Phenotypic and genotypic characterization of Oenococcus oeni strains isolated from Italian wines

A phenotypic and genotypic characterization of 84 Oenococcus oeni isolates from Italian wines of different oenological areas was carried out. Numerical analysis of fatty acid profiles grouped the isolates into two clusters at low level of similarity (63%), the minor cluster containing seven isolates besides the type and the reference strains. Forthy-eight O. oeni isolates, representative of the two clusters, showed no differences in their metabolic properties (heterolactic fermentation pattern, citrate degradation capability and formation of some secondary metabolites). Moreover, the analysis of species-specific randomly amplified polymorphic DNA and 16S-23S rDNA intergenic spacer region polymorphism as well as the sequence-specific separation of V3 region from 16S rDNA by denaturing gradient gel electrophoresis demonstrated a substantial homogeneity among the isolates. On the basis of ApaI Pulsed Field Gel Electrophoresis (PFGE) restriction patterns, the 84 isolates were grouped into five different clusters at 70% similarity, but no correlation with the phenotypic groups could be demonstrated. However, by combining phenotypic and genotypic data, the 84 O. oeni isolates grouped into eight phenotypic-genotypic combined profiles and a relationship between the origin of the isolates and their combined profile became evident, so that a sort of strain specificity can be envisaged for each wine-producing area.     

Disaster-aware service provisioning with manycasting in cloud networks

Cloud services delivered by high-capacity optical datacenter networks are subject to disasters which may cause large-area failures, leading to huge data loss. Survivable service provisioning is crucial to minimize the effects of network/datacenter failures and maintain critical services in case of a disaster. We propose a novel disaster-aware service-provisioning scheme that multiplexes service over multiple paths destined to multiple servers/datacenters with manycasting. Our scheme maintains some bandwidth (i.e., degraded service) after a disaster failure vs. no service at all. We formulate this problem into a mathematical model which turns out to be an Integer Linear Program (ILP), and we provide heuristic optimization approaches as ILP is intractable for large problem instances. Numerical examples show that exploiting manycasting by intelligently selecting destinations in a risk-aware manner for service provisioning offers high level of survivability against link and node failures that may be caused by disasters and post-disaster failures at no extra cost compared to the other survivable schemes.     

A 0.18 μm CMOS direct down-converter for highly integrated 3G receivers

This paper presents the design of a high dynamic range direct down-converter for 3G cell-phone applications. The mechanisms responsible for second-order intermodulation distortion are discussed in details, leading to the following design strategy: the transconductor is degenerated by means of an RC filter, an LC network resonating at RF frequency loads the switching pair and carefully matched resistors are used in the output load. Prototypes realized in 0.18 μm CMOS show: +78 dBm IIP2 minimum among 40 samples, +10 dBm IIP3, 4 nV/√Hz input-referred noise density while burning only 4 mA from 1.8 V.     

Synthesis,size-dependent optoelectronic and charge transport properties of thieno(bis)imide end-substituted molecular semiconductors

The synthesis of two new thieno(bis)imide (TBI, N) end functionalized oligothiophene semiconductors is reported. In particular, trimer (NT3N) and pentamer (NT5N) have been synthesized and characterized. Two different synthetic approaches for their preparation were tested and compared namely conventional Stille cross coupling and direct arylation reaction via C–H activation. Theoretical calculations, optical and electrochemical characterization allowed us to assess the role of the π-conjugation extent, i.e., of the oligomer size on the optoelectronic properties of these materials. In both TBI ended compounds, due to the strong localization of the LUMO orbital on the TBI unit, the LUMO energy is almost insensitive to the oligomer size, this being crucial for the fine-tailoring of the energy and the distribution of the frontier orbitals. Surprisingly, despite its short size and contrarily to comparable TBI-free analogues, NT3N shows electron charge transport with mobility up to μ_N = 10⁻⁴ cm² V⁻¹ s⁻¹, while increasing the oligomer size to NT5N promotes ambipolar behavior and electroluminescence properties with mobility up to μ_N = 0.14 cm² V⁻¹ s⁻¹ and to μ_P = 10⁻⁵ cm² V⁻¹ s⁻¹.     

An Observational Theory for Mobile Ad Hoc Networks (full version)

We propose a process calculus to study the behavioural theory of Mobile Ad Hoc Networks. The operational semantics of our calculus is given both in terms of a Reduction Semantics and in terms of a Labelled Transition Semantics. We prove that the two semantics coincide. The labelled transition system is then used to derive the notions of (weak) simulation and bisimulation for ad hoc networks. The labelled bisimilarity completely characterises reduction barbed congruence, a standard branching-time and contextually-defined program equivalence. We then use our (bi)simulation proof method to formally prove a number of non-trivial properties of ad hoc networks.     

