Bead-based polymerase chain reaction on a microchip

We present a bead-based approach to microfluidic polymerase chain reaction (PCR), enabling fluorescent detection and sample conditioning in a single microchamber. Bead-based PCR, while not extensively investigated in microchip format, has been used in a variety of bioanalytical applications in recent years. We leverage the ability of bead-based PCR to accumulate fluorescent labels following DNA amplification to explore a novel DNA detection scheme on a microchip. The microchip uses an integrated microheater and temperature sensor for rapid control of thermal cycling temperatures, while the sample is held in a microchamber fabricated from (poly)dimethylsiloxane and coated with Parylene. The effects of key bead-based PCR parameters, including annealing temperature and concentration of microbeads in the reaction mixture, are studied to achieve optimized device sensitivity and detection time. The device is capable of detecting a synthetically prepared section of the Bordetella pertussis genome in as few as 10 temperature cycles with times as short as 15?min. We then demonstrate the use of the procedure in an integrated device; capturing, amplifying, detecting, and purifying template DNA in a single microfluidic chamber. These results show that this method is an effective method of DNA detection which is easily integrated in a microfluidic device to perform additional steps such as sample pre-conditioning.     

Design and implementation of a confidentiality and access control solution for publish/subscribe systems

The publish/subscribe model offers a loosely-coupled communication paradigm where applications interact indirectly and asynchronously. Publishers generate events that are sent to interested applications through a network of brokers. Subscribers express their interest by specifying filters that brokers can use for routing the events. Supporting confidentiality of messages being exchanged is still challenging. First of all, it is desirable that any scheme used for protecting the confidentiality of both the events and filters should not require publishers and subscribers to share secret keys. In fact, such a restriction is against the loose-coupling of the model. Moreover, such a scheme should not restrict the expressiveness of filters and should allow the broker to perform event filtering to route the events to the interested parties. Existing solutions do not fully address these issues. In this paper, we provide a novel scheme that supports (i) confidentiality for events and filters; (ii) allows publishers to express further constraints about who can access their events; (iii) filters that can express very complex constraints on events even if brokers are not able to access any information in clear on both events and filters; (iv) and, finally, it does not require publishers and subscribers to share keys. Furthermore, we show how we applied our scheme to a real-world e-health scenario, developed together with a hospital. We also describe the implementation of our solution in Java and the integration with an existing publish/subscribe system.     

InSAR validation based on GNSS measurements in Bucharest

This research aims to complex validate radar displacement products over Bucharest based on Global Navigation Satellite System or GNSS. Validation was represented by the comparison of the recent TerraSAR-X (TSX) displacement results with field GNSS data. For this purpose, 24 and 27 high-resolution images acquired from the TSX satellite between 2011 and 2014 were processed by Permanent Scatterer Inteferometry (PSI) and Small BASeline Subset (SBAS) – interferometric techniques using synthetic aperture radar (SAR) images. The images were selected from a larger pool of SAR images, after consulting weather databases, which helped eliminate the images acquired under adverse weather conditions. GNSS locations were cross-checked on high-resolution optical images and 21 ground points (including three permanent stations) were selected. All these points were (1) characterized by high coherence on radar images; (2) were on the ground; (3) were situated in large vacant spaces; and (4) far away from radar obstacles such as trees. A major challenge in achieving the research goal was to overcome the lack of permanent stations at the ground level within the area of Bucharest. Instead of permanent stations, our team designed and installed forced centring systems that remained stable over time, eliminating the instalment errors of GNSS receivers. The line-of-site (LOS) components derived from GNSS data have been compared with those of the SAR scatterers closest to the GNSS markers (never coinciding). For each location, the vertical reading was projected in the LOS of the SAR sensor. These readings were compared with the displacement values determined at the locations of permanent scatterers (PSs) within 100 m from the GNSS nodes, by eliminating all scatterers on buildings. The comparison has shown a good agreement.     

CPAS: the UK’s national machine learning-based hospital capacity planning system for COVID-19

Machine Learning - The coronavirus disease 2019 (COVID-19) global pandemic poses the threat of overwhelming healthcare systems with unprecedented demands for intensive care resources. Managing...     

