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801.
Iwona Bernacka‐Wojcik Miriam Huerta Klas Tybrandt Michal Karady Mohammad Yusuf Mulla David J. Poxson Erik O. Gabrielsson Karin Ljung Daniel T. Simon Magnus Berggren Eleni Stavrinidou 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(43)
Electronic control of biological processes with bioelectronic devices holds promise for sophisticated regulation of physiology, for gaining fundamental understanding of biological systems, providing new therapeutic solutions, and digitally mediating adaptations of organisms to external factors. The organic electronic ion pump (OEIP) provides a unique means for electronically‐controlled, flow‐free delivery of ions, and biomolecules at cellular scale. Here, a miniaturized OEIP device based on glass capillary fibers (c‐OEIP) is implanted in a biological organism. The capillary form factor at the sub‐100 µm scale of the device enables it to be implanted in soft tissue, while its hyperbranched polyelectrolyte channel and addressing protocol allows efficient delivery of a large aromatic molecule. In the first example of an implantable bioelectronic device in plants, the c‐OEIP readily penetrates the leaf of an intact tobacco plant with no significant wound response (evaluated up to 24 h) and effectively delivers the hormone abscisic acid (ABA) into the leaf apoplast. OEIP‐mediated delivery of ABA, the phytohormone that regulates plant's tolerance to stress, induces closure of stomata, the microscopic pores in leaf's epidermis that play a vital role in photosynthesis and transpiration. Efficient and localized ABA delivery reveals previously unreported kinetics of ABA‐induced signal propagation. 相似文献
802.
Carolina Fonseca Couto Wagner Guadagnin Moravia Miriam Cristina Santos Amaral 《Clean Technologies and Environmental Policy》2017,19(8):2057-2073
This study investigates the application of a hybrid microfiltration–nanofiltration (MF–NF) process for textile wastewater reclamation. Indigo blue dye was efficiently retained by an MF membrane, allowing its recovery from the concentrated stream. NF technology was successfully applied to polish textile effluent. The NF membrane was evaluated under different transmembrane pressure (8–15 bars), crossflow velocities (0.21–0.84 cm s?1), pH (7–11), and feed temperature (20–40 °C). The best NF performance was provided at a pressure of 12 bar and a crossflow rate of 0.63 cm s?1. The NF performance (in terms of COD, conductivity, colour, and nitrogen removal) was not influenced by pH; however, higher feed pH values resulted in increased membrane fouling. The principal cause of flux decline was due to concentration polarization. Membrane chemical cleaning was sufficient to regain the initial permeability. The NF permeate met the quality requirements for all water demands within the textile industry, while the NF concentrate could be used to wash equipment, print work screens, print paste containers, and floors. The total capital cost (CapEx) of the MF–NF system was estimated at 58,362.50 US dollars and the total operational cost (OpEx) at 0.31 US dollars per cubic metre of effluent. 相似文献
803.
Bar Israeli Daniela S. Strugach Sigal Gelkop Shir Weber Dor S. Gozlan Miriam Amiram 《Advanced functional materials》2021,31(44):2011276
A general and versatile technology to engineer light-responsive protein-based biomaterials can enable the manipulation and interrogation of proteins, pathways, and cells, and it will assist the design of “smart” light-responsive biomaterials. This study reports the evolution of chromosomal aminoacyl-tRNA synthetases (aaRSs) for azobenzene-bearing unnatural amino acids (uAAs) with up to ≈40-fold increased protein production and improved fidelity, as compared with a previously described aaRS. The evolved translation systems enable efficient and accurate incorporation of up to 10 instances of the various light-responsive uAAs in elastin-like polypeptides (ELPs). Azobenzene-containing ELPs are capable of isothermal, reversible, light-mediated soluble-to-insoluble phase transition, with up to a 12 °C difference in the ELP transition temperature upon cis-to-trans azobenzene isomerization. Furthermore, the incorporation of azobenzene-uAAs in ELP diblock-copolymers enables the creation of light-responsive self-assembled nanostructures. Finally, light-responsive resilin-inspired polymers are also generated by multi-site azobenzene-incorporation. The translation machinery evolved in this study can be used for the multi-site incorporation of azobenzene moieties at the polypeptide level and constitute a universal methodology for the design of light-responsive proteins and additional families of protein-based biomaterials with customized and tunable light-responsive behavior. 相似文献
804.
