Involving users in the development of a modeling language for customer journeys

Software and Systems Modeling - Although numerous methods for handling the technical aspects of developing domain-specific modeling languages (DSMLs) have been formalized, user needs and usability...     

Atomization of superheated water: Results from experimental studies

Results from experimental studies of characteristics of atomization of water superheated relative to saturation temperature are presented. The data on disperse composition of a droplet flow are obtained depending on the temperature of water superheat. It is shown that, at a water temperature of 200–240°C, the distribution of droplets as to their size appears to be bimodal, with about 70% of droplets (by mass) having a diameter of not more than 5–6 μm.     

Reduction by gaseous ozone of Salmonella and microbial flora associated with fresh-cut cantaloupe

This research investigates the efficacy of gaseous ozone, applied under partial vacuum in a controlled reaction chamber, for the elimination of Salmonella inoculated on melon rind. The performance of high dose, short duration treatment with gaseous ozone, in this pilot system, on the microbial and sensory quality of fresh-cut cantaloupes was also evaluated. Gaseous ozone (10,000 ppm for 30 min under vacuum) reduced viable, recoverable Salmonella from inoculated physiologically mature non-ripe and ripe melons with a maximum reduction of 4.2 and 2.8 log CFU/rind-disk (12.6 cm(2)), respectively. The efficacy of ozone exposure was influenced by carrier matrix. Salmonella adhering to cantaloupe was more resistant to ozone treatment when suspended in skim-milk powder before aqueous inoculation to the rind. This indicated that organic matter interferes with the contact efficiency and resultant antimicrobial activity of gaseous ozone applied as a surface disinfectant. Conversely, in the absence of an organic carrier, Salmonella viability loss was greater on dry exocarp surfaces than in the wetted surfaces, during ozone treatment, achieving reductions of 2.8 and 1.4 initial log CFU/rind-disk, respectively. Gaseous ozone treatment of 5000 and 20,000 ppm for 30 min reduced total coliforms, Pseudomonas fluorescens, yeast and lactic acid bacteria recovery from fresh-cut cantaloupe. A dose Ct-value (concentration x exposure time) of 600,000 ppm min achieved maximal log CFU/melon-cube reduction, under the test conditions. Finally, fresh-cut cantaloupe treated with gaseous ozone, maintained an acceptable visual quality, aroma and firmness during 7-day storage at 5 degrees C. Conclusions derived from this study illustrate that gaseous ozone is an effective option to risk reduction and spoilage control of fresh and fresh-cut melon. Moreover, depending on the timing of contamination and post-contamination conditions, rapid drying combined with gaseous ozone exposure may be successful as combined or sequential disinfection steps to minimize persistence of Salmonella on the surface of cantaloupe melons and transference during fresh-cut processing of home preparation. Based on these results, greater efficacy would be anticipated with mature but non-ripe melons while ripe tissues reduce the efficacy of these gaseous ozone treatments, potentially by oxidative reaction with soluble refractive solids.     

Stable isotope dilution analysis of the Fusarium mycotoxins deoxynivalenol and 3-acetyldeoxynivalenol

Trichothecenes are secondary metabolites produced by several fungi of the Fusarium genus during their growth period. They inhibit protein biosynthesis in eukaryotic cells resulting in numerous toxic effects such as diarrhea, vomiting, and gastro-intestinal inflammation. Considering its occurrence in food and feedstuff, deoxynivalenol (DON) is one of the most important trichothecenes. We report the synthesis of stable isotope labeled 15-d(1)-deoxynivalenol (15-d(1)-DON) from its natural precursor 3-acetyldeoxynivalenol (3-AcDON) as starting material. Furthermore, a method for the analysis of DON and 3-AcDON using HPLC-MS/MS with stable isotope labeled 15-d(1)-DON and 3-d(3)-AcDON as internal standards has been developed. In total, 18 cereal product samples were analyzed with contamination levels ranging from 10-301 microg/kg for DON and 5-14 microg/kg for 3-AcDON. This is the first report of an isotope dilution MS method for the analysis of type B-trichothecenes.     