Small networks of polarized splicing processors are universal

In this paper, we consider the computational power of a new variant of networks of splicing processors in which each processor as well as the data navigating throughout the network are now considered to be polarized. While the polarization of every processor is predefined (negative, neutral, positive), the polarization of data is dynamically computed by means of a valuation mapping. Consequently, the protocol of communication is naturally defined by means of this polarization. We show that networks of polarized splicing processors (NPSP) of size 2 are computationally complete, which immediately settles the question of designing computationally complete NPSPs of minimal size. With two more nodes we can simulate every nondeterministic Turing machine without increasing the time complexity. Particularly, we prove that NPSP of size 4 can accept all languages in NP in polynomial time. Furthermore, another computational model that is universal, namely the 2-tag system, can be simulated by NPSP of size 3 preserving the time complexity. All these results can be obtained with NPSPs with valuations in the set \(\{-1,0,1\}\) as well. We finally show that Turing machines can simulate a variant of NPSPs and discuss the time complexity of this simulation.     

Optical and Flame-Retardant Properties of a Series of Polyimides Containing Side Chained Bulky Phosphaphenanthrene Units

Among the multitude of polymers with carbon-based macromolecular architectures that easily ignite in certain applications where short circuits may occur, polyimide has evolved as a class of polymers with high thermal stability while exhibiting intrinsic flame retardancy at elevated temperatures via a char-forming mechanism. However, high amounts of aromatic rings in the macromolecular backbone are required for these results, which may affect other properties such as film-forming capacity or mechanical properties; thus, much work has been done to structurally derivatize or make hybrid polyimide systems. In this respect, flexible polyimide films (PI(1–4)) containing bulky 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO) units have been developed starting from commercial dianhydrides and an aromatic diamine containing two side chain bulky DOPO groups. The chemical structure of PI(1–4)) was characterized by ¹H NMR, ¹³C NMR and ³¹P NMR spectroscopy. The optical properties, including absorption and luminescence spectra of these polymers, were analyzed. All polyimides containing DOPO derivatives emitted blue light with an emission maxima in the range of 340–445 nm, in solvents such as N,N-dimethylformamide, N-methyl-2-pyrrolidone, chloroform, and N,N-dimethylacetamide, while green light emission (λ_em = 487 nm for PI-4) was evidenced in a thin-film state. The thermal decomposition mechanism and flame-retardant behavior of the resulting materials were investigated by pyrolysis-gas-chromatography spectrometry (Py-GC), scanning electron microscopy (SEM), EDX maps and FTIR spectroscopy. The residues resulting from the TGA experiments were examined by SEM microscopy images and FTIR spectra to understand the pyrolysis mechanism.     

The compatibility of the polystyrene/acrylonitrile-butadiene-styrene grafted copolymer system

Summary The study of polystyrene (PS)/acrylonitrile-butadiene-styrene (ABS) grafted copolymer/cyclohexanone or methylene chloride system compatibility both in concentrated solutions and solid state has been carried out by phase separation, viscosimetrical measurements and thermal behaviour.Having in view the obtained data it has been ascertained that the systems present different degrees of compatibility depending on the mixtures composition. At higher temperatures, the compatibility of the components is changed.     

Training,research, and technical assistance to improve the water resources management in Southern Italy — The experience of CSEI Catania

Water resources management may be improved through the activities of organizations devoted to practice-oriented research, continuing education, and technical assistance. The experience carried out in southern Italy by CSEI Catania during 13 years of activity is presented. Two main activities are discussed: the research and training program on regional pollution control plans and the development of a water data bank.     

Intraoperative Tumor Detection Using Pafolacianine

Cancer is a leading cause of death worldwide, with increasing numbers of new cases each year. For the vast majority of cancer patients, surgery is the most effective procedure for the complete removal of the malignant tissue. However, relapse due to the incomplete resection of the tumor occurs very often, as the surgeon must rely primarily on visual and tactile feedback. Intraoperative near-infrared imaging with pafolacianine is a newly developed technology designed for cancer detection during surgery, which has been proven to show excellent results in terms of safety and efficacy. Therefore, pafolacianine was approved by the U.S. Food and Drug Administration (FDA) on 29 November 2021, as an additional approach that can be used to identify malignant lesions and to ensure the total resection of the tumors in ovarian cancer patients. Currently, various studies have demonstrated the positive effects of pafolacianine’s use in a wide variety of other malignancies, with promising results expected in further research. This review focuses on the applications of the FDA-approved pafolacianine for the accurate intraoperative detection of malignant tissues. The cancer-targeting fluorescent ligands can shift the paradigm of surgical oncology by enabling the visualization of cancer lesions that are difficult to detect by inspection or palpation. The enhanced detection and removal of hard-to-detect cancer tissues during surgery will lead to remarkable outcomes for cancer patients and society, specifically by decreasing the cancer relapse rate, increasing the life expectancy and quality of life, and decreasing future rates of hospitalization, interventions, and costs.