Characterization of interphase adhesion in multi-layer coating systems

Multi-layer coating systems are the state of the art for painting of metal and plastic surfaces e.g. in the automotive industry. Utilizing such systems consisting of several specialized coating materials, a high adhesion and a good corrosion protection can be reached, along with good barrier properties, chemical and scratch resistance as well as appealing surface appearance. The interphase regions between single layers of such systems represent areas of a potential weakness, where a delamination and a premature coating degradation can start. To test in a short time the protective properties of the coating, a special hydrothermal cyclic loading test procedure was developed, which causes accelerated build-up of internal stress within the coating system. Using an electrochemical impedance spectroscopy with various optimized electrode geometries, it was possible to detect the early stages of coating degradation and to compare different coating systems. Internal stress measurements contributed additionally to a better understanding of the coating degradation.     

Use of grape by‐product as a source of dietary fibre and phenolic compounds in sourdough mixed rye bread

The study evaluated the effect of grape by‐products (GP) on the chemical composition, soluble (SDF) and insoluble (IDF) dietary fibre, phenolic compounds and antioxidant activity (AA) and organoleptic characteristics of sourdough mixed rye bread. The following samples of sourdough mixed rye bread were prepared: control bread (BC) and breads with GP at four different levels: 4%, 6%, 8% and 10%. Addition of GP significantly improves dietary fraction contents, as bread with a 10% addition of GP accounts for 39% and 37% higher contents of IDF and SDF than BC. The assay of radical‐scavenging activity and reducing ability showed that GP addition greatly enhanced antioxidant properties of mixed rye breads. Profiles of phenolic compounds of supplemented breads were dominated by procyanidin B1 and B2, catechin, epicatechin, caffeic acid and myricetin. With an increase in the level of GP, the hardness and gumminess of the bread significantly increase. Although both BC and supplemented breads showed common volatile compound profiles, there were slight differences in the concentrations of those components. Sensory evaluation of GP‐enhanced breads revealed that a maximum of 6% GP could be incorporated to prepare acceptable products.     

Characterization of catalysts obtained from rapidly quenched alloy precursors by electrochemical/chemical processes of material degradation—selected examples

Rapidly quenched amorphous alloys—containing metallic or metalloid elements—are precursors for selective catalysts of many technically important reactions. To increase their activity, various methods of material degradation occurring at the surface and in the bulk of the rapidly quenched alloys have been used for promoting the catalytic performance of such materials. The modifications of the structure, composition, and morphology of the substrate proved to be efficient in transforming inactive metal alloy precursors into active and selective catalysts for hydrogenation, and dehydrogenation of organic compounds, as well as for other processes like steam reforming of methanol. This article presents several examples of characterization of such catalysts and discusses their selectivity and activity in a connection with physical and chemical properties of their surfaces. Moreover, it is shown that scanning electron microscopy, Auger electron spectroscopy, scanning Auger microscopy, and energy dispersive spectrometry allowed the local changes occurring during the activation process to be identified and their implications for catalytic function to be considered.     

Liver graft bile duct necrosis--indication for retransplantation

The Herlitz type of junctional epidermolysis bullosa (H-JEB) is a severe blistering disease affecting the skin and mucous membranes, which is usually lethal within the first year of life. The laminin 5 genes have been implicated as candidate genes for most patients with H-JEB. Recently, two hotspot mutations were delineated in the LAMB3 gene, known as R42X and R635X, and have been noted in over 50% of mutant LAMB3 alleles. Here, we present a case of H-JEB of Hungarian origin with a neonatal lethal outcome. Monoclonal antibody staining showed a lack of expression of the laminin 5 beta 3 chain, as a possible result of a mutation in one of the laminin 5 genes. Screening of the family identified the previously described mutation R635X in exon 14 of LAMB3 in each of the parents and one healthy sibling in the heterozygous form, while proband was homozygous for R635X, and the other sibling proved to be genotypically normal. These results underscore the widespread prevalence of R635X in H-JEB cases from around the world.     

Extraction of diffuse objects from images by means of watershed and region merging: example of solar images.

