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41.
Summary Random-coiled conformation of poly(L-leucine), which has -branched side-chain, was theoretically analyzed by a conformational energy calculation based on intra-residue interactions. Calculated characteristic ratio 7.62 was obtained by using the transformation matrix statistically averaged over the entire side-chain conformational space of L-Leu residue. This value is smaller than those of poly(L-phenylalanine) (11.24) and poly(L-tyrosine) (12.33) which have -branched side-chain. 相似文献
42.
Hata N Kim MH Isoda K Kino-oka M Kawase M Yagi K Taya M 《Journal of Bioscience and Bioengineering》2004,97(4):233-238
Anchorage-dependent cultivation of human epithelial and keratinocyte cells was carried out on surfaces modified with synthesized dendrimers. Notable elongation of the epithelial cells was recognized on the culture surface immobilized with a dendrimer having D-glucose as a functional ligand, but not when a dendrimer having L-glucose was used or when the dendrimer was ligand-free. This morphological change was attributable to a temporary grasping of the cells at the D-glucose moiety via a glucose transporter-mediated mechanism present in the cell membrane. Following visualization of the actin filaments of the cells, it was considered that the cellular elongation on the D-glucose-bound dendrimer surface reflected the degree of formation of the cellular cytoskeleton. The cellular roundness was calculated by means of image analysis of the individual cells and employed as a parameter to evaluate the formation of the cellular cytoskeleton. In the culture of keratinocytes on the D-glucose-bound dendrimer surface, it was demonstrated that the decrease in the ratio of elongated cells (i.e., cells with a low roundness value) was correlated with the deterioration in the growth potential associated with cellular senescence. 相似文献
43.
Yukio Kagawa Tadakuni Murai Osamu Matsumoto 《International journal for numerical methods in engineering》1983,19(3):315-329
Two kinds of techniques for solving a shape determination problem are proposed. The determination of the interface boundary between two domains governed by Poisson and Laplace equations under the compatible and constraint condition is considered. Influence coefficient and inverse variational approaches are examined by using the iterative finite element procedure. A two-dimensional model of a junction-type field effect transistor is a test example. The determination of its interface boundary and the prediction of the potential distribution and static characteristic are demonstrated. 相似文献
44.
Summary
Poly(N-hydroxyethyl-L-glutamine) (PHEG) hydrogels were prepared by aminolysis of
poly( -benzyl L-glutamate) with 2-aminoethanol and hydrophobic or hydrophilic
crosslinkers, and the effect of the hydrophobicity of the crosslinkers was evaluated.
The swelling properties, tensile properties and enzymatic degradation behavior were
studied in phosphate buffered saline (PBS). The swelling ratio and degradation rate of
these hydrogels were highly dependent on the hydrophobicity of the crosslinkers,
while tensile properties were dependent on the swelling ratio, but not on the
hydrophobicity of the crosskinkers. 相似文献
45.
Mehta DS Hinosugi H Saito S Takeda M Kurokawa T Takahashi H Ando M Shishido M Yoshizawa T 《Applied optics》2003,42(4):682-690
The maximum measurable range of a spectral interference microscope depends on the coherence length of the light transmitted by its tunable spectral filter. To achieve a large range in step-height measurement we have developed a new tunable spectral filter that uses tandem liquid-crystal Fabry-Perot interferometers (LC-FPIs), which can simultaneously attain both a high spectral resolution and a large tuning range. Fringe visibility measurements were carried out, and it was found that the coherence length of the light transmitted through tandem LC-FPIs is two times larger than that transmitted through a single LC-FPI. Using this novel tunable spectral filter, we developed a new spectral interference microscope for the measurement of three-dimensional shapes of discontinuous objects. Experimental results of step-height measurements both with a single LC-FPI and with tandem LC-FPIs are presented for a combination of standard steel gauge block sets with 1-, 99-, and 100-microm steps. A large range (1-100 microm) of measurement with submicrometer resolution was achieved with tandem LC-FPIs that was not possible with our previous system in which a single LC-FPI was used. 相似文献
46.
