Prognosis of breast cancer is associated with one-carbon metabolism related nutrients among Korean women

ABSTRACT: BACKGROUND: The 5-year survival rate for breast cancer among Korean women has increased steadily; however, breast cancer remains the leading cause of cancer mortality among women. One-carbon metabolism, which requires an adequate supply of methyl group donors and B vitamins, may affect the prognosis of breast cancer. This aim of this study was to investigate the associations of dietary intake of vitamin B2, vitamin B6 and folate before diagnosis on the prognosis of breast cancer. METHODS: We assessed the dietary intake using a food frequency questionnaire with 980 women who were newly diagnosed and histopathologically confirmed to have primary breast cancer from hospitals in Korea, and 141 disease progression events occurred. Cox's proportional hazard regression models were used to estimate the hazard ratio (HR) and 95% confidence interval (95% CI) adjusting for age, education, recruitment sites, TNM stage, hormone status, nuclear grade and total calorie. RESULTS: There was no significant association between any one-carbon metabolism related nutrients (vitamin B2, B6 and folate) and the progression of breast cancer overall. However, one-carbon metabolism related nutrients were associated with disease progression in breast cancer patients stratified by subtypes. In ER + and/or PR + breast cancers, no association was observed; however, in ER--/PR-- breast cancers, a high intake of vitamin B2 and folate statistically elevated the HR of breast cancer progression (HR = 2.28; 95% CI, 1.20-4.35, HR = 1.84; 95% CI, 1.02-3.32, respectively) compared to a low intake. This positive association between the ER/PR status and progression of the disease was profound when the nutrient intakes were categorized in a combined score (Pinteraction = 0.018). In ER--/PR-- breast cancers, high combined scores were associated with a significantly poor DFS compared to those belonging to the low score group (HR = 3.84; 95% CI, 1.70-8.71). CONCLUSIONS: In conclusion, our results suggest that one-carbon related nutrients have a role in the prognosis of breast cancer depending on the ER/PR status.     

Experimental realization of ‘universal homodyne tomography’ with a single local oscillator

We report preliminary experimental measurement of the twin-beam quantum state via optical homodyne tomography using a single local oscillator. The experiment is a realization of the recently reported ‘universal homodyne tomography’ technique. The results agree well with theoretical predictions and reveal the non-classical photon-number correlation between the signal and idler photons of the twin-beam state.     

Thermo-compression bonding of electrodes between FPCB and RPCB by using Pb-free solders

In this study, electrodes on a flexible printed circuit board (FPCB) and rigid PCB (RPCB) were bonded by a thermo-compression bonding. The surface finishes on the Cu electrodes of the FPCB and RPCB were organic-solderability-preservative and electroless Ni/immersion Au (ENIG), respectively. Pb-free Sn-3.0 Ag and Sn-3.0 Ag-0.5 Cu solders were used as interlayers. In order to determine the optimum bonding conditions, such as bonding force, time, and temperature, a 90° peel test of the FPCB-RPCB joint was conducted. The relationships between the bonding conditions and peel strength were investigated. We successfully accomplished the thermo-compression bonding of reliable FPCB-RPCB joints by using Sn-Ag and Sn-Ag-Cu interlayers. The thermo-compression bonding method is a stable and well-controlled process and can produce robust and reliable connections.     

Effects of supplement additives on anaerobic biogas production

Anaerobic digestion (AD) converts biomass to biogas. However, its performance is often affected by the nutrient condition of AD substrate. In this study, a few substrate supplements were selected to promote the biogas production; MgO, FeCl₃, and cellulase were selected based on the result from elemental analyses of the biomass. The potential impact of the additives on AD process was evaluated by performing a series of biochemical methane potential (BMP) tests. BMP reactors with the substrate with one of the selected additives (i.e., MgO of 380 mg Mg L^?1, FeCl₃ of 88 mg Fe L^?1 or cellulase of 25 mg L^?1) exhibited higher microbial activity; 5–15% more biogas production was observed, compared to the blank. Microbial community analysis showed that different additives resulted in proliferation of different microbial species. Therefore, it was decided to add the mixture of the three additives to the biomass. Addition of the mixed additive resulted in 22% more gas production.     

