Effects of high‐pressure homogenisation on physicochemical characteristics of partially skimmed milk

The changes in partially skimmed milk (0.5% fat) physicochemical properties and proteins after high‐pressure homogenisation (HPH) at 100, 200 and 300 MPa were investigated. Processing parameters and changes in pH, ethanol precipitation stability, lightness, whey protein denaturation, hydrophobicity and viscosity were evaluated. No significant differences were found between milk pH and nonprotein nitrogen content before and after HPH. Ethanol stability, lightness and hydrophobicity increased when pressure was increased from 100 MPa to 300 MPa. Whey protein denaturation, evaluated through noncasein nitrogen, occurred only at 200 to 300 MPa, and viscosity increased just at 300 MPa. Therefore, HPH changed some milk physicochemical characteristics, mainly those related to protein content. These results highlight that HPH processing is a promising technology to improve partially skimmed milk mouth feel being suitable for dairy products manufacturing.     

Synthesis and Photopolymerization of Tween 20 Methacrylate/N-vinyl-2-Pyrrolidone Blends

Poly(oxyethylene 20 sorbitan) monolaurate (Tween® 20) methacrylates were synthesized by coupling methacryloyl chloride (MeOCl) to Tween 20 in the presence of 4-(N,N-dimethylamino) pyridine, using THF as a solvent, in order to investigate their suitability as precursors for photopolymerizable hydrogels in tissue engineering applications. The degree of substitution could be controlled by adjusting the molar ratio of MeOCl and Tween 20, giving three different monomers: Tween 20 monomethacrylate, Tween 20 dimethacrylate and Tween 20 trimethacrylate. Combined ¹ H NMR and MALDI-TOF MS confirmed these monomers to be of high purity and to have polydispersities less than 1.3. It was shown that aqueous solutions of the monomers were photoactive, all the methacrylate groups reacting within 30 minutes exposure to a UV light intensity of 145 mW/cm². Aqueous Tween 20 trimethacrylate was then combined with N-vinyl-2-pyrrolidone (NVP), giving tough copolymer hydrogels on photopolymerization, whose swelling ratios and swelling rates could be tuned by varying the Tween 20 trimethacrylate content. The use of a flexible spacer with a multifunctional monomer gives a permanent three-dimensional network, whilst maintaining degrees of swelling of between 60 and 85%, with potential for a wide range of biological and non-biological applications.     

Liar liar,pants on fire; or how to use subjective logic and argumentation to evaluate information from untrustworthy sources

This paper presents a non-prioritized belief change operator, designed specifically for incorporating new information from many heterogeneous sources in an uncertain environment. We take into account that sources may be untrustworthy and provide a principled method for dealing with the reception of contradictory information. We specify a novel Data-Oriented Belief Revision Operator, that uses a trust model, subjective logic, and a preference-based argumentation framework to evaluate novel information and change the agent’s belief set accordingly. We apply this belief change operator in a collaborative traffic scenario, where we show that (1) some form of trust-based non-prioritized belief change operator is necessary, and (2) in a direct comparison between our operator and a previous proposition, our operator performs at least as well in all scenarios, and significantly better in some.     

Carbonation durability of blended cement pastes used for waste encapsulation

Blended cement pastes are currently used for encapsulation of low level and intermediate level nuclear waste in the UK. However, there is still little information on the long-term durability of those mixes to some chemical attacks. Accelerated testing may predict the long-term durability or at least help the selection of more durable formulations. In this work, blended blastfurnace slag (BFS)/Portland cement (OPC) pastes containing 60, 75 and 90% BFS and pulverised fuel ash (PFA)/OPC pastes with 40, 55 and 75% PFA were cured at 20 and 60°C for 90 days then submitted to natural and accelerated carbonation (5% CO₂). The effects of the curing temperature as well as the OPC replacement level on the carbonation ratio are presented. Results showed a good correlation between natural and accelerated carbonation for the pastes studied. Carbonation was found to be governed by the amount of calcium hydroxide available in the mixes before the process started.     

