全文获取类型
收费全文 | 8674篇 |
免费 | 477篇 |
国内免费 | 6篇 |
专业分类
电工技术 | 63篇 |
综合类 | 6篇 |
化学工业 | 1877篇 |
金属工艺 | 99篇 |
机械仪表 | 142篇 |
建筑科学 | 300篇 |
矿业工程 | 13篇 |
能源动力 | 231篇 |
轻工业 | 1647篇 |
水利工程 | 120篇 |
石油天然气 | 43篇 |
无线电 | 489篇 |
一般工业技术 | 1187篇 |
冶金工业 | 1413篇 |
原子能技术 | 23篇 |
自动化技术 | 1504篇 |
出版年
2024年 | 17篇 |
2023年 | 91篇 |
2022年 | 178篇 |
2021年 | 273篇 |
2020年 | 222篇 |
2019年 | 231篇 |
2018年 | 329篇 |
2017年 | 291篇 |
2016年 | 322篇 |
2015年 | 252篇 |
2014年 | 347篇 |
2013年 | 693篇 |
2012年 | 567篇 |
2011年 | 648篇 |
2010年 | 436篇 |
2009年 | 396篇 |
2008年 | 427篇 |
2007年 | 398篇 |
2006年 | 294篇 |
2005年 | 208篇 |
2004年 | 209篇 |
2003年 | 180篇 |
2002年 | 159篇 |
2001年 | 123篇 |
2000年 | 91篇 |
1999年 | 112篇 |
1998年 | 553篇 |
1997年 | 309篇 |
1996年 | 214篇 |
1995年 | 123篇 |
1994年 | 95篇 |
1993年 | 90篇 |
1992年 | 28篇 |
1991年 | 26篇 |
1990年 | 22篇 |
1989年 | 18篇 |
1988年 | 17篇 |
1987年 | 26篇 |
1986年 | 16篇 |
1985年 | 23篇 |
1984年 | 10篇 |
1983年 | 9篇 |
1982年 | 8篇 |
1981年 | 12篇 |
1980年 | 12篇 |
1978年 | 7篇 |
1977年 | 9篇 |
1976年 | 23篇 |
1973年 | 2篇 |
1967年 | 3篇 |
排序方式: 共有9157条查询结果,搜索用时 15 毫秒
51.
J. M. Martínez 《Computing》1987,39(4):307-325
We introduce a new method for solving Nonlinear Least Squares problems when the Jacobian matrix of the system is large and sparse. The main features of the new method are the following:
- The Gauss-Newton equation is “partially” solved at each iteration using a preconditioned Conjugate Gradient algorithm.
- The new point is obtained using a two-dimensional trust region scheme, similar to the one introduced by Bulteau and Vial.
52.
Luis O. Soto-Rojas B. Berenice Campa-Crdoba Charles R. Harrington Andrs Salas-Casas Mario Hernandes-Alejandro Ignacio Villanueva-Fierro Marely Bravo-Muoz Linda Garcs-Ramírez Fidel De La Cruz-Lpez Miguel ngel Ontiveros-Torres Goar Gevorkian Mar Pacheco-Herrero Jos Luna-Muoz 《International journal of molecular sciences》2021,22(7)
Alzheimer’s disease (AD) is a neurodegenerative disease, characterized histopathologically by intra-neuronal tau-related lesions and by the accumulation of amyloid β-peptide (Aβ) in the brain parenchyma and around cerebral blood vessels. According to the vascular hypothesis of AD, an alteration in the neurovascular unit (NVU) could lead to Aβ vascular accumulation and promote neuronal dysfunction, accelerating neurodegeneration and dementia. To date, the effects of insoluble vascular Aβ deposits on the NVU and the blood–brain barrier (BBB) are unknown. In this study, we analyze different Aβ species and their association with the cells that make up the NVU. We evaluated post-mortem AD brain tissue. Multiple immunofluorescence assays were performed against different species of Aβ and the main elements that constitute the NVU. Our results showed that there are insoluble vascular deposits of both full-length and truncated Aβ species. Besides, insoluble aggregates are associated with a decrease in the phenotype of the cellular components that constitute the NVU and with BBB disruption. This approach could help identify new therapeutic targets against key molecules and receptors in the NVU that can prevent the accumulation of vascular fibrillar Aβ in AD. 相似文献
53.
