Optical CT reconstruction of 3D dose distributions using the ferrous-benzoic-xylenol (FBX) gel dosimeter

In recent years, magnetic-resonance imaging of gelatin doped with the Fricke solution has been applied to the direct measurement of three-dimensional (3D) radiation dose distributions. However, the 3D dose distribution can also be imaged more economically and efficiently using the method of optical absorption computed tomography. This is accomplished by first preparing a gelatin matrix containing a radiochromic dye and mapping the radiation-induced local change in the optical absorption coefficient. Ferrous-Benzoic-Xylenol (FBX) was the dye of choice for this investigation. The complex formed by Fe3+ and xylenol orange exhibits a linear change in optical attenuation (cm-1) with radiation dose in the range between 0 and 1000 cGy, and the local concentration of this complex can be probed using a green laser light (lambda = 543.5 nm). An optical computed tomography (CT) scanner was constructed analogous to a first-generation x-ray CT scanner, using a He-Ne laser, photodiodes, and rotation-translation stages controlled by a personal computer. The optical CT scanner itself can reconstruct attenuation coefficients to a baseline accuracy of < 2% while yielding dose images accurate to within 5% when other uncertainties are taken into account. Optical tomography is complicated by the reflection and refraction of light rays in the phantom materials, producing a blind spot in the transmission profiles which, results in a significant dose artifact in the reconstructed images. In this report we develop corrections used to reduce this artifact and yield accurate dosimetric maps. We also report the chemical reaction kinetics, the dose sensitivity and spatial resolution (< 1 mm3) obtained by optical absorption computed tomography. The article concludes with sample dose distributions produced by "cross-field" 6 MV x-ray beams, including a radiosurgery example.     

Limiting interactions in decentralized control of MIMO systems

Despite the closed-loop performance advantages of centralized multivariable controllers, the great majority of industrial process control applications is still based on decentralized controllers. Because of their single loop structure, decentralized controllers cannot suppress the interactions of the plant, which are only taken into account in the controller tuning phase. Therefore, it would be useful in many cases to delimit in some way the undesirable effects of the coupling between inputs and outputs of the closed loop system. A control algorithm to achieve this goal is developed in the paper. The proposal is applicable even to processes with non-minimum phase transmission zeros (provided their off-diagonal transfer functions have their zeros in the left-half-plane). Based on sliding mode attributes, the proposed scheme can be easily added to a previously designed decentralized control system.     

Second-order sliding mode observer for multiple kinetic rates estimation in bioprocesses

Specific kinetic rates are key variables regarding metabolic activity in bioprocesses. They are non-linear functions of concentrations and operating conditions and therefore of difficult access for process control. In this paper, a multiple kinetic rates observer based on second-order sliding mode ideas is proposed. The main difference with other proposals is that smooth estimates are achieved in finite-time without adding additional dynamics. The resulting estimator is robust against uncertainty in the model of the estimated variables. Experimental results from continuous fermentation of S. cerevisiae are presented, where microbial specific growth rate and net ethanol production rate are estimated.     

Monte Carlo simulations of DNA damage from incorporated cold iodine following photoelectrically induced Auger electron cascades

Radiation-induced damage in nucleosomal DNA from Auger electron cascades due to incorporated cold IUdR has been modelled through Monte Carlo simulations. Probabilities of DNA double strand break (DSB) production following a vacancy in the K, L, M and N shells of iodine are estimated. DSB complexity from the base damage accompanying a break was also estimated. Multiple DSB events were analysed for correlated breaks due to nucleosome periodicity. The probability of an Auger cascade causing at least one DSB strongly depended on the shell in which the initial vacancy was produced. This probability was approximately 0.35 for K and L shells and fell to 0.02 for the N shell. As expected, DSBs were predominantly induced in a nucleosome containing incorporated iodine and were accompanied with extensive base damage. Analysis of multiple DSB events showed that approximately 14% of the DSBs produced following a vacancy in the L1 orbital can be interpreted as correlated with base pair separation attributable to the nucleosome periodicity. The data generated in this work provide a basis for the development of photon-activation therapy using kilovoltage X rays incident upon IUdR sensitised tumours.     

