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81.
Despite the great technical advancement of mass spectrometry, this technique has contributed in a limited way to the discovery and quantitation of specific/precocious markers linked to free radical-mediated diseases. Unsaturated aldehydes generated by free radical-induced lipid peroxidation of polyunsaturated fatty acids, and in particular 4-hydroxy-trans-2 nonenal (HNE), are involved in the onset and progression of many pathologies such as cardiovascular (atherosclerosis, long-term complications of diabetes) and neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, and cerebral ischemia). Most of the biological effects of HNE are attributed to the capacity of HNE to react with the nucleophilic sites of proteins and peptides (other than nucleic acids), to form covalently modified biomolecules that can disrupt important cellular functions and induce mutations. By considering the emerging role of HNE in several human diseases, an unequivocal analytical approach as mass spectrometry to detect/elucidate the structure of protein-HNE adducts in biological matrices is strictly needed not only to understand the reaction mechanism of HNE, but also to gain a deeper insight into the pathological role of HNE. This with the aim to provide intermediate diagnostic biomarkers for human diseases. This review sheds focus on the "state-of-the-art" of mass spectrometric applications in the field of HNE-protein adducts characterization, starting from the fundamental early studies and discussing the different MS-based approaches that can provide detailed information on the mechanistic aspects of HNE-protein interaction. In the last decade, the increases in the accessible mass ranges of modern instruments and advances in ionization methods have made possible a fundamental improvement in the analysis of protein-HNE adducts by mass spectrometry, and in particular by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) tandem mass spectrometry. The recent developments and uses of combined analytical approaches to detect and characterize the type/site of interaction have been highlighted, and several other aspects, including sample preparation methodologies, structure elucidation, and data analysis have also been considered. 相似文献
82.
Camilo Zúñiga Gerard Lligadas Juan Carlos Ronda Marina Galià Virginia Cádiz 《Polymer》2012,53(15):3089-3095
This work concerns the investigation of porous polybenzoxazines based on the non-toxic renewable diphenolic acid. The approach described relies on the in situ generation of foaming agent (CO2) during the thermal curing. For this purpose, the previously synthesized benzoxazine monomer from diphenolic acid was thermally polymerized at different temperatures. As the beginning of decarboxylation is about 200 °C, we selected five foaming temperatures (Tf) ranging from 190 to 230 °C. The influence of the foaming temperature on the cellular structure and its dependency on final properties is discussed. 相似文献
83.
Dr. Vladimir O. Talibov Dr. Edoardo Fabini Edward A. FitzGerald Dr. Daniele Tedesco Daniela Cederfeldt Martin J. Talu Moira M. Rachman Filip Mihalic Dr. Elisabetta Manoni Dr. Marina Naldi Dr. Paola Sanese Dr. Giovanna Forte Dr. Martina Lepore Signorile Prof. Xavier Barril Dr. Cristiano Simone Prof. Manuela Bartolini Dr. Doreen Dobritzsch Dr. Alberto Del Rio Prof. U. Helena Danielson 《Chembiochem : a European journal of chemical biology》2021,22(9):1597-1608
SMYD3 is a multifunctional epigenetic enzyme with lysine methyltransferase activity and various interaction partners. It is implicated in the pathophysiology of cancers but with an unclear mechanism. To discover tool compounds for clarifying its biochemistry and potential as a therapeutic target, a set of drug-like compounds was screened in a biosensor-based competition assay. Diperodon was identified as an allosteric ligand; its R and S enantiomers were isolated, and their affinities to SMYD3 were determined (KD=42 and 84 μM, respectively). Co-crystallization revealed that both enantiomers bind to a previously unidentified allosteric site in the C-terminal protein binding domain, consistent with its weak inhibitory effect. No competition between diperodon and HSP90 (a known SMYD3 interaction partner) was observed although SMYD3–HSP90 binding was confirmed (KD=13 μM). Diperodon clearly represents a novel starting point for the design of tool compounds interacting with a druggable allosteric site, suitable for the exploration of noncatalytic SMYD3 functions and therapeutics with new mechanisms of action. 相似文献
84.
Recently, feature grouping has been proposed as a method for improving retrieval results for logos and web images. This relies on the idea that a group of features matching over a local region in an image is more discriminative than a single feature match. In this paper, we evolve this concept further and apply it to the more challenging task of landmark recognition. We propose a novel combination of dense sampling of SIFT features with interest regions which represent the more salient parts of the image in greater detail. In place of conventional dense sampling used in category recognition that computes features on a regular grid at a number of fixed scales, we allow the sampling density and scale to vary based on the scale of the interest region. We develop new techniques for exploring stronger geometric constraints inside the feature groups and computing the match score. The spatial information is stored efficiently in an inverted index structure. The proposed approach considers part-based matching of interest regions instead of matching entire images using a histogram under bag-of-words. This helps reducing the influence of background clutter and works better under occlusion. Experiments reveal that directing more attention to the salient regions of the image and applying proposed geometric constraints helps in vastly improving recognition rates for reasonable vocabulary sizes. 相似文献
85.
86.