Characterization of Microencapsulated Rosemary Essential Oil and Its Antimicrobial Effect on Fresh Dough

This study aimed to characterize rosemary essential oil particles obtained by spray-drying and to evaluate their antimicrobial activity. Measurements of mycelial growth of Penicillium and Aspergillus fungi, isolated from fresh dough, were made applying oil concentrations of 1.0, 5.0, 10.0, 20.0, and 50.0 μL/mL. Fungi and yeast counts in the fresh dough submitted to the control (no oil), pure oil, and microencapsulated oil treatments were also conducted. The microcapsules showed 50 % oil retention after drying, and the major oil components identified were 1,8-cineole (29.0 %), camphor (26.6 %), and α-pinene (10.6 %). The analysis of the particles revealed surfaces without fissures, with a mean particle size of 12.2 μm and presenting an amorphous structure. The growth inhibiting effect of Penicillium sp. fungus, compared to the control, was verified at concentrations of 1.0, 5.0, and 10.0 μL/mL rosemary essential oil, which did not differ among them. For Aspergillus sp., the application of 10.0 μL/mL oil provided greater inhibition compared to 1.0 and 5.0 μL/mL. Complete inhibition occurred with the application of 50.0 μL/mL for both fungal genera tested. At 8 days of dough storage at 25 °C, a decrease of at least 0.7 and 1.5 log cycles of fungal growth was observed in the dough with pure oil and that with microencapsulated oil, respectively, relative to the control. The microencapsulation process retained the antimicrobial property of rosemary essential oil and provided further extension of this activity over time when applied to fresh dough.     

Relationships between MODIS phenological metrics,topographic shade,and anomalous temperature patterns in seasonal deciduous forests of south Brazil

We investigated the relationships between 11 phenological metrics, topographic shade, and anomalous temperature patterns detected using wavelet analysis in seasonal deciduous forests of south Brazil. To obtain the metrics, we applied the TIMESAT algorithm to the enhanced vegetation index (EVI) from Moderate Resolution Imaging Spectroradiometer (MODIS)/Terra. MODIS acquires data from the study area under a large seasonal amplitude in the solar zenith angle (SZA). We evaluated the effect of topography on phenological metrics by correlating the metrics with shaded relief values. To analyse the inter-annual phenological metric variations with anomalous and regular temperature patterns, we calculated standard anomalies for each metric. Finally, we established relationships between the metrics and the minimum, maximum, and mean temperatures from growing seasons that spanned over 10 seasonal cycles between 2002 and 2012. The correlation results with shaded relief showed that the left (LD) and right derivative (RD), small integral (SInt), seasonal amplitude (SA), base level (BL), and maximum VI value (MV) were sensitive to topographic effects. The seasonal cycles with the highest temperatures in the growing season (2006/2007 and 2009/2010) exhibited a delay at the end of the cycle and a higher interval of duration and productivity, which was indicated by the positive standard anomalies for end of season (EOS), length of season (LOS), large integral (LInt), and SInt. We observed a different result for the lowest temperature cycle (2003/2004). The means for these metrics in anomalous seasons differed significantly from the metrics of other regular cycles at the 0.05 significance level using paired t-tests. Statistically significant correlations were observed between the metrics and minimum and mean temperature values of the 10 seasonal cycles.     

Anaerobic methane oxidation and aerobic methane production in an east African great lake (Lake Kivu)