Zhi Li Joachim F. R. Van Guyse Victor R. de la Rosa Hans Van Gorp Peter Walke Miriam C. Rodríguez Gonzlez Hiroshi Uji‐i Richard Hoogenboom Steven De Feyter Stijn F. L. Mertens 《Advanced functional materials》2019,29(36)
The modification of solid surfaces with supramolecular hosts is a powerful method to tailor interfacial properties and confer chemical selectivity, but often involves multistep protocols that hinder facile upscaling. Here, the one‐step covalent modification of highly oriented pyrolytic graphite (HOPG) with a β‐cyclodextrin (β‐CD) derivative, which efficiently forms inclusion complexes with hydrophobic guests of suitable size, is demonstrated. The grafted β‐CD‐HOPG surface is investigated toward electrochemical detection of ferrocene and dopamine. The enrichment of the analytes at the electrode surface, through inclusion in β‐CD, leads to an enhanced electrochemical response and an improved detection limit. Furthermore, the modified β‐CD‐HOPG electrode discriminates analytes that form host–guest complexes with β‐CD against a 100‐fold higher background of electroactive substances that do not. Atomic force microscopy, scanning tunneling microscopy, and Raman spectroscopy confirm the covalent nature of the modification and reveal high stability toward solvent rinsing, ultrasonication, and temperatures up to 140 °C. The one‐step covalent modification therefore holds substantial promise for the routine production of inexpensive, yet robust and highly performant electrochemical sensors. Beyond electrochemical sensor development, our strategy is valuable to prepare materials where accurate spatial positioning of functional units and efficient current collection are crucial, e.g. in photoelectrodes or electrocatalysts. 相似文献
805.
Understanding culinary innovation as relational: Insights from Tarde's relational sociology
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Miriam Feuls 《Creativity & Innovation Management》2018,27(2):161-168
This article argues that a relational view of innovation opens up new perspectives of examining and explaining how novelty develops in creative industries. Although many researchers have given time to this topic, a theoretically grounded concept of relational innovation remains undeveloped within the literature. To address this issue, I set out to offer a framework informed by Gabriel Tarde's relational sociology, by re‐interpreting this sociology with regard to practice theory. By applying this framework in an empirical study of haute cuisine, I identify three processes of innovating at varying degrees of novelty (repeating, adapting, and differentiating). By relating those processes in the form of practices‐nets, I show that innovating is not a linear development process, but that a culinary innovation emerges in between relations of everyday practices that define and transform its value. I hope, in this way, to contribute to a more complex and subtle understanding of culinary innovation as relational. 相似文献
806.
Francisco Méndez-Martínez Miriam J. Venegas Heriberto Pfeiffer 《International Journal of Applied Ceramic Technology》2007,4(6):564-570
This work presents the effect of zinc oxide (ZnO) ceramics, doped with different percentages of vanadium trioxide (V2 O3 ) and vanadium pentoxide (V2 O5 ). Samples were analyzed by X-ray diffraction and scanning electron microscopy. The addition of V2 O3 or V2 O5 produced changes in the composition and morphology of the pellets. In both cases, different zinc vanadates were detected as secondary phases: α-Zn3 (VO4 )2 and Zn4 V2 O9 . Furthermore, when the vanadium concentration was equal to or higher than 3 wt%, the presence of filaments was detected on the surface of the pellets. These filaments were produced due to vanadium segregation. However, this effect was only observed at the surface of the pellets. On the bulk, the filaments were not observed. Instead, vanadium was found at the interfaces between the ZnO grains and at triple points, as it could be expected. 相似文献
807.
