In vivo synchrotron study of thallium speciation and compartmentation in Iberis intermedia

Thallium (TI) is a metal of great toxicological concern and its prevalence in the natural environment has steadily increased as a result of manufacturing and combustion practices. Due to its low natural abundance and increasing demand, TI is the fourth most expensive metal, thus, recovery and reuse could be a profitable endeavor. The hyperaccumulator Iberis intermedia was examined via in vivo micro-X-ray absorption near edge (micro-XANES) and micro-X-ray fluorescence (micro-XRF) spectroscopies to determine the speciation and distribution of TI within leaves of the plant. I. intermedia plants were cultivated under controlled conditions in 0, 10, and 20 mg TI kg(-1) soil leading to a shoot concentration of up to 13 430 mg TI kg(-1) dry weight plant mass during 10 weeks of growth. Live plant leaves were examined by micro-XANES and micro-XRF which determined aqueous TI(I) to be the model species distributed primarily throughout the vascular network. A direct relationship of vein size to TI concentration was observed. The high uptake of TI and high potential biomass of I. intermedia, combined with knowledge of TI speciation and compartmentation within the plant, are discussed in terms of accumulation/tolerance mechanisms, consequences for potential food chain contamination, and phytomining strategies to reclaim TI-contaminated soils, sediments, and waters.     

Low metal levels in emotionally disturbed children.

Investigated possible relationships of metal levels to childhood behavioral disorders. Hair-metal concentrations in 22 emotionally disturbed children (mean age 8.72 yrs) were compared to hair-metal levels in 25 control Ss (mean age 8.4 yrs) drawn from the general school population. Each S was also rated on the Walker Problem Behavior Identification Checklist (WPBIC). Disturbed Ss had significantly higher hair-lead levels. Correlations were run between hair-metal levels and WPBIC ratings for the 2 groups. Arsenic, cadmium, and aluminum levels correlated positively and significantly with the WPBIC total scale score for the disturbed group. A continuing reexamination of metal-poisoning concentrations is needed because metals levels previously thought harmless may be associated with behavioral impairments in children. (12 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Correcting automatic speech recognition captioning errors in real time

Lectures can be digitally recorded and replayed to provide multimedia revision material for students who attended the class and a substitute learning experience for students unable to attend. Deaf and hard of hearing people can find it difficult to follow speech through hearing alone or to take notes while they are lip-reading or watching a sign-language interpreter. Synchronising the speech with text captions can ensure deaf students are not disadvantaged and assist all learners to search for relevant specific parts of the multimedia recording by means of the synchronised text. Automatic speech recognition has been used to provide real-time captioning directly from lecturers’ speech in classrooms but it has proved difficult to obtain accuracy comparable to stenography. This paper describes the development, testing and evaluation of a system that enables editors to correct errors in the captions as they are created by automatic speech recognition and makes suggestions for future possible improvements.     

Nonlinear Soil–Abutment–Bridge Structure Interaction for Seismic Performance-Based Design

Current seismic design of bridges is based on a displacement performance philosophy using nonlinear static pushover analysis. This type of bridge design necessitates that the geotechnical engineer predict the resistance of the abutment backfill soils, which is inherently nonlinear with respect to the displacement between soil backfill and the bridge structure. This paper employs limit-equilibrium methods using mobilized logarithmic-spiral failure surfaces coupled with a modified hyperbolic soil stress–strain behavior (LSH model) to estimate abutment nonlinear force-displacement capacity as a function of wall displacement and soil backfill properties. The calculated force-displacement capacity is validated against the results from eight field experiments conducted on various typical structure backfills. Using LSH and experimental data, a simple hyperbolic force-displacement (HFD) equation is developed that can provide the same results using only the backfill soil stiffness and ultimate soil capacity. HFD is compatible with current CALTRANS practice in regard to the seismic design of bridge abutments. The LSH and HFD models are powerful and effective tools for practicing engineers to produce realistic bridge response for performance-based bridge design.     

Heterogeneous InSb quantum well transistors on silicon for ultra-high speed, low power logic applications

The heterogeneous integration of InSb quantum well transistors onto silicon substrates is investigated for the first time. 85 nm gate length FETs with f_T = 305 GHz at V_ds = 0.5 V and DC performance suitable for digital logic are demonstrated on material with a buffer just 1.8 mum thick. An initial step towards integrating InSb FETs with mainstream Si CMOS for high-speed energy-efficient logic applications has been achieved.     

Robust and accurate registration of 2-D electrophoresis gels using point-matching.

Point-matching is a widely applied image registration method and many algorithms have been developed. Registration of 2-D electrophoresis gels is an important problem in biological research that presents many of the technical difficulties that beset point-matching: large numbers of points with variable densities, large nonrigid transformations between point sets, paucity of structural information and large numbers of unmatchable points (outliers) in either set. In seeking the most suitable algorithm for gel registration we have evaluated a number of approaches for accuracy and robustness in the face of these difficulties. Using synthetic images we test combinations of three algorithm components: correspondence assignment, distance metrics and image transformation. We show that a version of the iterated closest point (ICP) algorithm using a non-Euclidean distance metric and a robust estimation of transform parameters provides best performance, equalling SoftAssign in the presence of moderate image distortion, and providing superior robustness against large distortions and high outlier proportions. From this evaluation we develop a gel registration algorithm based on robust ICP and a novel distance metric combining Euclidean, shape context and image-related features. We demonstrate the accuracy of gel matching using synthetic distortions of real gels and show that robust estimation of transform parameters using M-estimators can enforce inverse consistency, ensuring that matching results are independent of the order of the images.     

Porcelain Faucet Handle Failure Analysis

A porcelain faucet handle failed after years of use in a residential rental property. Determining the cause of failure was hampered by the fact that the failed handle was no longer available. The analysis included mechanical testing of exemplar faucet handles to measure the reasonable forces that could be applied by hand and the forces necessary to cause failure. Interface geometry between the porcelain handle and the interior metal nut was a factor. Finite Element Analysis facilitated the study. Results showed that if the faucet handle had sustained damage during a gasket repair, then the failure could be accounted for.     

Iron‐Oxide‐Based Nanovector for Tumor Targeted siRNA Delivery in an Orthotopic Hepatocellular Carcinoma Xenograft Mouse Model

Hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. Small interfering RNA (siRNA) holds promise as a new class of therapeutics for HCC, as it can achieve sequence‐specific gene knockdown with low cytotoxicity. However, the main challenge in the clinical application of siRNA lies in the lack of effective delivery approaches that need to be highly specific and thus incur low or no systemic toxicity. Here, a nonviral nanoparticle‐based gene carrier is presented that can specifically deliver siRNA to HCC. The nanovector (NP‐siRNA‐GPC3 Ab) is made of an iron oxide core coated with chitosan‐polyethylene glycol (PEG) grafted polyethyleneimine copolymer, which is further functionalized with siRNA and conjugated with a monoclonal antibody (Ab) against human glypican‐3 (GPC3) receptor highly expressed in HCC. A rat RH7777 HCC cell line that coexpresses human GPC3 and firefly luciferase (Luc) is established to evaluate the nanovector. The nanoparticle‐mediated delivery of siRNA against Luc effectively suppresses Luc expression in vitro without notable cytotoxicity. Significantly, NP‐siLuc‐GPC3 Ab administered intravenously in an orthotopic model of HCC is able to specifically bound to tumor and induce remarkable inhibition of Luc expression. The findings demonstrate the potential of using this nanovector for targeted delivery of therapeutic siRNA to HCC.