Inhibition of Very Long Chain Fatty Acids Synthesis Mediates PI3P Homeostasis at Endosomal Compartments

A main characteristic of sphingolipids is the presence of a very long chain fatty acid (VLCFA) whose function in cellular processes is not yet fully understood. VLCFAs of sphingolipids are involved in the intracellular traffic to the vacuole and the maturation of early endosomes into late endosomes is one of the major pathways for vacuolar traffic. Additionally, the anionic phospholipid phosphatidylinositol-3-phosphate (PtdIns (3)P or PI3P) is involved in protein sorting and recruitment of small GTPase effectors at late endosomes/multivesicular bodies (MVBs) during vacuolar trafficking. In contrast to animal cells, PI3P mainly localizes to late endosomes in plant cells and to a minor extent to a discrete sub-domain of the plant’s early endosome (EE)/trans-Golgi network (TGN) where the endosomal maturation occurs. However, the mechanisms that control the relative levels of PI3P between TGN and MVBs are unknown. Using metazachlor, an inhibitor of VLCFA synthesis, we found that VLCFAs are involved in the TGN/MVB distribution of PI3P. This effect is independent from either synthesis of PI3P by PI3-kinase or degradation of PI(3,5)P₂ into PI3P by the SUPPRESSOR OF ACTIN1 (SAC1) phosphatase. Using high-resolution live cell imaging microscopy, we detected transient associations between TGNs and MVBs but VLCFAs are not involved in those interactions. Nonetheless, our results suggest that PI3P might be transferable from TGN to MVBs and that VLCFAs act in this process.     

SOCRATE: an optical bench dedicated to the understanding and improvement of a laser conditioning process

We present an automatic excimer laser bench (SOCRATE) allowing for the treatment of optical components by laser conditioning. This apparatus, developed at the Commissariat a l'Energie Atomique-Le Ripault, has been designed to add to this conditioning process an in situ, accurate laser-induced damage threshold (LIDT) measurement and different nondestructive optical techniques for the characterization of the component during treatment. Through different examples, we demonstrate the importance of these characterizations to improve the understanding of the laser conditioning. The role of an in situ adapted metrology associated in real time with a laser conditioning bench offers new opportunities to analyze laser-induced damage mechanisms and subsequently to increase the LIDT of optical components.     

Multiscale transparent electrode architecture for efficient light management and carrier collection in solar cells

The challenge for all photovoltaic technologies is to maximize light absorption, to convert photons with minimal losses into electric charges, and to efficiently extract them to the electrical circuit. For thin-film solar cells, all these tasks rely heavily on the transparent front electrode. Here we present a multiscale electrode architecture that allows us to achieve efficiencies as high as 14.1% with a thin-film silicon tandem solar cell employing only 3 μm of silicon. Our approach combines the versatility of nanoimprint lithography, the unusually high carrier mobility of hydrogenated indium oxide (over 100 cm(2)/V/s), and the unequaled light-scattering properties of self-textured zinc oxide. A multiscale texture provides light trapping over a broad wavelength range while ensuring an optimum morphology for the growth of high-quality silicon layers. A conductive bilayer stack guarantees carrier extraction while minimizing parasitic absorption losses. The tunability accessible through such multiscale electrode architecture offers unprecedented possibilities to address the trade-off between cell optical and electrical performance.     

Melt Homogenization and Self-Organization in Chalcogenides-Part II

The compositional variation of the non-reversing enthalpy at T_g, ΔH_nr(x), in Ge_xSe_100−x glasses decreases abruptly by an order of magnitude as x increases to x_c(1) = 19.5(5)%, the rigidity transition, and then remains minuscule till x increases to x_c(2) = 26.0(5)%, when the term abruptly increases by an order of magnitude as glasses become stressed-rigid. The rigid but unstressed networks formed in between these two transitions represent the Intermediate Phase (IP). The square-well like variation of ΔH_nr(x), also known as the reversibility window develops sloping walls, then a triangular shape and eventually disappears as glasses of increasing heterogeneity are studied. The ΔH_nr term ages over weeks outside the IP but not inside the IP. Raman line shapes of as-quenched melts are quite similar to those of T_g-cycled glasses for compositions in the IP, but not outside the IP– an optical analog of the thermal reversibility window. Variations of Molar volumes, display a global minimum in the IP and a pronounced increase outside that phase. Physical behavior of dry and homogeneous chalcogenide glasses that leads to sharp elastic and chemical phase transitions remains to be understood theoretically. The physics of network may be even more interesting than hitherto recognized.     

Optimising end-of-life system dismantling strategy

The management of end-of-life systems is becoming a major concern for systems manufacturers, as the negative impact of these systems on the environment is a matter of increasing public awareness, and their appropriate treatment offers economic opportunities. In this context, the disassembly of these systems in order to recycle their components is a possible and sound option that can make it possible to sustain economical progress while respecting environment requirements. The work undertaken in this paper considers modelling and optimising issues of such disassembly activities. An integrated approach is proposed to model and optimise the selection of valuable components of end-of-life systems, their recycling options and the way to obtain them. Because the framework of such problems is highly uncertain, we propose the use of Bayesian networks and their extension in terms of influence diagrams as mathematical tools for structuring and managing uncertainties. With this approach, one can take into account uncertainties rising from different sources on one hand and as a support for optimisation on the other hand.     