We investigated CH₄ oxidation in the water column of Lake Kivu, a deep meromictic tropical lake with CH₄-rich anoxic deep waters. Depth profiles of dissolved gases (CH₄ and N₂O) and a diversity of potential electron acceptors for anaerobic CH₄ oxidation (NO₃^?, SO₄^2?, Fe and Mn oxides) were determined during six field campaigns between June 2011 and August 2014. Denitrification measurements based on stable isotope labelling experiments were performed twice. In addition, we quantified aerobic and anaerobic CH₄ oxidation, NO₃^? and SO₄^2? consumption rates, with and without the presence of an inhibitor of SO₄^2?-reducing bacteria activity. Aerobic CH₄ production was also measured in parallel incubations with the addition of an inhibitor of aerobic CH₄ oxidation. The maximum aerobic and anaerobic CH₄ oxidation rates were estimated to be 27?±?2 and 16?±?8?μmol/L/d, respectively. We observed a difference in the relative importance of aerobic and anaerobic CH₄ oxidation during the rainy and the dry season, with a greater role for aerobic oxidation during the dry season. Lower anaerobic CH₄ oxidation rates were measured in presence of molybdate in half of the measurements, suggesting the occurrence of linkage between SO₄^2? reduction and anaerobic CH₄ oxidation. NO₃^? consumption and dissolved Mn production rates were never high enough to sustain the measured anaerobic CH₄ oxidation, reinforcing the idea of a coupling between SO₄^2? reduction and CH₄ oxidation in the anoxic waters of Lake Kivu. Finally, significant rates (up to 0.37?μmol/L/d) of pelagic CH₄ production were also measured in oxygenated waters.     

H∞ control of continuous-time Markov jump linear systems with detector-based mode information†

This paper features new results on H_∞ analysis and control of linear systems with Markov jump disturbances, in a scenario of partial observations of the jump process. We consider the situations in which the jump process can only be measured through a suitable detector. A distinctive feature of the approach here is that it is general enough to encompass particular scenarios such as that of perfect information, no information (mode independent) and cluster observations of the Markov jump process. The main results, comprising a new bounded real lemma and a condition for state feedback synthesis, are expressed via linear matrix inequality-based optimisation problems. The method devised for the design of H_∞ controllers is applied to the control of an unmanned aerial vehicle model.     

Calcium dosing for the simultaneous control of biomass retention and the enhancement of fermentative biohydrogen production in an innovative fixed-film bioreactor

Biohydrogen (bioH₂) production via dark fermentation is an attractive approach to overcome the drawbacks of conventional hydrogen production methods and represents a preliminary alternative for the management of organic wastes. Fundamental studies are still required to enhance the performance of bioH₂ production systems, with emphasis on the development of novel reactor configurations. The anaerobic structured-bed reactor (ASTBR) is a recently developed configuration with great potential for bioH₂ production, although operating strategies are still required to minimize biomass washout in such systems. In this context, calcium dosing has been investigated as a strategy to enhance both biomass retention and bioH₂ production rates in the ASTBR. The present study employed varying COD/calcium ratios (4423, 2079, 1357, 1012, 884, and 632) in continuous experiments under mesophilic conditions (25 °C). Calcium dosing effectively enhanced biomass retention within the ASTBR, directly increasing the availability of metabolic energy for different metabolic pathways rather than cell synthesis. An optimal COD/calcium ratio of 1360 was mathematically estimated for bioH₂ production, which is consistent with the results obtained experimentally. The specific organic loading rate (SOLR) was better controlled at this ratio, indicating the establishment of balanced conditions in terms of substrate availability and biomass concentration. Conversely, bioH₂ production was severely impaired at COD/calcium values below and above the optimal range, most likely due to enhancement of the homoacetogenic pathway as a result of unbalanced conditions in the SOLR. Furthermore, biomass accumulation did not strongly affect the mean residence time of the ASTBR, facilitating its robust and enhanced solid retention.     

Comparison on efficiency factors of F and C types of fly ashes

Fly ashes are obtained from thermal power plants and they are pozzolanic materials, which can act as partial replacement material for both portland cement and fine aggregate. With their economical advantages and potential for improving fresh and hardened concrete performance, they have some benefits for using in concrete industry. In this study, the objective was to find the efficiency factors of Turkish C and F-type fly ashes and to compare their properties. Three different cement dosages were used (260, 320, 400 kg/m³), two different ratios (10% and 17%) of cement reduced from the control concretes and three different ratios (depending on cement reduction ratio) of fly ash were added into the mixtures. At the ages of 28 and 90 days, compressive strength, modulus of elasticity and ultrasound velocity tests were carried out. From the compressive strength results, the k efficiency factors of C and F-type fly ashes were obtained. As a result, it is seen that efficiency factors of the concrete produced by the replacement of F and C type fly ashes with cement increase with the increase in cement dosage and concrete age.