Surgical sperm retrieval and intracytoplasmic sperm injection as treatment of obstructive azoospermia

Male genital tract obstructions may result from infections, previous inguinal and scrotal surgery (vasectomy) and congenital bilateral absence of the vas deferens (CBAVD). Microsurgery can sometimes be successful in treating the obstruction. In other cases and in cases of failed surgical intervention, the patient can be treated by microsurgical or percutaneous epididymal sperm aspiration (MESA, PESA) or testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). We present the results of 39 ICSI procedures for obstructive azoospermia in 24 couples. The aetiology of the obstruction was failed microsurgery in 11 patients, CBAVD in nine and genital infections in four. Sperm retrieval was accomplished via MESA in four cases, PESA in 18 cases and via TESE in 11 cases. TESE was only applied when PESA failed to produce enough spermatozoa for simultaneous ICSI. In six patients, the ICSI procedure was performed with cryopreserved spermatozoa after an initial PESA procedure. Fertilization occurred in 47% of the metaphase II oocytes; embryo transfer was performed in 92% of procedures and resulted in a clinical pregnancy in 13/39 procedures. Ongoing pregnancy was achieved in 10/39 procedures. One pregnancy was terminated early after prenatal investigation showed a cytogenetic abnormality (47,XX+18, Edwards syndrome). The other nine pregnancies resulted in the live birth of 10 children, without any congenital abnormalities. Epididymal and testicular retrieved spermatozoa were successfully used for ICSI to treat obstructive azoospermia, and resulted in an ongoing pregnancy in 10 of 24 couples (41.6%) after 39 ICSI procedures, a success rate of 25.6% per treatment cycle and of 27.7% per embryo transfer.     

Pollen identification

LEARNING OBJECTIVES: The purpose of this review is to introduce the reader to a systematic approach to categorizing pollen types. This will enable the reader to recognize pollen characteristics of the most common botanical sources and determine the relevant contributors to the local aeroallergen burden. DATA SOURCES: Allergenic plant and pollen atlases for pollen characteristics, allergy texts for procedures and overviews, and relevant reviews from the English medical literature (1985 to present). RESULTS: By applying a visual gestalt utilizing grain number, size, shape, surface structures, and internal detail, one is able to identify pollen source to the appropriate botanical taxonomic level. Identification may be possible only to the family or order, but most frequently to the genus, and occasionally to the species. CONCLUSIONS: Outdoor sampling allows the identification of aeroallergen burden in a locale. In conjunction with field work, the relevant sources can be identified as well as time of pollination. This allows the physician to correlate symptoms with exposure and relevant sensitization.     

A priority media access control protocol for video communication support on CSMA/CD LANs

Real-time multimedia communication applications demand performance requirements which differ significantly from conventional data communication applications. Current local area networks (LANs) provide efficient transport for bursty data traffic; however, they cannot necessarily provide quality of service guarantees for real-time communications. In this work we introduce and investigate an experimental priority protocol for supporting real-time communication on Ethernet, a popular implementation of multiple-access broadcast bus LANs. We examine the new protocol, known as Priority Mode-CSMA/CD (PM-CSMA/CD), providing a high priority (HP) class with CSMA/CD employed for standard priority (SP) traffic. PM-CSMA/CD performance is examined through computer simulation of videotelephony workstations operating over the shared bus LAN. Over all observed traffic conditions, the priority protocol provides performance satisfying real-time packet transport requirements of audio and video streams. The primary advantage of our protocol over similar priority schemes is its physical layer compatibility with standard CSMA/CD. The protocol also provides improved channel utilization with increasing high priority load, with no penalty in SP class performance at low to medium network loads.     

Opportunities in silicon photovoltaics and defect control in photovoltaic materials

Silicon is the dominant semiconductor material in use for terrestrial photovoltaic cells. The critical issues in cell design, materials specification and cell processing are reviewed. Specific attention is given to light trapping, minority carrier lifetime control and novel microstructures for enhancing photon absorption. Relevant topics in generic defect control are discussed with particular emphasis on compound semiconductor materials.     

