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排序方式: 共有725条查询结果,搜索用时 15 毫秒
61.
Marianne Caldow Richard J. Fussell Frankie Smith Matthew Sharman 《Food Additives & Contaminants》2007,24(3):280-284
The EU maximum residue limit (MRL) definition for amitraz is 'the sum of amitraz plus all its metabolites containing the 2,4-aniline moiety, expressed as amitraz'. A rapid and sensitive method has been developed and validated in-house for the determination of total amitraz in pears, strawberries, oranges and honey. Samples were hydrolysed (under acidic followed by alkaline conditions) to convert amitraz to 2,4-dimethyaniline, which was then partitioned into 2,2,4-trimethylpentane prior to quantification by gas chromatography-mass spectrometry. The method was validated at 0.02 and 0.1 mg kg-1 amitraz (well below MRL requirements) with a lowest calibrated level (LCL) for 2,4-dimethylaniline of 0.002 mg kg-1 (equivalent to 0.0024 mg kg-1 amitraz). A single partition step yielded recoveries of ∼60% (with % CV values in the range 3.3-8.2), which is satisfactory for screening purposes. A second partition increased recoveries by 10-20%, making the method suitable for the quantification of residues. 相似文献
62.
Nicole F. Steinmetz Ariane Bize Kim C. Findlay George P. Lomonossoff Marianne Manchester David J. Evans David Prangishvili 《Advanced functional materials》2008,18(21):3478-3486
Nanotechnology seeks to mimic what nature has achieved: self‐assembly at the nanometer scale. Viral nanoparticles (VNPs) provide natural examples of self‐assembled architectures with unique structural and chemical properties. Here, the utilization of an archaeal virus, Sulfolobus islandicus rod‐shaped virus 2 (SIRV2), as a template for site‐selective and spatially controlled bioconjugation is described. SIRV2 is a virus of a hyperthermophilic and acidophilic host, the archaeon S. islandicus growing optimally at 80 °C and pH 3, and is thus, by its nature, an extremely stable VNP. The stability of SIRV2 in different solvent/water mixtures is monitored, and it is found that in other, non‐natural harsh conditions the VNPs also remained intact. Further, the question of whether the particles offer attachment sites allowing for selective chemical modification and decoration with functional ligands using biotin as a probe is addressed. It is found that carboxylate‐, carbohydrate‐ and amine‐selective chemistries are applicable and various biotinylated SIRV2 formulations can be fabricated. Depending on the chemistry and hence attachment site used, the display of the biotin labels can be spatially controlled at the virus body and at the ends. Labeling studies also provide novel insights into the structural properties of SIRV2, indicating that the major coat protein (CP) forms the virus body while the minor CP is located in the tail fibers at the end of the particles. Overall, SIRV2 represents an extremely stable and structurally interesting VNP with the potential for novel nanobiotechnological applications. 相似文献
63.
Ricardo J. F. Branco Dr. Marianne Graber Prof. Dr. Vinciane Denis Jürgen Pleiss Prof. Dr. 《Chembiochem : a European journal of chemical biology》2009,10(18):2913-2919
Hydration is a major determinant of activity and selectivity of enzymes in organic solvents or in gas phase. The molecular mechanism of the hydration of Candida antarctica lipase B (CALB) and its dependence on the thermodynamic activity of water (aw) was studied by molecular dynamics simulations and compared to experimentally determined water sorption isotherms. Hydration occurred in two phases. At low water activity, single water molecules bound to specific water binding sites at the protein surface. As the water activity increased, water networks gradually developed. The number of protein‐bound water molecules increased linearly with aw, until at aw=0.5 a spanning water network was formed consisting of 311 water molecules, which covered the hydrophilic surface of CALB, with the exception of the hydrophobic substrate‐binding site. At higher water activity, the thickness of the hydration shell increased up to 10 Å close to aw=1. Above a limit of 1600 protein‐bound water molecules the hydration shell becomes unstable and the formation of pure water droplets occurs in these oversaturated simulation conditions. While the structure and the overall flexibility of CALB was independent of the hydration state, the flexibility of individual loops was sensitive to hydration: some loops, such as those part of the substrate‐binding site, became more flexible, while other parts of the protein became more rigid upon hydration. However, the molecular mechanism of how flexibility is related to activity and selectivity is still elusive. 相似文献
64.
Dry-pasteurization of egg albumen powder in a fluidized bed. II. Effect on functional properties: gelation and foaming 总被引:1,自引:0,他引:1
Marianne Hammershøj Hans C. Rasmussen Jan H. Carstens & Henrik Pedersen 《International Journal of Food Science & Technology》2006,41(3):263-274
Dry‐pasteurization of egg albumen (EA) powder in a fluidized bed was studied in two experiments. Experiment 1 consisted of two different temperatures (90 and 130 °C) and experiment 2 of two air moisture levels (high and low). Powders were processed from 10 min to 3 h. The reference treatment was traditional pasteurization at 90 °C for 21 h. The fluidized bed treatment effects on gel texture, water‐holding capacity (WHC), protein binding, gel colour, foaming capacity, foam stability and surface pressure were evaluated. Gels of EA powders treated at high temperature and high air moisture levels exhibited higher stress, strain and WHC values than gels of untreated EA powder. The gel colour became significantly darker, greener and less yellow by fluidized bed treatment. Foaming and surface activity properties were un‐affected by the treatments, however, foam stability against liquid drainage correlated positively with surface pressure. 相似文献
65.
