Determination of the scattering coefficient and the anisotropy factor from laser Doppler spectra of liquids including blood

Laser Doppler measurements were performed on scattering liquids flowing through a highly scattering static medium to determine the scattering coefficient and the anisotropy factor of the liquids. Monte Carlo simulations of light propagation in the static and moving media were used to calculate the Doppler spectra for suspensions of polystyrene spheres in water, and these spectra were in excellent agreement with experimental results. A faster Monte Carlo code was developed so that nonlinear regressions to the measured laser Doppler spectra could be used to determine the anisotropy factor of other liquids. This approach was used to deduce the scattering properties of Intralipid and blood at λ = 820 nm. It was found that the anisotropy factor of blood is well described by Mie theory in contradiction to results reported in the literature that were obtained by goniometric measurement of the phase function.     

Combined hyperlipidemia in transgenic mice overexpressing human apolipoprotein Cl

We have generated transgenic mice over-expressing human apolipoprotein CI (apo CI) using the native gene joined to the downstream 154-bp liver-specific enhancer that we defined for apo E. Human apo CI (HuCI)-transgenic mice showed elevation of plasma triglycerides (mg/dl) compared to controls in both the fasted (211 +/- 81 vs 123 +/- 52, P = 0.0001) and fed (265 +/- 105 vs 146 +/- 68, P < 0.0001) states. Unlike the human apo CII (HuCII)- and apo CIII (HuCIII)-transgenic mouse models of hypertriglyceridemia, plasma cholesterol was disproportionately elevated (95 +/- 23 vs 73 +/- 23, P = 0.002, fasted and 90 +/- 24 vs 61 +/- 14, P < 0.0001, fed). Lipoprotein fractionation showed increased VLDL and IDL + LDL with an increased cholesterol/triglyceride ratio (0.114 vs 0.065, P = 0.02, in VLDL). The VLDL apo E/apo B ratio was decreased 3.4-fold (P = 0.05) and apo CII and apo CIII decreased in proportion to apo E. Triglyceride and apo B production rates were normal, but clearance rates of VLDL triglycerides and postlipolysis lipoprotein "remnants" were significantly slowed. Plasma apo B was significantly elevated. Unlike HuCII- and HuCIII-transgenic mice, VLDL from HuCI transgenic mice bound heparin-Sepharose, a model for cell-surface glycosaminoglycans, normally. In summary, apo CI overexpression is associated with decreased particulate uptake of apo B-containing lipoproteins, leading to increased levels of several potentially atherogenic species, including cholesterol-enriched VLDL, IDL, and LDL.     

eSeeTrack--visualizing sequential fixation patterns

We introduce eSeeTrack, an eye-tracking visualization prototype that facilitates exploration and comparison of sequential gaze orderings in a static or a dynamic scene. It extends current eye-tracking data visualizations by extracting patterns of sequential gaze orderings, displaying these patterns in a way that does not depend on the number of fixations on a scene, and enabling users to compare patterns from two or more sets of eye-gaze data. Extracting such patterns was very difficult with previous visualization techniques. eSeeTrack combines a timeline and a tree-structured visual representation to embody three aspects of eye-tracking data that users are interested in: duration, frequency and orderings of fixations. We demonstrate the usefulness of eSeeTrack via two case studies on surgical simulation and retail store chain data. We found that eSeeTrack allows ordering of fixations to be rapidly queried, explored and compared. Furthermore, our tool provides an effective and efficient mechanism to determine pattern outliers. This approach can be effective for behavior analysis in a variety of domains that are described at the end of this paper.     

Evaluating and improving the content access in KAD

We analyze in detail the content retrieval process in kad. kad implements content search (publish and retrieval) functions that use the Kademlia Distributed Hash Table for content routing. Node churn is quite common in peer-to-peer systems and results in information loss and stale routing table entries. To deal with node churn, kad issues parallel route requests and publishes multiple redundant copies of each piece of information. We identify the key design parameters in kad and present an analytical model to evaluate the impact of changes in the values of these parameters on the overall lookup latency and message overhead. Extensive measurements of the lookup performance using an instrumented client allow us to validate the model. The overall lookup latency is in most cases 5 s or larger. We elucidate the cause for such high lookup latencies and propose an improved scheme that significantly decreases the overall lookup latency without increasing the overhead.     

Mitochondria: A mirror into cellular dysfunction in heart disease

Cardiovascular (CV) disease is the single most significant cause of morbidity and mortality worldwide. The emerging global impact of CV disease means that the goals of early diagnosis and a wider range of treatment options are now increasingly pertinent. As such, there is a greater need to understand the molecular mechanisms involved and potential targets for intervention. Mitochondrial function is important for physiological maintenance of the cell, and when this function is altered, the cell can begin to suffer. Given the broad range and significant impacts of the cellular processes regulated by the mitochondria, it becomes important to understand the roles of the proteins associated with this organelle. Proteomic investigations of the mitochondria are hampered by the intrinsic properties of the organelle, including hydrophobic mitochondrial membranes; high proportion of basic proteins (pI greater than 8.0); and the relative dynamic range issues of the mitochondria. For these reasons, many proteomic studies investigate the mitochondria as a discrete subproteome. Once this has been achieved, the alterations that result in functional changes with CV disease can be observed. Those alterations that lead to changes in mitochondrial function, signaling and morphology, which have significant implications for the cardiomyocyte in the development of CV disease, are discussed.     

