Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family

We previously identified the ZNF85 (HPF4) KRAB zinc finger gene, a member of the human ZNF91 family. Here, we show that the ZNF85 gene is highly expressed in normal adult testis, in seminomas, and in the NT2/D1 teratocarcinoma cell line. Immunocytochemical localization of a panel of beta-Gal/ZNF85 fusion proteins revealed that ZNF85 contains at least one nuclear localization signal located in the spacer region connecting the KRAB domain with the zinc finger repeats. Bacterially expressed ZNF85 zinc finger domain bound strongly and exclusively to DNA in vitro in a zinc-dependent manner. The KRAB(A) domain of the ZNF85 protein and of several other members of the ZNF91 family exhibited repressing activity when tested in Gal4 fusion protein assays. The repression was significantly enhanced by the addition of the KRAB (B) domain, whereas further addition of other conserved regions had no effect. The ZNF85 KRAB(A) and (B) domains in vitro bound several nuclear proteins that might constitute critical cofactors for repression.     

模拟电路的故障回路和故障网孔的诊断

文中给出了模拟电路多故障诊断的回路法和网孔法,使K故障诊断理论内容完整,在某些情况下,回路诊断法具有一定的优越性。     

Identification of Abundant and Functional dodecaRNAs (doRNAs) Derived from Ribosomal RNA

Using a modified RNA-sequencing (RNA-seq) approach, we discovered a new family of unusually short RNAs mapping to ribosomal RNA 5.8S, which we named dodecaRNAs (doRNAs), according to the number of core nucleotides (12 nt) their members contain. Using a new quantitative detection method that we developed, we confirmed our RNA-seq data and determined that the minimal core doRNA sequence and its 13-nt variant C-doRNA (doRNA with a 5′ Cytosine) are the two most abundant doRNAs, which, together, may outnumber microRNAs. The C-doRNA/doRNA ratio is stable within species but differed between species. doRNA and C-doRNA are mainly cytoplasmic and interact with heterogeneous nuclear ribonucleoproteins (hnRNP) A0, A1 and A2B1, but not Argonaute 2. Reporter gene activity assays suggest that C-doRNA may function as a regulator of Annexin II receptor (AXIIR) expression. doRNAs are differentially expressed in prostate cancer cells/tissues and may control cell migration. These findings suggest that unusually short RNAs may be more abundant and important than previously thought.     

Detecting small pulmonary nodules with spiral ultrashort echo time sequences in 1.5 T MRI

Magnetic Resonance Materials in Physics, Biology and Medicine - This study investigated ultrashort echo time (UTE) sequences in 1.5 T magnetic resonance imaging (MRI) for small lung nodule...     

Luminescence and bandgap studies of high purity MgXZn1−XTe Ternary Alloys

High purity Mg_XZn_1−XTe alloys in the range of 0 < x < 0.45 have been prepared by the Bridgman technique. Wavelength modulated reflectivity measurements have been performed at 300 K. The structure of the spectra near the fundamen-tal edge is similar to the one for ZnTe. The bandgap va-riation versus the composition x is Eg (x) = 2.271 + 0.7 x + 0.6 x². Cathodoluminescence at 300K and 110K shows a single bandedge narrow peak. The variation with composition of the emission peak energy Ep (X) follows the same law as Eg (x). Undoped crystals are highly resistive (> 10⁵Ωcm) but when phosporus is introduced into the melt, the resis-tivity decreases to 10³ Ωcm.     

济阳拗陷桩海地区下古生界深埋藏溶蚀特征及储集意义

 通过桩海地区下古生界的岩心描述和镜下观察，探讨了埋藏溶蚀作用的特征，建立了埋藏溶蚀作用的识别标志。碳酸盐岩溶洞中有机和无机气液两相包体共生普遍，并与全烃包体伴生，发黄绿色荧光。包裹体均一温度主要为90℃～150℃，具有“双峰”分布特征。δ13C值为-2.2～-3.5； δ18O值为-17.4～-13.2，与加里东期裂缝和印支期裂缝充填物的同位素值相比，埋藏期溶洞充填物碳同位素值具有明显向高值迁移、氧同位素具有向低值迁移的特征。包裹体均一温度和稳定同位素数据支持埋藏溶蚀作用的存在。文中还对研究区桩古10井下古生界储集空间发育特征进行了分析，认为桩古10井残留的马家沟组灰岩受后期埋藏溶蚀作用显著，各种次生的溶蚀洞、扩容缝和溶孔为主要储集空间，储集空间类型为复合型。复合型孔隙的形成主要是孤北洼陷巨厚的第三系沉积物压释水沿边界断层向高部位的桩西潜山运移，作用于桩古10井区的下古生界，在埋藏条件下产生溶蚀而形成。     

