Quantifying adherence of oxide scales on steels exposed to high temperature and pressure steam

Oxide scale exfoliation is a major concern in fossil fuel power generation because it can cause tube blockages and erode valves and steam turbine components downstream. There is still considerable scientific and commercial interest to improve the mechanistic understanding of oxide failures by developing models to predict exfoliation and the extent of tube blockage as a function of operating conditions and component geometries. Tensile testing inside a scanning electron microscope was conducted on ferritic–martensitic and austenitic steel specimens with the steam side (Fe,Cr)-rich oxides grown after exposures for up to 1000 h in steam with ~100 ppb O₂ at 276 bar and 550°C. Multiple oxide layer cracks and delamination events were observed and analyzed in detail during the tests. Results from the testing agreed well with earlier observations that had identified the failure location at the outer–inner oxide layer for all tested materials. Calculated adhesion energies identified the outer–inner oxide interface of alloy 347HFG as the weakest interface.     

Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors

Recent evidence suggests that fibrotic liver injury in patients with chronic hepatitis C correlates with cellular senescence in damaged liver tissue. However, it is still unclear how senescence can affect replication of the hepatitis C virus (HCV). In this work, we report that an inhibitor of cyclin-dependent kinases 4/6, palbociclib, not only induced in hepatoma cells a pre-senescent cellular phenotype, including G1 arrest in the cell cycle, but also accelerated viral replicon multiplication. Importantly, suppression of HCV replication by direct acting antivirals (DAAs) was barely affected by pre-senescence induction, and vice versa, the antiviral activities of host-targeting agents (HTAs), such as inhibitors of human histone deacetylases (HDACi), produced a wide range of reactions—from a dramatic reduction to a noticeable increase. It is very likely that under conditions of the G1 arrest in the cell cycle, HDACi exhibit their actual antiviral potency, since their inherent anticancer activity that complicates the interpretation of test results is minimized.     

hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination

The recessive form of dystrophic epidermolysis bullosa (RDEB) is a crippling disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Using ectopic expression of hTERT/hTERT + BMI-1 in primary cells, we developed expansible cultures of RDEB fibroblasts and keratinocytes. We showed that they display the properties of their founders, including morphology, contraction ability and expression of the respective specific markers including reduced secretion of type VII collagen (C7). The immortalized keratinocytes retained normal stratification in 3D skin equivalents. The comparison of secreted protein patterns from immortalized RDEB and healthy keratinocytes revealed the differences in the contents of the extracellular matrix that were earlier observed specifically for RDEB. We demonstrated the possibility to reverse the genotype of immortalized cells to the state closer to the progenitors by the Cre-dependent hTERT switch off. Increased β-galactosidase activity and reduced proliferation of fibroblasts were shown after splitting out of transgenes. We anticipate our cell lines to be tractable models for studying RDEB from the level of single-cell changes to the evaluation of 3D skin equivalents. Our approach permits the creation of standardized and expandable models of RDEB that can be compared with the models based on primary cell cultures.     

Exploration of Electrochemical Reactions at Organic–Inorganic Halide Perovskite Interfaces via Machine Learning in In Situ Time‐of‐Flight Secondary Ion Mass Spectrometry

The instability of hybrid organic–inorganic perovskite (HOIP) devices is one of the significant challenges preventing commercialization. Exploring these phenomena is severely limited by the complexity of the intrinsic electrochemistry of HOIPs, the presence of multiple volatile and mobile ionic species, and the possible role of environmentally induced reactions at surfaces and triple‐phase junctions. Here, in situ studies of the electrochemistry of methylammonium lead bromide perovskite with the Au electrode interface are reported via light‐ and voltage‐dependent time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) imaging of lateral perovskite heterostructures. While ToF‐SIMS allows for the visualization of the chemical composition along the surface and its evolution with light and electrical bias, the interpretation of the multidimensional data obtained is often limited due to strong correlations between chemical signatures and the need to track multiple peaks at once. Here, a machine learning workflow combining the Hough transform and non‐negative matrix factorization and non‐negative tensor decomposition is developed to avoid this limitation and extract salient features of associated chemical changes and to separate the light‐ and voltage‐dependent dynamics. Combining these in situ characterizations and the machine learning workflow provides comprehensive information on the chemical nature of moving species, ion accumulation, and interfacial electrochemical reactions in HOIP devices.     

继电器触点回跳消除电路

半导体技术的进展已使集成电路(IC)能够取代很多机械式继电器,但在任意极性的高电压大电流电路中,继电器仍然占据主导地位.然而,这类继电器的触点回跳会给下游电路带来麻烦.解决触点回跳的一种办法是把继电器与一个热插拔控制器结合起来.     

遥控钥匙门禁系统的路径损耗

在遥控钥匙门禁(RKE)系统中,可以用钥匙扣上的发射器从远端开锁,发射器将无线编码发送到汽车内的接收机。遥控钥匙门禁(RKE)系统通常工作在ISM频段,包括315MHz和433．92MHz。随着远程启动和带校验的RKE的出现,设计     

利用高性能同时采样ADC降低高级电力线监测系统的成本

人们对电力需求的增长以及节能意识的增强使得全球在电力传输基础架构领域的投资迅速增长,这也带动了高级电力线监控系统在单相和多相应用中的市场,使其成为新一代智能电网的关键技术。随着对系统性能的要求越来越严格,设计人员开始在电力线监测或多通道SCADA(管理控制和数据采集)系统中关注同时采样、多通道、高性能ADC的使用。     

On systems with shared resources and optimal switching strategies

Simple series systems of identical components with spare parts are considered. It is shown that the cumulative distribution function of a system failure time tends to be a step function as the number of components increases and resources can be shared. An example of ‘continuous resources’ is also described. The time-sharing strategy for standby systems is investigated. It is proved that an optimal rule for a system of standby components with increasing failure rates is the single switching performed at a=t/2, where t is the mission time.     

Three-layer flow membrane system on a microchip for investigation of molecular transport

A stable three-layer flow system, water/organic solvent/water, has been successfully applied for the first time in a microchannel to get rapid transport through an organic liquid membrane. In the continuous laminar flow region, the analyte (methyl red) was rapidly extracted across the microchannel from the donor to the acceptor phase through the organic solvent phase (cyclohexane). Thermal lens microscopy was used to monitor the process. The thickness of the organic phase, sandwiched by the two aqueous phases, was approximately 64 microm, and it was considered as a thin liquid organic membrane. Permeability studies showed the effects of molecular diffusion, layer thickness, and organic solvent-water partition coefficient on the molecular transport. In the microchip, complete equilibration was achieved in several seconds, in contrast to a conventionally used apparatus, where it takes tens of minutes. The thickness of the organic and aqueous boundary layers was defined as equal to the microchannel dimensions, and the organic solvent-water partition coefficient was determined on a microchip using the liquid/liquid extraction system. Experimental data on molecular transport across the organic membrane were in agreement with the calculated permeability based on the three-compartment water/organic solvent/water model. This kind of experiment can be performed only in a microspace, and the system can be considered as a potential biological membrane for future in vitro study of drug transport.     

Approximation algorithms for shop scheduling problems with minsum objective: A correction

We present a correction to the paper, “Approximation algorithms for shop scheduling problems with minsum objective” (Journal of Scheduling 2002; 5:287–305) by Queyranne and Sviridenko. This correction provides a correct derivation of its 2eρ approximation result. Wenhua Li and Jinjiang Yuan: Project supported by NNSFC (Grant 10371112) and NSFHN (Grant 0411011200). Maurice Queyranne: Supported by research grants from NSERC, the Natural Sciences and Engineering Research Council of Canada.