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111.
A variable friction model that relates the parameters of sheet metal drawing to the local lubrication conditions taking place during the deformation, has been integrated to a finite element program. Variable friction coefficients for the contacting surfaces are determined from the friction model, which uses the related parameters obtained at each time step of the finite element program as inputs. A number of numerical runs have been performed and the strains are compared with the experimental results for circular blanks. A good agreement is obtained between the numerical and experimental results for the variable friction model used. 相似文献
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Ugur Gezer Ebru E. Y?rüker Metin Keskin Cemil Burak Kulle Yoganiranjana Dharuman Stefan Holdenrieder 《International journal of molecular sciences》2015,16(12):29654-29662
Circulating nucleic acids (CNAs) are under investigation as a liquid biopsy in cancer as potential non-invasive biomarkers, as stable structure in circulation nucleosomes could be valuable sources for detection of cancer-specific alterations in histone modifications. Our interest is in histone methylation marks with a focus on colorectal cancer, one of the leading cancers respective the incidence and mortality. Our previous work included the analysis of trimethylations of lysine 9 on histone 3 (H3K9me3) and of lysine 20 on histone 4 (H4K20me3) by chromatin immuno- precipitation-related PCR in circulating nucleosomes. Here we asked whether global immunologic measurement of histone marks in circulation could be a suitable approach to show their potential as biomarkers. In addition to H3K9me3 and H4K20me3 we also measured H3K27me3 in plasma samples from CRC patients (n = 63) and cancer free individuals (n = 40) by ELISA-based methylation assays. Our results show that of three marks, the amounts of H3K27me3 (p = 0.04) and H4K20me3 (p < 0.001) were significantly lower in CRC patients than in healthy controls. For H3K9me3 similar amounts were measured in both groups. Areas under the curve (AUC) in receiver operating characteristic (ROC) curves indicating the power of CRC detection were 0.620 for H3K27me3, 0.715 for H4K20me3 and 0.769 for the combination of both markers. In conclusion, findings of this preliminary study reveal the potential of blood-based detection of CRC by quantification of histone methylation marks and the additive effect of the marker combination. 相似文献
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In this paper, we investigate the influence of hypercapnia on the early and late phases of the phrenic neurogram using the matching pursuit (MP) method in the decebrated piglets. The phrenic neurogram was recorded from 8 piglets (4-7 days old) during control (40% O2 with 5% end-tidal CO2), the mild hypercapnia (40% O2 with 7% end-tidal CO2), and the severe hypercapnia (40% O2 with 15% end-tidal CO2). The time-frequency representations, atoms, of the phrenic neurogram are calculated from the 5 consecutive phrenic neurogram burst for each piglet for each condition using the MP method after vagotomy and chemodenervation. Our results show that the energy percentage of atoms representing the nonperiodic neural activities (NPNAs) significantly increased when the CO2 concentration was shifted from 7% to 15% in the early phase (the first half) of the phrenic neurogram. In addition, the energy percentage of atoms representing the periodic neural activities (PNAs) decreased in the late phase (the second half) when the CO2 concentration was shifted from 7% to 15% (p < 0.01). As a summary, our result suggest that hypercapnia results in significant changes in the phrenic neurogram, an output of the respiratory neural networks in the medulla, both in time and frequency domians during early maturation. 相似文献
116.
In this study, bacteriocins from two Lactococcus lactis subsp. lactis isolates from raw milk samples in Turkey designated OC1 and OC2, respectively, were characterized and identified. The activity spectra of the bacteriocins were determined by using different indicator bacteria including Listeria, Bacillus and Staphylococcus spp. Bacteriocins were tested for their sensitivity to different enzymes, heat treatments and pH values. Loss of bacteriocin activities after alpha-amylase treatment suggested that they form aggregates with carbohydrates. Molecular masses of the purified bacteriocins were determined by SDS-PAGE. PCR amplification was carried out with specific primers for the detection of their structural genes. As a result of these studies, the two bacteriocins were characterized as nisin and lacticin 481, respectively. Examination of plasmid contents of the isolates and the results of plasmid curing and conjugation experiments showed that in L. lactis subsp. lactis OC1 strain the 39.7-kb plasmid is responsible for nisin production, lactose fermentation and proteolytic activity, whereas the 16.0-kb plasmid is responsible for lacticin 481 production and lactose fermentation in L. lactis subsp. lactis OC2 strain. 相似文献
117.
