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991.
992.
Muniz-Miranda M Gellini C Salvi PR Schettino V Pagliai M Innocenti M 《Journal of nanoscience and nanotechnology》2011,11(10):8763-8767
Nanostructured Ag platforms have been obtained by simple chemical procedure and characterized by AFM (atomic force microscopy) measurements, for use in biosensing by means of SERS (surface-enhanced Raman scattering) spectroscopy. The SERS efficiency of these substrates has been verified by microRaman measurements on small RNA chains with different nucleobase content, showing sensitivity near attomole level. It is our opinion that these metal substrates may be widely used as appropriate sensors for detecting biomolecules in many applications concerning medical diagnostics, pharmacological research and nanomaterials technology. 相似文献
993.
Andrea Martinelli Massimo Calì Lucio D'Ilario Iolanda Francolini Antonella Piozzi 《应用聚合物科学杂志》2011,121(6):3368-3376
The effect of the holding temperature and time in the melt state of poly(L ‐lactide) (PLLA) samples on the nonisothermal melt crystallization process and on the structure have been investigated by means of DSC, polarized optical microscopy and wide angle X‐ray scattering. As standard starting material, single crystals grown from dilute solution were used. In the mild melting condition, the survived athermal nuclei favor high temperature polymer crystallization, while the more severe treatment leads the PLLA to crystallize at higher supercooling with a sporadic nucleation. At the intermediate melting temperature a distinct double nucleation mechanism was observed while at the lower nuclei concentration, a double crystallization rate was also found. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献
994.
Giovanni Bertoldo Maria Cristina Della Lucia Andrea Squartini Giuseppe Concheri Chiara Broccanello Alessandro Romano Samathmika Ravi Massimo Cagnin Andrea Baglieri Piergiorgio Stevanato 《International journal of molecular sciences》2021,22(13)
Sulfur is an essential plant macronutrient, and its adequate supply allows an efficient root storage and sugar extractability in sugar beets (Beta vulgaris L.). In this study, we investigated the effect of changes in sulfur availability on the endophytic community structure of sugar beets. Plants were hydroponically grown in a complete nutrient solution (S-supplied), a nutrient solution without MgSO4 (S-deprived), and a nutrient solution without MgSO4 for six days and resupplied with 100 μM MgSO4 for 48 h (S-resupplied). The sulfur status was monitored by inductively coupled plasma ICP–OES, and combustion analysis together with the evaluation of microRNA395 as a biomarker for sulfate status. Metabarcoding of the bacterial 16S rRNA gene was carried out in order to determine leaf endophytic community structure. The Shannon diversity index significantly differed (p < 0.05) between sulfate-supplied and sulfate-deprived seedlings. Validation by Real-Time PCR showed a significant increase (p < 0.05) of Burkholderia spp. in sulfate-deprived plants as compared to sulfate-supplied ones. The study sheds new light on the effects of nutrient deficiency on the microbiome of sugar beet plants. 相似文献
995.
996.
Ali Forootani Raffaele Iervolino Massimo Tipaldi Joshua Neilson 《IEEE/CAA Journal of Automatica Sinica》2020,7(4):975-990
A stochastic resource allocation model, based on the principles of Markov decision processes (MDPs), is proposed in this paper. In particular, a general-purpose framework is developed, which takes into account resource requests for both instant and future needs. The considered framework can handle two types of reservations (i.e., specified and unspecified time interval reservation requests), and implement an overbooking business strategy to further increase business revenues. The resulting dynamic pricing problems can be regarded as sequential decision-making problems under uncertainty, which is solved by means of stochastic dynamic programming (DP) based algorithms. In this regard, Bellman’s backward principle of optimality is exploited in order to provide all the implementation mechanisms for the proposed reservation pricing algorithm. The curse of dimensionality, as the inevitable issue of the DP both for instant resource requests and future resource reservations, occurs. In particular, an approximate dynamic programming (ADP) technique based on linear function approximations is applied to solve such scalability issues. Several examples are provided to show the effectiveness of the proposed approach. 相似文献
997.
998.
