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71.
Mariusz Andrzejczuk Marcin Rasiski Agata Roguska Marcin Pisarek Malgorzata Lewandowska 《Microscopy research and technique》2019,82(3):173-177
To characterize complex, three‐dimensional nanostructures, modern microscopy techniques are needed, such as electron tomography and focused ion beam (FIB) sectioning. The aim of this study was to apply these two techniques to characterize TiO2 nanotubes in terms of their size, shape, volume, porosity, geometric surface area, and specific surface area (SSA). For these experiments, titania nanotubes were fabricated by means of the electrochemical oxidation of titanium at a voltage of 20 V for 2 hr followed by heat treatment at 450°C for 3 hr to change the amorphous structure into a crystalline anatase structure. The quantitative data obtained from the FIB and electron tomography reconstructions show a high similarity in porosity and some differences in SSA. These might be the result of differences in resolution between the two reconstruction techniques. 相似文献
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Rebecca L. Tarnopol Sierra Bowden Kevin Hinkle Krithika Balakrishnan Akira Nishii Caleb J. Kaczmarek Tara Pawloski Dr. Anthony G. Vecchiarelli 《Chembiochem : a European journal of chemical biology》2019,20(20):2535-2545
One of the primary challenges facing synthetic biology is reconstituting a living system from its component parts. A particularly difficult landmark is reconstituting a self-organizing system that can undergo autonomous chromosome compaction, segregation, and cell division. Here, we discuss how the syn3.0 minimal genome can inform us of the core self-organizing principles of a living cell and how these self-organizing processes can be built from the bottom up. The review underscores the importance of fundamental biology in rebuilding life from its molecular constituents. 相似文献
74.
Weronika Gonciarz ukasz Lechowicz Mariusz Urbaniak Wiesaw Kaca Magdalena Chmiela 《International journal of molecular sciences》2021,22(1)
Infections due to Gram-negative bacteria Helicobacter pylori may result in humans having gastritis, gastric or duodenal ulcer, and even gastric cancer. Investigation of quantitative changes of soluble biomarkers, correlating with H. pylori infection, is a promising tool for monitoring the course of infection and inflammatory response. The aim of this study was to determine, using an experimental model of H. pylori infection in guinea pigs, the specific characteristics of infrared spectra (IR) of sera from H. pylori infected (40) vs. uninfected (20) guinea pigs. The H. pylori status was confirmed by histological, molecular, and serological examination. The IR spectra were measured using a Fourier-transform (FT)-IR spectrometer Spectrum 400 (PerkinElmer) within the range of wavenumbers 3000–750 cm−1 and converted to first derivative spectra. Ten wavenumbers correlated with H. pylori infection, based on the chi-square test, were selected for a K-nearest neighbors (k-NN) algorithm. The wavenumbers correlating with infection were identified in the W2 and W3 windows associated mainly with proteins and in the W4 window related to nucleic acids and hydrocarbons. The k-NN for detection of H. pylori infection has been developed based on chemometric data. Using this model, animals were classified as infected with H. pylori with 100% specificity and 97% sensitivity. To summarize, the IR spectroscopy and k-NN algorithm are useful for monitoring experimental H. pylori infection and related inflammatory response in guinea pig model and may be considered for application in humans. 相似文献
75.
Mariusz Ciesielski Bohdan Mochnacki 《International Journal of Cast Metals Research》2019,32(4):213-220
The thermal processes proceeding in the casting-mould system are analysed. Solidification process can be described in the different ways. One of them consists in the application of the Fourier–Kirchhoff equation in which the source function controlling the phase change is introduced. In this paper, two approaches to the modelling of pure metals solidification, i.e. the solidification proceeding at the constant temperature and in the artificially introduced interval of temperature are considered. Numerical model used at the stage of computations is constructed using the Control Volume Method in which the principle of conservation of energy (taking also into account the release of latent heat of solidification) is applied to each control volume ensuring the correctness of the model. The use of Voronoi/Thiessen tessellation to the discretization of the casting-mould system can accurately reproduce the shape of each sub-domain. In the final part of the paper, the examples of computations are shown. 相似文献
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Mariusz Banaszkiewicz 《Materials at High Temperatures》2019,36(4):354-367
The principal objective of work was to develop fast and accurate method for online creep life monitoring of steam turbine rotors. For this purpose, a characteristic strain model of creep was investigated and validated using test data of 2%CrMoV rotor steel. The model was then used to determine creep damage functions which are introduced to compute creep damage at a constant temperature based on the Robinson time fraction rule. Finite element (FE) creep calculations of a turbine rotor were performed using the same creep model to obtain reference damage results for validating the proposed method. Comparisons of the results obtained using both methods showed a very good accuracy of the online creep damage predictions. 相似文献
78.
Pazera Marcin Buciakowski Mariusz Witczak Marcin Mrugalski Marcin 《Neural computing & applications》2020,32(2):379-389
Neural Computing and Applications - The paper is devoted to the problem of a neural network-based robust simultaneous actuator and sensor faults estimator design for the purpose of the fault... 相似文献
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Krzysztof Letachowicz Tomasz Gołębiowski Mariusz Kusztal Waldemar Letachowicz Wacław Weyde Marian Klinger 《Hemodialysis international. International Symposium on Home Hemodialysis》2015,19(4):E21-E23
We report a case of long‐term uneventful catheter use in a patient with previous recurrent vascular access dysfunction and infection. A single‐lumen tunneled catheter was inserted into the left internal jugular vein after a failed attempt of dual‐lumen permanent catheter placement. The follow‐up since device implantation has exceeded 5 years without any complications related to vascular access. 相似文献