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61.
62.
Michaela Oeller Sandra Laner-Plamberger Linda Krisch Eva Rohde Dirk Strunk Katharina Schallmoser 《International journal of molecular sciences》2021,22(10)
Numerous cell-based therapeutics are currently being tested in clinical trials. Human platelet lysate (HPL) is a valuable alternative to fetal bovine serum as a cell culture medium supplement for a variety of different cell types. HPL as a raw material permits animal serum-free cell propagation with highly efficient stimulation of cell proliferation, enabling humanized manufacturing of cell therapeutics within a reasonable timeframe. Providers of HPL have to consider dedicated quality issues regarding identity, purity, potency, traceability and safety. Release criteria have to be defined, characterizing the suitability of HPL batches for the support of a specific cell culture. Fresh or expired platelet concentrates from healthy blood donors are the starting material for HPL preparation, according to regulatory requirements. Pooling of individual platelet lysate units into one HPL batch can balance donor variation with regard to essential platelet-derived growth factors and cytokines. The increasingly applied pathogen reduction technologies will further increase HPL safety. In this review article, aspects and regulatory requirements of whole blood donation and details of human platelet lysate manufacturing are presented. International guidelines for raw materials are discussed, and defined quality controls, as well as release criteria for safe and GMP-compliant HPL production, are summarized. 相似文献
63.
R. Edenharder Claudia Speth Michaela Decker Karl Ludwig Platt 《European Food Research and Technology》1998,207(6):464-471
Nine naphthoquinones, 19 anthraquinones, and nine structurally related monoketonic compounds such as anthrone, xanthone,
etc., inhibited mutagenicity induced by 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in Salmonella typhimurium TA 98 in the presence of rat liver S9 with distinct structure-activity relationships. A carbonyl function was a prerequisite
for antimutagenicity while, in general, anthraquinones (IC50 values: 2.3–>213 nmol/ml top agar) were more potent antimutagens than structurally related monoketonic compounds (IC50 values: 25.3–94.9 nmol/ml top agar) and naphthoquinones (IC50 values: 3.7–90.7 nmol/ml top agar). The parent compounds and methyl substituted derivatives were already the most potent while
introduction of polar substituents such as COOH and SO3H considerably reduced antimutagenicity. Introduction of OH functions had equivocal effects: with increasing numbers, antimutagenic
potencies were concomitantly reduced; however, anthraflavic acid, chrysazin, quinizarin, and especially 5,8-dihydroxy-1,4-naphthoquinone
were more potent than the parent compounds. The patterns of inhibition by quinones of 7-ethoxyresorufin-O-dealkylase activities in rat liver microsomes, linked to cytochrome P-450-dependent oxidation of IQ to N-hydroxy-IQ (N-OH-IQ), were in general identical with those obtained in the Salmonella/reversion assay except for chrysophanic acid, emodin, and some naphthoquinones which were very potent in this assay (IC50: 0.20–45.0 μM). On the other hand, mutagenicity of N-OH-IQ in S. typhimurium TA 98NR was not inhibited by nonpolar quinones (except 1,4-naphthoquinone) but rather by polar compounds and especially by
hydroxyquinones (IC50 values: 5.3–106.7 nmol/ml top agar or not reached). Inhibition of mutagenic activities of IQ, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, 2-amino-6-methyldipyrido[1,2-a:3′,2′-d]imidazole, and 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chrysazin, chrysophanic acid, physicon, and purpurin varied, but no clearcut structure-activity relationships of
the mutagens were observed.
Received: 29 May 1998 相似文献
64.
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66.
Florian M. Dato Dr. Miriam Sheikh Rocky Z. Uhl Alexandra W. Schüller Dr. Michaela Steinkrüger Dr. Peter Koch Dr. Jörg‐Martin Neudörfl Prof. Michael Gütschow Prof. Bernd Goldfuss Dr. Markus Pietsch 《ChemMedChem》2018,13(17):1833-1847
Cholesterol esterase (CEase), a serine hydrolase thought to be involved in atherogenesis and thus coronary heart disease, is considered as a target for inhibitor development. We investigated recombinant human and murine CEases with a new fluorometric assay in a structure–activity relationship study of a small library of ω‐phthalimidoalkyl aryl ureas. The urea motif with an attached 3,5‐bis(trifluoromethyl)phenyl group and the aromatic character of the ω‐phthalimide residue were most important for inhibitory activity. In addition, an alkyl chain composed of three or four methylene groups, connecting the urea and phthalimide moieties, was found to be an optimal spacer for inhibitors. The so‐optimized compounds 2 [1‐(3,5‐bis(trifluoromethyl)phenyl)‐3‐(3‐(1,3‐dioxoisoindolin‐2‐yl)propyl)urea] and 21 [1‐(3,5‐bis(trifluoromethyl)phenyl)‐3‐(4‐(1,3‐dioxoisoindolin‐2‐yl)butyl)urea] exhibited dissociation constants (Ki) of 1–19 μm on the two CEases and showed either a competitive ( 2 on the human enzyme and 21 on the murine enzyme) or a noncompetitive mode of inhibition. Two related serine hydrolases—monoacylglycerol lipase and fatty acid amide hydrolase—were inhibited by ω‐phthalimidoalkyl aryl ureas to a lesser extent. 相似文献
67.