Channel access and interference issues in multi-code DS-CDMA wireless packet (ATM) networks

Multi-Code Direct-Sequence Code-Division-Multiple-Access (MC-CDMA) has been proposed as a flexible multiple access scheme for wireless packet networks that support a large variety of mobiles with different and even time-varying rates. Using MC-CDMA, traffic streams with significantly different transmission rates can be easily integrated into a unified architecture, with all the transmissions occupying the same bandwidth and having the same spread spectrum processing gain. In this paper, we address medium-access and interference issues in MC-CDMA wireless packet networks. For medium access, we propose and study Multi-Code CDMA (MC-CDMA) with Distributed-Queueing Request Update Multiple Access (DQRUMA) to form a unified bandwidth-on-demand fair-sharing platform for multi-rate wireless services. DQRUMA is an efficient demand-assignment multiple access protocol for wireless access and scheduling. Pseudo-Noise (PN) codes (primary codes) and optimal power levels are allocated to the mobiles on a slot-by-slot basis, and a Maximum Capacity Power Allocation (MCPA) criterion exploits the sub-code concatenation property of the MC-CDMA transmission. Simulation results show that the system provides close to ideal-access performance for multi-rate mobiles, both with homogeneous traffic characteristics and with a mix of heterogeneous traffic characteristics. Finally, we analyze the effects of MC-CDMA intercell interference on the reverse link (i.e., mobile to cell site) and investigate interference reduction by using the Maximum Capacity Power Allocation (MCPA) criterion. Our results show significant reduction in reverse-link MC-CDMA intercell interference is possible using the MCPA criterion.     

The economic potential of rural water supply projects in Tanzania

The rationale behind the decision to invest in the development of rural water supply in Tanzania is discussed. It is shown that theoretically economic as well as social benefits are supposed to influence investment decisions. In practice, however, choice of projects to be developed are made solely on the basis of social rather than economic factors. Economic potential of such projects are generally not considered. Examples of small scale irrigation, fish farming and village afforestation are discussed. It is concluded that economic potential of rural water supply projects should be considered together with the social benefits during the planning stages of such projects.     

Anaerobic toxicity and biodegradability of pulp mill waste constituents

Batch bioassays have been conducted to characterize the response of methanogenic bacteria to several constituents of sulfite evaporator condensate. The results can be grouped into three ranges with increasingly severe consequences to anaerobic reactors: a low concentration, no effect range; a medium concentration range where methanogenesis is temporarily interrupted or slowed down, but may return to normal; and a high concentration range where methanogenesis is permanently inhibited. In some cases the toxicant was metabolized when present in the lower concentration ranges. There was also evidence that mechanisms other than fermentation to methane were significant in accounting for removal of the toxicants from solution. Organisms acclimated to low concentrations of a toxicant are better able to withstand a shock load of that toxicant than are unacclimated organisms.     

Founder BRCA1 and BRCA2 mutations in Ashkenazi Jews in Israel: frequency and differential penetrance in ovarian cancer and in breast-ovarian cancer families

Germ-line BRCA1 and BRCA2 mutations account for most of familial breast-ovarian cancer. In Ashkenazi Jews, there is a high population frequency (approximately 2%) of three founder mutations: BRCA1 185delAG, BRCA1 5382insC, and BRCA2 6174delT. This study examined the frequency of these mutations in a series of Ashkenazi women with ovarian cancer unselected for family history, compared with the frequency of these mutations in families ascertained on the basis of family history of at least two affected women. Penetrance was compared, both according to the method of family ascertainment (i.e., on the basis of an unselected ovarian cancer proband vs. on the basis of family history) and for the BRCA1 founder mutations compared with the BRCA2 6174delT mutation. There was a high frequency (10/22; [45%]) of germ-line mutations in Ashkenazi women with ovarian cancer, even in those with minimal or no family history (7/18 [39%]). In high-risk Ashkenazi families, a founder mutation was found in 59% (25/42). Families with any case of ovarian cancer were significantly more likely to segregate a founder mutation than were families with site-specific breast cancer. Penetrance was higher in families ascertained on the basis of family history than in families ascertained on the basis of an unselected proband, but this difference was not significant. Penetrance of BRCA1 185delAG and BRCA1 5382insC was significantly higher than penetrance of BRCA2 6174delT (hazard ratio 2.1 [95% CI 1.2-3.8]; two-tailed P = .01). Thus, the high rate of germ-line BRCA1/BRCA2 mutations in Ashkenazi women and families with ovarian cancer is coupled with penetrance that is lower than previously estimated. This has been shown specifically for the BRCA2 6174delT mutation, but, because of ascertainment bias, it also may be true for BRCA1 mutations.     

Continuum based modeling of silicon integrated circuit processing: An object oriented approach

Continuum based integrated circuit process modeling is the dominant tool used to investigate and understand integrated circuit (IC) development. This paper describes the commonly used models for implantation, diffusion, and material growth. In addition, the supporting numerical techniques are described. This paper focuses on the implementation in object oriented code, Florida Object Oriented Process Simulator (FLOOPS). The software architecture is described for implementing models and numerics. A number of process examples are introduced and discussed.