Proteins Marking the Sequence of Genotoxic Signaling from Irradiated Mesenchymal Stromal Cells to CD34+ Cells

Non-targeted effects (NTE) of ionizing radiation may initiate myeloid neoplasms (MN). Here, protein mediators (I) in irradiated human mesenchymal stromal cells (MSC) as the NTE source, (II) in MSC conditioned supernatant and (III) in human bone marrow CD34+ cells undergoing genotoxic NTE were investigated. Healthy sublethal irradiated MSC showed significantly increased levels of reactive oxygen species. These cells responded by increasing intracellular abundance of proteins involved in proteasomal degradation, protein translation, cytoskeleton dynamics, nucleocytoplasmic shuttling, and those with antioxidant activity. Among the increased proteins were THY1 and GNA11/14, which are signaling proteins with hitherto unknown functions in the radiation response and NTE. In the corresponding MSC conditioned medium, the three chaperones GRP78, CALR, and PDIA3 were increased. Together with GPI, these were the only four altered proteins, which were associated with the observed genotoxic NTE. Healthy CD34+ cells cultured in MSC conditioned medium suffered from more than a six-fold increase in γH2AX focal staining, indicative for DNA double-strand breaks, as well as numerical and structural chromosomal aberrations within three days. At this stage, five proteins were altered, among them IQGAP1, HMGB1, and PA2G4, which are involved in malign development. In summary, our data provide novel insights into three sequential steps of genotoxic signaling from irradiated MSC to CD34+ cells, implicating that induced NTE might initiate the development of MN.     

pHEMA: An Overview for Biomedical Applications

Poly(2-hydroxyethyl methacrylate) (pHEMA) as a biomaterial with excellent biocompatibility and cytocompatibility elicits a minimal immunological response from host tissue making it desirable for different biomedical applications. This article seeks to provide an in-depth overview of the properties and biomedical applications of pHEMA for bone tissue regeneration, wound healing, cancer therapy (stimuli and non-stimuli responsive systems), and ophthalmic applications (contact lenses and ocular drug delivery). As this polymer has been widely applied in ophthalmic applications, a specific consideration has been devoted to this field. Pure pHEMA does not possess antimicrobial properties and the site where the biomedical device is employed may be susceptible to microbial infections. Therefore, antimicrobial strategies such as the use of silver nanoparticles, antibiotics, and antimicrobial agents can be utilized to protect against infections. Therefore, the antimicrobial strategies besides the drug delivery applications of pHEMA were covered. With continuous research and advancement in science and technology, the outlook of pHEMA is promising as it will most certainly be utilized in more biomedical applications in the near future. The aim of this review was to bring together state-of-the-art research on pHEMA and their applications.     

Thermogravimetric analysis and kinetic study of heavy oil pyrolysis

Pyrolysis, so-called devolatilization, is one of the first steps of all thermochemical processes occurring in an inert atmosphere. The authors discuss the main kinetic features of heavy oil pyrolysis, on the basis of the data derived m from a TGA analysis and by using a kinetic model. The samples were heated over a range of temperature from 400 K to 430°C at various heating rates between 10 and 80°C/min. Experimental results showed that the effect of time is considerable in the case of tar conversion, compared to char and gases.     

An investigation on the role of ytterbium in ytterbium promoted γ-alumina-supported nickel catalysts for dry reforming of methane

Addition of low quantities of ytterbium to sol–gel prepared Ni/γ-Al₂O₃ catalysts has been shown to lead to significant increases in catalytic activity and long term stability in the catalytic conversion of CO₂ and CH₄ into syngas (H₂ and CO). The role of ytterbium in these catalysts was investigated in this study through detailed investigations on the structure and composition of ytterbium promoted Ni/γ-Al₂O₃ catalysts using the following techniques: synchrotron X-ray diffraction, X-ray Photoemission Spectroscopy, Transmission Electron Microscopy, Scanning Electron Microscopy/Energy Dispersive X-ray analysis, Temperature Programmed Reduction techniques and N₂ adsorption–desorption isotherms. The results obtained indicated that ytterbium, at small quantities (up to 2 wt%), interacted strongly with the support which in turn altered the interaction between nickel and the support (most notably it was found to completely inhibit the formation of NiAl₂O₄). This decreased interaction between Ni and the support also led to a higher quantity of Ni being present in the catalyst in the form of Ni.     

BOUNDARY ELEMENT ANALYSIS OF GREEN AND LINDSAY THEORY UNDER THERMAL AND MECHANICAL SHOCK IN A FINITE DOMAIN

A boundary element formulation based on the Laplace transform method is developed for transient coupled thermoelasticity problems with relaxation times in a two-dimensional finite domain. The dynamic thermoelastic model of Green and Lindsay is selected for the present study. The Laplace transform method is applied to the time domain, and the resulting equations in the transformed field are discretized using the boundary element method. The nodal dimensionless temperature and displacements in the transformed domain are inverted to obtain the actual physical quantities using the numerical inversion of the Laplace transform method. This work considers the Green and Lindsay theory of thermoelasticity with the thermal and mechanical loading in a finite domain. The creation and propagation of elastic and thermoelastic waves in a finite domain and their effects on each other are investigated. Different relaxation times are chosen to briefly illustrate the events that take place in GL theory. Details of the formulation and numerical implementation are also presented.     

