Distributed optical fibre sensors based on spontaneous Brillouin scattering employing multimode Fabry-Perot lasers

Proposed and characterised is the use of multi-longitudinal mode Fabry-Perot laser sources, in distributed optical fibre sensors based on spontaneous Brillouin scattering, for simultaneous strain and temperature measurements. Experimental results show that, by using such multi-longitudinal mode lasers, it is possible to measure the Brillouin spectrum, even if the overall laser spectral linewidth is larger than the Brillouin frequency shift. In this case, a suitable receiver scheme, combining optical and electrical heterodyne detection, is needed. Results report an increase in the threshold value of the input peak power before onset of nonlinear effects, allowing for a better sensing performance compared to single-longitudinal mode lasers.     

Genetically engineered plasmonic nanoarrays

In the present Letter, we demonstrate how the design of metallic nanoparticle arrays with large electric field enhancement can be performed using the basic paradigm of engineering, namely the optimization of a well-defined objective function. Such optimization is carried out by coupling a genetic algorithm with the analytical multiparticle Mie theory. General design criteria for best enhancement of electric fields are obtained, unveiling the fundamental interplay between the near-field plasmonic and radiative photonic coupling. Our optimization approach is experimentally validated by surface-enhanced Raman scattering measurements, which demonstrate how genetically optimized arrays, fabricated using electron beam lithography, lead to order of ten improvement of Raman enhancement over nanoparticle dimer antennas, and order of one hundred improvement over optimal nanoparticle gratings. A rigorous design of nanoparticle arrays with optimal field enhancement is essential to the engineering of numerous nanoscale optical devices such as plasmon-enhanced biosensors, photodetectors, light sources and more efficient nonlinear optical elements for on chip integration.     

“真实的”建筑VS世界危机

如果说2008年北京奥运会将中国介绍给了全世界,那么,2010年上海世博会无疑将世界介绍给了中国。     

A new photoactive building block for investigation of DNA backbone interactions: photoaffinity labeling of human DNA polymerase beta

The cross-linking of target proteins or nucleic acids to light-activatable ligands is an important tool for elucidating molecular interactions. Through the use of photoaffinity-labeling reagents, several new insights into nucleic acid interactions have been obtained, for example in DNA replication and repair. In most known photoprobes, the applied light-sensitive functionalities are placed directly at the nucleobase or are attached via linkers to either the nucleobase or the phosphate backbone. Here we describe the first photoprobe that bears a light-sensitive aryl(trifluoromethyl)diazirine at the sugar moiety of a DNA oligonucleotide. We devised a route for the synthesis of the modified nucleoside and its incorporation into an oligonucleotide. The photoactive species was proven to be stable under the conditions employed in routine automated DNA synthesis. The modified oligonucleotide was shown by subsequent photolabeling studies of human DNA polymerase beta to form a covalent complex to the enzyme upon irradiation with near-UV light.     

Remediation of waters contaminated with MCPA by the yeasts Lipomyces starkeyi entrapped in a sol-gel zirconia matrix

A single-stage sol-gel route was set to entrap yeast cells of Lipomyces starkeyi in a zirconia (ZrO(2)) matrix, and the remediation ability of the resulting catalyst toward a phenoxy acid herbicide, 4-chloro-2-methylphenoxyacetic acid (MCPA), was studied. It was found that the experimental procedure allowed a high dispersion of the microorganisms into the zirconia gel matrix; the ZrO(2) matrix exhibited a significant sorption capacity of the herbicide, and the entrapped cells showed a degradative activity toward MCPA. The combination of these effects leads to a nearly total removal efficiency (>97%) of the herbicide at 30 °C within 1 h incubation time from a solution containing a very high concentration of MCPA (200 mg L(-1)). On the basis of the experimental evidence, a removal mechanism was proposed involving in the first step the sorption of the herbicide molecules on the ZrO(2) matrix, followed by the microbial degradation operated by the entrapped yeasts, the metabolic activity of which appear enhanced under the microenvironmental conditions established within the zirconia matrix. Repeated batch tests of sorption/degradation of entrapped Lipomyces showed that the removal efficiency retained almost the same value of 97.3% after 3 batch tests, with only a subsequent slight decrease, probably due to the progressive saturation of the zirconia matrix.     

