The Mechanism of Energy Coupling in H+/Na+-Pumping Membrane Pyrophosphatase—Possibilities and Probabilities

Membrane pyrophosphatases (mPPases) found in plant vacuoles and some prokaryotes and protists are ancient cation pumps that couple pyrophosphate hydrolysis with the H⁺ and/or Na⁺ transport out of the cytoplasm. Because this function is reversible, mPPases play a role in maintaining the level of cytoplasmic pyrophosphate, a known regulator of numerous metabolic reactions. mPPases arouse interest because they are among the simplest membrane transporters and have no homologs among known ion pumps. Detailed phylogenetic studies have revealed various subtypes of mPPases and suggested their roles in the evolution of the “sodium” and “proton” bioenergetics. This treatise focuses on the mechanistic aspects of the transport reaction, namely, the coupling step, the role of the chemically produced proton, subunit cooperation, and the relationship between the proton and sodium ion transport. The available data identify H⁺-PPases as the first non-oxidoreductase pump with a “direct-coupling” mechanism, i.e., the transported proton is produced in the coupled chemical reaction. They also support a “billiard” hypothesis, which unifies the H⁺ and Na⁺ transport mechanisms in mPPase and, probably, other transporters.     

Breath figure templated semifluorinated block copolymers with tunable surface properties and binding capabilities

Patterning of functionalized polymeric surfaces enables the adjustment of their characteristics and use in novel applications. We prepared breath figure (BF) films from three semifluorinated diblock copolymers, which all are composed of a polystyrene block and a semifluorinated one to compare their surface properties. “Click” chemistry was employed to one of the polymers, containing a poly(pentafluorostyrene) block to incorporate hydrophilic sugar or carboxylic acid moieties. The structure of the polymer alters the obtained porous morphology of the films. Contact angle (CA) analyses of the BF films reveals that the surface porosity increases water CAs compared with solvent cast films, and, in the case of hydrophobic polymers, leads to significant increase in the CAs of dodecane. The hydrophobicity of the BF films is further amplified by the removal of the topmost layer which leads in some cases to superhydrophobic surfaces. BF films containing glucose units are hydrophilic exhibiting water CAs below 90°. These glycosylated porous surfaces are shown to bind lectin Con A‐FITC or can be labelled with isothiocyanate marker. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41225.     

Radio-wave exposure of the human head: analytical study based on a versatile eccentric spheres model including a brain core and a pair of eyeballs

A versatile eccentric-spheres model of the human head is used in this paper to investigate radio-wave absorption. Numerical results, obtained by use of an exact analytical solution, are presented for the total, percentage, and gram-specific absorption. Interest is mainly in the brain and in the eyes of an adult or an infant head. Our model comprises a host sphere and several spherical inclusions, all concentrically stratified with respect to their own center. Any number of inclusions and any number of concentric layers for the host sphere and each one of the inclusions can be considered. Excitation is provided either by a plane-wave or by a nearby electric dipole. The analytical solution is obtained by use of the indirect-mode matching method. The theory of this paper and the accompanying computer code constitute a versatile tool for analytical studies of cellular-phone interactions with the human head. Specific absorption rate maps in a horizontal cross section of the head model manifest the existence of hot spots in the eyes and near the center of the brain.     

Spatio‐temporal variability in underwater light climate in a turbid river‐floodplain system: Driving factors and estimation using Secchi disc

The underwater light climate has important effects on primary producers. The aim of this research was to evaluate its variability in a turbid river‐floodplain system. Photosynthetically active radiation (PAR) was measured in the Middle Paraná River during different hydrological phases to (a) analyse the photosynthetically active radiation attenuation coefficient (k) and euphotic depth (Z_eu) as well as their associations with optically active components and (b) develop and evaluate indices and regression models based on Secchi disc (SD) measurements to estimate k and Z_eu. Values of k were higher in the fluvial system than in the floodplain and during low‐water stage than high‐water stage. Particulate components controlled the light climate variability. Chromophoric dissolved organic matter and chlorophyll‐a had significant effects during floods. The estimation of k and Z_eu was sensitive to temporal but not to spatial variations. The highest prediction accuracy was observed when using specific non‐linear regressions for each hydrological phase, especially for Z_eu estimation (low stage: k = 1.76 × SD^?0.80, Z_eu = 2.62 × 1/SD^?0.80; high stage: k = 2.04 × SD^?0.53, Z_eu = 2.26 × 1/SD^?0.53). The indices k × SD and Z_eu/SD were significantly different from those proposed for clear water environments. It is concluded that temporal variations should be considered when estimating k and Z_eu in turbid river‐floodplain systems because of the temporal heterogeneity in optically active components. Considering that ecological implication of the light climate depends on Z_eu:depth ratio, we propose to estimate Z_eu instead of k. Finally, indices proposed for clear water environments are not recommended to be applied to turbid environments.     

