business processes with imposed compliance rules stemming from various sources, e.g., corporate guidelines, best practices, standards, and laws. In general, a compliance rule may refer to multiple process perspectives including control flow, time, data, resources, and interactions with business partners. On one hand, compliance rules should be comprehensible for domain experts who must define, verify, and apply them. On the other, these rules should have a precise semantics to avoid ambiguities and enable their automated processing. Providing a visual language is advantageous in this context as it allows hiding formal details and offering an intuitive way of modeling the compliance rules. However, existing visual languages for compliance rule modeling have focused on the control flow perspective so far, but lack proper support for the other process perspectives. To remedy this drawback, this paper introduces the extended Compliance Rule Graph language, which enables the visual modeling of compliance rules with the support of multiple perspectives. Overall, this language will foster the modeling and verification of compliance rules in practice.