Two-Dimensional Compact Third-Order Polynomial Reconstructions. Solving Nonconservative Hyperbolic Systems Using GPUs

We present a new kind of high-order reconstruction operator of polynomial type, which is used in combination with the scheme presented in Castro et al. (J. Sci. Comput. 39:67?C114, 2009) for solving nonconservative hyperbolic systems. The implementation of the scheme is carried out on Graphics Processing Units (GPUs), thus achieving a substantial improvement of the speedup with respect to normal CPUs. As an application, the two-dimensional shallow water equations with geometrical source term due to the bottom slope is considered.     

An ontological conceptualization approach for awareness in domain-independent collaborative modeling systems: Application to a model-driven development method

One of the most important aspects of collaborative systems is the concept of awareness, which refers to the perception and knowledge of the group and its activities. Support for the design and automatic development of awareness mechanisms within collaborative systems is hard to find. Furthermore, awareness conceptualizations are usually partial and differ greatly between the proposals of different authors. In response to these problems, we propose an awareness ontology that conceptualizes some of the most important aspects of awareness in a specific kind of system: collaborative systems for carrying out modeling activities. The awareness ontology brings together and extends a series of ontologies we have developed in the past. The ontology is prepared to better meet the specific implementation needs of a model-driven development approach. In order to validate the usefulness of this ontology, we relate its concepts to the awareness dimensions set out in Gutwin and Greenberg’s framework, and we apply the ontology to two systems presently in use.     

Measuring effective leaf area index, foliage profile, and stand height in New England forest stands using a full-waveform ground-based lidar

Effective leaf area index (LAI) retrievals from a scanning, ground-based, near-infrared (1064 nm) lidar that digitizes the full return waveform, the Echidna Validation Instrument (EVI), are in good agreement with those obtained from both hemispherical photography and the Li-Cor LAI-2000 Plant Canopy Analyzer. We conducted trials at 28 plots within six stands of hardwoods and conifers of varying height and stocking densities at Harvard Forest, Massachusetts, Bartlett Experimental Forest, New Hampshire, and Howland Experimental Forest, Maine, in July 2007. Effective LAI values retrieved by four methods, which ranged from 3.42 to 5.25 depending on the site and method, were not significantly different (β < 0.1 among four methods). The LAI values also matched published values well. Foliage profiles (leaf area with height) retrieved from the lidar scans, although not independently validated, were consistent with stand structure as observed and as measured by conventional methods. Canopy mean top height, as determined from the foliage profiles, deviated from mean RH100 values obtained from the Lidar Vegetation Imaging Sensor (LVIS) airborne large-footprint lidar system at 27 plots by − 0.91 m with RMSE = 2.04 m, documenting the ability of the EVI to retrieve stand height. The Echidna Validation Instrument is the first realization of the Echidna® lidar concept, devised by Australia's Commonwealth Scientific and Industrial Research Organization (CSIRO), for measuring forest structure using full-waveform, ground-based, scanning lidar.     

NDT measurements for the prediction of 28-day compressive strength

A procedure is proposed for the prediction of the compressive strength of concrete based on the use of NDT measurements. Prior to formwork removal, electrical resistivity can be assessed and based on an electrical resistivity versus compressive strength correlation curve, the strength of concrete can be estimated. This procedure ensures, for example, that the formwork is only removed when the concrete reaches a predefined compressive strength, dispensing with the calculation of concrete maturity. On the other hand, the whole surface of the concrete structure can be systematically measured so as to estimate 28-day compressive strengths and detect areas that could potentially not conform to the specified requirements. In this manner, a realistic overview of the in situ compressive strength of the whole structure can be obtained.The procedure proposed predicts the 28-day strength of concrete based on two models. The first model is based on an empirical equation used for the maturity assessment of concrete. The second model is based on a theoretical equation for the nucleation process of cement hydration. The effect of temperature and different curing conditions (concrete maturity) can be effectively taken into account by the conversion of all electrical resistivity measurement to the same referential. The models are used to estimate 28-day compressive strength based on the 7-day electrical resistivity measurements. The results show that the errors in estimation of the 28-day compressive strength are less than 22% and 10% for these models, respectively. If 28-day electrical resistivity measurements are used then the error in estimation is lower, circa 9% and 5% for these models, respectively.     

