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971.
MV García R Hernández-Berciano C López-Mediavilla A Orfao JM Medina 《Canadian Metallurgical Quarterly》1997,237(2):403-409
The effect of adrenaline on the control of respiratory activity of mitochondria from fetal hepatocytes in primary culture was studied. In the absence of adrenaline, the respiratory control ratio (RCR) of mitochondria increased during the first 3 days of culture due to a decrease in the rate of state 4 respiration. The presence of adrenaline in the incubation medium further increased the mitochondrial RCR through a decrease in the rate of respiration in state 4 and to an increase in the respiration rate in state 3. The effect of adrenaline was mimicked by dibutyryl-cAMP, forskolin, and isobutyl methyl xanthine. All these compounds increased cAMP concentrations, suggesting that cAMP may be involved in the effect of adrenaline. The increase in intracellular free Ca2+ concentrations caused by phenylephrine, vasopressin, or thapsigargin was also accompanied by an increase in the RCR, suggesting that both phenomena are associated. Dibutyryl-cAMP also increased free Ca2+ concentrations, suggesting that the effects of cAMP may be mediated by free Ca2+ concentrations. Adrenaline, dibutyryl-cAMP, phenylephrine, vasopressin, and thapsigargin promoted adenine nucleotide accumulation in mitochondria; this may be an intermediate step in the activation of mitochondrial respiratory function. These results suggest that the stimulatory effect of adrenaline on mitochondrial maturation in cultured fetal rat hepatocytes may be exerted through a mechanism in which both cAMP and Ca2+ act as second messengers. It is concluded that the effect of adrenaline on mitochondrial maturation is exerted by both alpha- and beta-adrenergic mechanisms and is mediated by the increase in adenine nucleotide contents of mitochondria. 相似文献
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974.
We investigated in vivo expression of myosin heavy chain (MHC) isoforms, 17 kDa myosin light chain (MLC17), and phosphorylation of the 20 kDa MLC (MLC20) as well as mechanical performance of chemically skinned fibers of normal and hypertrophied smooth muscle (SM) of human myometrium. According to their immunological reactivity, we identified three MHC isoenzymes in the human myometrium: two SM-MHC (SM1 with 204 kDa and SM2 with 200 kDa), and one non-muscle specific MHC (NM with 196 kDa). No cross-reactivity was detected with an antibody raised against a peptide corresponding to a seven amino acid insert at the 25K/50K junction of the myosin head (a-25K/50K) in both normal and hypertrophied myometrium. In contrast, SM-MHC of human myomatous tissue strongly reacted with a-25K/50K. Expression of SM1/SM2/NM (%) in normal myometrium was 31.7/34.7/33.6 and 35.1/40.9/24 in hypertrophied myometrium. The increased SM2 and decreased NM expression in the hypertrophied state was statistically significant (P < 0.05). MHC isoform distribution in myomatous tissue was similar to normal myometrium (36.3/35.3/29.4). In vivo expression of MLC17a increased from 25.5% in normal to 44.2% in hypertrophied (P < 0.001) myometrium. Phosphorylation levels of MLC20 upon maximal Ca(2+)-calmodulin activation of skinned myometrial fibers were the same in normal and hypertrophied myometrial fibers. Maximal force of isometric contraction of skinned fibers (pCa 4.5, slack-length) was 2.85 mN/mm2 and 5.6 mN/mm2 in the normal and hypertrophied state, respectively (P < 0.001). Apparent maximal shortening velocity (Vmax(appt), extrapolated from the force-velocity relation) of myometrium rose from 0.13 muscle length s-1 (ML/s) in normal to 0.24 ML/s in hypertrophied fibers (P < 0.001). 相似文献
975.