Trench-Hall devices

A trench-Hall device sensitive to magnetic inductions parallel to the chip surface is reported in this paper. The vertically oriented active region is defined by two parallel trenches with a distance of only 2.4 μm. Deep contacts connect the active region of the device at its bottom at a depth of 20 μm. These deep contacts allow a symmetrical operating condition of the active region analogous to a lateral symmetrical Hall plate, which is favorable for dynamic offset reduction. With the presented technology, trench-Hall devices with a sensitivity of 320 V/A with a nonlinearity below 0.1% are realized. Additionally, the presented fabrication technique enables the electrically insulated cointegration of sensor and circuitry on a single CMOS chip     

A Longitudinal Examination of Causal Attributions and Depression Symptomatology in Rheumatoid Arthritis.

Objective: Examine longitudinal relationships between causal attributions and depression symptoms in adults with rheumatoid arthritis (RA). Study Design: Cross-lagged panel correlations tested the temporal precedence of attributions relative to depression symptoms over 1 year. Participants: Forty-two participants completed self-report instruments on 2 occasions. Main Outcome Measures: The Inventory to Diagnose Depression and the Attributional Style Questionnaire. Results: Time 1 attributions predicted increased levels of depression symptoms at Time 2 after perceived pain and disability were controlled: Time 1 depression symptoms were unrelated to Time 2 attributions. Cross-lagged correlation comparisons revealed statistical dominance for attribution-depression relationships relative to depression-attribution relationships. Conclusions: Results support cognitive diathesis conceptualizations of depression and support cognitive-behavioral treatments for depression in RA. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Internationale Rekrutierung und Migration von Ärztinnen, Ärzten und Personal in Gesundheitsfachberufen: ein qualitatives Scoping Review der Public Health Literatur

Background

The international recruitment and migration of health workers has long been debated in the health policy literature. This study assesses to what extent the ethics of international recruitment are targeted in existing public health peer-reviewed literature, with a focus on concepts and in-depth consideration within articles. It further critically discusses hidden implications of this discourse regarding equity-creating health policy.

Methods

We conducted a qualitative scoping literature review searching the databases PubMed and Web of Science. Articles were assessed for quality using the Critical Appraisal Skills Program (CASP) checklist for qualitative research. Drawing on a thematic analysis approach, common themes across the sample were identified, coded and analyzed.

Results

A total of 17 articles were included in the analysis after searches and quality appraisal. These articles predominantly targeted nursing professions and physicians, and were written mainly by authors working in the Global North. Thematic analysis revealed that ethics are generally discussed in connection with overviews regarding the situation of health systems as well as regarding inequities between countries. Their focus lies more on migration between countries than between regions within a country. Proposed solutions are usually of an economic nature and range from global financial equalization between countries to national improvements in working conditions. Creative evaluation schemes for these solutions are rarely discussed in depth. Overall, the academic ethics discussion focuses predominantly on the health systems level, and views migration of health workers as problematic.

Conclusions

Our results suggest that academic research operates and aims to inform policy within the current system, without necessarily proposing fundamental changes to the status quo. Thus, an ethics discourse of health workforce recruitment may serve to increase awareness of inequalities without supporting radical change. Further research into intersectional aspects of health worker migration, including gender and ethnicity, may be useful for the discussion on inequities.

     

Gender differences and the effects of perceived internet privacy on self-reports of sexual behavior and sociosexuality

The use of the Internet for conducting research has found that the level of anonymity intrinsic to web-based surveys is useful in deterring various participant biases. Though, concerns about Internet privacy could interfere with the effects of perceived anonymity and influence how a person responds. In this study, participants were asked to take an Internet survey and then were randomly assigned to conditions with varying degrees of implied privacy in which they answered questions about their desire for casual sex (sociosexuality) and their lifetime sexual partners. Only in the condition without any threat to the participant’s anonymity was there no difference between men and women’s reports of their sexual behavior. However, in every condition men reported having a significantly higher sociosexuality than women. Findings are consistent with both the biologic and gender-biased explanations in differences of self-report for sexual information. Implications for social constraints and Internet based research are discussed.     

Impact of enzymatic mash maceration and storage on anthocyanin and color retention of pasteurized strawberry purées

Strawberry purées were prepared using a commercial polygalacturonase (PG) and a highly purified pectinesterase (PE) preparation, respectively. To elucidate the effect of pectin on color stability following enzymatic pulp maceration, pectin composition was studied by isolating and fractionating the alcohol-insoluble residue from the strawberry purées. The purées were stored at +20 and +4 °C in the dark over a period of 24 weeks monitoring the amounts of monomeric and polymeric anthocyanins as well as antioxidant activities (FRAP, TEAC). Individual anthocyanins were analyzed by HPLC–DAD–MS ⁿ , and color measurements were obtained in the CIE L*a*b* system. Pectin composition was significantly modified following enzymatic maceration of the purées. While PG treatment generally resulted in pectin losses, oxalate-soluble pectins were increased in PE-treated purées. After 24 weeks of storage, the best anthocyanin retention was observed in PE-treated purées. Such products also revealed greatest anthocyanin half-life values and lowest proportion of polymeric pigments. Compared to an untreated control, enzymatic purée maceration using the PG was also beneficial for pigment retention, but less effective than PE. In contrast, color and antioxidant activity were independent of both enzymatic treatments. An initial heating step (90 °C, 10 s) for immediate inactivation of native enzymes such as polyphenoloxidases slightly improved pigment stability, while lowered temperature during mash maceration was less effective. However, by far best color and pigment retention were achieved when the purées were stored at 4 °C in the dark.