Imaginary refractive-index effects on desert-aerosol extinction versus backscatter relationships at 351 nm: numerical computations and comparison with Raman lidar measurements

A numerical model is used to investigate the dependence at 351 nm of desert-aerosol extinction and backscatter coefficients on particle imaginary refractive index (mi). Three ranges (-0.005 < or = mi < or = -0.001, -0.01 < or = mi < or = -0.001, and -0.02 < or = mi < or = -0.001) are considered, showing that backscatter coefficients are reduced as /mi/ increases, whereas extinction coefficients are weakly dependent on mi. Numerical results are compared with extinction and backscatter coefficients retrieved by elastic Raman lidar measurements performed during Saharan dust storms over the Mediterranean Sea. The comparison indicates that a range of -0.01 to -0.001 can be representative of Saharan dust aerosols and that the nonsphericity of mineral particles must be considered.     

Gated fiber-optic-coupled detector for in vivo real-time radiation dosimetry

Gated detection of the output of a fiber-optic-coupled radiation dosimeter effectively eliminated the direct contribution of Cerenkov radiation to the signal. The radiation source was an external beam radiotherapy machine that provided pulses of 6-MeV x rays. Gated detection was used to discriminate the signal collected during the radiation pulses, including Cerenkov interference, from the signal collected between the radiation pulses due only to phosphorescence from the Cu(1+)-doped glass detector. Gated detection of the long-lived phosphorescence of the Cu(1+)-doped glass provided real-time dose measurements that were linear with the absorbed dose and that were accurate for all field sizes studied.     

Flexible Multibody Systems Models Using Composite Materials Components

The use of a multibody methodology to describe the large motion of complex systems that experience structural deformations enables to represent the complete system motion, the relative kinematics between the components involved, the deformation of the structural members and the inertia coupling between the large rigid body motion and the system elastodynamics. In this work, the flexible multibody dynamics formulations of complex models are extended to include elastic components made of composite materials, which may be laminated and anisotropic. The deformation of any structural member must be elastic and linear, when described in a coordinate frame fixed to one or more material points of its domain, regardless of the complexity of its geometry. To achieve the proposed flexible multibody formulation, a finite element model for each flexible body is used. For the beam composite material elements, the sections properties are found using an asymptotic procedure that involves a two-dimensional finite element analysis of their cross-section. The equations of motion of the flexible multibody system are solved using an augmented Lagrangian formulation and the accelerations and velocities are integrated in time using a multi-step multi-order integration algorithm based on the Gear method.     

Laplace Plane Analysis of Transient Impedance Between Acupuncture Points Li-4 and Li-12

AC impedance between two acupuncture points (Li-4 and Li-12) and two pairs of anatomically similar points with the same separation was studied via Laplace plane analysis of the time domain current response to a predetermined voltage perturbation. The series resistance between the two acupuncture points was found to be significantly lower than between either pair of control points. The characteristic impedance of an equivalent transmission line was lower and the signal propagation velocity higher for the acupuncture point system as compared to both controls.     

Changes in wine yeast storage carbohydrate levels during preadaptation,rehydration and low temperature fermentations

The metabolism of glycogen and trehalose was analysed in a wine yeast strain fermenting at 25 and 13 degrees C. Trehalose and glycogen degradation were completed during the lag phase of fermentation. Ammonia was taken up rapidly and once it had been reduced to negligible amounts, the synthesis of trehalose started. Glycogen followed a similar pattern. If trehalose synthesis was taken as a stress indicator, the fermentation at 13 degrees C could not be considered stressful because the maximum concentrations are similar at both temperatures. In industrial fermentations, and after a preadaptation in grape must for several hours at 18 degrees C, the lag phase was reduced significantly, and this may be why trehalose and glycogen were completely depleted at the beginning of the low temperature fermentation. Various preadaptation conditions were tested so that their influence on trehalose and glycogen degradation could be determined. The presence of fermentable carbon sources, such as glucose or fructose, triggered the mobilisation and use of trehalose. However, just increasing the osmotic pressure did not reduce the trehalose content. No such differences were observed in glycogen metabolism.     

