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21.
Proteases involved in mammary tissue damage during endotoxin-induced mastitis in dairy cows 总被引:4,自引:0,他引:4
Mehrzad J Desrosiers C Lauzon K Robitaille G Zhao X Lacasse P 《Journal of dairy science》2005,88(1):211-222
During and after diapedesis, milk polymorphonu-clear neutrophils (PMN) release many proteases that have the potential of degrading extracellular matrix proteins and milk proteins. However, the kinetics of milk proteolysis during inflammation and the underlying mechanisms are poorly defined. The enzymes involved in bovine mammary tissue destruction were investigated in this study using an endotoxin-induced mastitis model. Using zymography techniques, the proteolytic activity of milk and mammary tissue during mastitis was examined. Mastitic milk produced 6 caseolysis bands, 4 of which differed from the ones produced by plasmin. Peak proteolytic activity, bovine serum albumin contents, and mammary tissue damage occurred between 6 and 12 h postchallenge. Mastitic milk proteases hydrolyzed casein, gelatin, collagen, hemoglobin, mammary gland membrane proteins, and lactoferrin. These results confirm that mastitic milk proteases have a broad spectrum of activity. The hydrolytic activity of mastitic milk was partially inhibited by aprotinin, EDTA, 1,10-phenanthroline, leupeptin, and pefabloc. When cocultured with normal mammary tissue, mastitic milk, but not normal milk, caused mammary tissue degradation. In situ zymography of mammary gland showed increased proteolytic activity in mastitic tissue compared with normal tissue. The similarity of zymograms of mastitic milk, blood PMN, milk somatic cells, and PMN strongly suggests that proteases in mastitic milk mainly originate from milk PMN. These results suggest that proteases released by PMN are actively involved in udder tissue damage during mastitis. 相似文献
22.
Static mixer (SM) can be applied for emulsification, but the fundamental understanding of the nature of fluid flow and mixing in static mixers, is however poor. Droplet size is a very important parameter in miniemulsion systems and affects strongly the mechanism of particle formation in polymerization reactions. In this study, static mixer was used as homogenization device for emulsification of methyl methacrylate (MMA). Re number (Re) was obtained for SM inserted tube in different flow rates. It was demonstrated the nature of fluid flow was turbulent under our experimental conditions. The relationship between droplet size—the most important variable in our study—and Weber number (We) was investigated. The results showed that the ratio of the droplet size to the pipe diameter was fit as an exponential function with an order of −0.35. The polymerization of created droplets under certain We values by SM showed that it is possible to obtain a reasonable 1 : 1 copy of droplets to the particles. All these, indicate that using relationship between We and droplet size allow one to obtain acceptable condition of droplet nucleation in miniemulsion polymerization. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献
23.
24.
Mehrzad Taherzadehboroujeni Roozbeh Kalhor Gregory B. Fahs Robert B. Moore Scott W. Case 《Polymer Engineering and Science》2020,60(5):879-888
The ability to quickly develop predictions of the service lifetime of plastic pipes at different load levels allows designers to choose the best plastic material and design pipe for a specific application. Additionally, it helps material producers to rapidly design, manufacture, test, screen, and modify the base polymeric material. The aim of this study is to introduce a combined experimental and analytical framework to develop accelerated lifetime estimates for semi-crystalline plastic pipes which is sensitive to the structure, orientation, and morphology changes introduced by changing processing conditions. To accomplish this task, high density polyethylene (HDPE) is chosen as the exemplary base material and custom fixtures are developed to admit tensile and hoop burst tests on the as-manufactured HDPE pipes. A pressure-modified Eyring flow equation is employed to predict the rupture lifetime of HDPE pipes using the measured mechanical properties under uniaxial tensile and compression loading in different temperatures and strain rates. The method allows the prediction of pipe service lifetimes in excess of 50 years using experiments conducted over approximately 10 days instead of the traditional 13 months. POLYM. ENG. SCI., 60:879–888, 2020. © 2019 Society of Plastics Engineers 相似文献
25.
