Effect of phosphate binders on oxidative stress and inflammation markers in hemodialysis patients

It has been suggested that phosphate binders may reduce the inflammatory state of hemodialysis (HD) patients. However, it is not clear whether it has any effect on oxidative stress. The objective of this study was to evaluate the effect of sevelamer hydrochloride (SH) and calcium acetate (CA) on oxidative stress and inflammation markers in HD patients. Hemodialysis patients were randomly assigned to therapy with SH (n=17) or CA (n=14) for 1 year. Before the initiation of therapy (baseline) and at 12 months, we measured in vitro reactive oxygen species (ROS) production by stimulated and unstimulated polymorphonuclear neutrophils and serum levels of tumor necrosis factor α, interleukin-10, C-reactive protein, and albumin. There was a significant reduction of spontaneous ROS production in both groups after 12 months of therapy. There was a significant decrease of Staphylococcus aureus stimulated ROS production in the SH group. There was a significant increase in albumin serum levels only in the SH group. In the SH group, there was also a decrease in the serum levels of tumor necrosis factor α and C-reactive protein. Our results suggest that compared with CA treatment, SH may lead to a reduction in oxidative stress and inflammation. Therefore, it is possible that phosphate binders exert pleiotropic effects on oxidative stress and inflammation, which could contribute toward decreasing endothelial injury in patients in HD.     

Biodiesel production from waste coconut oil by esterification with ethanol: The effect of water removal by adsorption

The production of biodiesel by esterification with ethanol using waste oil generated in the refining of coconut oil was investigated in this study. The reaction was performed with and without adsorption of water in order to verify the effect of removing water on the reaction conversion. Methanol was also evaluated as an esterification agent. For both ethanol and methanol, conversions over 99% mol were observed. Simultaneous water adsorption allowed the use of lower alcohol/oil molar ratios thus enabling better economics to a possible industrial process.     

Synthesis,characterization and evaluation of phosphorylated resins in the removal of Pb<Superscript>2+</Superscript> from aqueous solutions

Phosphinic-derivative poly(styrene-co-divinylbenzene)-based on PS–DVB copolymers with different porosity degrees have been prepared by aromatic electrophilic substitution reaction using PCl₃/AlCl₃ followed by base-promoted hydrolysis. The phosphorylation reaction was analyzed by infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), and thermogravimetry (TG/DTG). In addition, the phosphorous content of the phosphorylated copolymers was determined by spectrophotometry using the method based on sodium molybdate reactant so that the extension of that modification could be assessed. The performance of the phosphorylated resins in the extraction of Pb²⁺ from aqueous solutions in a batch system was also evaluated. The Pb²⁺ content was determined by atomic absorption spectrometry (AAS). These materials presented excellent extraction capacity under the contact time of 30 min and pH 6.     

Analysis of turbulent combustion in inert porous media

The objective of this paper is to present an extension of a simplified reaction kinetics model that, combined with a thermo-mechanical closure, entails a full-generalized turbulent combustion model for flow in porous media. In this model, one explicitly considers the intra-pore levels of turbulent kinetic energy. Transport equations are written in their time-and-volume-averaged form and a volume-based statistical turbulence model is applied to simulate turbulence generation due to the porous matrix. The rate of fuel consumption is described by an Arrhenius expression involving the product of the fuel and oxidant mass fractions. These mass fractions are double decomposed in time and space and, after applying simultaneous time-and-volume integration operations to them, distinct terms arise, which are here associated with the mechanisms of dispersion and turbulence. Modeling of these extra terms remains an open question and the derivations herein might motivate further development of models for turbulent combustion in porous media.     

Robust ℋ︁∞ filtering for uncertain Markovian jump linear systems

This paper investigates the problem of ??_∞ filtering for a class of uncertain Markovian jump linear systems. The uncertainty is assumed to be norm‐bounded and appears in all the matrices of the system state‐space model, including the coefficient matrices of the noise signals. It is also assumed that the jumping parameter is available. We develop a methodology for designing a Markovian jump linear filter that ensures a prescribed bound on the ??₂‐induced gain from the noise signals to the estimation error, irrespective of the uncertainty. The proposed design is given in terms of linear matrix inequalities. Copyright © 2002 John Wiley & Sons, Ltd.     

