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71.
Kitako Tabata Eriko Sugano Akito Hatakeyama Yoshito Watanabe Tomoya Suzuki Taku Ozaki Tomokazu Fukuda Hiroshi Tomita 《International journal of molecular sciences》2021,22(13)
The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on retinal ganglion cells after transduction of the optogenetic gene mVChR1 using an adeno-associated virus vector. Rats were exposed to continuous light for a week, and visually evoked potentials (VEPs) were recorded. The intensities of continuous light (500, 1000, 3000, and 5000 lx) increased substantially after VEP recordings. After the final recording of VEPs, retinal ganglion cells (RGCs) were retrogradely labeled with a fluorescein tracer, FluoroGold, and the number of retinal ganglion cells was counted under a fluorescent microscope. There was no significant reduction in the amplitudes of VEPs and the number of RGCs after exposure to any light intensity. These results indicated that RGCs were photosensitive after the transduction of optogenetic genes and did not induce any phototoxicity by continuous light exposure. 相似文献
72.
Masashi Imanaka Shigeo Tomita Suguru Kanda Mitsuteru Fujieda Kimikazu Sasa Jens Olaf Pepke Pedersen Hiroshi Kudo 《Journal of aerosol science》2010,41(5):468-474
To investigate the contribution of ions to gas nucleation, we have performed experiments on the formation of water droplets in H2O/N2 and H2O/Ar gas mixtures by irradiation with a 20 MeV proton beam and by positive corona discharge. The size of the formed nanoparticles was measured using a differential mobility analyzer equipped with a Faraday cup electrometer. Using the proton beam, droplets around 10 nm in diameter were observed for both positively and negatively charged particles, but none were found when the corona discharge was used. This implies the importance of the presence of both positive and negative ions for the formation of nanosize droplets, which attract each other by Coulomb interactions, enhancing the collision frequency and leading to the formation of the 10 nm droplets. 相似文献
73.
Theoretical investigations have been carried out to predict the non-axisymmetric buckling seen in the throat of circular elastic-plastic tubes subjected to a nosing operation along a frictionless conically shaped die. The buckling points and associated modes are determined by Hill's bifurcation theory in conjunction with a non-axisymmetric buckling mode. The results show that increasing the die semi-angle and the tube thickness or decreasing the material work-hardening rate are always beneficial to improve the buckling limit. The associated critical buckling mode number generally decreases as the tube thickness increases and does not depend drastically on the die semiangle nor on the hardening rate. The effect of the average r value is substantial and the buckling limit is improved when the r value reduces. Part of the present results predict well the results observed in existing experimental work. 相似文献
74.
Masaaki Bannai Yasushi Tomita Yasushi Ishida Takahiko Miyazaki Atsushi Akisawa Takao Kashiwagi 《Energy》2007
Energy service business, or energy service company (ESCO), is expanding among industrial users as a means of energy saving. The ESCO business normally tends to become a long-term operation. During the operation, fluctuations of fuel and electricity costs significantly impact on the stability of the profit from ESCO business. Therefore, it is essential to reduce the risk of fuel and electricity cost fluctuations. Generally, a transaction called “financial derivative” is used as a measure of hedging against the fuel price fluctuation. In the case of ESCO business, it is necessary to manage the risk of both electricity and fuel price fluctuations because the variation in electricity price strongly affects the profit from ESCO as that in fuel price does. 相似文献
75.
Satoshi Murakami Kazuhiro Satou Taturo Kijima Masataka Watanabe Taeko Izumi 《European Journal of Lipid Science and Technology》2011,113(4):450-458
The enantiopure of (R)‐(?) MαNPA was allowed to react with racemic 18‐(tert‐butyldimethylsilyloxy)‐5‐octadecayne‐7‐ol which was derived from dodecane‐1,12‐diol, yielding diastereomeric esters mixture. These racemic esters were easily separated by HPLC on silica‐gel. The absolute configurations of the first‐eluted diastereomeric esters from the separated esters were determined using 1H NMR anisotropy method. Analysis on 1H NMR spectra and HPLC elution time of the synthesized esters and those of MαNP ester derived from natural methyl‐12‐hydroxystealate showed that the absolute configuration of natural 12‐hydroxystearic acid was R form, and the enantiomeric excess was over 99%. 相似文献
76.
77.
