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11.
Mariangela Lombardi Valentina Naglieri Jean-Marc Tulliani Laura Montanaro 《Journal of Porous Materials》2009,16(4):393-400
An improved gel-casting procedure was successfully exploited to produce porous ceramic bodies having controlled porosity features
in terms of mean pore size, total pore volume as well as pore geometry. The gel-casting process in which a natural gelatine
for food industry is used as gelling agent was firstly set-up to prepare dense alumina and zirconia components. Then, commercial
PE spheres, sieved to select proper dimensional ranges, were added to the starting slurries as pore-forming agent. Both alumina
and zirconia porous bodies were then produced, having a porosity ranging between 40 and 50 vol%. The fired components were
characterised by spherical pores surrounded by highly dense ceramic walls and struts, having a homogeneous and fine microstructure.
Their mean pore size was directly dependent on the sieved fraction of the starting PE spheres selected as pore-forming phase. 相似文献
12.
Carletti P Masi A Spolaore B Polverino De Laureto P De Zorzi M Turetta L Ferretti M Nardi S 《Journal of chemical ecology》2008,34(6):804-818
Humic substances are known to affect plant metabolism at different levels. We characterized humic substances extracted from earthworm feces by diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy and used them to treat corn, Zea mays L., seedlings to investigate changes in patterns of root protein expression. After root plasma membrane extraction and purification, proteins were separated by two-dimensional gel electrophoresis, and differential spot intensities were evaluated by image analysis. Finally, 42 differentially expressed proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The majority of them were downregulated by the treatment with humic substances. The proteins identified included malate dehydrogenase, ATPases, cytoskeleton proteins, and different enzymes belonging to the glycolytic/gluconeogenic pathways and sucrose metabolism. The identification of factors involved in plant responses to humic substances may improve our understanding of plant-soil cross-talk, and enable a better management of soil resources. 相似文献
13.
Vanessa Ribeiro Pestana Rui Carlos Zambiazi Carla R. B. Mendonça Mariangela H. Bruscatto María Jesús Lerma-García Guillermo Ramis-Ramos 《Journal of the American Oil Chemists' Society》2008,85(11):1013-1019
The quality changes and the concentrations of tocopherols and γ-oryzanol, during successive steps of rice bran oil refining
(RBO), were studied. For this purpose, samples of crude, degummed, neutralized, bleached, dewaxed and deodorized RBO were
taken from an industrial plant and analyzed. The moisture, pH, acidity, peroxide value and unsaponifiable matter, were determined.
The fatty acid composition was evaluated by GC, and the concentrations of tocopherols and γ-oryzanol were determined using
HPLC with fluorescence and UV–Vis detection, respectively. To identify γ-oryzanol components, fractions of the HPLC eluant
were collected and analyzed using mass spectrometry. Oil refining reduced the peroxide value and acidity to 1 and 3% of the
values obtained in crude RBO, respectively. The fatty acid composition were not significantly altered during refining. The
concentrations of the tocopherols in RBO followed the order α > (β + γ) > δ. The total concentration of tocopherols was 26 mg/100 g,
and remained practically unaltered during refining. Up to nine components were distinguished in γ-oryzanol. After collecting
the elution fractions, up to six components were identified by electrospray mass spectrometry. Refining reduced the total
concentration of γ-oryzanol to 2% of its initial value. 相似文献
14.
Alessandra Bianco Ilaria Cacciotti Mariangela Lombardi Laura Montanaro Edoardo Bemporad Marco Sebastiani 《Ceramics International》2010,36(1):313-322
Fluorine-substituted hydroxyapatites are considered promising materials for bone scaffolding. In this study a systematic investigation on F-half substituted hydroxyapatite (F-HAp, Ca10(PO4)6OHF) obtained by precipitation is reported. Results on composition analysis, thermal behaviour, and sinterability are presented for a comparison with the respective pure hydroxyapatite. Samples were characterised by electron microscopy, induced coupled plasma-atomic emission spectroscopy, thermal analysis, infrared spectroscopy, N2 adsorption measurements, X-ray diffraction and dilatometry. A semicrystalline F-substituted hydroxyapatite powder made up of needle-like nanoparticles was obtained. Notwithstanding that it was thermally stable up to 1300 °C, it was sintered at relatively low temperatures obtaining a very fine microstructure with a relevant nano-porosity, beneficial in view of cell adhesion and in-growth.Hardness, elastic modulus and toughness of produced samples were investigated by Vickers–Knoop microhardness testing. Significant improvements of the mechanical properties were observed for the F-substituted hydroxyapatite, in comparison with pure hydroxyapatite. 相似文献
15.
Antonio Bevilacqua Mariangela Gallo Maria Rosaria Corbo Milena Sinigaglia 《International Journal of Food Science & Technology》2013,48(7):1366-1370
The main goal of this research was the evaluation of the survival of Enterobacter cloacae in a model olive brine. Two different assays were run; the first experiment assessed the viability of the target in brines containing NaCl (6–12%) and p‐coumaric acid (0.0–0.05%), adjusted to different pHs (4–10) and stored at 10–30 °C for 9 days. The death rate and cell levels at selected times were modelled with a polynomial equation to highlight the individual and interactive effects of NaCl/p‐coumaric acid/pH/temperature. Then, a second experiment was run for 3 months (temperature, 10 °C; pH, 4.5–5.5; NaCl, 6–8%). The survival of E. cloacae was affected mainly by pH, then by salt and temperature; however, the significance of the variables changed within the time, as salt and temperature acted in a significant way only after 1 day. 相似文献
16.