Susceptibility of Listeria monocytogenes isolated from food in Italy to antibiotics

The susceptibility of 148 strains of Listeria monocytogenes isolated from food to antibiotics currently used in veterinary and human therapy was determined by standard agar dilution and disk diffusion methods. The antibiotics included amikacin, amoxicillin, cefazolin, chloramphenicol, erythromycin, flumequine, fosfomycin, gentamicin, kanamycin, lincomycin, oxytetracycline, rifampicin, spiramycin, streptomycin, tetracycline, tobramycin and vancomycin. Soussy's breakpoints and MIC(50)-MIC(90) values were used to classify the strains into sensitive, moderately sensitive and resistant groups.This work is part of a wider surveillance program on listeriosis started in Italy in 1995.     

Immunocytochemical study of alpha 1 and beta 2/3 subunits of GABAA receptors in freehand isolated vestibular Deiters' neurons

Vestibular Deiters' neurons have been isolated from bovine brain by the Hydén's freehand dissection technique and challenged with monoclonal antibodies directed toward the alpha 1 and beta 2/3 subunits of the GABAA receptors. Subsequent challenge with fluorescent secondary antibodies and confocal microscopy allowed the study of the cellular distribution of such subunits. In Deiters' neurons the beta 2/3 subunit displayed a clear presence all along the cell body profile and the initial parts of the dendrites. The alpha 1 subunit was found highly present all over the cell interior except the nuclear profiles. The strong presence inside the cells possibly masked its presence on the plasma membrane. However, in part of the cells studied a distinct presence on the plasma membrane was evident. This subunit was visualized also all along the long dendrites of these neurons. The approach we describe here, involving freehand isolated mature neurons from adult animals, may allow a better characterization of the tridimensional distribution of different types of neuronal GABAA receptors in the respect of the approach with brain slices.     

Skin nanostructural features determine suture biomechanics

The stress state surrounding wounds in the skin plays an important role in the healing process; it affects the tissue strength, its aesthetic, and its resistance to infections. In this paper, the collagen fibril and elastin matrix damage mechanics following suture point application is investigated at the nanoscale; to this purpose, a model has been developed, which accounts for the architectural and mechanical features of the tissue components. Results indicate that the force displacement caused by the suture point application curve initially stiffens and subsequently softens. Softening occurs due at first to the enlargement of the elastin matrix damaged area and second to the collagen fibril disruption. Three regions may be identified: the first one, (0-0.38 N) refers to skin withstanding the force both in the collagen and elastin components; the second one (0.38-0.75 N) concerns the mechanism of elastin matrix damage; in the last one (>0.75 N), the collagen fibrils also fail. Accordingly, by properly choosing the number of suture points, it is possible to define the optimal suture points number for a given wound closure force.     

Low-density incomplete <Emphasis Type="Italic">LDL</Emphasis><Superscript><Emphasis Type="Italic">T</Emphasis></Superscript> factorizations

A particular class of incomplete factorizations is proposed as preconditioners for the linear system Ax = b where A is a symmetric, large and sparse matrix. The ILDL ^T< (p) factorization (p = 1,2,3, …) determines the density of the lower triangular matrix L selecting the p largest off-diagonal entries of each column during the Gaussian elimination process. This selection may be computationally expensive, but the effectiveness of the preconditioner allows us to choose very low-density factors to reduce both work time and storage requirements. This incomplete factorization can be performed reliably on H-matrices. When A is a positive definite matrix, but not an H-matrix, one can perform an incomplete factorization if positive off-diagonal entries are removed or reduced and diagonally compensated. Numerical results for a variety of problems and comparisons with other incomplete factorizations are presented. Received: August 2002 / Accepted: December 2002 RID="*" ID="*"This work was supported by the Spanish grant BFM 2001-2641.