Involvement of GPR17 in Neuronal Fibre Outgrowth

Characterization of new pharmacological targets is a promising approach in research of neurorepair mechanisms. The G protein-coupled receptor 17 (GPR17) has recently been proposed as an interesting pharmacological target, e.g., in neuroregenerative processes. Using the well-established ex vivo model of organotypic slice co-cultures of the mesocortical dopaminergic system (prefrontal cortex (PFC) and substantia nigra/ventral tegmental area (SN/VTA) complex), the influence of GPR17 ligands on neurite outgrowth from SN/VTA to the PFC was investigated. The growth-promoting effects of Montelukast (MTK; GPR17- and cysteinyl-leukotriene receptor antagonist), the glial cell line-derived neurotrophic factor (GDNF) and of two potent, selective GPR17 agonists (PSB-16484 and PSB-16282) were characterized. Treatment with MTK resulted in a significant increase in mean neurite density, comparable with the effects of GDNF. The combination of MTK and GPR17 agonist PSB-16484 significantly inhibited neuronal growth. qPCR studies revealed an MTK-induced elevated mRNA-expression of genes relevant for neuronal growth. Immunofluorescence labelling showed a marked expression of GPR17 on NG2-positive glia. Western blot and RT-qPCR analysis of untreated cultures suggest a time-dependent, injury-induced stimulation of GPR17. In conclusion, MTK was identified as a stimulator of neurite fibre outgrowth, mediating its effects through GPR17, highlighting GPR17 as an interesting therapeutic target in neuronal regeneration.     

Hydrogen Sulfide Metabolite,Sodium Thiosulfate: Clinical Applications and Underlying Molecular Mechanisms

Thiosulfate in the form of sodium thiosulfate (STS) is a major oxidation product of hydrogen sulfide (H₂S), an endogenous signaling molecule and the third member of the gasotransmitter family. STS is currently used in the clinical treatment of acute cyanide poisoning, cisplatin toxicities in cancer therapy, and calciphylaxis in dialysis patients. Burgeoning evidence show that STS has antioxidant and anti-inflammatory properties, making it a potential therapeutic candidate molecule that can target multiple molecular pathways in various diseases and drug-induced toxicities. This review discusses the biochemical and molecular pathways in the generation of STS from H₂S, its clinical usefulness, and potential clinical applications, as well as the molecular mechanisms underlying these clinical applications and a future perspective in kidney transplantation.     

Optimized etching of porcelain and polycrystalline alumina with a glass phase

Chemical and thermal etching techniques are commonly used in ceramography to enhance microstructural features. While thermal etching works well for high purity ceramic systems, this technique may not be appropriate where glass is present. Porcelain and industrial alumina both contain a glass phase, typically 40?60 vol% and 4?30 vol%, respectively, and these glass chemistries are proposed to be similar. Chemical etching of porcelain is common, but the images published in the literature are frequently over-etched. When glass is present in the grain boundaries of alumina, thermal etching can cause the glass to disappear or to recrystallize, obscuring the microstructure. Because of this, it is proposed that both chemical and thermal etching are necessary to prepare an industrial alumina microstructure for grain size measurements. In addition, it was observed that chemical etching is sensitive to the residual stress in the glass phase, becoming more aggressive when there is residual tension in the glass.     

Status and perspectives of renewable energy policy and deployment in the European Union—What is needed to reach the 2020 targets?

This article evaluates the status of current RES deployment, policies and barriers in the EU-27 member states and compares it to the required to meet the 2020 targets. The evaluation relies strongly on the quantitative deployment status and policy effectiveness indicators. European RES deployment and policy has progressed strongly in recent years, but the growth here has been mainly driven by effective policies in a small or medium number of top runner countries. Across Europe, the highest average policy effectiveness over six years was reached for onshore wind (4.2%), biofuels (3.6%) and biomass electricity (2.7%), while in the heat sector, all technologies score below 2%. Comparing the recent progress to the required growth for meeting the 2020 target, it appears that some countries largely exceed the interim targets of the RES Directive 2009/28/EC. Despite this, Europe will need additional policy effort to reach the 2020 target. Critical success factors include implementing effective and efficient policies that attract sufficient investments, reducing administrative and grid related barriers, especially in currently less advanced countries, upgrading the power grid infrastructure, dismantling financial barriers in the heat sector, realising sustainability standards for biomass, and lowering energy demand through increased energy efficiency efforts.     

Frost growth and densification in laminar flow over flat surfaces

One-dimensional frost growth and densification in laminar flow over flat surfaces has been theoretically investigated. Improved representations of frost density and effective thermal conductivity applicable to a wide range of frost circumstances have been incorporated. The validity of the proposed model considering heat and mass diffusion in the frost layer is tested by a comparison of the predictions with data from various investigators for frost parameters including frost thickness, frost surface temperature, frost density and heat flux. The test conditions cover a range of wall temperature, air humidity ratio, air velocity, and air temperature, and the effect of these variables on the frost parameters has been exemplified. Satisfactory agreement is achieved between the model predictions and the various test data considered. The prevailing uncertainties concerning the role of air velocity and air temperature on frost development have been elucidated. It is concluded that that for flat surfaces increases in air velocity have no appreciable effect on frost thickness but contribute to significant frost densification, while increase in air temperatures results in a slight increase the frost thickness and appreciable frost densification.     