A review of investigations on liquid fuel combustion in porous inert media

Utilization of a porous medium for combustion of liquid fuels is proved to be a promising approach for future applications. The porous medium burner for liquid fuels is more advantageous than the conventional open spray flame burner for several reasons; these include enhanced evaporation of droplet spray owing to regenerative combustion characteristics, low emission of pollutants, high combustion intensity with moderate turn-down ratio and compactness. This article provides a comprehensive picture of the global scenario of research and developments in combustion of liquid fuels within a porous medium that enable a researcher to determine the direction of further investigation. Accordingly, a glossary of the important terminology, the modeling approach, advances in numerical and experimental works and applications are included. The papers published in standard journals are reviewed and summarized with relevant comments and suggestions for future work.     

Watershed characteristics, land use and fabric: The application of remote sensing and geographical information systems

Integrated watershed assessment, especially relying on remote sensing (RS), is a newly established procedure in developing countries. It is proving to be a major component in river‐basin environmental management. The recurrence of environmental problems in the Akkar El Kabir River watershed, as well as the lack of proper data on sources and sinks of pollutants, and the extent of human interference, led to the current study. Advanced geoinformation tools, such as RS and geographical information systems (GIS), prove to be a valuable asset in securing data on the fabric of the Akkar watershed in relation to its natural setting and anthropic interference. This is particularly true in the current study as the river constitutes the boundary between Lebanon and Syria. Remote sensing captures the watershed characteristics and land use on both sides without constraints. The natural fabric includes geology, drainage, hydrogeology, forest and soil. The anthropic fabric includes settlements, utilities, roads, agriculture and land use. If it were not for geoinformation techniques, the task of securing such data would be difficult. Also, these techniques show the impact of malpractices from excessive human interference that result in degradation of land and water quality. Changes in the watershed, such as environmental deterioration, are observed as water pollution, soil erosion, forest decline and socioeconomic imbalance. Obviously, this is the outcome of malpractices in a multisectorial system. A major challenge for RS and GIS is to quantify, model and predict, if possible, the extent of these changes. Remote sensing inherently captures the impact of interaction between nature and human beings. Detection of change is a major indicator that RS can contribute to the evaluation of the state of the environment. The application of it on this watershed reveals that significant changes have occurred over the last 10–15 years, most of which are anthropic.     

Improving the Pressurized Flushing Efficiency in Reservoirs: an Experimental Study

Sediment flushing in many reservoirs of the world is accomplished with low efficiency. In this study, a new configuration was proposed for reservoir bottom outlet to increase the pressurized flushing efficiency. In the new configuration, a projecting semi-circular structure was connected to the upstream edge of bottom outlet. It was observed that by employing the projecting bottom outlet, the sediment removal efficiency increased significantly compared to the flushing via typical bottom outlet. In the case of new-configuration bottom outlet with L _sc /D _outlet  = 5.26 and D _sc /D _outlet  = 1.32, the dimensionless length, width and depth of flushing cone increased 280%, 45% and 14%, respectively, compared to the reference test. The proposed structure can ensure the sustainable use of reservoirs.     

Phosphorus and nitrogen in the waters of the El-Kabir River watershed in Syria and Lebanon

In order to assess the quality of the waters of the El‐Kabir River, which forms the border between Lebanon and Syria, water samples were collected for phosphorus (P) and nitrogen analyses at 39 sample stations in the river watershed (18 in Syria, 21 in Lebanon). These samples were collected on the main stem and three major tributaries (Nahr al‐Arous and Nahr Nasrive in Syria, Chadra River in Lebanon). Three major springs also were sampled. The sampling was carried out in September 2001, and January, April and August 2002. Nutrient analyses were carried out on samples taken in September 2001, January and April 2002 in Syria, and in September 2001 and August 2002 in Lebanon. The P concentrations were extremely high throughout the watershed, as were the ammonia‐nitrogen and nitrate‐nitrogen concentrations, indicating extensive pollution. Although the nitrite‐nitrogen concentration was relatively low, it is at the upper end of what might be considered normal, thereby perhaps being indicative of some anthropogenic sources. The spring waters were found to be polluted by nutrients. The nutrient sources contributing to the river pollution were mainly from piped, direct sewage discharges from the many settlements throughout the basin. These were supplemented by diffuse sources directly from agricultural fertilizer use and from the indiscriminate disposal of solid wastes into the river and on the stream banks and lands adjacent to the roads of the watershed.