Chaos arising from the hydrological behaviour of a floodplain river during the last century

The hydrological regime is the main factor governing the functioning of floodplain rivers. A full comprehension of its dynamic leads to a better understanding of the system's behaviour. We analysed the daily water level of the Paraná River during the last century at three gauge stations using linear and non-linear tools to characterise the hydrological dynamic and to analyse to what extent chaotic behaviour prevails. The three water level time series were characterised as non-linear and non-stationary by power spectrum, autocorrelation function, and surrogate test analyses. A strange attractor was developed when the phase space was reconstructed, having a low dimensional chaos supported by correlation dimension, positive maximum Lyapunov exponents, and recurrence quantification analysis. The sample entropy analysis shows that the system resulted unpredictable beyond a temporal threshold, and with an intermediate hydrological complexity, while Hurst exponent characterised it as persistent and with sensitive dependence on initial conditions. In a general overview, all the evidence obtained indicates that the Paraná River's behaviour is at the edge of chaos. A latitudinal gradient of decreasing chaoticity was observed as the floodplain extent increased, whereas complexity was highest at the intermediate river station due to the inflow of tributaries with different hydrology. This paper attempts to offer some additional insights for understanding the hydrological behaviour of floodplain rivers and the most appropriate methods to understand their complexity.     

WCDMA multicarrier receiver for base-station applications

The multicarrier receiver IC described in this paper receives four adjacent WCDMA channels simultaneously in order to reduce the component count of a base-station. The receiver uses low-IF architecture and it is fabricated with a 0.25-/spl mu/m SiGe BiCMOS process to meet the high-performance requirements set by the base-station application. The receiver includes a dual-input low-noise amplifier (LNA), quadrature mixers, a local-oscillator (LO) divider, IIP2 calibration circuits, 10-MHz low-pass filters, and ADC buffers. The receiver noise figures, measured over the downconverted WCDMA channels centered at 2.5-MHz and 7.5-MHz intermediate frequencies, are 3.0 dB and 2.6 dB, respectively. The receiver achieves 47-dB voltage gain and -12-dBm out-of-band IIP3 and consumes 535mW from a 2.5-V supply.     

On the classical shell model underlying bilinear degenerated shell finite elements

We study the shell models arising in the numerical modelling of shells by bilinear degenerated shell finite elements. The numerical model of a cylindrical shell obtained by using flat shell elements is given an equivalent formulation based on a classical two‐dimensional shell model. We use the connection between the models to explain how a parametric error amplification difficulty or locking is avoided by some elements. Copyright © 2001 John Wiley & Sons, Ltd.     

Evaluation of some metals in Brazilian coffees cultivated during the process of conversion from conventional to organic agriculture

The aim of this study was to evaluate the presence of nutrients and toxic elements in coffees cultivated during the process of conversion, on organic agriculture, in southwest Bahia, Brazil. Levels of the nutrients and toxic elements were determined in samples of soils and coffee tissues from two transitional organic farms by atomic absorption spectrometry (FAAS). The metals in soil samples were extracted by Mehlich1 and USEPA-3050 procedures. Coffee samples from both farms presented relatively high levels of Cd, Zn and Cu (0.75, 45.4 and 14.9 μg g⁻¹, respectively), but were still below the limits specified by the Brazilian Food Legislation. The application of statistical methods showed that this finding can be attributed to the addition of high amounts of organic matter during the flowering tree period which can act on the bioavailability of metal ions in soils.     

Catalytic Asymmetry in Homodimeric H+-Pumping Membrane Pyrophosphatase Demonstrated by Non-Hydrolyzable Pyrophosphate Analogs

Membrane-bound inorganic pyrophosphatase (mPPase) resembles the F-ATPase in catalyzing polyphosphate-energized H⁺ and Na⁺ transport across lipid membranes, but differs structurally and mechanistically. Homodimeric mPPase likely uses a “direct coupling” mechanism, in which the proton generated from the water nucleophile at the entrance to the ion conductance channel is transported across the membrane or triggers Na⁺ transport. The structural aspects of this mechanism, including subunit cooperation, are still poorly understood. Using a refined enzyme assay, we examined the inhibition of K⁺-dependent H⁺-transporting mPPase from Desulfitobacterium hafniensee by three non-hydrolyzable PP_i analogs (imidodiphosphate and C-substituted bisphosphonates). The kinetic data demonstrated negative cooperativity in inhibitor binding to two active sites, and reduced active site performance when the inhibitor or substrate occupied the other active site. The nonequivalence of active sites in PP_i hydrolysis in terms of the Michaelis constant vanished at a low (0.1 mM) concentration of Mg²⁺ (essential cofactor). The replacement of K⁺, the second metal cofactor, by Na⁺ increased the substrate and inhibitor binding cooperativity. The detergent-solubilized form of mPPase exhibited similar active site nonequivalence in PP_i hydrolysis. Our findings support the notion that the mPPase mechanism combines Mitchell’s direct coupling with conformational coupling to catalyze cation transport across the membrane.