Olga Azevedo Filipa Cordeiro Miguel Fernandes Gago Gabriel Miltenberger-Miltenyi Catarina Ferreira Nuno Sousa Damio Cunha 《International journal of molecular sciences》2021,22(9)
Fabry disease (FD) is an X-linked lysosomal storage disorder caused by mutations of the GLA gene that result in a deficiency of the enzymatic activity of α-galactosidase A and consequent accumulation of glycosphingolipids in body fluids and lysosomes of the cells throughout the body. GB3 accumulation occurs in virtually all cardiac cells (cardiomyocytes, conduction system cells, fibroblasts, and endothelial and smooth muscle vascular cells), ultimately leading to ventricular hypertrophy and fibrosis, heart failure, valve disease, angina, dysrhythmias, cardiac conduction abnormalities, and sudden death. Despite available therapies and supportive treatment, cardiac involvement carries a major prognostic impact, representing the main cause of death in FD. In the last years, knowledge has substantially evolved on the pathophysiological mechanisms leading to cardiac damage, the natural history of cardiac manifestations, the late-onset phenotypes with predominant cardiac involvement, the early markers of cardiac damage, the role of multimodality cardiac imaging on the diagnosis, management and follow-up of Fabry patients, and the cardiac efficacy of available therapies. Herein, we provide a comprehensive and integrated review on the cardiac involvement of FD, at the pathophysiological, anatomopathological, laboratory, imaging, and clinical levels, as well as on the diagnosis and management of cardiac manifestations, their supportive treatment, and the cardiac efficacy of specific therapies, such as enzyme replacement therapy and migalastat. 相似文献
54.
Pablo Bonilla Joaquim Hernandez Esther Giraldo Miguel A. Gonzlez-Prez Ana Alastrue-Agudo Hoda Elkhenany María J. Vicent Xavier Navarro Michael Edel Victoria Moreno-Manzano 《International journal of molecular sciences》2021,22(11)
We currently lack effective treatments for the devastating loss of neural function associated with spinal cord injury (SCI). In this study, we evaluated a combination therapy comprising human neural stem cells derived from induced pluripotent stem cells (iPSC-NSC), human mesenchymal stem cells (MSC), and a pH-responsive polyacetal–curcumin nanoconjugate (PA-C) that allows the sustained release of curcumin. In vitro analysis demonstrated that PA-C treatment protected iPSC-NSC from oxidative damage in vitro, while MSC co-culture prevented lipopolysaccharide-induced activation of nuclear factor-κB (NF-κB) in iPSC-NSC. Then, we evaluated the combination of PA-C delivery into the intrathecal space in a rat model of contusive SCI with stem cell transplantation. While we failed to observe significant improvements in locomotor function (BBB scale) in treated animals, histological analysis revealed that PA-C-treated or PA-C and iPSC-NSC + MSC-treated animals displayed significantly smaller scars, while PA-C and iPSC-NSC + MSC treatment induced the preservation of β-III Tubulin-positive axons. iPSC-NSC + MSC transplantation fostered the preservation of motoneurons and myelinated tracts, while PA-C treatment polarized microglia into an anti-inflammatory phenotype. Overall, the combination of stem cell transplantation and PA-C treatment confers higher neuroprotective effects compared to individual treatments. 相似文献
55.
Aintzane Rueda-Martínez Aiara Garitazelaia Ariadna Cilleros-Portet Sergi Marí Rebeca Arauzo Jokin de Miguel Brbara P. Gonzlez-García Nora Fernandez-Jimenez Jose Ramon Bilbao Iraia García-Santisteban 《International journal of molecular sciences》2021,22(11)
Endometriosis is a common gynecological disorder that has been associated with endometrial, breast and epithelial ovarian cancers in epidemiological studies. Since complex diseases are a result of multiple environmental and genetic factors, we hypothesized that the biological mechanism underlying their comorbidity might be explained, at least in part, by shared genetics. To assess their potential genetic relationship, we performed a two-sample mendelian randomization (2SMR) analysis on results from public genome-wide association studies (GWAS). This analysis confirmed previously reported genetic pleiotropy between endometriosis and endometrial cancer. We present robust evidence supporting a causal genetic association between endometriosis and ovarian cancer, particularly with the clear cell and endometrioid subtypes. Our study also identified genetic variants that could explain those associations, opening the door to further functional experiments. Overall, this work demonstrates the value of genomic analyses to support epidemiological data, and to identify targets of relevance in multiple disorders. 相似文献
56.