Raimundo Bamb Naya Pablo de la Cal Nicol s Carmen D ez Medina Isabel Ezquerra Sergio Garc a-P rez Javier Moncl s 《建筑学研究前沿(英文版)》2023,12(3):477-495
The starting point of this research is the urban model promoted by the United Nations through the Sustainable Development Goals (SDGs). The importance of public spaces is especially highlighted in Goal 11.7: “provide universal access to safe, inclusive, and accessible, green, and public spaces.” The quality of public spaces can be properly assessed by reconsidering the quality indicators and investigating their development potential, which is the main focus of our research. This study is based on a theoretical and methodological reflection on the quality of public space, considering the contributions of some contemporary urban traditions and a few recent experiences. From this perspective, a variety of public space projects developed in the European sphere have been selected and analyzed. The quality parameters identified in this initial analysis were then applied to the analysis of nine representative case studies in three Spanish cities (Madrid, Barcelona, and Zaragoza). The main goal is to propose a methodology that helps to evaluate the quality of those public spaces and determine its relationship with the safety, accessibility, and inclusiveness conditions they offer. 相似文献
976.
D. Flores-Medina Nelson Flores Medina F. Hernández-Olivares 《Materials and Structures》2014,47(7):1185-1193
The following research is focused on establishing the differences in the re-use as aggregate in dry consistency concretes of two types of rubber obtained in the process of Tyre recycling, recycled rubber from tyres (RRT): granulated sizes (4–8 mm) of high quality recycled rubber (HQRR) and the waste of the recycling process: steel and textile fibers with rubber tracks (waste from recycled rubber, WRR). Both types were classified and added as aggregate in substitution of coarse aggregates from 20 to 100 % by volume. The physical and mechanical behavior of WRR in concretes was compared with reference concrete and series with HQRR for a future use in precast concrete pieces. In both samples a reduction of mechanical resistance occurs in proportion with the amounts of rubber of substitution, but less in serials with WRR with a successful combination of steel and textile fiber. WRR shows furthermore a reduction in properties such as workability and density, but also an increment in porosity. These facts facilitate new options for waste from RRT in concretes and therefore lower energy costs, achieving a success rate in the recycling process close to 100 %. 相似文献
977.
Mellisho CD González-Barrio R Ferreres F Ortuño MF Conejero W Torrecillas A García-Mina JM Medina S Gil-Izquierdo A 《Journal of the science of food and agriculture》2011,91(12):2132-2139
BACKGROUND This study was designed to describe the phenolic status of lemon juice obtained from fruits of lemon trees differing in iron (Fe) nutritional status. Three types of Fe(III) compound were used in the experiment, namely a synthetic chelate and two complexes derived from natural polymers of humic and lignine nature. RESULTS All three Fe(III) compounds were able to improve the Fe nutritional status of lemon trees, though to different degrees. This Fe(III) compound effect led to changes in the polyphenol content of lemon juice. Total phenolics were decreased (~33% average decrease) and, in particular, flavanones, flavones and flavonols were affected similarly. CONCLUSION Iron‐deficient trees showed higher phenolic contents than Fe(III) compound‐treated trees, though Fe deficiency had negative effects on the yield and visual quality of fruits. However, from a human nutritional point of view and owing to the health‐beneficial properties of their bioavailable phenolic compounds, the nutritional quality of fruits of Fe‐deficient lemon trees in terms of phenolics was higher than that of fruits of Fe(III) compound‐treated lemon trees. Moreover, diosmetin‐6,8‐di‐C‐glucoside in lemon juice can be used as a marker for correction of Fe deficiency in lemon trees. Copyright © 2011 Society of Chemical Industry 相似文献
978.
This study examined the effectiveness of 3 different training types on commercial motor vehicle (CMV) drivers’ skill levels. The training types included a conventional 8-week certified course, a conventional 8-week certified course with approximately 60% of driving time spent in a CMV driving simulator, and a Commercial Driver's License (CDL) test focused short course. Participants’ scores on the Division of Motor Vehicles (DMV) road and range tests were assessed. In addition to their DMV scores, participants replicated DMV road and range driving tests in an instrumented vehicle and the CMV driving simulator. Results indicated no training group differences in DMV road tests. There were differences between training groups on DMV range tests and real truck and simulator versions of the DMV road and range tests; on these tests conventional- and simulator-trained participants generally scored higher than CDL-focused participants. However, all groups performed higher in the real truck than in the simulator for both road and range tests. These findings indicate the need for a minimum standard of entry-level CMV driver training as well as support of the use of a driving simulator for training entry-level drivers; however, testing using a simulator does not appear to be feasible with current technology. 相似文献
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980.