REEXAMINATION OF THE ROLE OF VERTICAL CIRCULATION IN SEDIMENTATION IN THE YELLOW AND EAST CHINA SEAS

1 .　ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｒｅａｒｅｔｈｒｅｅｂｉｇｒｉｖｅｒｓｔｈｅＹｅｌｌｏｗ ,ＹａｎｇｔｚｅａｎｄＰｅａｒｌｒｕｎｎｉｎｇｆｒｏｍｔｈｅｃｏｎｔｉｎｅｎｔｏｆＣｈｉｎａｔｏＣｈｉｎａＳｅａｓ.ＴｈｅＹｅｌｌｏｗＲｉｖｅｒｅｍｐｔｉｅｓｓｅｄｉｍｅｎｔｔｏｔｈｅＢｏｈａｉＳｅａｗｉｔｈａｎａｍｏｕｎｔｏｆ 0 .7× 1 0 9ｔｏｎｓｐｅｒｙｅａｒ (Ｈｕｅｔａｌ.1 998ａｎｄＰａｎｇｅｔａｌ.1 999)ｗｉｔｈａｈｉｓｔｏｒｉｃａｎｎｕａｌｍｅａｎｏｆ 1× 1 0 9ｔｏｎｓ (Ｘｉｅｅｔａｌ.1 980 ;Ｍｉｌ…     

A genetic polymorphism of the peroxisome proliferator-activated receptor gamma gene influences plasma leptin levels in obese humans

OBJECTIVE: To determine whether adverse perinatal outcome is associated with asthma or asthma medication use during pregnancy. METHODS: A retrospective cohort study was conducted of women who resided in Halifax County, Nova Scotia, and delivered between 1991 and 1993. Asthmatic women were classified into three groups, according to medication usage: no medications, beta agonists only, and steroids with or without other asthma medications. Outcomes compared among asthmatic and nonasthmatic women included maternal complications (pregnancy-induced hypertension, cesarean delivery, gestational diabetes, preterm birth, and antepartum and postpartum hemorrhage) and neonatal outcomes (low birth weight, congenital malformations, hyperbilirubinemia, and respiratory distress syndrome). RESULTS: The cohort included 817 asthmatic women and 13,709 nonasthmatic women. Overall, the prevalence of pregnancies complicated by asthma increased from 4.8% in 1991 to 6.9% in 1993. Asthmatic women were at increased risk for antepartum and postpartum hemorrhage, independent of medication usage. Asthmatic women taking steroids were at increased risk for pregnancy-induced hypertension (odds ratio [OR] 1.7; 95% confidence interval [CI] 1.0, 2.9). The only significant difference in neonatal outcome between asthma medication groups and nonasthmatic women was of an increased risk of hyperbilirubinemia in infants of women taking steroids (OR 1.9; 95% CI 1.1, 3.4). CONCLUSION: Risk of antepartum and postpartum hemorrhage is increased in asthmatic women, independent of medication usage. The increased incidence of neonatal hyperbilirubinemia and the borderline increased risk of pregnancy-induced hypertension may be complications of steroid use or may be related to poorly controlled asthma.     

Cells and mediators from pharyngeal secretions in infants with acute wheezing episodes

An acute wheezing episode is the most common feature of severe lower respiratory tract infection during infancy. Respiratory syncytial virus (RSV) is the major causative agent. In order to study inflammation during acute wheezing episodes in infants, we wanted to assess the feasibility and contribution of induction of pharyngeal secretions. We therefore compared inflammatory markers in the pharyngeal secretions of 27 infants suffering from acute wheezing episodes with an RSV infection (RSV+) and 18 infants suffering with acute wheezing episodes without RSV infection (RSV-). Pharyngeal secretions were recovered by physiotherapy using isotonic saline. The safety of the procedure was carefully checked. Pharyngeal secretions were homogenized with dithiothreitol. Total cells and eosinophils were counted and levels of eosinophil cationic protein (ECP) and histamine were measured. Induction of pharyngeal secretion was always well tolerated. Eosinophils were present in five RSV+ and seven RSV- patients. ECP levels were not significantly different between the groups. Histamine levels after protein adjustment were significantly increased in RSV+ patients (p<0.01) in comparison to RSV- patients. In this study, we have shown, that pharyngeal secretion can be safely recovered from infants suffering from acute wheezing episodes, and that it can be analysed for enumeration of inflammatory cells and measurement of inflammatory mediators.