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131.
Trichothecenes are secondary metabolites produced by several fungi of the Fusarium genus during their growth period. They inhibit protein biosynthesis in eukaryotic cells resulting in numerous toxic effects such as diarrhea, vomiting, and gastro-intestinal inflammation. Considering its occurrence in food and feedstuff, deoxynivalenol (DON) is one of the most important trichothecenes. We report the synthesis of stable isotope labeled 15-d(1)-deoxynivalenol (15-d(1)-DON) from its natural precursor 3-acetyldeoxynivalenol (3-AcDON) as starting material. Furthermore, a method for the analysis of DON and 3-AcDON using HPLC-MS/MS with stable isotope labeled 15-d(1)-DON and 3-d(3)-AcDON as internal standards has been developed. In total, 18 cereal product samples were analyzed with contamination levels ranging from 10-301 microg/kg for DON and 5-14 microg/kg for 3-AcDON. This is the first report of an isotope dilution MS method for the analysis of type B-trichothecenes. 相似文献
132.
Assessing design features of virtual keyboards for text entry 总被引:1,自引:0,他引:1
OBJECTIVE: The present research examined design of a virtual keyboard for text entry with a rotary controller, emphasizing users who differ in age and system experience. BACKGROUND: Existing research has minimally addressed usage frequency, age, and the effects of display shape and letter arrangement on movement and visual search components of text entry tasks. The present research was conducted to close these gaps. METHOD: Two experiments were completed to examine younger (18-28 years) and older (60-75 years) adults' movement and visual search capabilities using four keyboard shapes and three keyboard arrangements. In a third experiment examining combined effects on shape design, 32 younger (18-28 years) and 32 older (60-75 years) adults entered words on the two best shapes from the first experiments. RESULTS: For the movement task, movement time was lowest for shapes with higher shape-controller compatibility. For the visual search task, search time and accuracy were best on the alphabetic arrangement. In the combined task, shape did not significantly influence performance at different levels of practice. Transfers, however, suggested that the shape with salient visual features elicited a text entry strategy for older adults that may promote more consistent performance under occasional usage. CONCLUSION: The studies together demonstrate that keyboard shape is important for efficient performance. Shape-controller compatibility facilitated performance in both age groups. Salient features facilitate performance, especially for older adults. In nearly all cases alphabetic arrangement yielded the best performance. APPLICATION: Recommendations are provided for virtual keyboard design for different usage frequencies, contexts, and users. 相似文献
133.
Ilkka Niemelä 《Annals of Mathematics and Artificial Intelligence》1999,25(3-4):241-273
Logic programming with the stable model semantics is put forward as a novel constraint programming paradigm. This paradigm
is interesting because it bring advantages of logic programming based knowledge representation techniques to constraint programming
and because implementation methods for the stable model semantics for ground (variable‐free) programs have advanced significantly
in recent years. For a program with variables these methods need a grounding procedure for generating a variable‐free program.
As a practical approach to handling the grounding problem a subclass of logic programs, domain restricted programs, is proposed.
This subclass enables efficient grounding procedures and serves as a basis for integrating built‐in predicates and functions
often needed in applications. It is shown that the novel paradigm embeds classical logical satisfiability and standard (finite
domain) constraint satisfaction problems but seems to provide a more expressive framework from a knowledge representation
point of view. The first steps towards a programming methodology for the new paradigm are taken by presenting solutions to
standard constraint satisfaction problems, combinatorial graph problems and planning problems. An efficient implementation
of the paradigm based on domain restricted programs has been developed. This is an extension of a previous implementation
of the stable model semantics, the Smodels system, and is publicly available. It contains, e.g., built‐in integer arithmetic
integrated to stable model computation. The implementation is described briefly and some test results illustrating the current
level of performance are reported.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献