Reliable indirect competitive ELISA used for a survey of ochratoxin A in green coffee from the North of Paraná State, Brazil.

The performance of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) for ochratoxin A (OTA) detection was evaluated in a comparative study with high-performance liquid chromatography (HPLC) analysis using 68 freshly harvested coffee samples from the North of Paraná State, Brazil. The anti-OTA mAb showed high specificity and low cross-reactivity with OTA analogues (OTB and OTalpha), but cross-reacted with OTC. This ic-ELISA showed a detection limit of 3.75 ngg-1 sample, when compared to 0.80 ngg-1 by HPLC, with an ic-ELISA/HPLC correlation coefficient of 0.90. As regards OTA analysis of these coffee samples, natural contamination was detected in 10 samples (14.7%) by both methods, where the ic-ELISA values (range 3.9-7.3 ngg-1) were 1.1 to 1.6-fold higher than HPLC data (2.7-4.7 ngg-1). Five samples (7.4%) were OTA positive (range 0.84-1.30 ngg-1) only by HPLC assay, probably due to the higher detection limit reached by ic-ELISA. OTA was undetectable in 53 samples (77.9%) by both methods, while all positive samples (range 0.84-7.30 ngg-1) showed OTA levels lower than 8 ngg-1 (maximum limit recommended by the European Union). The matrix interference of green coffee was minimized by dilution of sample extracts before carrying out the ELISA assay. This mAb-based ic-ELISA can be effectively applied for OTA screening in coffee, because it is simple, sensitive and sample preparation is easy.     

Conformational properties of poly(diallyldimethyl ammonium chloride) (PDDA) determined by combination of molecular dynamics, rotational isomeric states and Monte Carlo procedures

A theoretical model describing the conformational properties of poly(diallyldimethyl ammonium chloride) (PDDA) is developed with the following strategy: molecular dynamics simulations are performed on model molecules representing dimers of PDDA. The results are employed to formulate a rotational isomeric state model for these molecules in terms of short-range interactions. Furthermore, the MD trajectories permit the evaluation of conformational energies and probabilities for the 27 conformations allowed to the three bonds sequence contained in the dimers. These probabilities are then employed to generate single chains of PDDA in vacuo according to standard Monte Carlo procedures and their main squared end to end distance 〈r²〉 are computed. The procedure (MD simulations, evaluation of energies and probabilities and MC calculations) is then repeated for two more realistic systems obtained by fitting into a cubic box, having side length L and periodic boundary conditions, a forty repeating units oligomer of PDDA together with 40 Cl⁻ as counterions and 500 water molecules (referred to as water system, ) or the oligomer with its counterions, 500 water molecules and 20 NaCl molecules (referred to as salt system, ). The behavior of the three systems is noticeably different and the value of unperturbed dimensions computed for the realistic systems are in good agreement with preliminary results of experimental measurements being carried out in our laboratory.     

Evaluation of indoor gamma radiation dose in dwellings

The use of materials containing naturally occurring radionuclides for house construction may enhance the natural radiation background to which some population groups are exposed. External exposure results from gamma emitter radionuclides existing in the walls, floor and ceiling. Mathematical models can be used to predict external dose rates inside a room, provided the compartment geometry and the radionuclide concentration activities are known. This paper presents a methodology and a computer code for theoretical evaluation of indoor external gamma doses in the air. The room was modelled as three pairs of rectangular slabs of finite thickness. Doses were evaluated by applying a photon transport model, taking into account self-absorption and radiation build-up. Calculations were performed for 40K, 226Ra and 232Th, considering concrete walls. The results obtained show good agreement with those reported in the literature. Dose conversion factors are presented in a practical manner, ready to use for radiological impact screening.