Alexandra Calle Víctor Toribio María Yez-M Miguel ngel Ramírez 《International journal of molecular sciences》2021,22(11)
Embryonic implantation is a key step in the establishment of pregnancy. In the present work, we have carried out an in-depth proteomic analysis of the secretome (extracellular vesicles and soluble proteins) of two bovine blastocysts embryonic trophectoderm primary cultures (BBT), confirming different epithelial–mesenchymal transition stages in these cells. BBT-secretomes contain early pregnancy-related proteins and angiogenic proteins both as cargo in EVs and the soluble fraction. We have demonstrated the functional transfer of protein-containing secretome between embryonic trophectoderm and maternal MSC in vitro using two BBT primary cultures eight endometrial MSC (eMSC) and five peripheral blood MSC (pbMSC) lines. We observed that eMSC and pbMSC chemotax to both the soluble fraction and EVs of the BBT secretome. In addition, in a complementary direction, we found that the pattern of expression of implantation proteins in BBT-EVs changes depending on: (i) their epithelial–mesenchymal phenotype; (ii) as a result of the uptake of eMSC- or pbMSC-EV previously stimulated or not with embryonic signals (IFN-τ); (iii) because of the stimulation with the endometrial cytokines present in the uterine fluid in the peri-implantation period. 相似文献
57.
Bernhard Beckermann Jorge Bustamante Reinaldo Martínez-Cruz José M. Quesada 《Calcolo》2014,51(2):319-328
For a given $\theta \in (a,b)$ , we investigate the question whether there exists a positive quadrature formula with maximal degree of precision which has the prescribed abscissa $\theta $ plus possibly $a$ and/or $b$ , the endpoints of the interval of integration. This study relies on recent results on the location of roots of quasi-orthogonal polynomials. The above positive quadrature formulae are useful in studying problems in one-sided polynomial $L_1$ approximation. 相似文献
58.
Jorge González-Domínguez Osni A. Marques María J. Martín Juan Touriño 《The Journal of supercomputing》2014,70(2):816-829
This paper examines four different strategies, each one with its own data distribution, for implementing the parallel conjugate gradient (CG) method and how they impact communication and overall performance. Firstly, typical 1D and 2D distributions of the matrix involved in CG computations are considered. Then, a new 2D version of the CG method with asymmetric workload, based on leaving some threads idle during part of the computation to reduce communication, is proposed. The four strategies are independent of sparse storage schemes and are implemented using Unified Parallel C (UPC), a Partitioned Global Address Space (PGAS) language. The strategies are evaluated on two different platforms through a set of matrices that exhibit distinct sparse patterns, demonstrating that our asymmetric proposal outperforms the others except for one matrix on one platform. 相似文献
59.
60.
Enterotoxigenic <Emphasis Type="Italic">Escherichia coli</Emphasis> strains bind bovine milk gangliosides in a ceramide-dependent process 总被引:2,自引:0,他引:2
Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the
intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in
particular, several gangliosides. Because milk also contains gangliosides, these have been suggested to serve as ligands for
bacterial fimbriae and thus prevent the bacterial attachment to mucosa. The most relevant ETEC strains in calves, including
those with K99 and F41 fimbriae, were assayed to determine whether they are able to bind gangliosides isolated from several
stages of bovine lactation. Both GM3 and GD3, the main gangliosides of milk, were recognized by ETEC strains, although the
different fimbriae showed diverse levels of affinity. Unexpectedly, the adhesion to colostral gangliosides was considerably
weaker than that to gangliosides from the other stages of lactation. Because the carbohydrate moiety did not change and because
differences in the percentages of unsaturated FA and sphingosine between colostrum and other stages were observed, we conclude
that the differences in adhesion could be due to a different composition of the ganglioside caramide. 相似文献