Tobias?MettlerEmail authorView authors OrcID profile Michaela?Sprenger Robert?Winter 《欧洲信息系统杂志》2017,26(5):451-468
Changing demands in society and the limited capabilities of health systems have paved the way for robots to move out of industrial contexts and enter more human-centered environments such as health care. We explore the shared beliefs and concerns of health workers on the introduction of autonomously operating service robots in hospitals or professional care facilities. By means of Q-methodology, a mixed research approach specifically designed for studying subjective thought patterns, we identify five potential end-user niches, each of which perceives different affordances and outcomes from using service robots in their working environment. Our findings allow for better understanding resistance and susceptibility of different users in a hospital and encourage managerial awareness of varying demands, needs, and surrounding conditions that a service robot must contend with. We also discuss general insights into presenting the Q-methodology results and how an affordance-based view could inform the adoption, appropriation, and adaptation of emerging technologies. 相似文献
68.
Cyril Brom Vít Šisler Michaela Slussareff Tereza Selmbacherová Zdeněk Hlávka 《International Journal of Computer-Supported Collaborative Learning》2016,11(3):313-348
Despite the alleged ability of digital game-based learning (DGBL) to foster positive affect and in turn improve learning, the link between affectivity and learning has not been sufficiently investigated in this field. Regarding learning from team-based games with competitive elements, even less is known about the relationship between competitiveness (as a dispositional trait) and induced positive affect. In this media comparison study with between-subject design, participants (N?=?325; high school and college students) learned about the EU’s policy agenda by means of a debate-based method delivered through one of three educational media: a) through a social role-playing game with competitive elements played on computers, b) through a very similar game played without computers and c) through a non-game workshop. Unlike many previous DGBL studies, this study used participant randomization and strived to address the teacher effect and the length of exposure effect, while also using the same learning materials and a very similar educational method for all three treatments. Both games induced comparatively higher generalized positive affect and flow. Participants also learned more with the games. Positive affect, but not flow, mediated the influence of educational media on learning gains. Participants’ competitiveness was partly related to positive affect and experiencing flow but unrelated to learning gains. These outcomes held both when the game was played using computers, as well as without them. The study indicates that the ability of an educational intervention to instigate positive affect is an important feature that should be considered by educational designers. 相似文献
69.
Michaela N. Ess 《Aerosol science and technology》2019,53(1):29-44
Flame-generated soot from miniCAST burners is increasingly being used in academia and industry as engine exhaust soot surrogate for atmospheric studies and instrument calibration. Previous studies have found that elemental carbon (EC) content of miniCAST soot is proportional to the mean particle size. Here, the characterization of a prototype miniCAST generator (5201 Type BC), which was designed to decouple the soot composition from the particle size and produce soot particles with high EC and BC content in a large size range, is reported. This prototype may operate either in a diffusion-flame or a partially premixed-flame mode, an option that was not available in former models. It was confirmed that soot properties, such as EC content and Ångström absorption exponent (AAE), were linked to the overall flame composition. In particular, combustion under fuel-rich conditions provided particles with size coupled to the EC fraction and AAE, i.e. smaller particles exhibited a lower EC fraction and higher AAE. In contrast, with fuel-lean diffusion flames and especially with premixed flames under near overall stoichiometric conditions small particles (down to 30?nm) with high EC/TC ratios (>60%) and low AAE (≈1.4) could be generated even without any thermal after-treatment. This new source might thus serve in the future as a useful surrogate for engine exhaust emissions and help to improve calibration procedures of common aerosol instruments.
Copyright © 2018 The Author(s). Published with license by Taylor & Francis Group, LLC 相似文献
70.
Matthew R Hartings Noah Benjamin Floriene Briere Maria Briscione Omar Choudary Tamra L Fisher Laura Flynn Elizabeth Ghias Michaela Harper Nader Khamis Catherine Koenigsknecht Klare Lazor Steven Moss Elaine Robbins Susan Schultz Samiye Yaman Luke M Haverhals Paul C Trulove Hugh C De Long Abigail E Miller Douglas M Fox 《Science and Technology of Advanced Materials》2013,14(6)
AbstractA technique was developed for preparing a novel material that consists of gold nanoparticles trapped within a fiber of unfolded proteins. These fibers are made in an aqueous solution that contains HAuCl4 and the protein, bovine serum albumin (BSA). By changing the ratio of gold to BSA in solution, two different types of outcomes are observed. At lower gold to BSA ratios (30–120), a purple solution results after heating the mixture at 80 °C for 4 h. At higher gold to BSA ratios (130–170), a clear solution containing purple fibers results after heating the mixture at 80 °C for 4 h. UV–Vis spectroscopy and light scattering techniques show growth in nanocolloid size as gold to BSA ratio rises above 100. Data indicate that, for the higher gold to BSA ratios, the gold is sequestered within the solid material. The material mass, visible by eye, appears to be an aggregation of smaller individual fibers. Scanning electron microscopy and transmission electron microscopy indicate that these fibers are primarily one-dimensional aggregates, which can display some branching, and can be as narrow as 400 nm in size. The likely mechanism for the synthesis of the novel material is discussed. 相似文献