Development of stable haploid strains and molecular genetic tools for Naumovozyma castellii (Saccharomyces castellii)

The budding yeast Naumovozyma castellii (syn. Saccharomyces castellii) has been included in comparative genomics studies and functional analyses of centromere DNA elements, and has been shown to possess beneficial traits for telomere biology research. To provide useful tools for molecular genetic approaches, we produced stable haploid heterothallic strains from an early ancestral strain derived from the N. castellii collection strain CBS 4310. To this end, we deleted the gene encoding the Ho endonuclease, which is essential for the mating type switching. Gene replacement of HO with the kanMX3 resistance cassette was performed in diploid strains, followed by sporulation and tetrad microdissection of the haploid spores. The mating type (MATa or MATα) was determined for each hoΔ mutant, and was stable under sporulation‐inducing conditions, showing that the switching system was totally non‐functional. The hoΔstrains showed wild‐type growth rates and were successfully transformed with linear DNA using the general protocol. Opposite mating types of the hoΔstrains were mated, resulting in diploid cells that efficiently formed asci and generated viable spores when microdissected. By introduction of a point mutation in the URA3 gene, we created a uracil auxotrophic strain, and by exchanging the kanMX3 cassette for the hphMX4 cassette we show that hygromycin B resistance can be used as a selection marker in N. castellii. These haploid strains containing genetic markers will be useful tools for performing genetic analyses in N. castellii. Moreover, we demonstrate that homology regions of 200–230 bp can be successfully used for target site‐specific integration into genomic loci. Copyright © 2016 John Wiley & Sons, Ltd.     

Reflectance study of compressed paper

Reflectance of three paper grades was investigated using a pressure gauge, an imaging system, and a spectrophotometer. It was observed that under high pressure, dark-colored areas appear in paper when using the imaging system. The area of the dark pattern increases as the pressure is raised. Reflectance measurements as a function of wavelength confirmed that the dark patterns appear due to the contact of the paper and the probe window of the pressure gauge. Based on results by other researchers the amount and the nature of optical contact observed depends on structural properties of paper such as surface roughness, formation, and compressibility. Although there are differences in the spectral properties of different paper grades they all share the common feature that the reflectance is decreasing as a function of the applied pressure. An empiric result for the fine and the super-calendered (SC) paper samples measured is that they both have an exponential dependence of total reflectance as a function of applied pressure when the illumination wavelength is kept constant.     

Characterization of starch based nanocomposites

The goal of this study was to characterize the nanostructure and the properties of starch based nanocomposites with either cellulose nano whiskers (CNW) or layered silicates (LS) (synthetic hectorite) as reinforcements. Modified potato starch was used as matrix with water and sorbitol as plasticizers and with 5 wt.% of either of the reinforcements. Two methods were explored to prepare samples for transmission electron microscopy (TEM) examination; chemical fixation and freeze etching. It was possible to characterize the nanostructure both parallel and perpendicular to the nanocomposite surface by the freeze etching technique. Both nanocomposites showed well-distributed reinforcements in the starch matrix. Dynamic mechanical thermal analysis showed that the storage modulus was significantly improved at elevated temperatures, especially for the layered silicate nanocomposite. Both nanocomposites showed a significant improvement in tensile properties compared to the pure matrix.     

The effect of pre-softened wood chips on wood fibre aspect ratio and mechanical properties of wood–polymer composites

The objective of this work was to study the effect of chemical pre-treatment and moisture content of wood chips on the wood particle aspect ratio after compounding in a twin-screw extruder and on the mechanical properties of wood–polymer composites (WPCs). Composites with 50 wt.% wood content were manufactured using pre-treated and untreated wood chips. The effect of wood moisture content on composite properties was studied by using dried and undried wood chips. The mechanical properties and fracture surfaces of the composites as well as the microstructure and aspect ratio of wood particles after compounding were studied. The highest wood particle aspect ratio after extrusion was achieved by using pre-treated, undried wood chips as raw material. The chemical pre-treatment was found to enhance the defibration of wood chips as well as the mechanical properties of the composites.     

Assessing design features of virtual keyboards for text entry

OBJECTIVE: The present research examined design of a virtual keyboard for text entry with a rotary controller, emphasizing users who differ in age and system experience. BACKGROUND: Existing research has minimally addressed usage frequency, age, and the effects of display shape and letter arrangement on movement and visual search components of text entry tasks. The present research was conducted to close these gaps. METHOD: Two experiments were completed to examine younger (18-28 years) and older (60-75 years) adults' movement and visual search capabilities using four keyboard shapes and three keyboard arrangements. In a third experiment examining combined effects on shape design, 32 younger (18-28 years) and 32 older (60-75 years) adults entered words on the two best shapes from the first experiments. RESULTS: For the movement task, movement time was lowest for shapes with higher shape-controller compatibility. For the visual search task, search time and accuracy were best on the alphabetic arrangement. In the combined task, shape did not significantly influence performance at different levels of practice. Transfers, however, suggested that the shape with salient visual features elicited a text entry strategy for older adults that may promote more consistent performance under occasional usage. CONCLUSION: The studies together demonstrate that keyboard shape is important for efficient performance. Shape-controller compatibility facilitated performance in both age groups. Salient features facilitate performance, especially for older adults. In nearly all cases alphabetic arrangement yielded the best performance. APPLICATION: Recommendations are provided for virtual keyboard design for different usage frequencies, contexts, and users.