Carbon dioxide sorption and diffusion in poly(3‐hydroxybutyrate) and poly(3‐hydroxybutyrate‐CO‐3‐hydroxyvalerate)

CO₂ sorption and diffusion in poly(3‐hydroxybutyrate) and three poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) copolymers were investigated gravimetrically at temperatures from 25° to 50°C and pressures up to 1 atm. The sorption behavior proved to be linear for all the copolymers studied. An additional set of measurements performed in a pressure decay apparatus at 35°C showed that the linearity could be extrapolated to pressures up to 25 atm. The sorption results obtained from both techniques were in good agreement. The poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) sorption kinetics were increasingly non‐Fickian at the higher temperatures, thus preventing the calculation of diffusion coefficients above 35°C. Interestingly, this was not the case for poly(3‐hydroxybutyrate), and diffusion coefficients and permeabilities could be calculated at all of the investigated temperatures. The 35°C permeabilities were fairly low, which is attributed to the high degree of crystallinity of this polyester family. Finally, the poly(3‐hydroxybutyrate) barrier properties against CO₂ are successfully compared with those of some selected common thermoplastics. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 71: 2391–2399, 1999     

Cellular Crosstalk between Endothelial and Smooth Muscle Cells in Vascular Wall Remodeling

Pathological vascular wall remodeling refers to the structural and functional changes of the vessel wall that occur in response to injury that eventually leads to cardiovascular disease (CVD). Vessel wall are composed of two major primary cells types, endothelial cells (EC) and vascular smooth muscle cells (VSMCs). The physiological communications between these two cell types (EC–VSMCs) are crucial in the development of the vasculature and in the homeostasis of mature vessels. Moreover, aberrant EC–VSMCs communication has been associated to the promotor of various disease states including vascular wall remodeling. Paracrine regulations by bioactive molecules, communication via direct contact (junctions) or information transfer via extracellular vesicles or extracellular matrix are main crosstalk mechanisms. Identification of the nature of this EC–VSMCs crosstalk may offer strategies to develop new insights for prevention and treatment of disease that curse with vascular remodeling. Here, we will review the molecular mechanisms underlying the interplay between EC and VSMCs. Additionally, we highlight the potential applicable methodologies of the co-culture systems to identify cellular and molecular mechanisms involved in pathological vascular wall remodeling, opening questions about the future research directions.     

Tamoxifen Sensitizes Acute Lymphoblastic Leukemia Cells to Cannabidiol by Targeting Cyclophilin-D and Altering Mitochondrial Ca2+ Homeostasis

Cytotoxic effects of cannabidiol (CBD) and tamoxifen (TAM) have been observed in several cancer types. We have recently shown that CBD primarily targets mitochondria, inducing a stable mitochondrial permeability transition pore (mPTP) and, consequently, the death of acute lymphoblastic leukemia (T-ALL) cells. Mitochondria have also been documented among cellular targets for the TAM action. In the present study we have demonstrated a synergistic cytotoxic effect of TAM and CBD against T-ALL cells. By measuring the mitochondrial membrane potential (ΔΨm), mitochondrial calcium ([Ca²⁺]_m) and protein-ligand docking analysis we determined that TAM targets cyclophilin D (CypD) to inhibit mPTP formation. This results in a sustained [Ca²⁺]_m overload upon the consequent CBD administration. Thus, TAM acting on CypD sensitizes T-ALL to mitocans such as CBD by altering the mitochondrial Ca²⁺ homeostasis.     

Melanin Transfer in the Epidermis: The Pursuit of Skin Pigmentation Control Mechanisms

The mechanisms by which the pigment melanin is transferred from melanocytes and processed within keratinocytes to achieve skin pigmentation remain ill-characterized. Nevertheless, several models have emerged in the past decades to explain the transfer process. Here, we review the proposed models for melanin transfer in the skin epidermis, the available evidence supporting each one, and the recent observations in favor of the exo/phagocytosis and shed vesicles models. In order to reconcile the transfer models, we propose that different mechanisms could co-exist to sustain skin pigmentation under different conditions. We also discuss the limited knowledge about melanin processing within keratinocytes. Finally, we pinpoint new questions that ought to be addressed to solve the long-lasting quest for the understanding of how basal skin pigmentation is controlled. This knowledge will allow the emergence of new strategies to treat pigmentary disorders that cause a significant socio-economic burden to patients and healthcare systems worldwide and could also have relevant cosmetic applications.     

Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv

Biological therapies, such as recombinant proteins, are nowadays amongst the most promising approaches towards precision medicine. One of the most innovative methodologies currently available aimed at improving the production yield of recombinant proteins with minimization of costs relies on the combination of in silico studies to predict and deepen the understanding of the modified proteins with an experimental approach. The work described herein aims at the design and production of a biomimetic vector containing the single-chain variable domain fragment (scFv) of an anti-HER2 antibody fragment as a targeting motif fused with HIV gp41. Molecular modeling and docking studies were performed to develop the recombinant protein sequence. Subsequently, the DNA plasmid was produced and HEK-293T cells were transfected to evaluate the designed vector. The obtained results demonstrated that the plasmid construction is robust and can be expressed in the selected cell line. The multidisciplinary integrated in silico and experimental strategy adopted for the construction of a recombinant protein which can be used in HER2+-targeted therapy paves the way towards the production of other therapeutic proteins in a more cost-effective way.