Path‐following algorithms and experiments for an unmanned surface vehicle

This paper addresses the problem of path following in two‐dimensional space for underactuated unmanned surface vehicles (USVs), defining a set of guidance laws at the kinematic level. The proposed nonlinear Lyapunov‐based control law yields convergence of the path‐following error coordinates to zero. Furthermore, the introduction of a virtual controlled degree of freedom for the target to be followed on the path removes singularity behaviors present in other guidance algorithms proposed in the literature. Some heuristic approaches are then proposed to face the problem of speed of advance adaptation based on path curvature measurement and steering action prediction. Finally a set of experimental results of all the proposed guidance laws, carried out with the Charlie USV, demonstrates the feasibility of the proposed approach and the performance improvements, in terms of precision in following the reference path and transient reduction, obtained by introducing speed adaptation heuristics. © 2009 Wiley Periodicals, Inc.     

A voltage controlled CMOS current divider with linear characteristic

A compact voltage controlled CMOS current divider is presented. Differently from previous implementations, exploiting the MOSFET translinear characteristics in subthreshold region, the proposed circuit is based on devices operating from moderate to strong inversion. An input current linearity range of nearly a decade is obtained by compensating for the deviations from the ideal drain current square-law approximation through an original empirical approach. The circuit is suitable for use as a building block in analog multipliers and for transconductor continuous tuning. The effectiveness of the proposed configuration is demonstrated by means of electrical simulations, performed on a prototype designed with a commercial process. The robustness of the linearization algorithm with respect to temperature variations is proven.     

Low-enthalpy geothermal energy: An opportunity to meet increasing energy needs and reduce CO2 and atmospheric pollutant emissions in Piemonte,Italy

The scope for diffusion of very low-enthalpy geothermal plants in the Piemonte region of Italy, using groundwater heat pumps (GWHP), was analyzed to check environmental sustainability and the benefits in terms of reducing greenhouse gas emissions from fossil fuels. GWHP implementation seemed particularly suitable to the specific characteristics of the Piemonte plain. An important thick and productive shallow aquifer is present across the entire plain beneath the major energy users and is therefore appropriate for geothermal energy development purposes. The building stock could be adapted to heat pumps in different ways, but objective-oriented policies will be required to reach the best results in terms of environmental benefits.     

Design,estimation and experimental validation of optical Polarization Mode Dispersion Compensator in 40 Gbit/s NRZ and RZ optical systems

Polarization Mode Dispersion (PMD) compensation has been a matter of investigation of several papers in literature. The proposed solutions belong basically to two large families: electronic compensators and optical compensators. Both PMD compensator schemes have advantages and disadvantages: electronic PMD compensators are usually simple to include in line-terminal, potentially low-cost, very fast, and FEC compatible but their development is strongly dependent on IC technology capability that at this time allows device developments up to the 10 Gbit/s bit rates area. Furthermore, they are strongly dependent on modulation formats, and they can operate only on a single channel. Conversely, optical compensators are independent of bit rate and modulation format and potentially they can compensate more channels simultaneously; their major drawbacks are the longer response time and the complexity in the feedback signal process within the control algorithm. In this paper we consider an optical Polarization Mode Dispersion Compensator (PMDC) that is simple to realize and easy to include at limited costs in each EDFA module (distributed compensation) as well as a single-stage front-end compensator. Numerical analysis of the PMDC and experimental results confirm the utility of the PMDC proposed and its capability in compensating DGD larger than 20 ps in NRZ and RZ 40 Gbit/s optical systems.     

Pathogenic Mutations Shift the Equilibria of α‐Synuclein Single Molecules towards Structured Conformers

α‐synuclein (α‐Syn) is an abundant brain protein whose mutations have been linked to early‐onset Parkinson's disease (PD). We recently demonstrated, by means of a single‐molecule force spectroscopy (SMFS) methodology, that the conformational equilibrium of monomeric wild‐type (WT) α‐Syn shifts toward β‐containing structures in several unrelated conditions linked to PD pathogenicity. Herein, we follow the same methodology previously employed for WT α‐Syn to characterize the conformational heterogeneity of pathological α‐Syn mutants A30P, A53T, and E46K. Contrary to the bulk ensemble‐averaged spectroscopies so far employed to this end by different authors, our single‐molecule methodology monitored marked differences in the conformational behaviors of the mutants with respect to the WT sequence. We found that all the mutants have a much higher propensity than the WT to adopt a monomeric compact conformation that is compatible with the acquiring of β structure. Mutants A30P and A53T show a similar conformational equilibrium that is significantly different from that of E46K. Another class of conformations, stabilized by mechanically weak interactions (MWI), shows a higher variety in the mutants than in the WT protein. In the A30P mutant these interactions are relatively stronger, and therefore the corresponding conformations are possibly more structured. The more structured and globular conformations of the mutants can explain their higher propensity to aggregate with respect to the WT.