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71.
Luz M. Ramos-Ponce Mireille Vega Georgina C. Sandoval-Fabián Edith Colunga-Urbina Nagamani Balagurusamy Francisco J. Rodriguez-Gonzalez Juan Carlos Contreras-Esquivel 《Food science and biotechnology》2010,19(3):683-689
A colorimetric method for quantitative measurement of free amino groups of water soluble chitin derivatives is described.
The method utilizes genipin as a natural and specific reagent for determining the concentration of free amino groups in samples
of water soluble chitin derivatives. The blue color adduct (complex) formed during genipin reaction with free amino groups
was measured at about 589 nm and Beer-Lambert’s law obeyed over the concentration range of 50 to 300 mg/L. Parameters of analytical
conditions were considered and kept constant during the experimental procedure. Highly acetylated water soluble chitin derivatives
can be differentiated from water soluble chitosan using this genipin method. The colorimetric method with genipin was proved
to be a rapid and efficient technique to determine the free amino groups in water soluble chitin derivatives. This method
can also be applied for the detection of the enzymatic activity of chitindeacetylase. 相似文献
72.
Mireille Ribière 《History of Photography》2013,37(4):286-292
Abstract The modest appearance of I Like to Eat Right on the Dirt: A Child's Journey Back in Space and Time (1989), by Danny Lyon belies its true portent.1 As an experimental photo story, developed from purely photographic models, this book opens up whole new horizons. 相似文献
73.
74.
Christian Gontrand Fengyuan Sun José Ricardo Cardenas-Valdez Yue Ma Carole Plossu Francis Calmon José Cruz Nuñez-Perez Jacques Verdier 《Microelectronics Journal》2014
3D Si integration seems a right way to go and compete with Moore?s law (more than Moore versus more Moore). However, it is still a long way to go. 相似文献
75.
Sofie Lust Barbara Vanhoecke Mireille Van Gele Jan Philippé Marc Bracke Fritz Offner 《Molecular nutrition & food research》2010,54(6):823-832
We explored the mechanism of cell death of the polymethoxyflavone tangeretin (TAN) in K562 breakpoint cluster region‐abelson murine leukemia (Bcr‐Abl+) cells. Flow cytometric analysis showed that TAN arrested the cells in the G2/M phase and stimulated an accumulation of the cells in the sub‐G0 phase. TAN‐induced cell death was evidenced by poly(ADP)‐ribose polymerase cleavage, DNA laddering fragmentation, activation of the caspase cascade and downregulation of the antiapoptotic proteins Mcl‐1 and Bcl‐xL. Pretreatment with the pancaspase inhibitor Z‐VAD‐FMK_blocked caspase activation and cell cycle arrest but did not inhibit apoptosis which suggest that other cell killing mechanisms like endoplasmic reticulum (ER)‐associated cell death pathways could be involved. We demonstrated that TAN‐induced apoptosis was preceded by a rapid activation of the proapoptotic arm of the unfolded protein response, namely PKR‐like ER kinase. This was accompanied by enhanced levels of glucose‐regulated protein of 78 kDa and of spliced X‐box binding protein 1. Furthermore, TAN sensitized K562 cells to the cell killing effects of imatinib via an apoptotic mechanism. In conclusion, our results suggest that TAN is able to induce apoptosis in Bcr‐Abl+ cells via cell cycle arrest and the induction of the unfolded protein response, and has synergistic cytotoxicity with imatinib. 相似文献
76.
Isotropic and anisotropic out-of-plane deformations induced by thin-film residual stress on thin cubic materials are studied. By transforming the compliance tensor, an analytical expression can be derived for the biaxial stiffness modulus for all directions in any given cubic crystal plane. A modified Stoney's equation, including both isotropic and anisotropic terms, can be formulated to predict the anisotropic out-of-plane deformation. The isotropic and anisotropic deformations are then described using the Zernike polynomials U21 and U22, respectively. Experimental results from (100) and (110) silicon wafers confirm the model by quantitatively comparing the changes in Z21 and Z22 coefficients due to thin-film stress. 相似文献
77.
A population-based study was carried out in 1996-2001 to provide the incidence and to identify the risk factors of severe traumatic brain injury (TBI) resulting from a road accident. The severe TBI was defined as an injury to the brain or the skull, excluding scalp injuries, with an abbreviated injury scale (AIS) severity score greater than 2. The severe TBI of 1238 patients were described. The annual incidence and mortality of severe TBI were, respectively, 13.7 per 100,000 and 5.3 per 100,000. The fatality rate increased from 20% in childhood to 71% over 75-year-old. Compared to restrained car occupants, the odds ratio for having a severe TBI was 18.1 (95% confidence interval, CI=12.8-25.5) for un-helmeted motorcyclists, 9.2 (95% CI=7.5-11.3) for pedestrians, 6.4 (95% CI=4.7-8.8) for un-helmeted cyclists, 3.9 (95% CI=3.1-4.8) for unrestrained car occupants and 2.8 (95% CI=2.2-3.5) for helmeted motorcyclists. Even after adjustment for several severity factors, male gender and age above 55 were both risk factors. Prevention programs aiming at improving the head protection should be promoted. The circumstances of the accident should be taken into account to predict a severe TBI. 相似文献
78.
Studies of lysozyme have played a major role over several decades in defining the general principles underlying protein structure, folding, and stability. Following the discovery some 10 years ago that two mutational variants of lysozyme are associated with systemic amyloidosis, these studies have been extended to investigate the mechanism of amyloid fibril formation. This Account describes our present knowledge of lysozyme folding and misfolding, and how the latter can give rise to amyloid disease. It also discusses the significance of these studies for our general understanding of normal and aberrant protein folding in the context of human health and disease. 相似文献
79.
Nathalie Nallet Marlène Bernard Mireille Chiron 《Accident; analysis and prevention》2008,40(6):1836-1843
This paper sets out to describe the drivers attending driving licence points recovery courses (PRC), in order to modify the psychologist’s approach during the sessions.The paper describes a questionnaire-based survey of a representative sample of 853 drivers taking a “course designed to raise awareness about the causes and consequences of road traffic accidents”. The data are analyzed in part by applying a multiple correspondence analysis.Among those taking part in PRC, recognizing whether they commit violations or not seems to be independent from the perceived probability of sanctioning and the perceived danger. The group of course-takers is divided into two main clusters (“offenders” and “victims”) which require a completely different approach on the part of the training staff. 相似文献
80.
Francesca Terenziani Claudine Katan Ekaterina Badaeva Sergei Tretiak Mireille Blanchard‐Desce 《Advanced materials (Deerfield Beach, Fla.)》2008,20(24):4641-4678
Functional organic materials with enhanced two‐photon absorption lead to new technologies in the fields of chemistry, biology, and photonics. In this article we review experimental and theoretical methodologies allowing detailed investigation and analysis of two‐photon absorption properties of organic chromophores. This includes femtosecond two‐photon excited fluorescence experimental setups and quantum‐chemical methodologies based on time‐dependent density functional theory. We thoroughly analyze physical phenomena and trends leading to large two‐photon absorption responses of a few series of model chromophores focusing on the effects of symmetric and asymmetric donor/acceptor substitution and branching. 相似文献