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81.
82.
Chondrogenic differentiation of human adipose mesenchimal stem cells: Influence of a biomimetic gelatin genipin crosslinked porous scaffold
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Stefano Focaroli Gabriella Teti Viviana Salvatore Sandra Durante Monica Mattioli Belmonte Roberto Giardino Antonio Mazzotti Adriana Bigi Mirella Falconi 《Microscopy research and technique》2014,77(11):928-934
Human adipose derived stem cells have shown chondrogenic differentiation potential in cartilage tissue engineering in combination with biomimetic materials. In this study, the chondrogenic potential of a porous gelatin based scaffold genipin (GNP) crosslinked was investigated in human mesenchymal stem cells obtained from adipose tissue. Cells were cultured up to 4 weeks on the scaffold and on monolayer, MTT assay was performed to evaluate cell viability, light, and transmission electron microscopy were carried out to demonstrate cell proliferation, scaffold adhesion, and cell colonization inside the porous architecture of the biomaterial. The expression of chondrogenic markers such as SOX9, collagen type II, aggregan, and versican was investigated by Real Time PCR. Results showed an high cell viability, adhesion, and colonization of the scaffold. Real Time PCR data demonstrated an upregulation of all the chondrogenic markers analyzed. In conclusion, 3D gelatin GNP crosslinked porous scaffold provides an improved environment for chondrogenic differentiation of stem cells compared with cell monolayer culture system. Microsc. Res. Tech. 77:928–934, 2014. © 2014 Wiley Periodicals, Inc. 相似文献
83.
Guillaume Vidon Pia Dally Mirella Al-Katrib Daniel Ory Minjin Kim Etienne Soret Eva Rangayen Marie Legrand Alexandre Blaizot Philip Schulz Jean-Baptiste Puel Daniel Suchet Jean-François Guillemoles Arnaud Etcheberry Muriel Bouttemy Stefania Cacovich 《Advanced functional materials》2023,33(45):2304730
Understanding the effects of X-rays on halide perovskite thin films is critical for accurate and reliable characterization of this class of materials, as well as their use in detection systems. In this study, advanced optical imaging techniques are employed, both spectrally and temporally resolved, coupled with chemical characterizations to obtain a comprehensive picture of the degradation mechanism occurring in the material during photoemission spectroscopy measurements. Two main degradation pathways are identified through the use of local correlative physico-chemical analysis. The first one, at low X-Ray fluence, shows minor changes of the surface chemistry and composition associated with the formation of electronic defects. Moreover, a second degradation route occurring at higher fluence leads to the evaporation of the organic cations and the formation of an iodine-poor perovskite. Based on the local variation of the optoelectronic properties, a kinetic model describing the different mechanisms is proposed. These findings provide valuable insight on the impact of X-rays on the perovskite layers during investigations using X-ray based techniques. More generally, a deep understanding of the interaction mechanism of X-rays with perovskite thin films is essential for the development of perovskite-based X-ray detectors and solar for space applications. 相似文献
84.
Cecilia Anselmi Marisanna Centini Mirella Scotton Alessandro Sega P. Dapporto P. Paoli 《Advanced Synthesis \u0026amp; Catalysis》1992,334(2):126-130
The solid state conformation of the title compound was determined. Conformational analysis based on molecular modelling mechanics allowed the calculation of the energy of the most stable conformers and their comparison with X-ray conformation. 相似文献