Wild edible fruit diversity and its significance in the livelihood of indigenous tribals: Evidence from eastern India

A number of wild plants, used by rural and tribal populations and contributing significantly to their livelihood and food security have escaped recognition and scientific inquiry. Their distribution, conservation, mode of harvest by locals and optimal use require region-specific assessment in order to integrate them into developmental interventions. This study analyzed the collection, consumption, sale and income from edible forest fruits in 49 tribal villages spread over five districts of Orissa State in eastern India. Density, dominance and diversity of species yielding wild fruit were measured by studying ecological parameters in the sample plots. We estimated an average of 48 fruit plants per hectare of deciduous forests. Fifty-six wild edible fruit species belonging to 40 genera in 26 families were recorded in the study region, many of which have multiple uses. Indigenous fruits formed part of the family diet with average annual consumption of 73 kg per household. Sale of wild fruits contributed 15 % of income for tribal households. Despite their good knowledge of indigenous fruits, the tribal populations have not adopted fruit tree farming which would enhance their nutrition and income.     

Antioxidant activities and nutritional properties of Jeonyak prepared with beef bone stock and gelatin

The antioxidant activities and nutritional properties of Jeonyak made with beef bone stock and gelatin were investigated. Traditional Jeonyak (J1) was prepared with beef bone stock, whereas modified Jeonyak was made with gelatin and honey (J2) or with gelatin and oligosaccharides (J3). All 3 kinds of Jeonyak had antioxidant activities and J1 had the highest activity. The α-glucosidase inhibitory activity was measured to investigate the antidiabetic effect and J3 had the highest inhibitory activity of 80.47%. Glycine was the most abundant amino acid in all 3 kinds of Jeonyak. Both traditional and modified Jeonyaks have antioxidative and antidiabetic effects, suggesting that Jeonyak can be used as an effective traditional functional food.     

Functional Properties of Plasticized Bio-Based Poly(Lactic Acid)_Poly(Hydroxybutyrate) (PLA_PHB) Films for Active Food Packaging

Fully bio-based and biodegradable active films based on poly(lactic acid) (PLA) blended with poly(3-hydroxybutyrate) (PHB) and incorporating lactic acid oligomers (OLA) as plasticizers and carvacrol as active agent were extruded and fully characterized in their functional properties for antimicrobial active packaging. PLA_PHB films showed good barrier to water vapor, while the resistance to oxygen diffusion decreased with the addition of OLA and carvacrol. Their overall migration in aqueous food simulant was determined and no significant changes were observed by the addition of carvacrol and OLA to the PLA_PHB formulations. However, the effect of both additives in fatty food simulant can be considered a positive feature for the potential protection of foodstuff with high fat content. Moreover, the antioxidant and antimicrobial activities of the proposed formulations increased by the presence of carvacrol, with enhanced activity against Staphylococcus aureus if compared to Escherichia coli at short and long incubation times. These results underlined the specific antimicrobial properties of these bio-films suggesting their applicability in active food packaging.     

Application of full-factorial design for optimization of the moisture determination in wheat flour using infrared scales

We studied the parameters that may affect the analysis of the moisture content in wheat flour using infrared scales. The parameters were studied by one-factor at a time methodology and then, by full-factorial design (2³). The best condition using infrared scales occurred at 120 °C, with a sample weight of 1.5 g and an exposure time to infrared radiation of 6 min. We observed the interaction effect between the three factors studied. The analysis time was 60 times lower than classical method. The optimized method for determination of the moisture content in wheat flour using infrared scales was validated to range between 9 and 17 %.     

Comparison of Different Mechanical Methods for the Modification of the Egg White Protein Ovomucin,Part B: Molecular Aspects

It is indispensable to modify the physical properties of egg white prior to a fractionation of the included bio-functional proteins. It was already demonstrated that this can be realized with mechanical devices. However, until now, it was not clear by which kind of molecular changes this is accompanied. Thus, this study reports on the molecular changes in egg white proteins induced by various mechanical treatments (high-pressure homogenizer, colloid mill, toothed disc dispersing machine). Evaluation criteria were the particle size of the long-chain protein ovomucin, the content of thiol groups, and disulfide bridges in egg white as well as the amount of free lysozyme. In general, it was shown that these treatments led to changes in the molecular structure and that the obtained modifications were more pronounced the higher the applied energy was. In detail, it was found that the applied mechanical forces in the experimental range of this study were able to disrupt strong covalent bonds in the fibrillar protein ovomucin. Additionally, the bio-functional protein lysozyme that is partly entrapped in the natural egg white structure was released by the applied forces. Summing up, this study generates comprehensive knowledge concerning the underlying mechanisms that enable the release of lysozyme as well as the use of egg white for fractionation processes.     

A First Approach of Using Ultrasound as an Alternative for Blanching in Vacuum-Packaged Potato Strips

The effect of ultrasound (US) (40 kHz, 200 W, 3 min), blanching (85 °C, 3.5 min), and the combination of both methods was evaluated on the quality of vacuum-packaged potato strips stored at 3 ± 1 °C for up to 10 days. For this study, two cultivars of potatoes were assessed. For blanched Agata samples, the lightness (L*) decreased over 12 % (p < 0.05). Moreover, their hue increased up to 100, obtaining lesser yellow potato strips. In contrast, US did not affect the hue values. The losses of firmness of blanched potato strips were notable (35 % for Agata and 51 % for Agria), whereas US did not change this property (p < 0.05). Nevertheless, no significant differences were found in the total starch content at 10 days. Agata and Agria showed different metabolic behaviors of sucrose in the refrigerated storage. Therefore, Agria cultivar retained better color after frying. These results suggest that US had less impact on color and improve the firmness in vacuum-packaged potato strips with no added chemicals.     