Concentration of phenolic compounds from apple pomace extracts by nanofiltration at lab and pilot scale with a techno‐economic assessment 下载免费PDF全文
Maarten Uyttebroek Pieter Vandezande Miet Van Dael Sam Vloemans Bart Noten Bas Bongers Wim Porto‐Carrero Maria Muñiz Unamunzaga Metin Bulut Bert Lemmens 《Journal of food process engineering》2018,41(1)
Apple pomace can be used as resource for the extraction of phenolic compounds with antioxidant properties. Pressing of apple in juice and pomace at lab scale in open air (aerobic) and under N2 atmosphere (anaerobic) showed a recovery of phenolic compounds of 85% in juice and pomace after anaerobic pressing, compared to 43% after aerobic pressing, indicating loss of phenolic compounds by oxidation and the advantage of anaerobic over aerobic pressing. After a membrane screening and concentration test at lab scale, the commercial nanofiltration membrane NFX was selected to concentrate phenolic compounds in an ethanol : water extract of apple pomace. At pilot scale, the concencentration of 10 selected phenolic compounds and quinic acid increased from 59.5 mg/L in the ethanol : water extract to 1256.1 mg/L in the final retentate, that is, by a factor 21.1. The volume of the crude extract was reduced by a factor of 28.5 during the filtration, indicating some loss of phenolic compounds during pilot testing due to membrane fouling or oxidation of polyphenols. The pilot concentration test using a spiral‐wound membrane module showed good flux and concentration of phenolic compounds, indicating the technical feasibility of membrane technology for efficient concentration of polyphenols in an ethanol : water extraction solvent. Unfortunately, the extraction and concentration process was not economically feasible under the assumptions made.
Practical applications
The valorization of food waste for the production of high‐added value products is an increasingly hot topic. Phytochemicals are present in relatively low concentration in the fruit matrix, and concentration in the extraction solvent is necessary to develop an industrially relevant process. In this study, membrane filtration was selected for concentration due to its low energy consumption and mild processing conditions compared to other technologies. Membrane screening and testing at lab and pilot scale with techno‐economic assessment can be used by food and nutraceutical industries to evaluate membrane technology for concentration of phytochemicals extracted from agroindustrial by‐products. 相似文献118.
119.
D. Akçay Perdahcıoğlu M. Doreille A. de Boer T. Ludwig 《International journal for numerical methods in engineering》2013,96(6):390-404
A common mesh refinement‐based coupling technique is embedded into a component mode synthesis method, Craig–Bampton. More specifically, a common mesh is generated between the non‐conforming interfaces of the coupled structures, and the compatibility constraints are enforced on that mesh via L2‐minimization. This new integrated method is suitable for structural dynamic analysis problems where the substructures may have non‐conforming curvilinear and/or surface interface meshes. That is, coupled substructures may have different element types such as shell, solid, and/or beam elements. The proposed method is implemented into a commercial finite element software, B2000++, and its demonstration is carried out using an academic and industry oriented test problems. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
120.
Adsorption of Remazol Red B on activated carbon prepared from olive stone and commercial activated carbon from aqueous solutions was compared. Different activating agent (ZnCl2) amounts and adsorbent particle size were studied to optimize adsorbent surface area. The adsorptive property of commercial activated carbon and activated carbon prepared from olive stone were investigated in terms of adsorbent dose, temperature, equilibrium time and pH. Then the obtained results were compared for all parameters, According to the results, the equilibrium time, optimum pH, adsorbent dosage were found 60 min, pH < 3–4 and 1.0 g/50 ml respectively. Lower adsorption capacity for RRB on activated carbon prepared from olive stone was found. The kinetic data for both adsorbents supports pseudo-second order model (r2 > 0.99) and intra-particle model (r2 > 0.95) but the first order kinetic model did not adequately fit to the experimental values (r2 < 0.76). The equilibrium adsorption data were interpreted using Langmuir and Freundlich models. The adsorption of Remazol Red B was better represented by the Langmuir equation. In addition, the thermodynamic parameters, standard free energy (ΔG°), standard enthalpy (ΔH°), standard entropy (ΔS°) of the adsorption process were calculated for both adsorbents. To reveal the adsorptive characteristics of the produced active carbon, surface area measurements were carried out and structural analysis was performed using SEM-EDS. 相似文献