Measurement of secondary colony formation after 5 weeks in long-term cultures in patients with myelodysplastic syndrome

Pancytopenia is a frequent manifestation of myelodysplastic syndromes (MDS). In the presence of an empty bone marrow, clinical distinction from aplastic anemia may be difficult. The hypoplastic marrow morphology seen in some cases of MDS raises questions about etiologic and pathophysiologic relationships between aplastic anemia and MDS. The goal of our study was to compare the degree of the hematopoietic failure in these diseases at the level of the most immature progenitor and stem cells that can be measured in vitro. In a systemic, prospective fashion, we have studied bone marrow (n = 45) and peripheral blood (n = 33) of patients with MDS for the number of long-term culture initiating cells (LTC-IC) in comparison to 17 normal controls and patients with new, untreated aplastic anemia (46 marrow; 62 blood samples). Due to the low numbers of cells available for the analysis, formal limiting dilution analysis could not be performed, instead secondary colony-forming cells (CFC) after 5 weeks of LTBMC were measured. As the number of these cells is proportional to the input number of LTC-IC, the number of secondary CFC per 10(6) mononuclear cells (MNC) initiating the LTBMC can be used as a measure of the content of immature stem cells in bone marrow and peripheral blood. The MDS group consisted of 34 RA, three RARS, eight RAEB and two RAEB-T patients with mean absolute neutrophil values of 1992, 1413, 1441, and 380 per mm3, respectively. The diagnosis was established based on bone marrow morphology and results of cytogenetic studies. In comparison to controls (147 +/- 38/10(6) MNC), significantly decreased numbers of bone marrow secondary CFC were found in MDS: in patients with RA and RARS, 21 +/- 7 secondary CFC per 10(6) bone marrow MNC (P < 0.001); patients with RAEB and RAEB-T: 39 +/- 12 CFC per 10(6) marrow MNC (P < 0.001). In all groups tested, the decrease in peripheral blood secondary CFC numbers was consistently less pronounced. In MDS patients with hypocellular bone marrow, secondary CFC were lower but not significantly different in comparison to MDS with hypercellular marrow (18 +/- 6 vs 35 +/- 11; NS; hypoplastic bone marrow also was not associated with significantly lower neutrophil counts). However, in 24% of patients with MDS, bone marrow secondary CFC were within the normal range, while in the aplastic anemia group only one of the patients showed secondary CFC number within normal range. Bone marrow and blood secondary CFC numbers in hypoplastic RA were significantly higher than those in severe aplastic anemia 919 +/- 5 in bone marrow, P < 0.01; 7 +/- 2 in blood, P < 0.05). This trend was even more pronounced in hypoplastic RA with chromosomal abnormalities. However, no significant differences were found between the secondary CFC numbers in hypoplastic RA and moderate aplastic anemia. We concluded that, although the deficiency in the stem cell compartment is less severe in MDS than in aplastic anemia, depletion of early hematopoietic cells is an essential part of the pathophysiology in both diseases.     

Repeatable generalized inverse control strategies for kinematicallyredundant manipulators

The issue of generating a repeatable control strategy which possesses the desirable physical properties of a particular generalized inverse is addressed. The technique described is fully general and only requires a knowledge of the associated mill space of the desired inverse. While an analytical representation of the null vector is desirable, ultimately the calculations are done numerically so that a numerical knowledge of the associated full vector is sufficient. This method first characterizes repeatable strategies using a set of orthonormal basis functions to describe the null space of these transformations. The optimal repeatable inverse is then obtained by projecting the null space of the desired generalized inverse onto each of these basis functions. The resulting inverse is guaranteed to be the closest repeatable inverse to the desired inverse, in an integral norm sense, from the set of all inverses spanned by the selected basis functions. This technique is illustrated for a planar, three-degree-of-freedom manipulator and a seven-degree-of-freedom spatial manipulator     

Novel phthalocyanine/polyol high-solids coatings: Structure—property relationships

A convenient synthetic procedure for preparing nickel carboxylated phthalocyanine is reported. Upon further hydroxylation, such metal phthalocyanine moieties are incorporated into several melamine-based polyester high-solids systems and the structure—property correlations are examined. Molecular level spectroscopic FTIR analysis and mechanical testing are correlated in an effort to establish structure—property relations in these coatings. It is found that the presence of metal phthalocyanine macrocycle as a cross-linking agent enhances such properties as adhesion, hardness, and impact resistance. © 1993 John Wiley & Sons, Inc.     

Gas recycling as a means of controlling the operation of cyclones

A method is proposed for increasing the dedusting efficiency of cyclones. The method, which is based on the introduction of the gas undergoing purification into a recycle loop, is of considerable practical importance as it enables the process to be controlled so as to maintain a constant efficiency of dust removal.     

Sealed NiCd cells: The temperature behaviour of electrodes in excess of electrolyte

The temperature behaviour of both cadmium and nickel oxide electrodes of the sintered type (as used in sealed NiCd cells) has been investigated     

Numerical limit analysis of perforated plates

Full limit analysis is preseted of a thin plate under plane stress, perforated with circular holes arranged in a regular penetration pattern. The material is assumed to be elastic–perfectly plastic and to obey the Huber–Mises yield condition together with its associated flow law. The finite element tangential stiffness method is employed. Triangular constant-strain elements are used, the load being generated by means of constant edge displacements at the periphery of a suitably selected subregion. Load-displacement diagrams are obtained for various edge displacement programs and then an interaction curve is constructed for the perforated plate considered. Each computational step is associated with a certain plastic zone which develops until the limit state configuration is reached. The plate is provided with equilateral triangular configuration of holes with arbitrary cutout coefficients. The interaction curves are plotted against the Huber–Mises yield condition for the plate without openings.