Efficient design of orthonormal wavelet bases for signal representation

The efficient representation of a signal as a linear combination of elementary "atoms" or building blocks is central to much signal processing theory and many applications. Wavelets provide a powerful, flexible, and efficiently implementable class of such atoms. In this paper, we develop an efficient method for selecting an orthonormal wavelet that is matched to a given signal in the sense that the squared error between the signal and some finite resolution wavelet representation of it is minimized. Since the squared error is not an explicit function of the design parameters, some form of approximation of this objective is required if conventional optimization techniques are to be used. Previous approximations have resulted in nonconvex optimization problems, which require delicate management of local minima. In this paper, we employ an approximation that results in a design problem that can be transformed into a convex optimization problem and efficiently solved. Constraints on the smoothness of the wavelet can be efficiently incorporated into the design. We show that the error incurred in our approximation is bounded by a function that decays to zero as the number of vanishing moments of the wavelet grows. In our examples, we demonstrate that our method provides wavelet bases that yield substantially better performance than members of standard wavelet families and are competitive with those designed by more intricate nonconvex optimization methods.     

Self‐Compensation in Transparent Conducting F‐Doped SnO2

The factors limiting the conductivity of fluorine‐doped tin dioxide (FTO) produced via atmospheric pressure chemical vapor deposition are investigated. Modeling of the transport properties indicates that the measured Hall effect mobilities are far below the theoretical ionized impurity scattering limit. Significant compensation of donors by acceptors is present with a compensation ratio of 0.5, indicating that for every two donors there is approximately one acceptor. Hybrid density functional theory calculations of defect and impurity formation energies indicate the most probable acceptor‐type defects. The fluorine interstitial defect has the lowest formation energy in the degenerate regime of FTO. Fluorine interstitials act as singly charged acceptors at the high Fermi levels corresponding to degenerately n‐type films. X‐ray photoemission spectroscopy of the fluorine impurities is consistent with the presence of substitutional F_O donors and interstitial F_i in a roughly 2:1 ratio in agreement with the compensation ratio indicated by the transport modeling. Quantitative analysis through Hall effect, X‐ray photoemission spectroscopy, and calibrated secondary ion mass spectrometry further supports the presence of compensating fluorine‐related defects.     

Universal intervention effects on substance use among young adults mediated by delayed adolescent substance initiation.

In this article, the authors examine whether delayed substance initiation during adolescence, achieved through universal family-focused interventions conducted in middle school, can reduce problematic substance use during young adulthood. Sixth-grade students enrolled in 33 rural midwestern schools and their families were randomly assigned to 3 experimental conditions. Self-report questionnaires provided data at 7 time points for the Iowa Strengthening Families Program (ISFP), Preparing for the Drug Free Years (PDFY), and control groups through young adulthood. Five young adult substance frequency measures (drunkenness, alcohol-related problems, cigarettes, illicit drugs, and polysubstance use) were modeled as distal outcomes affected by the average level and rate of increase in substance initiation across the adolescent years in latent growth curve analyses. Results show that the models fit the data and that they were robust across outcomes and interventions, with more robust effects found for ISFP. The addition of direct intervention effects on young adult outcomes was not supported, suggesting long-term effects were primarily indirect. Relative reduction rates were calculated to quantify intervention-control differences on the estimated proportion of young adults indicating problematic substance use; they ranged from 19% to 31% for ISFP and from 9% to 16% for PDFY. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Not so black and white: Memory for ambiguous group members.

Exponential increases in multiracial identities, expected over the next century, create a conundrum for perceivers accustomed to classifying people as their own- or other-race. The current research examines how perceivers resolve this dilemma with regard to the own-race bias. The authors hypothesized that perceivers are not motivated to include ambiguous-race individuals in the in-group and therefore have some difficulty remembering these individuals. Both racially ambiguous and other-race faces were misremembered more often than own-race faces (Study 1), though memory for ambiguous faces was improved among perceivers motivated to include biracial individuals in the in-group (Study 2). Racial labels assigned to racially ambiguous faces determined memory for these faces, suggesting that uncertainty provides the motivational context for discounting ambiguous faces in memory (Study 3). Finally, an inclusion motivation fostered cognitive associations between racially ambiguous faces and the in-group. Moreover, the extent to which perceivers associated racially ambiguous faces with the in-group predicted memory for ambiguous faces and accounted for the impact of motivation on memory (Study 4). Thus, memory for biracial individuals seems to involve a flexible person construal process shaped by motivational factors. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

On codeword design in metric DNA spaces

Finding a large set of single DNA strands that do not crosshybridize to themselves and/or to their complements is an important problem in DNA computing, self-assembly, and DNA memories. We describe a theoretical framework to analyze this problem, gauge its computational difficulty, and provide nearly optimal solutions. In this framework, codeword design is reduced to finding large sets of strands maximaly separated in a DNA space and the size of such sets depends on the geometry of these metric spaces. We show that codeword design is NP-complete using any single reasonable measure that approximates the Gibbs energy, thus practically excluding the possibility of finding any procedure to find maximal sets efficiently. Second, we extend a technique known as shuffling to provide a construction that yields provably nearly-maximal codes. Third, we propose a filtering process that removes strands creating pairs with low Gibbs energies, as approximated by the nearest-neighbor model. These two steps produce large codes of thermodynamic high quality. The proposed framework can be used to gain an understanding of the Gibbs energy landscapes for DNA strands on which much of DNA computing and self-assembly are based.