Knowing of thermoset curing kinetics is essential for process development, quality control, and achieving desirable products. Hence, in this article, cure kinetics of an EPON 828 epoxy resin/dicyandiamide curing agent/diuron accelerator system is investigated. This resin system is usually used for the production of epoxy/glass fiber prepregs used in wind turbine blades. For this, differential scanning calorimetry analysis is used and the effect of temperature, weight percentage, and size of nanosilica is studied by conducting isothermal tests at several temperatures for samples with and without nanoparticles. An autocatalytic curing model is applied to describe the cure kinetic of system and then the variations in model parameters calculated by curve fitting using the MATLAB software. The results show that the increase in temperature, weight percentage of nanosilica from 0 to 6%, and surface area of nanosilica particles lead to the increase in curing rate, whereas the increase in the percentage and surface area of nanosilica particles significantly decreases total heat of reaction. At the end, the relation between each of model parameters and the total surface area of nanosilica particles, calculated by mathematical equations, is obtained. The allowable maximum surface area of nanosilica used in the mathematical equations is 12 m2 g−1. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47958. 相似文献
26.
Mehrzad Sharifian Mehrdad Sharifian Mehrnaz Sharifian 《International Journal of Mechanics and Materials in Design》2018,14(3):329-344
When structures undergo extreme loading conditions, the materials pass the elastic limits. Therefore, to preserve economy as well as safety, it is essential to perform a realistic elastoplastic analysis using the constitutive equations in plasticity. On the other hand, computing the stress alongside its associated variables on Gauss points is a delicate process and virtually the most important part of these analyses. In this study, an efficient stress-updating technique is presented for the constitutive rate equations of the pressure sensitive materials such as concrete, rock, soil and some kind of metals. Accordingly, the Drucker–Prager plasticity is utilized to consider the hydrostatic pressure in addition to the J 2-invariant of the deviatoric stress. Moreover, the isotropic and kinematic hardenings are used to take into account more realistic behavior of the materials. Finally, a wide range of numerical tests is carried out to show the performance of the presented method together with the application of the suggested formulations in elastoplastic analysis of a gravity dam. 相似文献
27.
Using concurrence as a measure of entanglement, we present analytical and numerical study of entanglement dynamics in a two-qutrit system in the presence of the Dzyaloshinskii–Moriya interaction, as a function of the parameters involved. Three distinct initial states: a superposition of the ground and the first excited state, the Bell-type state and a superposition of qutrit coherent states will be considered in this investigation. 相似文献
28.
Works pertinent to arterial transport models are analyzed and a critical assessment of the models utilized in the study of fluid flow and mass transfer within the arteries is presented with an emphasis on the role of porous media. Arterial transport models are assessed and classified based on their ability to physically prescribe the arterial anatomy as well as the related transport processes. Pertinent models such as wall-free, homogeneous-wall, and multi-layer models as well as the governing equations and different types of boundary conditions utilized in each model are analyzed. 相似文献
29.
Hossein Salehi Vaziri Iraj Amiri Omaraei Mohamadreza Abadyan Mehrzad Mortezaei Nariman Yousefi 《Materials & Design》2011,32(8-9):4537-4542
In this work, solvent blending in combination with extruding are applied to provide polystyrene/silica nanocomposite specimens. Transmission electron microscope (TEM) and scanning electron microscope (SEM) show same nanoparticle dispersion in PS matrix in low to high filler loadings. Differential scanning calorimetry (DSC), dynamic mechanical thermal analyzer (DMTA) and thermogravimetric analyzer (TGA) were used to study the thermophysical characteristic of the nanocomposites in solid state. In addition, the melt state rheological behavior of the samples was investigated under constant and zero shear rates. Interestingly, different behaviors were detected in nanocomposites in low and high nanoparticle loadings. In addition, rheological characteristics of molten polymer are dramatically affected in samples with low nanosilica concentration while stabilized in high filler loadings. 相似文献
30.
Muhammad Reza Saffarian Mohammad Hossein Hamedi Mehrzad Shams 《Korean Journal of Chemical Engineering》2010,27(5):1497-1508
An axisymmetric single-phase model that predicts the sedimentation of activated sludge in a circular secondaryclarifier is
developed. The k-ɛ turbulence model is used on a two-dimensional, orthogonal and stepwise grid. The concentration equation, which is extended
to incorporate the sedimentation of activated sludge in the field of gravity, governs the mass transfer in the clarifier.
The computational domain includes the sludge blanket where the viscosity is affected by the rheological behavior of the sludge.
Results in case of non-Newtonian fluid model are compared with another numerical approach provided by Lakehal et al. Non-Newtonian
fluid models—Bingham, Casson, and Herschel-Bulkley—are used. The influence of settling velocity functions and non-Newtonian
models on the flow behavior isinvestigated. Finally, the best models are introduced and the ways that the non-Newtonian model
introduces the plastic viscosity are discussed. 相似文献