Notes on integral performance indices in fractional-order control systems

Integral performance indices as quantitative measures of the performance of a system are commonly used to evaluate the performance of designed control systems. In this paper, it is pointed out that due to existence of non-exponential modes in the step response of a fractional-order control system having zero steady state error, integral performance indices of such a system may be infinite. According to this point, some simple conditions are derived to guarantee the finiteness of different integral performance indices in a class of fractional-order control systems. Finally, some numerical examples are presented to show the applicability of the analytical achievements of the paper.     

N ‐acetylcysteine–PLGA nano‐conjugate: effects on cellular toxicity and uptake of gadopentate dimeglumine

Gadolinium as a contrast agent in MRI technique combined with DTPA causes contrast induced nephropathy (CIN) and nephrogenic systemic fibrosis (NSF) which can reduce by usage of antioxidants such as N‐acetyl cysteine by increasing the membrane''s permeability leads to lower cytotoxicity. In this study, N ‐acetyl cysteine‐PLGA Nano‐conjugate was synthesized according to stoichiometric rules of molar ratios andafter assessment by FTIR, NMR spectroscopy and Atomic Force Microscopy (AFM) imaging was combined with Magnevist® (gadopentetate dimeglumine) and its effects on the renal cells were evaluated. MTT [3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide] and cellular uptake assays have indicated relatively significant toxicity of magnevist (P  < 0.05) on three cell lines including HEK293, MCF7 and L929 compared to other synthesized ligands that shown no toxicity. Moreover, systemic evaluation has shown no notable changes of blood urea nitrogen (BUN) and creatinine in kidney of mice. In consequence, antioxidant effect was increased as well as the renal toxicity of the contrast agent reduced at the cell level. As a result, PLGA‐NAC nano‐conjugate can be a promising choice for decreasing the magnevist toxicity for treatment and prevention of CIN and will be able to open a new horizon to research on reduction of toxicity of contrast agents by using nanoparticles.Inspec keywords: blood, toxicology, nanofabrication, cellular biophysics, biomedical materials, nanoparticles, chromatography, cancer, biodegradable materials, biomedical MRI, kidney, pH, nanomedicine, patient treatment, diseases, atomic force microscopy, Fourier transform infrared spectraOther keywords: cellular toxicity, gadopentate dimeglumine, contrast agent, magnetic resonance imaging technique, diethylenetriamine pentaacetate, contrast‐induced nephropathy, nephrogenic systemic fibrosis, stoichiometric rules, molar ratios, dimethyl sulphoxide solution, chromatography techniques, nuclear magnetic resonance spectroscopy, atomic force microscopy imaging, Magnevist®, gadopentetate dimeglumine, renal cells, MTT cytotoxicity, human embryonic kidney‐293, L929 cell lines, in vitro conditions, cellular uptake assays, Magnevist uptake, antioxidant effect, renal toxicity, cell level, PLGA nanocarrier, acetylcysteine nanoconjugate, Magnevist toxicity, N‐acetylcysteine–PLGA nano‐conjugate, N‐acetyl cysteine‐poly‐lactic‐co‐glycolic acid nanoconjugate     

Prediction of restrained shrinkage based on restraint factors in patching repair mortar

Shrinkage of repair material, especially in patching repairs, is the major factor inducing cracking in concrete repairs. Induced cracks in repair materials are due to restrained shrinkage. Although in usual practice, the free shrinkage of the repair mortar is measured, in reality, cracking is not due to free shrinkage. It is well known that cracking is due to restrained shrinkage. It is very hard to measure the restrained shrinkage; therefore, to overcome this problem a restraint factor (R) is used to modify the free shrinkage and come up with the restrained shrinkage. The restraint factor is influenced by the surface and boundary condition.In this study, the restraint factor for patching repair with different boundary conditions (with eaves and without eaves) and surface condition (rough and smooth) of the substrate concrete is investigated.The results show that the restraint factor R lies between 0.1 and 0.94; with an increase of restraint, the restraint factor is increased. In situations with a high level of restraint (eaves at the perimeter and a rough surface of substrate), the average R is 0.83. while with a low level of restraint (without eaves at the perimeter and a smooth surface of the substrate), the average R is 0.22.     

Some simple management criteria for confined Saq aquifer in Tabuk region,Saudi Arabia

A simple management model for irregularly located wells is presented by considering interference between adjacent wells at a certain risk level and safe groundwater velocity which depends mainly on the hydraulic conductivity at individual well sites. The final decision variables for a confined aquifer are the maximum allowable discharge, velocity, and drawdown quantities. Regional variations of these quantities are presented in the form of maps which are most useful tools for identifying mismanagement areas and, accordingly, the necessary precautions can be taken. The application of the management model is performed with the actual field data from the Saq formation in the north-western part of the Kingdom of Saudi Arabia.     

The effect of SiO2 additions on barium aluminate cement formation and properties

Barium aluminate cements have been synthesized by barium carbonate, alumina, kaolin and colloidal silica as starting materials. The effects of the source of SiO₂ and of firing temperature on phase formation and physical properties of the fired cements have been studied. Cement samples were characterized using XRD, SEM, EDX. The setting time and heat of hydration of cements were also evaluated. The barium aluminate cements were mixed in castables. Cold crushing strengths evaluated, and values compared to those obtained using calcium aluminate cement (Secar 71). Mixtures of BaCO₃ and Al₂O₃ were targeted to produce BaAl₂O₄; which had fast set time, expansive behavior and lower strength compared to samples with SiO₂ additions. SiO₂ additions, regardless of source, resulted in BaAl₂Si₂O₈ (celsian) formation. The prepared samples had short setting times and higher mechanical properties in comparison with standard calcium aluminate cement.     

