Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems

Background

Recently, manufactured nano/microparticles such as fullerenes (C₆₀), carbon black (CB) and ceramic fiber are being widely used because of their desirable properties in industrial, medical and cosmetic fields. However, there are few data on these particles in mammalian mutagenesis and carcinogenesis. To examine genotoxic effects by C₆₀, CB and kaolin, an in vitro micronuclei (MN) test was conducted with human lung cancer cell line, A549 cells. In addition, DNA damage and mutations were analyzed by in vivo assay systems using male C57BL/6J or gpt delta transgenic mice which were intratracheally instilled with single or multiple doses of 0.2 mg per animal of particles.

Results

In in vitro genotoxic analysis, increased MN frequencies were observed in A549 cells treated with C₆₀, CB and kaolin in a dose-dependent manner. These three nano/microparticles also induced DNA damage in the lungs of C57BL/6J mice measured by comet assay. Moreover, single or multiple instillations of C₆₀ and kaolin, increased either or both of gpt and Spi^- mutant frequencies in the lungs of gpt delta transgenic mice. Mutation spectra analysis showed transversions were predominant, and more than 60% of the base substitutions occurred at G:C base pairs in the gpt genes. The G:C to C:G transversion was commonly increased by these particle instillations.

Conclusion

Manufactured nano/microparticles, CB, C₆₀ and kaolin, were shown to be genotoxic in in vitro and in vivo assay systems.     

Temperature dependences of piezoelectric properties of vanadium substituted SrBi2Nb2O9 ceramics with grain orientation

The temperature dependence of the piezoelectric properties of vanadium substituted strontium bismuth niobate, SrBi₂Nb_1.95V_0.05O₉ (SBNV) ceramics, were investigated in various vibration modes. The effects of grain orientation in SBNV ceramics on the piezoelectric properties were also studied by the hot-forging (HF) method. The anisotropy of the piezoelectric properties of each vibration mode was confirmed by observing the grain orientation. In particular, HF-SBNV ceramics of the (33) and (15) modes showed excellent piezoelectric properties with relatively high mechanical quality factors, Q_m (2200, 4600), and high electrical quality factors, Q_{e max} (66.0, 21.6), respectively. In addition, HF-SBNV ceramics showed low temperature coefficients of resonance frequency TC-f_r (−16.5, −27.0). HF-SBNV ceramics are considered to be superior candidates for the lead-free piezoelectric application of ceramic resonators.     

Development of a novel microstructure fabrication method with co-axial dual capillary solution flow type droplet cells and electrochemical deposition

A new method for maskless fabrication of metallic patterns or structures on metals is described. A solution flow type droplet cell, with co-axial dual capillaries was applied to form fine metal structures such as strips and rods. This type of droplet cell enables movement of the cell during formation. Nickel fine patterns with a width of about 200 μm were formed on a Cu substrate. The width of the formed pattern does not change with the scanning speed of the cell, but the thickness of the formed pattern changes with the speed. Two different deposition modes were examined to form metal rods, one is a mold free deposition mode and the other is a mold assisted deposition mode. Both modes enable the formation of Ni rods, however, reproducibility of mold free deposition mode was not good. The mold assisted deposition mode has far better the reproducibility, because of the use of the inside wall of the 100 μm diameter inner tube as the mold. It is possible to form nickel micro-rods, about 100 μm in diameter and 12 mm long with relatively smooth surfaces by the mold assisted deposition mode.     

Preparation and properties of biodegradable network poly(ester-carbonate) elastomers

Biodegradable elastomeric network poly(ester-carbonate)s were prepared from multifunctional aliphatic carboxylic acids such as tricarballylic acid (Y_t) or trimesic acid (Y) and polycarbonate diols (PCD) with molecular weights of 1000 and 2000 g/mol. Prepolymers prepared by a melt polycondensation were cast from dimethylformamide solution and postpolymerized at 270 °C for 40-80 min to form a network. The resultant films were transparent, flexible and insoluble in organic solvents. WAXS exhibited the crystalline peaks due to polycarbonate segments for the network films from PCD₂₀₀₀, while those from PCD₁₀₀₀ were amorphous. The tensile properties were determined for these network films at the temperatures 22, 30, 40 and 50 °C. These films showed elastomeric properties at all temperatures measured. The elongation at break was much higher for the films from PCD₂₀₀₀ (208-434%) than those from PCD₁₀₀₀ (40-120%), and decreased with increasing temperatures. The weight losses of the network films degraded in the buffer solution of Rhizopus delemar lipase at 37 °C increased with time, suggesting that these network films are biodegradable. The degradation rate of the network films from Y_t is faster than that from Y. The GPC curves showed that the lipase hydrolyzed both the ester linkages between Y or Y_t and PCD as well as polycarbonate moiety in the network polymer.     

Use of a Metal-mould Process in the Development of Hemispherical Heavy-walled Castings

The exacting quality required of hemispherical heavy-walled steel castings, used in nuclear power applications, has been remarkably improved by the adoption of a newly developed metal-mould process. This technique is dependent on the use of a metal core. The optimum conditions for solidification were determined by the finite element analytical method. The appropriate wall-thickness of the chill elements was also investigated using data based on actual experience. The core was designed in such a manner that the surface carried a series of concave “dimples” in the manner of a golf-ball, the pattern being intended to prevent the formation of cracks, generated on the casting surface when in contact with a metal mould. Stresses caused by solidification shrinkage were reduced by splitting the core. In the event, the casting structure conformed well to the results of the solidification analysis. Radiographic examination revealed a high level of soundness, with no trace of internal defects. No anisotropy was detected in mechanical properties. It is shown that this process is being currently employed in the manufacture of actual products.     

Attractants for Rice Leaf Bug, <Emphasis Type="BoldItalic">Trigonotylus caelestialium</Emphasis> (Kirkaldy), are Emitted from Flowering Rice Panicles

Volatiles were extracted from rice plants of various growth stages with solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS) to identify attractants that cause invasion of the rice leaf bug Trigonotylus caelestialium (Kirkaldy) into paddy fields. The composition of volatile blends produced by rice plants changed with rice development. In addition, volatile blend compositions differed between the panicles and the stems and leaves. The relative geranyl acetone content was high in all plant structures analyzed. In volatiles from whole plants in the fourth-leaf stage and panicles in the full-ripe stage, the relative content of green leaf volatiles (GLVs) was higher than that found in other rice plant structures. In contrast, relative terpene levels emitted from whole plants in the panicle-formation stage and by panicles and stems and leaves in the flowering stage were higher than those of other rice plant structures. However, the type of terpenes found differed between the panicles and the stems and leaves. Relative levels of β-caryophyllene in whole plants in the panicle-formation stage and panicles in the flowering stage were much higher than that in stems and leaves in the flowering stage. Our previous studies demonstrated that the odor from whole plants in the panicle-formation stage and panicles in the flowering stage is attractive to rice leaf bugs. Here, the attractiveness of β-caryophyllene to adult bugs was investigated in olfactometer assays. Adult females were attracted to β-caryophyllene at a concentration of 0.001%, which is approximately equivalent to the concentration produced by flowering rice panicles. However, β-caryophyllene also was present in the odor of whole plants in the fourth-leaf stage and in stems and leaves in the flowering stage. Furthermore, the amounts of this compound emitted from these structures were similar. Therefore, we suggest that the relative abundance of this compound in a volatile blend is important for attractance of the bugs.