Unprecedented selectivity to the direct desulfurization (DDS) pathway in a highly active FeNi bimetallic phosphide catalyst

The hydrodesulfurization (HDS) of the refractory compound 4,6-dimethyldibenzothiophene (DMDBT) normally proceeds through a hydrogenation pathway that removes the planarity of the ring system and makes the hindered sulfur atom more accessible to the desulfurization centers. In this study, a highly active dispersed bimetallic NiFeP catalyst is found to have high selectivity for a direct desulfurization pathway, which does not require prior hydrogenation. The compound has equal numbers of Ni and Fe atoms which extended X-ray absorption fine structure analysis indicates are distributed randomly in the hexagonal Fe₂P structure, with just a slight enrichment of Fe on the surface. This is supported by density functional theory calculations. The remarkable properties of the catalyst are ascribed to a ligand effect of Fe on the active Ni atoms.     

Are you in top 1% (1‰)?

A new simple bibliometric indicator is based on the number of highly cited papers (as defined by WoS^®). It can be used to assess individuals, journals and universities. Unlike most other citation-based-indicators it equalizes the chances of young scientists (vs. their more experienced colleagues) and of scientists working in less-popular disciplines. The ranking of scientists based on the new indicator is not correlated with the rankings based upon the number of citations or on the Hirsch-index.     

The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids

Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind genetic material (multiplied positive electric charge) while lowering undesirable effects (the presence of lipids makes the system more like natural membranes). This study aimed to test the ability to bind and release genetic material by lipoplexes based on trimeric surfactants and lipid formulations of different compositions and to characterize formed complexes by circular dichroism (CD) spectroscopy and atomic force microscopy (AFM). The cytotoxicity of studied lipoplexes was tested on HeLa cells by the MTT cell viability assay and the dye exclusion test (trypan blue). The presence of lipids in the system lowered the surfactant concentration required for complexation (higher efficiency) and reduced the cytotoxicity of lipoplexes. Surfactant/lipids/DNA complexes were more stable than surfactant/DNA complexes. Surfactant molecules induced the genetic material condensation, but the presence of lipids significantly intensified this process. Systems based on trimeric surfactants and lipid formulations, particularly TRI_N and TRI_IMI systems, could be used as delivery carrier, and have proven to be highly effective, nontoxic, and universal for DNA of various lengths.     

Effect of phosphorylation of the maize starch on thermal generation of stable and short‐living radicals

Effect of hydrolysis, phosphorylation and treatment with high hydrostatic pressure on thermal generation of stable and short‐living radicals in maize starch was studied by X‐ray diffraction (XRD), electron paramagnetic resonance (EPR) spectroscopy, differential scanning calorimetry and polarized light microscopy. Phosphorus was introduced into maize starch as mono‐ and distarch phosphates. XRD indicated localization of phosphate groups in amorphous part of the granule whereas calorimetric data suggested some cross‐linking of the distarch phosphates. Stable and short‐living radicals with unpaired electron localized at carbon atom were generated in all investigated samples in the temperature range commonly used for processing food. The number of detected short‐living radicals, stabilized by a spin trap, is of two orders of magnitude greater than that of the stable radical species. Hydrolysis and phosphorylation strongly increase the number of stable radicals while pretreatment of the starch with high hydrostatic pressure diminishes their amount. The EPR spectra of stable radicals consist of two components, single line and another one with hyperfine structure, indicating interaction of unpaired electron with neighboring hydrogen atom. The EPR spectra of the spin trap adducts with short‐living radicals contain three components from species differing in their dynamic properties depending on localization in zones of various degree of crystallinity.     

Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives

The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin. Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available. Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies. Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia. We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5. A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP. Highly pure biologically active protein has been obtained by an improved two-step purification procedure. Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP. The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology. However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.     

2,3-Dihydro-1H-cyclopenta[b]quinoline Derivatives as Acetylcholinesterase Inhibitors-Synthesis, Radiolabeling and Biodistribution

In the present study we describe the synthesis and biological assessment of new tacrine analogs in the course of inhibition of acetylcholinesterase. The obtained molecules were synthesized in a condensation reaction between activated 6-BOC-hydrazinopyridine-3-carboxylic acid and 8-aminoalkyl derivatives of 2,3-dihydro-1H-cyclopenta[b]quinoline. Activities of the newly synthesized compounds were estimated by means of Ellman's method. Compound 6h (IC(50) = 3.65 nM) was found to be most active. All obtained novel compounds present comparable activity to that of tacrine towards acetylcholinesterase (AChE) and, simultaneously, lower activity towards butyrylcholinesterase (BChE). Apart from 6a, all synthesized compounds are characterized by a higher affinity for AChE and a lower affinity for BChE in comparison with tacrine. Among all obtained molecules, compound 6h presented the highest selectivity towards inhibition of acetylcholinesterase. Molecular modeling showed that all compounds demonstrated a similar binding mode with AChE and interacted with catalytic and peripheral sites of AChE. Also, a biodistribution study of compound 6a radiolabeled with (99m)Tc was performed.     

Usability assessment of selected methods of optimization for some measurement task in coordinate measurement technique

This paper presents the comparison of selected methods of optimization on the example of some measurement task in coordinate measurement technique. The task consists in the computation of coordinates of the center and radius of a circle which approximates some set of measured points using least squares. The application of the least squares method follows an approach used in conventional analysis of an associated element in coordinate measurement technique. The coordinates of points are measured on a CNC machine tool equipped with a measuring probe.     

Biodegradable latexes from animal-derived waste: Biosynthesis and characterization of mcl-PHA accumulated by Ps. citronellolis

Backgroundmcl-PHA biosynthesis by Pseudomonas citronellolis from tallow-based biodiesel as inexpensive carbon feed stock was accomplished. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and a detailed product characterization are presented.ResultsA maximum specific growth rate, μ_max. of 0.10 and 0.08 h^?1, respectively, was achieved in two different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.036 g/L h and 0.050 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA to 20.1 and 26.6 wt.%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. This was confirmed by ¹H- and ¹³C NMR, also evidencing the occurrence of low quantities of unsaturated and 3-hydroxyvalerate building blocks. High purity of the recovered materials was proofed by elemental analysis. Regarding the results from thermogravimetric analysis, differential scanning calorimetry and molecular mass determination, results were in a range typical for this type of PHA (1st fermentation: decomposition temperature T_d = 296 °C, peak of melting range T_m = 48.6 °C; glass transition temperature T_g = ?46.9 °C, degree of crystallinity X_c = 12.3%, M_w = 66,000, M_n = 35,000, dispersity index P_i = 1.9; 2nd fermentation: T_d = 295 °C, T_m = 53.6 °C, T_g = -43.5 °C, X_c = 10.4%, M_w = 78,000, M_n = 196,000, P_i = 2.5).     

A new busy signal-based MAC protocol supporting QoS for ad-hoc networks with hidden nodes

This article presents three versions of a novel MAC protocol for IEEE 802.11 ad-hoc networks called Busy Signal-based Mechanism turned On (BusySiMOn) (This is an extended version of our conference paper: [15]). The key idea of the proposed solution is based on an intelligent two-step reservation procedure combined with the advantages of EDCA service differentiation. The former alleviates the hidden node problem while the latter ensures compatibility with the IEEE 802.11 standard. Simulation results obtained for saturated and non-saturated network conditions emphasize the advantages of the new protocol over the currently used four-way handshake mechanism in terms of fairness, throughput, and average frame delay.