Samantha Salomão Caramori Flaviana Naves de Faria Miriam Pereira Viana Kátia Flávia Fernandes Luiz Bezerra Carvalho 《Materials science & engineering. C, Materials for biological applications》2011,31(2):252-257
Discs of polyvinyl alcohol cross-linked with glutaraldehyde (PVAG) were synthesized and covered with polyaniline activated with glutaraldehyde (PANIG). Trypsin was covalently immobilized on this composite yielding a preparation containing 21.1 units per disc. The FT-IR spectra of the discs showed bands of PVA (3300 cm?1, 2930 cm?1 and 1440 cm?1) and PANI (1594 cm?1 and 1100 cm?1). The best immobilization conditions were: trypsin concentration at 0.2 mg mL?1, pH 7.6 and 60 min of incubation, similar to polyaniline–trypsin systems reported in the literature. The PVAG–PANIG–trypsin derivative showed an optimal pH and an optimal temperature of 7.0 and 35 °C, respectively. Hydrolysis of casein showed variations in the size of the products, revealing differences between the immobilized enzyme and the mechanism catalyzed by the native enzyme. 相似文献
808.
Gláucia S. Vieira Leila M. Pereira Miriam D. Hubinger 《International Journal of Food Science & Technology》2012,47(1):132-140
Response surface methodology was used to assess the effects of osmotic solution concentration (40–60°Brix), process temperature (20–40 °C) and vacuum pulse application time (0–20 min) at 100 mbar on water loss (WL), weight reduction (WR), solid gain (SG), water activity (aw), colour parameters and mechanical properties of guava slices. Optimal process conditions were determined through the desirability function approach and quality characteristics of osmotically dehydrated guavas were analysed. Only models obtained for WL, WR and aw were suitable to describe the experimental data. The desirability function showed that optimal conditions for osmotic dehydration of guavas were: osmotic solution concentration at 60°Brix, process temperature at 32 °C and 20 min of vacuum pulse application. Under optimal conditions, colour and mechanical properties of treated guavas were similar to fresh fruit, presenting WL of 29.01 g/100 g, WR of 25.91 g/100 g, SG of 3.10 g/100 g and aw of 0.979. 相似文献
809.
Javier R. Arballo Ruth R. Bambicha Laura A. Campañone Miriam E. Agnelli Rodolfo H. Mascheroni 《International Journal of Food Science & Technology》2012,47(2):306-314
Mass transfer kinetics and optimisation of osmotic dehydration (OD) of fruits and vegetables with diverse structures were studied. Different concentrations of sucrose (20–60 °Brix) and process times (0–24 h) were used. Magee’s model was appropriate for predicting water loss (WL), while Azuara’s model fitted well solids gain (SG) data and represented more accurately the evolution of the complete process close to equilibrium. Polynomial equations for each kinetic variable [WL, SG and weight reduction (WR) – for pumpkin, kiwi and pear] using multiple linear regression were fitted for a selected range of experimental data (30–240 min, 20–60 °Brix). A complete solution algorithm for desirability function was coded in Matlab® 7.2 (Mathworks, Natick, MA, USA) with the aim to optimise osmotic dehydration process in terms of WL, SG and WR; optimal conditions were found for each fruit. Besides, an optimal common zone was identified for OD corresponding to process time from 114 to 240 min and sucrose concentration from 54 to 60 °Brix. 相似文献
810.
Ana Clarissa dos Santos Pires Nilda de Fátima Ferreira Soares Luis Henrique Mendes da Silva Nélio José de Andrade Miriam Fontes Araujo Silveira Antônio Fernandes de Carvalho 《Food and Bioprocess Technology》2010,3(2):172-181
Biosensors offer the potential for real-time pathogen detection. Polydiacetylene (PDA) is an ideal choice for use as a sensor
due to its unique optical properties. PDA molecules can form thin films or vesicles that change color from deep blue to red
in response to different stimuli, like temperature, pH, and the presence of biological molecules. PDA films and vesicles have
been proven to be promising devices for the detection of bacteria and bacterial toxins. Langmuir troughs (for films) and microcalorimetry
(for vesicles) are among the many techniques used to characterize PDA films and vesicles. In order to enable more applications
of PDA films, it is imperative to elucidate and gain a deeper understanding of the molecular interactions associated with
their color change. In addition, it is necessary to transfer the PDA film onto a solid support like plastic in order to incorporate
PDA biosensors into an intelligent packaging system. Through the use of such a system, consumers can monitor food quality,
thereby preventing foodborne outbreaks. In this review, we discuss the formation of PDA films and vesicles, their characteristics,
and their potential applications as biosensors. The possibility to incorporate PDA biosensors into an intelligent packaging
system to be used in the food industry is also explored. 相似文献