Analysis of ethanol in soy sauce using electronic nose for halal food certification

The purpose of this study was to analyze ethanol content in soy sauce using mass spectrometry (MS) with electronic nose (e-nose) to determine if MS e-nose can replace gas chromatographic analysis for halal certification. Gas chromatography–flame ionization detector (GC-FID), the standard method of ethanol content, was used to analyze 24 different kinds of soy sauce. Ethanol was detected from 13 soy sauce samples in the concentration range of 0.0004–1.7wt%. The MS e-nose data were analyzed by discriminant function analysis (DFA). Based on an addition method, the results were more than 96.6% accurate when the ethanol concentrations were greater than 0.5%. A high correlation between the first score of the DFA plot and the ethanol concentration was observed. Thus, mass spectrometry based on e-nose is an efficient method for determining ethanol as a primary screening tool for halal certification.     

A geometric approach to animating thin surface features in smoothed particle hydrodynamics water

We propose a geometric approach to animating thin surface features of smoothed particle hydrodynamics‐based water. Explicit interparticle connections are created among smoothed particle hydrodynamics particles to approximate the geometries of thin surfaces while addressing the issue of unresolved surface areas. The deformations measured on the connections actuate the animations of the surfaces by disconnecting the stretched and bent connections. The reconstruction of thin surfaces and the accuracy of the animation are improved by adding auxiliary particles over the connections via Poisson‐disk sampling. Copyright © 2014 John Wiley & Sons, Ltd.     

Structural and Piezoelectric Properties of (Na1−xKx)NbO3 Platelets and Their Application for Piezoelectric Nanogenerator

(Na_1?xK_x)NbO₃ (NKN) platelets synthesized at 600°C for 12 h have an Amm2 orthorhombic structure. However, the structure of NKN platelets synthesized at 500°C is a mixture of R3m rhombohedral and Amm2 orthorhombic structures. The formation of a rhombohedral structure is attributed to the presence of OH^? and H₂O defects in the NKN platelets. The piezoelectric strain constant (d₃₃) of NKN platelets synthesized at 600°C for 12 h is 100 pmV^?1, whereas that of NKN platelets synthesized at 500°C is lesser (50 pmV^?1) due to the presence of these defects. Piezoelectric nanogenerators (PNGs) are fabricated using composites consisting of NKN platelets and polydimethylsiloxane. A large output voltage of 25 V and output current of 2.7 μA were obtained for the PNG with NKN platelets synthesized at 600°C for 6 h. This PNG shows a high output electrical energy of 3.0 μW at an external load of 5.1 MΩ.     

Novel Role of Ghrelin Receptor in Gut Dysbiosis and Experimental Colitis in Aging

Chronic low-grade inflammation is a hallmark of aging, which is now coined as inflamm-aging. Inflamm-aging contributes to many age-associated diseases such as obesity, type 2 diabetes, cardiovascular disease, and inflammatory bowel disease (IBD). We have shown that gut hormone ghrelin, via its receptor growth hormone secretagogue receptor (GHS-R), regulates energy metabolism and inflammation in aging. Emerging evidence suggests that gut microbiome has a critical role in intestinal immunity of the host. To determine whether microbiome is an integral driving force of GHS-R mediated immune-metabolic homeostasis in aging, we assessed the gut microbiome profiles of young and old GHS-R global knockout (KO) mice. While young GHS-R KO mice showed marginal changes in Bacteroidetes and Firmicutes, aged GHS-R KO mice exhibited reduced Bacteroidetes and increased Firmicutes, featuring a disease-susceptible microbiome profile. To further study the role of GHS-R in intestinal inflammation in aging, we induced acute colitis in young and aged GHS-R KO mice using dextran sulfate sodium (DSS). The GHS-R KO mice showed more severe disease activity scores, higher proinflammatory cytokine expression, and decreased expression of tight junction markers. These results suggest that GHS-R plays an important role in microbiome homeostasis and gut inflammation during aging; GHS-R suppression exacerbates intestinal inflammation in aging and increases vulnerability to colitis. Collectively, our finding reveals for the first time that GHS-R is an important regulator of intestinal health in aging; targeting GHS-R may present a novel therapeutic strategy for prevention/treatment of aging leaky gut and inflammatory bowel disease.