Comparative numerical study of FEM methods solving gas damping in perforated MEMS devices

Three different numerical strategies are presented for the estimation of the damping force acting on perforated movable MEMS dampers. Results from the 2D Perforated Profile Reynolds (PPR) method and the simplified 2D ANSYS method are compared with accurate full 3D flow simulations. Altogether, 32 different topologies are compared varying, e.g., the dimensions of the square damper and the square holes, and the number of holes. The case of uniform perforation and perpendicular motion is studied. Oscillation in the low frequency regime is assumed, that is, the compressibility and inertia of the gas are ignored in the study. While the PPR method is in good agreement with the 3D simulations, the forces given by the ANSYS method were considerably smaller. The reasons for this are studied, and a compact expression to explain the small forces is derived.     

Carbohydrate moieties on the in vitro immunoreactivity of soy β-conglycinin

β-Conglycinin is a functional glycoprotein and one of the most important soybean allergens. The aim of the present research was to investigate the role of the N-glycans moieties of β-conglycinin on its in vitro immunoreactivity. The soy allergen was obtained by isoelectric precipitation from commercial soy protein isolate and was enzymatically deglycosylated by PNGase F (Peptide N-Glycosidase F EC 3.5.1.52). In order to optimize deglycosylation conditions different reaction times and allergen concentrations were tested. The extent of deglycosylation was estimated by SDS–PAGE, CZE, RP-HPLC, and MALDI-TOF MS analyses, which provided information related to changes in protein structure. The antigenicity of both native β-conglycinin and its deglycosylated form was evaluated by western-blotting and indirect ELISA employing polyclonal rabbit anti-soybean sera and horseradish peroxidase-labeled goat anti-rabbit IgG while the in vitro allergenicity was assessed by means of indirect competitive inhibition ELISA employing human sera (IgE) of soy allergics. β-Conglycinin was effectively deglycosylated by PNGase F. Data on immunological tests suggested that glycosyl moieties forming this glycoprotein might be involved in its immunoreactivity.     

Liquid electrolyte positioning along the device channel influences the operation of Organic Electro-Chemical Transistors

In this work, we show the influence of the liquid electrolyte adsorption by porous films made of poly(3,4-ethylenedioxythiophene)–poly(styrenesulfonate), PEDOT:PSS, on the operation of an Organic Electro-Chemical Transistor with an active channel based on these polymeric films. In particular, the effect of film hydration on device performance is evaluated by studying its electrical response as a function of the spatial position between the electrolyte and the channel electrodes. This is done by depositing a PEDOT:PSS film on a super-hydrophobic surface aimed at controlling the electrolyte confinement next to the electrodes. The device response shows that the confinement of ionic liquids near to the drain electrode results in a worsening of the current modulation. This result has been interpreted in the light of studies dealing with the transport of ions in semiconducting polymers, indicating that the electrolyte adsorption by the polymeric film implies the formation of liquid pathways inside its bulk. These pathways, in particular, affect the device response because they are able to assist the drift of ionic species in the electrolyte towards the drain electrode. The effect of electrolyte adsorption on the device operation is confirmed by means of moving-front measurements, and is related to the reproducibility of the device operation curves by measuring repeatedly its electrical response.     

A reactive and dependable transport protocol for wireless mesh networks

In the last few years, several different mesh network architectures have been conceived by both industry and academia; however, many issues on the deployment of efficient and fair transport protocols are still open. One of these issues is rate adaptation, that is, how to allocate the network resources among multiple flows, while minimizing the performance overhead. In order to address this problem, in this paper, we first define an analytical framework for a very simple topology. The model allows us to study the performance of an adaptive and responsive transport protocol when the effect of the lower layers are ignored. The mathematical approach alone does not represent a feasible solution, but it contributes to determining the strengths and weaknesses of our proposal. The main novelty of the proposed solution is that the congestion control approach is based on a hop-by-hop mechanism, which allows nodes to adapt their transmitting rates in a distributed way and to keep track of dynamic multi-hop network characteristics in a responsive manner. This is in contrast with classical literature solutions, founded on an end-to-end support. Anyway, to ensure the reliability, a coarse-grained end-to-end algorithm is integrated with the proposed hop-by-hop congestion control mechanism to provide packet level reliability at the transport layer. Performance evaluation, via extensive simulation experiments, shows that the proposed protocol achieves a high performance in terms of network throughput.