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941.
942.
H.R. Oliveira D.A.L. Lourenco Y. Masuda I. Misztal S. Tsuruta J. Jamrozik L.F. Brito F.F. Silva J.P. Cant F.S. Schenkel 《Journal of dairy science》2019,102(11):9995-10011
Estimating single nucleotide polymorphism (SNP) effects over time is essential to identify and validate candidate genes (or quantitative trait loci) associated with time-dependent variation of economically important traits and to better understand the underlying mechanisms of lactation biology. Therefore, in this study, we aimed to estimate time-dependent effects of SNP and identifying candidate genes associated with milk (MY), fat (FY), and protein (PY) yields, and somatic cell score (SCS) in the first 3 lactations of Canadian Ayrshire, Holstein, and Jersey breeds, as well as suggest their potential pattern of phenotypic effect over time. Random regression coefficients for the additive direct genetic effect were estimated for each animal using single-step genomic BLUP, based on 2 random regression models: one considering MY, FY, and PY in the first 3 lactations and the other considering SCS in the first 3 lactations. Thereafter, SNP solutions were obtained for random regression coefficients, which were used to estimate the SNP effects over time (from 5 to 305 d in lactation). The top 1% of SNP that showed a high magnitude of SNP effect in at least 1 d in lactation were selected as relevant SNP for further analyses of candidate genes, and clustered according to the trajectory of their SNP effects over time. The majority of SNP selected for MY, FY, and PY increased the magnitude of their effects over time, for all breeds. In contrast, for SCS, most selected SNP decreased the magnitude of their effects over time, especially for the Holstein and Jersey breeds. In general, we identified a different set of candidate genes for each breed, and similar genes were found across different lactations for the same trait in the same breed. For some of the candidate genes, the suggested pattern of phenotypic effect changed among lactations. Among the lactations, candidate genes (and their suggested phenotypic effect over time) identified for the second and third lactations were more similar to each other than for the first lactation. Well-known candidate genes with major effects on milk production traits presented different suggested patterns of phenotypic effect across breeds, traits, and lactations in which they were identified. The candidate genes identified in this study can be used as target genes in studies of gene expression. 相似文献
943.
Kabilan Sakthivel Hitendra Kumar Mohamed G. A. Mohamed Bahram Talebjedi Justin Shim Homayoun Najjaran Mina Hoorfar Keekyoung Kim 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(30)
Cells in vivo are constantly subjected to multiple microenvironmental mechanical stimuli that regulate cell function. Although 2D cell responses to the mechanical stimulation have been established, these methods lack relevance as physiological cell microenvironments are in 3D. Moreover, the existing platforms developed for studying the cell responses to mechanical cues in 3D either offer low‐throughput, involve complex fabrication, or do not allow combinatorial analysis of multiple cues. Considering this, a stretchable high‐throughput (HT) 3D cell microarray platform is presented that can apply dynamic mechanical strain to cells encapsulated in arrayed 3D microgels. The platform uses inkjet‐bioprinting technique for printing cell‐laden gelatin methacrylate (GelMA) microgel array on an elastic composite substrate that is periodically stretched. The developed platform is highly biocompatible and transfers the applied strain from the stretched substrate to the cells. The HT analysis is conducted to analyze cell mechano‐responses throughout the printed microgel array. Also, the combinatorial analysis of distinct cell behaviors is conducted for different GelMA microenvironmental stiffnesses in addition to the dynamic stretch. Considering its throughput and flexibility, the developed platform can readily be scaled up to introduce a wide range of microenvironmental cues and to screen the cell responses in a HT way. 相似文献
944.
945.
Emily E. F. Brown Reza Rezaei Taylor R. Jamieson Jaahnavi Dave Nikolas T. Martin Ragunath Singaravelu Mathieu J. F. Crupi Stephen Boulton Sarah Tucker Jessie Duong Joanna Poutou Adrian Pelin Hamed Yasavoli-Sharahi Zaid Taha Rozanne Arulanandam Abera Surendran Mina Ghahremani Bradley Austin Chantal Matar Jean-Simon Diallo John C. Bell Carolina S. Ilkow Taha Azad 《International journal of molecular sciences》2021,22(5)
Despite sequence similarity to SARS-CoV-1, SARS-CoV-2 has demonstrated greater widespread virulence and unique challenges to researchers aiming to study its pathogenicity in humans. The interaction of the viral receptor binding domain (RBD) with its main host cell receptor, angiotensin-converting enzyme 2 (ACE2), has emerged as a critical focal point for the development of anti-viral therapeutics and vaccines. In this study, we selectively identify and characterize the impact of mutating certain amino acid residues in the RBD of SARS-CoV-2 and in ACE2, by utilizing our recently developed NanoBiT technology-based biosensor as well as pseudotyped-virus infectivity assays. Specifically, we examine the mutational effects on RBD-ACE2 binding ability, efficacy of competitive inhibitors, as well as neutralizing antibody activity. We also look at the implications the mutations may have on virus transmissibility, host susceptibility, and the virus transmission path to humans. These critical determinants of virus–host interactions may provide more effective targets for ongoing vaccines, drug development, and potentially pave the way for determining the genetic variation underlying disease severity. 相似文献
946.
Hashemian Mina Razzazi Farbod Zarrabi Houman Moin Mohammad Shahram 《Wireless Personal Communications》2021,117(2):637-654
Wireless Personal Communications - One of the challenges faced by machine learning in human activity recognition systems is the different distributions of the training and test samples. Transfer... 相似文献
947.
Water Resources Management - Conjunctive use (CU) of surface water (SW) and groundwater (GW) implies the optimal operation of water resources to reduce the negative effects compared to when each of... 相似文献
948.
949.
Journal of Computer Virology and Hacking Techniques - This paper presents an approach to detect anomalies in process-aware information systems. This approach is based on process mining and uses... 相似文献
950.
Shunsuke Masuda Jun-ichi Tomioka Hiroaki Tamura Yohsuke Tamura 《International Journal of Hydrogen Energy》2021,46(23):12399-12406
We investigate an initial burst pressure and residual burst pressure at the end of life (EOL) of compressed hydrogen containers and report that a container damage caused by a drop test has a large influence on burst pressure. The container damage induced through hydraulic sequential tests is investigated using nondestructive evaluations to clarify a strength decreasing mechanism. An ultrasonic flaw detection analysis is conducted before and after the drop test and indicated that the damage occurred at the cylindrical and dome parts of the container after the drop test. An X-ray computed tomography imaging identifies a delamination inside laminated structure made of carbon fiber reinforced plastics (CFRP) layer, with some degree of delamination reaching the end boss of the container. Results suggest that a load profile fluctuates in the CFRP layer at the dome part and that a burst strength of the dome part decreases. 相似文献