In this paper, we consider data analysis methods for knowledge extraction from large water data-sets. More specifically, we try to connect physico-chemical parameters and the characteristics of taxons living in sample sites. Among these data analysis methods, we consider formal concept analysis (FCA), which is a recognized tool for classification and rule discovery on object–attribute data. Relational concept analysis (RCA) relies on FCA and deals with sets of object–attribute data provided with relations. RCA produces more informative results but at the expense of an increase in complexity. Besides, in numerous applications of FCA, the partially ordered set of concepts introducing attributes or objects (AOC poset, for Attribute–Object–Concept poset) is used rather than the concept lattice in order to reduce combinatorial problems. AOC posets are much smaller and easier to compute than concept lattices and still contain the information needed to rebuild the initial data. This paper introduces a variant of the RCA process based on AOC posets rather than concept lattices. This approach is compared with RCA based on iceberg lattices. Experiments are performed with various scaling operators, and a specific operator is introduced to deal with noisy data. We show that using AOC poset on water data-sets provides a reasonable concept number and allows us to extract meaningful implication rules (association rules whose confidence is 1), whose semantics depends on the chosen scaling operator. 相似文献
66.
Soumyadeb Mitra Marianne Winslett Windsor W. Hsu Kevin Chen-Chuan Chang 《The VLDB Journal The International Journal on Very Large Data Bases》2008,17(2):225-242
Intense regulatory focus on secure retention of electronic records has led to a need to ensure that records are trustworthy,
i.e., able to provide irrefutable proof and accurate details of past events. In this paper, we analyze the requirements for
a trustworthy index to support keyword-based search queries. We argue that trustworthy index entries must be durable—the index
must be updated when new documents arrive, and not periodically deleted and rebuilt. To this end, we propose a scheme for
efficiently updating an inverted index, based on judicious merging of the posting lists of terms. Through extensive simulations
and experiments with two real world data sets and workloads, we demonstrate that the scheme achieves online update speed while
maintaining good query performance. We also present and evaluate jump indexes, a novel trustworthy and efficient index for join operations on posting lists for multi-keyword queries. Jump indexes support
insert, lookup and range queries in time logarithmic in the number of indexed documents. 相似文献
67.
68.
Marianne Kjellen 《国际水资源开发杂志》2000,16(1):143-154
In many cities in developing countries, a piped water supply is the norm for richer households, while poorer households struggle with a number of alternative means for accessing water. In Dar es Salaam, as the public water supply is highly deficient, households of all income classes draw upon a variety of water sources. Distribution by container using pushcarts is common in large areas, in particular where piped water is rationed, low pressure or non-existent. Tankers complement the public supply in wealthier areas. This paper focuses on how public and private systems complement one another with regards to pushcart water distribution. 相似文献
69.
Pollmann Stefan; Maertens Marianne; von Cramon D. Yves; Lepsien Joeran; Hugdahl Kenneth 《Canadian Metallurgical Quarterly》2002,16(1):56
The authors found splenial lesions to be associated with left ear suppression in dichotic listening of consonant-vowel syllables. This was found in both a rapid presentation dichotic monitoring task and a standard dichotic listening task, ruling out attentional limitations in the processing of high stimulus loads as a confounding factor. Moreover, directed attention to the left ear did not improve left ear target detection in the patients, independent of callosal lesion location. The authors' data may indicate that auditory callosal fibers pass through the splenium more posterior than previously thought. However, further studies should investigate whether callosal fibers between primary and secondary auditory cortices, or between higher level multimodal cortices, are vital for the detection of left ear targets in dichotic listening. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
70.
The first years as independent actors in the housing market: Young households in a Swedish municipality 总被引:1,自引:0,他引:1
Marianne Abramsson Urban Fransson Lars-Erik Borgegård 《Journal of Housing and the Built Environment》2004,19(2):145-168
This study deals with young households at the beginning of their housing career. During the 1990s, a tendency of the young to leave the parental home at a later age than during previous decades was discussed. With extended education, rising housing costs and a shortage of rental housing, difficulties in finding an affordable place to live could be expected to have increased. Parental resources are of increasing importance in times of recession. The aim of this study was to follow the first steps in the housing careers of young individuals in a Swedish municipality and to investigate the time of nest-leaving and the choice of tenure for their first and second moves. Individuals aged 16–25 years in the municipality of Gävle, Sweden, were included in the study. Longitudinal data for the years 1985–1995 were used. To capture changes over time, the nest-leaving situations of two cohorts were compared. Results indicate that the individuals in the 1973 cohort leave home at a higher age than do those in the 1968 cohort. A surprisingly large share move into tenant co-operatives. Such moves, as well as moves into owner occupation, occurred mainly within Gävleborg County. Individuals moving to other municipalities outside the county moved to a larger extent into rental housing. 相似文献