Giampiero Ferraguti Francesca Fanfarillo Luigi Tarani Giovanna Blacon Francesca Tarani Christian Barbato Antonio Minni Massimo Ralli Silvia Francati Antonio Greco Carla Petrella Marco Fiore 《International journal of molecular sciences》2022,23(21)
Infertility is a worldwide health issue defined by the World Health Organization (WHO) as the inability to establish a pregnancy after 12 months or more of regular and unprotected sexual intercourse. Male infertility etiology can be related to either congenital or acquired factors. The therapeutical approach to male infertility depends on the underlying causes and includes medical and surgical treatments. In recent studies, the potential role of nerve growth factor (NGF) in male reproductive physiology has been proposed. It has been hypothesized that neurotrophins might be involved in testis morphogenesis and regulation of several aspects of spermatogenesis. Moreover, it has been shown that NGF exerts its role on gonadotropin-releasing hormone (GnRH) neurons through the activation of the PKC/p–ERK1/2/p–CREB cascade, which leads to the activation of hypothalamic cells and the consequent activation of hypothalamus–pituitary–gonadal axis (HPG) with the secretion of GnRH. Lastly, it has been shown that the physiology of mature sperm is affected by both exogenous and endogenous NGF. The NGF impact on the HPG axis and its effect on GnRH neurons might be exploited in the therapy of male hypogonadism or used as a protective strategy against gonadal dysfunction related to chemotherapeutic agents. Moreover, the improving effect of NGF on sperm motility and vitality could be useful to enhance assisted reproduction outcomes. NGF could be supplemented to cryopreserved sperm samples to counteract the oxidative stress induced by the frozen and thawing processes. Indeed, the potential clinical applications of NGF in male infertility treatment have been discussed. 相似文献
999.
Alice Barinotti Massimo Radin Irene Cecchi Silvia Grazietta Foddai Elena Rubini Dario Roccatello Savino Sciascia 《International journal of molecular sciences》2022,23(22)
Chronic kidney disease (CKD) is a widely diffuse pathological condition which deeply impacts upon an affected patient’s quality of life and its worldwide rate is predicted to further rise. The main biological mechanism underlying CKD is renal fibrosis, a non-reversible process representing, for the affected system, a point of no return of tissue damage and dysfunction, deeply reducing the possible therapeutic strategies at the disposal of physicians. The best tool clinicians can use to address the extent of renal fibrosis at any level (glomeruli, tubule-interstitium, vasculature) is kidney biopsy that, despite its overall safety, remains an invasive procedure showing some shortcomings. Thus, the identification of novel non-invasive renal fibrosis biomarkers would be of fundamental importance. Here, when systematically reviewing the available evidence on serological biomarkers associated with renal fibrosis evaluated in patients suffering from CKD in the last five years, we found that despite the presence of several promising biomarkers, the level of observed evidence is still very scattered. Probably, the use of multiple measures capable of addressing different aspects involved in this condition would be the most suitable way to capture the high complexity characterizing the renal fibrotic process, having consequently a great impact on clinical practice by maximizing prevention, diagnosis, and management. 相似文献
1000.
Andrea Palermo Laura Giannotti Benedetta Di Chiara Stanca Franco Ferrante Antonio Gnoni Paola Nitti Nadia Calabriso Christian Demitri Fabrizio Damiano Tiziano Batani Massimo Lungherini Maria Annunziata Carluccio Biagio Rapone Erda Qorri Antonio Scarano Luisa Siculella Eleonora Stanca Alessio Rochira 《International journal of molecular sciences》2022,23(23)
Edentulism is the condition of having lost natural teeth, and has serious social, psychological, and emotional consequences. The need for implant services in edentulous patients has dramatically increased during the last decades. In this study, the effects of concentrated growth factor (CGF), an autologous blood-derived biomaterial, in improving the process of osseointegration of dental implants have been evaluated. Here, permeation of dental implants with CGF has been obtained by using a Round up device. These CGF-coated dental implants retained a complex internal structure capable of releasing growth factors (VEGF, TGF-β1, and BMP-2) and matrix metalloproteinases (MMP-2 and MMP-9) over time. The CGF-permeated implants induced the osteogenic differentiation of human bone marrow stem cells (hBMSC) as confirmed by matrix mineralization and the expression of osteogenic differentiation markers. Moreover, CGF provided dental implants with a biocompatible and biologically active surface that significantly improved adhesion of endothelial cells on CGF-coated implants compared to control implants (without CGF). Finally, data obtained from surgical interventions with CGF-permeated dental implants presented better results in terms of optimal osseointegration and reduced post-surgical complications. These data, taken together, highlight new and interesting perspectives in the use of CGF in the dental implantology field to improve osseointegration and promote the healing process. 相似文献