Saccharomyces cerevisiae a-agglutinin, which is involved in mating and covalently anchoring to the cell wall, consists of two components, Aga1p and Aga2p, whose syntheses are individually regulated. To facilitate the analysis of the protein-protein interaction on agglutination between a- and alpha-agglutinins, the construction of a yeast strain (MATa) with the functional protein prepared by genetic fusion of Aga1p- and Aga2p-encoding genes and by the expression system using the UPR-ICL promoter derived from the n-alkane-assimilating yeast, Candida tropicalis, which is functional under the condition of lower glucose concentration was tried and the agglutination ability of the constructed strain was evaluated with a yeast strain (MATa) which expressed AGalpha1 encoding alpha-agglutinin under the control of the same promoter. The genes were integrated into the yeast chromosomes. Cell agglutination between both (MATa) strains was observed microscopically when these two strains were mix-cultured to a glucose-decreased concentration. The agglutination was further confirmed by the sedimentation test and by the quantification using a filter. These results proved that the constructed Aga1p-Aga2p fusion protein was enoughly functional for the interaction with the Agalpha1 protein, and that this phenomenon occurred dependent on glucose concentration, but independent of the peptide pheromones secreted by the cells of the opposite mating types. Using this system, the role of two disulphide linkages between Aga1p and Aga2p on the binding activity between Aga2p and Aga1p was first evaluated. Under the treatment by the SH-compound (dithiothreitol), in which Agalpha2p is easily released into the medium from the intact cell surface, the Aga1p and Aga2p fusion protein was a good tool to make clear the role of the disulphide linkages. As a result, the linkages had a significant effect on not only the assembly but also the binding activity. The novel and simple system described here may further facilitate the study of molecular interaction in agglutination. 相似文献
47.
Ken’ichiro Kita Masaki Narisawa Atsushi Nakahira Hiroshi Mabuchi Masayoshi Itoh Masaki Sugimoto Masahito Yoshikawa 《Journal of Materials Science》2010,45(1):139-145
The polymer blends of PCS (polycarbosilane) and PMHS-h (polymethylohydrosiloxane with high molecular weight) were prepared
by freeze-drying process of mixed benzene solution. Melt viscosity, mass loss, and gas evolution from prepared polymer blends
were analyzed. A polymer blend of HSah15 (15 mass% PMHS-h to PCS) was melt-spun to fiber form, curing by thermal oxidation
and pyrolyzed at various temperatures up to 1773 K. The obtained fibers were investigated by tensile tests, FE-SEM (field
emission scanning electron microscope) observation, and XRD (X-ray diffraction) analysis. After pyrolysis at 1273 K, there
were no pores in the cross section of the fiber derived from pure PCS; however, there were amounts of pores in the cross sections
of the fiber derived from HSah15. After pyrolysis at 1773 K, the coarse β-SiC (silicon carbide) crystals were formed on the
outside surface of the fiber derived from pure PCS; however, no remarkable β-SiC crystal were formed on the outside surface
of the fiber derived from HSah15. 相似文献
48.
Akiko Ishikura Akitoshi Mizuno Masahito Watanabe Tadahiko Masaki Takehiko Ishikawa Shinji Kohara 《Journal of the American Ceramic Society》2007,90(3):738-741
High-energy synchrotron X-ray diffraction experiments were performed for clathrate-forming barium–germanium (Ba–Ge) alloys in the liquid state near the eutectic composition. The accurate structure information of highly reactive melts has been obtained by applying electrostatic levitation technique as a containerless method. The structure information obtained from the reverse Monte Carlo simulation suggests that the short-range ordering between the Ge atoms occurs with the addition of Ba atoms in the liquid Ba–Ge alloys. This can be associated with the formation of the cage-like structure composed of the Ge atoms in the liquid alloys near the eutectic composition. 相似文献
49.
Kohashi T. Kitamura A. Murai M. Usukura T. Watanabe Y. Horii N. 《Selected Areas in Communications, IEEE Journal on》1985,3(4):574-583
The Universal Link System belongs to the "loop subsystem" of a local area network (LAN). This system has circuit-switching facilities and packet-switching facilities, and uses a local area network as an integrated switching system. The Universal Link System is well suited for a very wide range of uses, with the merits of both circuit switching and packet switching. The Outline of this system is described below. 相似文献
50.
A new type of millimeter-wave imaging technique, passive millimeter-wave microscopy is proposed and demonstrated. Passive millimeter-wave imaging techniques when combined with scanning near-field microscopy enable direct observation of millimeter-wave signals thermally emitted from the viewed object with a spatial resolution below the diffraction limit. Details of our passive microscope system operating at Ka-band frequencies are explained. Millimeter-wave images obtained with the system are presented to demonstrate the feasibility of this new imaging technique. 相似文献