Recovering gallium from residual bayer process liquor

Gallium is normally obtained by direct electrolysis as a by-product from Bayer process residual liquor at an aluminum processing plant. However, to permit any net accumulation of the metal, the gallium concentration must be at least about 0.3 g/l in the liquor. This article describes a continuous process of extraction with organic solvents and rhodamine-B, followed by a re-extraction step into aqueous media. The final product is a solid containing up to 18 wt.% Ga in a solid mixture of hydroxides and oxides of gallium and aluminum. This final product can then be electrolyzed to recover the gallium more efficiently.     

STEP-NC compliant process planning of additive manufacturing: remanufacturing

Additive manufacturing is becoming one of the key methods for reproducing repair sections in remanufacturing processes. The major advantage of using additive processes is to minimize production time and waste. However, the surface quality and shape accuracy are usually insufficient for the final product because the approximated representation format causes the accumulation of the error during the geometric operations of the process planning. This limitation is a barrier to utilize additive processes as finishing processes, such as general metal cutting. There is need to improve the final quality of parts obtained with additive manufacturing. In this paper, STEP-based numerical control (STEP-NC)-based process planning is applied to the additive manufacturing. ISO 14649 (STEP-NC) describes part programs with geometric data directly and also contains the information necessary for the intelligent process planning. This paper proposes the STEP-NC-based representation method of additive manufacturing and the series of geometric reasoning to automate the derivation of the repair section. The proposed representation has the benefits to provide a high accuracy for the final surface and to describe multiple materials. Topological data maintain low error during the series of process planning through the CAD-CAM-CNC chain. The proposed platform supports consideration of the process tolerance and comparison of the selected plan with alternative processes. In order to show the practical advantages, an analysis of the remanufacturing process is carried out. The case study of remanufacturing a pocket part is presented in order to validate the proposed process plan. The result of the case study shows the improvement in terms of automatic process planning and surface quality accuracy.     

Moisture balance assessment at room scale for four cases based on numerical simulations of heat–air–moisture transfers for a realistic occupancy scenario

The moisture balance at room scale is influenced by the air change rate, moisture production and vapour transfer with the envelope. However, results may differ strongly from one study to another depending on the sizing of the ventilation system, the boundary conditions and the modelling of vapour transfer in the walls. This paper aims to provide a realistic comparison of usual construction techniques based on energy consumption, indoor comfort and durability. To achieve this objective, an existing whole-building heat–air–moisture simulation tool was selected to compute coupled transfer at room scale over an entire year. Moisture production due to occupancy was modelled using a stochastic approach. Four cases were selected to emphasize the differences of both a vapour-permeable wall assembly and a relative humidity sensitive ventilation system compared to common practices.     

Same Neighbourhood … Different Views? A Confrontation of Internal and External Neighbourhood Reputations

Residents and non-residents are likely to think differently about a neighbourhood's reputation. Relatively little is known about the similarities and differences between these internal and external types of neighbourhood reputation or the relationship between reputations and ‘real’ or ‘objective’ neighbourhood characteristics. This paper addresses two points: first, the extent to which neighbourhood reputations differ between and within groups; second, the extent to which these neighbourhood reputations are associated with measured neighbourhood characteristics. Data from a specially designed survey carried out in 24 neighbourhoods in Utrecht, the fourth largest city in the Netherlands, are used. Analysis of the data showed that neighbourhood reputations are rated higher by residents and estate agents than by other city residents. Within the group of other city residents, differences were found in how neighbourhood reputations are rated by socio-economic status, ethnicity and educational background. Further, it was found that neighbourhood reputations are correlated with measured social characteristics of the neighbourhood, while physical and functional neighbourhood characteristics are of less importance.     

The effect of external flow on the feeding currents of sessile microorganisms

Microscopic sessile suspension feeders live attached to surfaces and, by consuming bacteria-sized prey and by being consumed, they form an important part of aquatic ecosystems. Their environmental impact is mediated by their feeding rate, which depends on a self-generated feeding current. The feeding rate has been hypothesized to be limited by recirculating eddies that cause the organisms to feed from water that is depleted of food particles. However, those results considered organisms in still water, while ambient flow is often present in their natural habitats. We show, using a point-force model, that even very slow ambient flow, with speed several orders of magnitude less than that of the self-generated feeding current, is sufficient to disrupt the eddies around perpendicular suspension feeders, providing a constant supply of food-rich water. However, the feeding rate decreases in external flow at a range of non-perpendicular orientations due to the formation of recirculation structures not seen in still water. We quantify the feeding flow and observe such recirculation experimentally for the suspension feeder Vorticella convallaria in external flows typical of streams and rivers.