Cyanidin-3-O-(2″-xylosyl)-glucoside, an anthocyanin from Siberian ginseng (Acanthopanax senticosus) fruits, inhibits UVB-induced COX-2 expression and AP-1 transactivation

Cyanidin-3-O-(2″-xylosyl)-glucoside (C-3-O-(2″-xylosyl)-G) was analyzed as an active constituent from the fruit of Siberian ginseng (Acanthopanax senticosus) using a HPLC diode array detection-electrospray ionization/mass spectrometry analysis system and the effect of C-3-O-(2″-xylosyl)-G on UVB-induced inflammatory signaling in JB6 P+ cells was investigated. C-3-O-(2″-xylosyl)-G inhibited UVB-induced cyclooxygenase (COX)-2 expression and promoter binding activity in JB6 P+ cells and JB6 P+ cells stably transfected with the COX-2 luciferase reporter plasmid. It inhibited both the UVB-induced activator protein-1 (AP-1) and nuclear factor (NF)-κB transactivation in JB6 P+ cells stably transfected with the AP-1 and NF-κB luciferase reporter plasmids. Additionally, C-3-O-(2″-xylosyl)-G significantly suppressed UVB-induced upregulated phosphorylation of c-Jun N terminal kinase, MEK/extracellular signaling kinase, and mitogen activated protein kinase kinase (MAPKK) 3/6 in JB6 P+ cells. These results indicate that C-3-O-(2″-xylosyl)-G may be a promising chemopreventive material that acts by suppressing COX-2 expression and AP-1 and NF-κB transactivation and JNK, MAPKK3/6, and MEK/ERK1/2 phosphorylation.     

The impact of NERICA adoption on productivity and poverty of the small-scale rice farmers in the Gambia

The study assessed the causal effects of NERICA adoption on rice yields and income, using country-wide data of 600 rice farmers in The Gambia. To control for differences in socio-demographic and environmental characteristics of adopters and non-adopters and enable a causal interpretation of NERICA adoption on the variables of interest, the study used the counterfactual outcome framework to control for such differences. The results, based on observed sample estimates, showed significant differences in rice yields and income between the NERICA adopters and non-adopters. Further, the results of the framework, based on the Local Average Treatment Effect (LATE) estimates, which allowed a causal interpretation, showed that NERICA adoption significantly increased average rice yields and annual income of small-scale rice farmers by 157 kg per hectare and $148, respectively.     

Quantitative Determination for the Major Volatile Organic Compounds of <Emphasis Type="Italic">Tuber melanosporum</Emphasis> Fermentation System by Distillation–Solid-Phase Extraction–Gas Chromatography

A method using distillation–solid-phase extraction coupled with gas chromatography and flame ionization detector was developed for assaying the major volatile organic compounds (VOCs) (i.e., 3-methyl-1-butanol, 2-methyl-1-butanol, and 2-phenylethanol) in the Tuber melanosporum fermentation system. Satisfied linearity, precision, accuracy, and recovery obtained in the method validation test indicate that the method was simple, accurate, and reproducible. Furthermore, the developed analytical method was used for the dynamic monitoring of the T. melanosporum fermentation process, and the highest amount of major VOCs was occurred on days 9 and 10. This method can be employed to monitor the VOCs in the fermentation process and thus to evaluate the VOC quality of the truffle fermentation system.     

Optimization and Validation of a UV–HPLC Method for Vitamin C Determination in Strawberries (<Emphasis Type="Italic">Fragaria ananassa</Emphasis> Duch.), Using Experimental Designs

Vitamin C or total ascorbic acid (TAA) in fruits can be assumed as ascorbic acid (AA) plus dehydroascorbic acid (DHA) content. The aim of this work was to optimize and validate, using experimental designs, a new high-performance liquid chromatographic method for vitamin C determination in strawberries. The mobile phase (MP) consisted of a 0.03 M sodium acetate/acetic acid buffer, 5% methanol. For optimization, a Box–Behnken design was used (three factors at three levels: (a) pH of MP, 3.8–5.8; (b) wavelength, 240–270 nm; and (c) flow rate, 0.5–1.2 ml min⁻¹). Responses were: AA and TAA areas, peak widths, and retention times. A global optimization was performed using the Derringer desirability function, and a value of 0.84 was reached for the combination of design factors: A = 5.8, B = 251 nm, and C = 1.15 ml min⁻¹. Method validation, using AA standard solutions, included: linearity study, limits of detection and quantification, and calibration and analytical sensitivity quantifications. Precision and accuracy were studied in strawberry extracts. The coefficients of variation (percent) were: AA, 1.5%; TAA, 1.8%, and DHA, 4.9%. Accuracy was evaluated with AA standard spiked in 30–150% range of the expected amount of analyte in real samples. The joint confidence elliptical region test and t test were employed for the study of the difference between recoveries (percent) and the ideal 100%. The robustness was analyzed using a fractional factorial design (3⁴⁻²), and an AA recovery study after slight changes in operative variables was performed. The results indicate that the optimized method was linear, sensible, precise, accurate, and robust.