全文获取类型
收费全文 | 516篇 |
免费 | 30篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 161篇 |
金属工艺 | 2篇 |
机械仪表 | 17篇 |
建筑科学 | 23篇 |
矿业工程 | 1篇 |
能源动力 | 15篇 |
轻工业 | 61篇 |
水利工程 | 3篇 |
无线电 | 12篇 |
一般工业技术 | 55篇 |
冶金工业 | 89篇 |
自动化技术 | 106篇 |
出版年
2023年 | 7篇 |
2022年 | 30篇 |
2021年 | 42篇 |
2020年 | 16篇 |
2019年 | 9篇 |
2018年 | 15篇 |
2017年 | 19篇 |
2016年 | 22篇 |
2015年 | 23篇 |
2014年 | 28篇 |
2013年 | 25篇 |
2012年 | 22篇 |
2011年 | 29篇 |
2010年 | 27篇 |
2009年 | 37篇 |
2008年 | 16篇 |
2007年 | 18篇 |
2006年 | 16篇 |
2005年 | 17篇 |
2004年 | 14篇 |
2003年 | 11篇 |
2002年 | 12篇 |
2001年 | 7篇 |
2000年 | 12篇 |
1999年 | 9篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 1篇 |
排序方式: 共有546条查询结果,搜索用时 677 毫秒
21.
Claire S. Whyte Akriti Rastogi Ellis Ferguson Michela Donnarumma Nicola J. Mutch 《International journal of molecular sciences》2022,23(6)
Loss of fibrinogen is a feature of trauma-induced coagulopathy (TIC), and restoring this clotting factor is protective against hemorrhages. We compared the efficacy of cryoprecipitate, and of the fibrinogen concentrates RiaSTAP® and FibCLOT® in restoring the clot integrity in models of TIC. Cryoprecipitate and FibCLOT® produced clots with higher maximal absorbance and enhanced resistance to lysis relative to RiaSTAP®. The fibrin structure of clots, comprising cryoprecipitate and FibCLOT®, mirrored those of normal plasma, whereas those with RiaSTAP® showed stunted fibers and reduced porosity. The hemodilution of whole blood reduced the maximum clot firmness (MCF) as assessed by thromboelastography. MCF could be restored with the inclusion of 1 mg/mL of fibrinogen, but only FibCLOT® was effective at stabilizing against lysis. The overall clot strength, measured using the Quantra® hemostasis analyzer, was restored with both fibrinogen concentrates but not cryoprecipitate. α2antiplasmin and plasminogen activator inhibitor-1 (PAI-1) were constituents of cryoprecipitate but were negligible in RiaSTAP® and FibCLOT®. Interestingly, cryoprecipitate and FibCLOT® contained significantly higher factor XIII (FXIII) levels, approximately three-fold higher than RiaSTAP®. Our data show that 1 mg/mL fibrinogen, a clinically achievable concentration, can restore adequate clot integrity. However, FibCLOT®, which contained more FXIII, was superior in normalizing the clot structure and in stabilizing hemodiluted clots against mechanical and fibrinolytic degradation. 相似文献
22.
23.
Federico Bolognesi Nicola Fazio Filippo Boriani Viscardo Paolo Fabbri Davide Gravina Francesca Alice Pedrini Nicoletta Zini Michelina Greco Michela Paolucci Maria Carla Re Sofia Asioli Maria Pia Foschini Antonietta DErrico Nicola Baldini Claudio Marchetti 《International journal of molecular sciences》2022,23(3)
Defects of the peripheral nervous system are extremely frequent in trauma and surgeries and have high socioeconomic costs. If the direct suture of a lesion is not possible, i.e., nerve gap > 2 cm, it is necessary to use grafts. While the gold standard is the autograft, it has disadvantages related to its harvesting, with an inevitable functional deficit and further morbidity. An alternative to autografting is represented by the acellular nerve allograft (ANA), which avoids disadvantages of autograft harvesting and fresh allograft rejection. In this research, the authors intend to transfer to human nerves a novel technique, previously implemented in animal models, to decellularize nerves. The new method is based on soaking the nerve tissues in decellularizing solutions while associating ultrasounds and freeze–thaw cycles. It is performed without interrupting the sterility chain, so that the new graft may not require post-production γ-ray irradiation, which is suspected to affect the structural and functional quality of tissues. The new method is rapid, safe, and inexpensive if compared with available commercial ANAs. Histology and immunohistochemistry have been adopted to evaluate the new decellularized nerves. The study shows that the new method can be applied to human nerve samples, obtaining similar, and, sometimes better, results compared with the chosen control method, the Hudson technique. 相似文献
24.