This paper presents a new method for extraction of diffuse objects from images, which was developed for segmentation of solar images obtained from extreme-UV imaging telescope (EIT) experiments of the satellite SOHO mission. As a particular type of objects to be extracted coronal holes in EIT images have been chosen. The method described is based on the use of a watershed algorithm. The result of the watershed segmentation is a partition of the whole domain of the image into a large number of small regions. These regions are then combined in a region merging process. The proposed region merging algorithm iteratively adds the darkest regions and maximizes the average contrast between a current mask and a set of its neighboring regions. We show that the maximization of the average contrast gives segmentation results that are visually acceptable. Furthermore, this approach allows us to conduct the segmentation of EIT images independently of any explicit fine-tuning parameters. The proposed method was extensively tested on EIT images obtained at various times and various levels of solar activity, and we will show that it can be used independently of the local brightness level and the extent of coronal holes.     

Secure Distributed Key Generation for Discrete-Log Based Cryptosystems

A Distributed Key Generation (DKG) protocol is an essential component of threshold cryptosystems required to initialize the cryptosystem securely and generate its private and public keys. In the case of discrete-log-based (dlog-based) threshold signature schemes (ElGamal and its derivatives), the DKG protocol is further used in the distributed signature generation phase to generate one-time signature randomizers (r = g^k). In this paper we show that a widely used dlog-based DKG protocol suggested by Pedersen does not guarantee a uniformly random distribution of generated keys: we describe an efficient active attacker controlling a small number of parties which successfully biases the values of the generated keys away from uniform. We then present a new DKG protocol for the setting of dlog-based cryptosystems which we prove to satisfy the security requirements from DKG protocols and, in particular, it ensures a uniform distribution of the generated keys. The new protocol can be used as a secure replacement for the many applications of Pedersen's protocol. Motivated by the fact that the new DKG protocol incurs additional communication cost relative to Pedersen's original protocol, we investigate whether the latter can be used in specific applications which require relaxed security properties from the DKG protocol. We answer this question affirmatively by showing that Pedersen's protocol suffices for the secure implementation of certain threshold cryptosystems whose security can be reduced to the hardness of the discrete logarithm problem. In particular, we show Pedersen's DKG to be sufficient for the construction of a threshold Schnorr signature scheme. Finally, we observe an interesting trade-off between security (reductions), computation, and communication that arises when comparing Pedersen's DKG protocol with ours.     

N2- and C8-substituted oligodeoxynucleotides with enhanced thrombin inhibitory activity in vitro and in vivo

2'-Deoxyguanosine (G) analogues carrying various hydrophobic substituents in the N2 and C8 positions were synthesized and introduced through solid-phase synthesis into 15-mer oligodeoxynucleotide, GGTTGGTGTGGTTGG, which forms a chairlike structure consisting of two G-tetrads and is a potent thrombin inhibitor. The effects of the substitutions at N2 and C8 of the G-tetrad-forming G residues on the thrombin inhibitory activity are relatively small, suggesting that these substitutions cause relatively small perturbations on the chairlike structure formed by the oligodeoxynucleotide. Introduction of a benzyl group into N2 of G6 and G11 and naphthylmethyl groups into N2 of G6 increased the thrombin inhibitory activity, whereas other substituents in these positions had almost no effect or decreased the activity. Particularly, the oligodeoxynucleotide carrying a 1-naphthylmethyl group in the N2 position of G6 showed an increase in activity by about 60% both in vitro and in vivo. Substitutions on the N2 position of other G residues had little effect or decreased the activity. Introduction of a relatively small group, such as methyl and propynyl, into the C8 positions of G1, G5, G10, and G14 increased the activity, presumably due to the stabilization of a chairlike structure, whereas introduction of a large substituent group, phenylethynyl, decreased the activity, probably due to the steric hindrance.     

A new static field solver with open boundary conditions in the3D-CAD-system MAFIA

The numerical solution of Maxwell's equations involves calculation in a finite volume enclosing the structure of interest. Using simple Dirichlet or Neumann boundary conditions causes inaccuracies or requires excessively large meshes. For a static problem, which can be reduced to a scalar potential problem that can be described by Poisson's equation, it is shown that one can formulate more accurate boundary conditions. A multipole expansion of the well-known solutions of the Poisson equation can be used to yield better boundary values from the neighboring inner potentials. This modified formulation is used in solving electrostatic and magnetostatic problems with the recently developed solver of the 3-D CAD system MAFIA, which solves Maxwell's equations for a very broad range of applications