Acid catalyzed reactions of 1-octene on TiO2 in sub- and supercritical water were investigated (T = 250-450 °C, P = 11-33 MPa). The main products were 2-octene and 2-octanol. Additionally, other liner C8 alkenes and liner secondary C8 alcohols were produced as by-products. Through kinetic analysis, acid catalyzed reactions can divide into the reaction catalyzed by Lewis acidic sites on TiO2 and the reaction catalyzed by protons produced by the dissociation of water molecules. Each type of the reaction is affected by water density or ionic product of water, respectively, therefore, reaction mechanism changes with temperature and pressure. From the contribution of each reaction type, the temperature dependence of cis/trans ratio of produced 2-octene could also be explained. 相似文献
78.
Kenji Imai Koji Takai Tatsunori Hanai Makoto Shiraki Yusuke Suzuki Hideki Hayashi Takafumi Naiki Youichi Nishigaki Eiichi Tomita Masahito Shimizu Hisataka Moriwaki 《International journal of molecular sciences》2014,15(7):11294-11306
Obesity-related metabolic abnormalities, including adipokine imbalance and chronic inflammation, are involved in liver carcinogenesis. Chemerin, a novel adipokine, plays a critical role in adipogenesis, energy metabolism, and inflammation. We evaluated the impact of serum chemerin levels on liver functional reserves in hepatocellular carcinoma (HCC) patients and on the recurrence and prognosis of HCC. This study included 44 patients with any stage of HCC who underwent curative treatment at Gifu Municipal Hospital (Gifu, Japan) between 2006 and 2007. Recurrence-free survival and overall survival were estimated using the Kaplan-Meier method. Serum albumin levels (Pearson’s correlation coefficient; r = 0.3110, p = 0.0399), platelet counts (r = 0.4159, p = 0.0050), and prothrombin times (r = 0.3775, p = 0.0115) were significantly correlated with serum chemerin levels in patients with HCC, and they were inversely correlated with Child-Pugh scores (r = −0.3732, p = 0.0126), serum alanine aminotransferase levels (r = −0.3864, p = 0.0105), and total bilirubin levels (r = −0.4023, p = 0.0068). Among these variables, a multiple comparison test identified that platelet counts and total bilirubin levels were associated with serum chemerin levels (p < 0.0083). No significant correlation was found between serum chemerin levels and recurrence-free survival (p = 0.3691) or overall survival (p = 0.7916). In HCC patients, serum chemerin concentrations were correlated with liver functional reserves and platelet counts, but not with recurrence or prognosis. 相似文献
79.
Summary Organocobalt poly(arylene-vinylene)s composed of 1,2- or 1,3- linkage at their cyclobutadiene moieties (3 and 4, respectively) were prepared by the Heck reaction of p-divinylbenzene with (cyclobutadiene)cobalt-containing monomers having two aryl bromide moieties (1 and 2, respectively). The number average molecular weights (M n) of the obtained organocobalt poly(arylene-vinylene)s (3 and 4) were 4,100 and 4,000, respectively (GPC, on the basis of polystyrene). UV-vis spectra of 3 and 4 exhibited main π-π* absorption peaks at 362 and 397 nm, respectively, which were ca. 60 nm bathochromically shifted from those of the corresponding monomeric units. Both polymers were found to show thermotropic liquid crystallinity in the range of room temperature to ca. 80 °C by employing differential scanning calorimetry (DSC) and the optical measurement using crossed polarizers. Received: 2 July 1999/Accepted: 29 July 1999 相似文献
80.
Guang-chao Chen Meiyong Liao Masataka Imura Kiyomi Nakajima Yoshimasa Sugimoto Yasuo Koide 《Diamond and Related Materials》2010,19(4):319-323
Strontium titanate (STO) films were directly deposited on Ib (100) single crystal diamond by r.f. magnetron sputtering. The as-deposited STO film was in amorphous state. On the other hand, the crystalline STO film was obtained under the optimized condition of a deposition temperature of 250 °C and a post-annealing temperature of 650 °C. STO/diamond junctions were fabricated on boron-doped homoepitaxial layers grown on p+-type single crystal diamond substrates. Electrical properties of the STO/diamond junction were investigated by changing the surface terminations of diamond with hydrogen or oxygen and the crystallinity of the STO film. It was found that the amorphous STO acted like a semi-insulator on H-diamond surface and that the amorphous STO/O-diamond junction behaved like a Schottky diode. The crystalline STO/O-diamond showed a complex rectifying behavior. The crystalline STO film possessed a higher dielectric constant as compared to that of the amorphous one. 相似文献