Rossella Di Guida Angela Casillo Juan M. Toms Susana Merino Maria Michela Corsaro 《International journal of molecular sciences》2022,23(3)
Aeromonas species are found in the aquatic environment, drinking water, bottled mineral water, and different types of foods, such as meat, fish, seafood, or vegetables. Some of these species are primary or opportunistic pathogens for invertebrates and vertebrates, including humans. Among the pathogenic factors associated with these species, there are the lipopolysaccharides (LPSs). LPSs are the major components of the external leaflet of Gram-negative bacterial outer membrane. LPS is a glycoconjugate, generally composed of three portions: lipid A, core oligosaccharide, and O-specific polysaccharide or O-antigen. The latter, which may be present (smooth LPS) or not (rough LPS), is the most exposed part of the LPS and is involved in the pathogenicity by protecting infecting bacteria from serum complement killing and phagocytosis. The O-antigen is a polymer of repeating oligosaccharide units with high structural variability, particularly the terminal sugar, that confers the immunological specificity to the O-antigen. In this study, we established the structure of the O-chain repeating unit of the LPS from Aeromonas bivalvium strain 868 ET (=CECT 7113T = LMG 23376T), a mesophilic bacterium isolated from cockles (Cardium sp.) and obtained from a retail market in Barcelona (Spain), whose biosynthesis core LPS cluster does not contain the waaE gene as most of Aeromonas species. After mild acid hydrolysis, the lipid A was removed by centrifugation and the obtained polysaccharide was fully characterized by chemical analysis and NMR spectroscopy. The polymer consists of a heptasaccharide repeating unit containing D-GalNAc, L-Rha, D-GlcNAc, and D-FucNAc residues. 相似文献
25.
Filippo Piccinini Michela Pierini Enrico Lucarelli Alessandro Bevilacqua 《Journal of materials science. Materials in medicine》2014,25(10):2395-2410
The analysis of cell confluence and proliferation is essential to design biomaterials and scaffolds to use as bone substitutes in clinical applications. Accordingly, several approaches have been proposed in the literature to estimate the area of the scaffold covered by cells. Nevertheless, most of the approaches rely on sophisticated equipment not employed for routine analyses, while the rest of them usually do not provide significant statistics about the cell distribution. This research aims at studying confluence and proliferation of mesenchymal stromal cells (MSC) adherent on OSPROLIFE®, a commercial biomaterial in the form of granules. In particular, we propose a Computer Vision approach that can routinely be employed to monitor the surface of the single granules covered by cells because only a standard widefield fluorescent microscope is required. In order to acquire significant statistics data, we analyse wide-area images built by using MicroMos v2.0, an updated version of a previously published software specific for stitching brightfield and phase-contrast images manually acquired via a widefield microscope. In particular, MicroMos v2.0 permits to build accurate “mosaics” of fluorescent images, after correcting vignetting and photo-bleaching effects, providing a consistent representation of a sample region containing numerous granules. Then, our method allows to make automatically a statistically significant estimate of the percentage of the area of the single granules covered by cells. Finally, by analysing hundreds of granules at different time intervals we also obtained reliable data regarding cell proliferation, confirming that not only MSC adhere onto the OSPROLIFE® granules, but even proliferate over time. 相似文献
26.
Nowadays two main approaches are being pursued to reduce energy consumption of networks: the use of sleep modes in which devices enter a low-power state during inactivity periods, and the adoption of energy proportional mechanisms where the device architecture is designed to make energy consumption proportional to the actual load. Common to all the proposals is the evaluation of energy saving performance by means of simulation or experimental evidence, which typically consider a limited set of benchmarking scenarios. 相似文献
27.
Sara Salucci Sabrina Burattini Michela Battistelli Valentina Baldassarri Maria Cristina Maltarello Elisabetta Falcieri 《International journal of molecular sciences》2013,14(1):532-546
Ultraviolet B (UVB) radiation acts as a strong apoptotic trigger in many cell types, in tumor and normal cells. Several studies have demonstrated that UVB-induced cell death occurs through the generation of reactive oxygen species. The consequent oxidative stress includes the impairment of cellular antioxidants, the induction of DNA damage and the occurrence of apoptosis. In this review, we investigated UVB apoptotic action in various cell models by using ultrastructural, molecular and cytofluorimetric techniques. Myeloid leukemia HL-60, T-lymphoblastoid Molt-4 and myelomonocytic U937 human cells, generally affected by apoptotic stimuli, were studied. Human chondrocytes and C2C12 skeletal muscle cells, known to be more resistant to damage, were also considered. All of them, when exposed to UVB radiation, revealed a number of characteristic apoptotic markers. Membrane blebbing, cytoplasm shrinkage and chromatin condensation were detected by means of electron microscopy. DNA cleavage, investigated by using agarose gel electrophoresis and TUNEL reaction, was observed in suspended cells. Differently, in chondrocytes and in skeletal muscle cells, oligonucleosomic DNA fragmentation did not appear, even if a certain TUNEL positivity was detected. These findings demonstrate that UVB radiation appears to be an ideal tool to study the apoptotic behavior. 相似文献
28.
29.
30.
In a team negotiation context, the authors empirically explored how judgments of team-level trust are derived from individual-level trust. Basing their argument on both the negativity bias and the discontinuity effect, the authors posit that people will focus most on the least trustworthy individual member of a team when making judgments about collective team-level trust. Findings from two studies demonstrate that perceptions of team trust are indeed lower than the average ratings of individual trust and are statistically equivalent to the least trusted member. In addition, compared with average individual trust levels, perceptions of collective team trust were found to be more predictive of (a) impasse rates in distributive negotiations and (b) the level of joint gain in integrative negotiations. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献