Marcella Mastromatteo Alessandra Danza Mariangela Guida Matteo Alessandro Del Nobile 《International Journal of Food Science & Technology》2012,47(10):2109-2116
In this work, the hydration process of durum wheat–based functional bread loaded with yellow pepper flour was optimised. In particular, the investigated vegetable flour and durum wheat semolina were mixed after they were separately hydrated. Three different amounts of water added to the yellow pepper flour were studied for assessing the effect of vegetable flour hydration level on the dough development and overall quality of bread. The bread formulation investigated in a previous work, based on 25% of yellow pepper and 2% of guar seed as structuring agent where the vegetable flour was directly added to the hydrated durum wheat semolina dough, was chosen as control sample. Results highlighted that dough samples with yellow pepper flour hydrated at highest water content showed a rheological behaviour similar to the durum wheat dough. Moreover, creep analysis showed that the sample added with no‐hydrated yellow pepper flour recorded the greatest resistance to deformation. Same results were obtained for the dough tensile and bread compression tests. The use of the hydrated yellow pepper flour also improved all sensorial attributes. 相似文献
17.
Antonio Bevilacqua Marianna Cannarsi Mariangela Gallo Milena Sinigaglia Maria Rosaria Corbo 《Journal of food science》2010,75(1):M53-M60
ABSTRACT: Enterobacter cloacae can be recovered in the spontaneous fermentations of Italian table olives. In this study, the effects of salt (20 to 100 g/L), temperature (10 to 37 °C), pH (4 to 5 and 8 to 10), p -coumaric and vanillic acids (0.5 to 2 g/L), and the acidification of the medium through lactic, citric, and ascorbic acids were investigated on 15 strains of E. cloacae , isolated from Italian table olives "Bella di Cerignola." Finally, a confirmatory experiment in synthetic brine was run. The strains were inhibited only by an NaCl amount of 70 to 80 g/L and by p -coumaric acid; on the other hand, they showed the ability to grow also at low temperatures (10 to 15 °C). The confirmatory experiment highlighted their ability to survive both at 15 °C and at pH 5. Enterobacter cloacae could be a real problem for the fermentation of table olives in southern Italy; some hurdles could be used (salt or brine acidification), but some environmental conditions (for example, the temperature) should be controlled carefully to maintain olive safety at acceptable levels. 相似文献
18.
Adele H. Marshall Mariangela Zenga 《International Transactions in Operational Research》2009,16(2):213-226
Coxian phase-type distributions are a special type of Markov model that can be used to represent survival times in terms of phases through which an individual may progress until they eventually leave the system completely. Previous research has considered the Coxian phase-type distribution to be ideal in representing patient survival in hospital. However, problems exist in fitting the distributions. This paper investigates the problems that arise with the fitting process by simulating various Coxian phase-type models for the representation of patient survival and examining the estimated parameter values and eigenvalues obtained. The results indicate that numerical methods previously used for fitting the model parameters do not always converge. An alternative technique is therefore considered. All methods are influenced by the choice of initial parameter values. The investigation uses a data set of 1439 elderly patients and models their survival time, the length of time they spend in a UK hospital. 相似文献
19.
Lattice dynamical calculations on trans-planar polyethylene chains containing conformational defects of the type GGTGG have been carried out. The purpose of the work is to predict the points in the vibrational infra-red and/or Raman spectrum at which a tight, (200) type, fold should show absorptions and/or scattering. Calculations are verified for the cyclic hydrocarbon C34H68 which contains two GGTGG defects. The usefulness and limitations of the vibrational spectrum as evidence for the existence of (200) tight folds are discussed. Two infra-red bands near 1342 cm?1 and a third near 700 cm?1 may indicate GGTGG defects in single crystals. Raman spectra seem insensitive to such defects. 相似文献
20.
Mariangela Scalise Fabiola Marino Luca Salerno Eleonora Cianflone Claudia Molinaro Nadia Salerno Antonella De Angelis Giuseppe Viglietto Konrad Urbanek Daniele Torella 《International journal of molecular sciences》2021,22(24)
Organoids are tiny, self-organized, three-dimensional tissue cultures that are derived from the differentiation of stem cells. The growing interest in the use of organoids arises from their ability to mimic the biology and physiology of specific tissue structures in vitro. Organoids indeed represent promising systems for the in vitro modeling of tissue morphogenesis and organogenesis, regenerative medicine and tissue engineering, drug therapy testing, toxicology screening, and disease modeling. Although 2D cell cultures have been used for more than 50 years, even for their simplicity and low-cost maintenance, recent years have witnessed a steep rise in the availability of organoid model systems. Exploiting the ability of cells to re-aggregate and reconstruct the original architecture of an organ makes it possible to overcome many limitations of 2D cell culture systems. In vitro replication of the cellular micro-environment of a specific tissue leads to reproducing the molecular, biochemical, and biomechanical mechanisms that directly influence cell behavior and fate within that specific tissue. Lineage-specific self-organizing organoids have now been generated for many organs. Currently, growing cardiac organoid (cardioids) from pluripotent stem cells and cardiac stem/progenitor cells remains an open challenge due to the complexity of the spreading, differentiation, and migration of cardiac muscle and vascular layers. Here, we summarize the evolution of biological model systems from the generation of 2D spheroids to 3D organoids by focusing on the generation of cardioids based on the currently available laboratory technologies and outline their high potential for cardiovascular research. 相似文献