Violeta Ivanova Marina Stefova Trajče Stafilov Borimir Vojnoski Ildiko Bíró Anita Bufa Ferenc Kilár 《Food Analytical Methods》2012,5(6):1427-1434
An analytical method for determination of volatile composition of wines using sample preparation by liquid–liquid extraction and gas chromatography coupled to mass spectrometry for separation and detection has been developed and validated. Extraction of volatile compounds was performed in dichloromethane, and 1-octanol was added as an internal standard. Kékfrankos red wine produced in Villány wine region in Hungary was used as a model wine for testing and validation of the method. The developed method allowed satisfactory determination of 33 volatile compounds in the wines. Compounds analyzed include alcohols, esters, lactones, fatty acids, furans, and nitrogen compounds. The calibration curves of the four reference compounds used (2-phenyl ethanol, ethyl nonanoate, butyrolactone, and tyrosol) were linear in all cases with correlation coefficients (R 2) ranging from 0.9951 to 0.9992. The accuracy of the method was checked with a standard addition method (recovery 92.2–103 %), showing good repeatability and reproducibility (RSD?<?10 %). 相似文献
87.
Marina G.B. Aleixo Carla A.B. Sass Rafael M. Leal Tiago M. Dantas Mônica M. Pagani Tatiana C. Pimentel Mônica Q. Freitas Adriano G. Cruz Denise R.P. Azeredo Erick A. Esmerino 《International Journal of Food Science & Technology》2021,56(1):61-68
Social media has experienced rapid growth in recent years and has been subjected of discussions on a wide range of topics including new lifestyles and eating habits, providing a great opportunity to obtain spontaneous consumer information. In this sense, the present work aimed to understand the perception of Twitter® users about themes of veganism and plant-based diets. The social networking data mining methodology was applied to measure the relationship between both terms. The significant differences found were analysed using the global chi-square (χ2) test, and their sources of variation were investigated by the chi-square per cell. The results indicate that the vegan group's posts are more related to the categories of recipes, trends, criticism and negative comments about veganism, being more often citing sources when compared to the other group. The results of the plant-based diet group are more significantly related to the impacts of nutrition, physical activity and consumer health. In conclusion, Twitter® has proved to be an interesting tool for obtaining data on (re) produced food publications on social media and their results can guide the market and the academic environment in creating new products, services and marketing strategies to answer the needs of specific consumers. 相似文献
88.
The drive shaft in the propulsion system of a boat broke, while the vessel was sailing along the Western Canadian coast. This
part was made from a low-alloy steel grade 4340 quenched and tempered. Fractographic investigation at macro scale revealed
that the shaft failed under low rotating-bending variable stress. Fatigue propagation occurred on about 95% of the total cross
section of the shaft, under both low-cycle and high-cycle fatigue mechanisms. It was found that the fillet radius present
at the fracture’s origin was smaller than the one provisioned by design. This situation has raised the stress level at this
location higher than the one taken into consideration at the moment of the design calculations and caused the initiation of
the cracking. Moreover, although the shaft has been quenched and tempered, its actual hardness did not have the optimal value
for long-life fatigue strength. 相似文献
89.
Yilin Tian Caleb Arata Erin Boedicker David M. Lunderberg Sameer Patel Sumit Sankhyan Kasper Kristensen Pawel K. Misztal Delphine K. Farmer Marina Vance Atila Novoselac William W. Nazaroff Allen H. Goldstein 《Indoor air》2021,31(1):88-98
Inhalation of particulate matter is associated with adverse health outcomes. The fluorescent portion of supermicron particulate matter has been used as a proxy for bioaerosols. The sources and emission rates of fluorescent particles in residential environments are not well-understood. Using an ultraviolet aerodynamic particle sizer (UVAPS), emissions of total and fluorescent supermicron particles from common human activities were investigated during the HOMEChem campaign, a test-house investigation of the chemistry of indoor environments. Human occupancy and activities, including cooking and mopping, were found to be considerable sources of indoor supermicron fluorescent particles, which enhanced the indoor particle concentrations by two orders of magnitude above baseline levels. The estimated total (fluorescent) mass emission rates for the activities tested were in the range of 4-30 (1-11) mg per person meal for cooking and 0.1-4.9 (0.05-4.7) mg/h for occupancy and mopping. Model calculations indicate that, once released, the dominant fate of coarse particles (2.5-10 micrometer in diameter) was deposition onto indoor surfaces, allowing for the possibility of subsequent resuspension and consequent exposures over durations much longer than the ventilation time scale. Indoor coarse particle deposition would also contribute to soiling of indoor surfaces. 相似文献
90.
Vadim I. Polezhaev Marina N. Myakshina Sergey A. Nikitin 《International Journal of Heat and Mass Transfer》2012,55(1-3):156-165
Coupling of two basic mechanisms of gravity-driven convection is named as convective interactions in the paper. Classification of applications of the interaction mechanism with different orientation of input (output) heat flux to the gravity force is suggested. Brief overview and comments are done. For illustration of the classification a number of examples on the basis of experience in technique and technology are presented. Some convective interaction features with change of orientation for a model of differently heated square are shown. Critical value of the angle for bifurcation onset, heat transfer and temperature stratification in dependency of the angle, temperature oscillations onset in dependency of Pr number for inclined square are presented. 相似文献