Effects of fibronectin and laminin on structural, mechanical and transport properties of 3D collageneous network

Recent studies, on cells cultured in 3D collagen gels, have shown that, beside from their well known biochemical role, fibronectin (FN) and laminin (LM) affect cell functions via a modification of mechanical and structural properties of matrix due to interaction with collagen molecules. Though biochemical properties of FN and LM have been widely studied, little is known about their role in collagen matrix assembly. The aim of this work was to characterize FN- and LM-based collagen semi-interpenetrating polymer networks (semi-IPNs), in order to understand how these biomacromolecular species can affect collagen network assembly and properties. Morphology, viscoelasticity and diffusivity of collagen gels and FN- and LM-based collagen semi-IPNs were analysed by Confocal Laser Scanning microscopy (CLSM), Environmental Scanning Electron microscopy (ESEM), Transmission Electron microscopy (TEM), Rheometry and Fluorescence Recovery After Photobleaching (FRAP) techniques. It was found that FN and LM were organized in aggregates, interspersed in collagen gel, and in thin fibrils, distributed along collagen fibres. In addition, high FN and LM concentrations affected collagen fibre assembly and structure and induced drastic effects on rheological and transport properties.     

Drawing trees in a streaming model

We pose a new visualization challenge, asking Graph Drawing algorithms to cope with the requirements of Streaming applications. In this model a source produces a graph one edge at a time. When an edge is produced, it is immediately drawn and its placement cannot be altered. The drawing has an image persistence, that controls the lifetime of edges. If the persistence is k, an edge remains in the drawing for the time spent by the source to generate k edges, and then it fades away. In this model we study the area requirement of planar straight-line grid drawings of trees and we assess the output quality of the presented algorithms by computing the competitive ratio with respect to the best known offline algorithms.     

Short-Term,Voluntary Exercise Affects Morpho-Functional Maturation of Adult-Generated Neurons in Rat Hippocampus

Physical exercise is a well-proven neurogenic stimulus, promoting neuronal progenitor proliferation and affecting newborn cell survival. Besides, it has beneficial effects on brain health and cognition. Previously, we found that three days of physical activity in a very precocious period of adult-generated granule cell life is able to antedate the appearance of the first GABAergic synaptic contacts and increase T-type Ca²⁺ channel expression. Considering the role of GABA and Ca²⁺ in fostering neuronal maturation, in this study, we used short-term, voluntary exercise on a running wheel to investigate if it is able to induce long-term morphological and synaptic changes in newborn neurons. Using adult male rats, we found that: (i) three days of voluntary physical exercise can definitively influence the morpho-functional maturation process of newborn granule neurons when applied very early during their development; (ii) a significant percentage of new neurons show more mature morphological characteristics far from the end of exercise protocol; (iii) the long-term morphological effects result in enhanced synaptic plasticity. Present findings demonstrate that the morpho-functional changes induced by exercise on very immature adult-generated neurons are permanent, affecting the neuron maturation and integration in hippocampal circuitry. Our data contribute to underpinning the beneficial potential of physical activity on brain health, also performed for short times.     

Available Lysine Content during Cereal Flake Production

The available lysine contents during industrial production of wheat, rye, barley and oat flakes have been investigated. Obtained results show that hydrothermal treatment (120°C, 60 min, 1·99×10⁵ Pa) of whole cereal grain as well as flaking process (70°C, gap 0·3 mm) did not significantly affect lysine availability. When summary effects in particular processing phases, including thermal treatment (100°C) of oat grains—before fragmentation and hydrothermic treatment—is calculated, the loss of available lysine was significant during production of rye flakes (4·90%) and oat flakes (8·22%). Protein nutritive quality, expressed as a chemical score based on the available lysine contents in relation to NAS/NRC reference pattern was on the average 47·9%, 46·5%, 41·0% and 38·6% for oat, rye, barley and wheat flakes, respectively. © 1997 SCI     

Low-cost CMOS pulse generator for UWB systems

The design of a CMOS ultra-wideband (UWB) pulse generator for impulse radio system transmission in the 3.1-10.6 GHz frequency band is presented. The pulse generator uses an elementary pulse combiner, which appears to be well suited to tune the synthesised pulse. The generator is designed with no inductors or external filter in order to reduce the die area and the production cost. Simulations with a standard 0.13 mum CMOS design kit show a 600 mV_pp pulse that satisfies the FCC regulation mask and a power consumption of 2.64 mW with a 100 MHz pulse repetition frequency.