Microbial conversion of androst‐1,4‐dien‐3,17‐dione by Mucor racemosus to hydroxysteroid‐1,4‐dien‐3‐one derivatives

BACKGROUND: A large number of bacterial, fungal and microalgal species are able to bio‐transform steroid compounds. Among them, fungi from the Mucor genus have been shown to mediate hydroxylation, oxidation, and desaturation by the double bond formation and epoxidation of various steroid substances. Mucor racemocus has not been studied for its ability to modify androst‐1,4‐dien‐3,17‐dione, a pharmaceutically important steroid precursor. RESULTS: The filamentous fungus M. racemosus was applied for bioconversion of androst‐1,4‐dien‐3,17‐dione (ADD, I ) in a 5‐day fermentation. Microbial metabolites were purified chromatographically and identified on the basis of their spectral data as 17β‐hydroxyandrost‐1,4‐dien‐3‐one ( II ), 14α‐hydroxyandrost‐1,4‐dien‐3,17‐dione ( III ), 15α‐hydroxyandrost‐1,4‐dien‐3,17‐dione ( IV ), 15α,17β‐dihydroxyandrost‐1,4‐dien‐3‐one ( V ), 14α,17β‐dihydroxyandrost‐1,4‐dien‐3‐one ( VI ), and 6β,17β‐dihydroxyandrost‐1,4‐dien‐3‐one ( VII ). CONCLUSION: Observed modifications included hydroxylation at C‐6β, C‐14α, C‐15α positions and 17‐carbonyl reduction. The best fermentation conditions for production of hydroxysteroid‐1,4‐dien‐3‐one derivatives were found to be 25 °C at 150 rpm for 5 days with a substrate concentration of 0.5 g L^?1. Copyright © 2009 Society of Chemical Industry     

High yield gold nanoparticle‐based DNA isolation method for human papillomaviruses genotypes from cervical cancer tissue samples

Gold nanoparticles (AuNPs) are commonly used in biosensors of various kinds. However, its application to extract DNA from cancer tissues has not been extensively studied. The purification of DNA from cancer tissues is an important step in diagnostic and therapeutic development. Almost, all cervical cancer cases are associated with high‐risk human papillomavirus (HR‐HPV) infection. Accurate viral diagnosis has so far relied on the extraction of adequate amounts of DNA from formalin‐fixed, paraffin‐embedded (FFPE) tissue samples. Till now, no specific and sensitive DNA purification method has been introduced for the extraction of HR‐HPV from FFPE tissue. Since the commercially available purification kits are not sensitive and specific enough for HR‐HPV DNA targets, in this study, a DNA purification method was designed based on AuNPs to purify sufficient amounts of HR‐HPV DNA from cervical cancer tissue samples. AuNPs were coated with a series of oligonucleotide probes to hybridize to specific DNA sequences of HR‐HPV genotypes. Results showed that 733 out of 800 copies of type‐specific HPV DNA were recovered with complete specificity, compared to 36 copies with a standard commercial kit (Qiagen FFPE). The high yield of DNA (91.6%) is the main advantage of the AuNPs‐probe purification method.Inspec keywords: cancer, biological tissues, microorganisms, gold, DNA, lab‐on‐a‐chip, gynaecology, purification, biochemistry, molecular biophysics, nanoparticles, nanomedicine, cellular biophysicsOther keywords: specific extraction method, cervical cancer tissue samples, high‐risk human papillomavirus infection, paraffin‐embedded tissue samples, specific DNA purification method, sensitive DNA purification method, FFPE tissue, HR‐HPV DNA targets, specific DNA sequences, HR‐HPV genotypes, type‐specific HPV DNA, AuNP‐probe purification method, gold nanoparticle‐based DNA isolation method, oligonucleotide, human papillomaviruse genotypes, clinical samples, Au     

Protein Kinase C as a Therapeutic Target in Non-Small Cell Lung Cancer

Driver-directed therapeutics have revolutionized cancer treatment, presenting similar or better efficacy compared to traditional chemotherapy and substantially improving quality of life. Despite significant advances, targeted therapy is greatly limited by resistance acquisition, which emerges in nearly all patients receiving treatment. As a result, identifying the molecular modulators of resistance is of great interest. Recent work has implicated protein kinase C (PKC) isozymes as mediators of drug resistance in non-small cell lung cancer (NSCLC). Importantly, previous findings on PKC have implicated this family of enzymes in both tumor-promotive and tumor-suppressive biology in various tissues. Here, we review the biological role of PKC isozymes in NSCLC through extensive analysis of cell-line-based studies to better understand the rationale for PKC inhibition. PKC isoforms α, ε, η, ι, ζ upregulation has been reported in lung cancer, and overexpression correlates with worse prognosis in NSCLC patients. Most importantly, PKC isozymes have been established as mediators of resistance to tyrosine kinase inhibitors in NSCLC. Unfortunately, however, PKC-directed therapeutics have yielded unsatisfactory results, likely due to a lack of specific evaluation for PKC. To achieve satisfactory results in clinical trials, predictive biomarkers of PKC activity must be established and screened for prior to patient enrollment. Furthermore, tandem inhibition of PKC and molecular drivers may be a potential therapeutic strategy to prevent the emergence of resistance in NSCLC.     

Optimization of STM/FIM nanotip aspect ratio based on the Taguchi method

This paper presents optimization of electrochemical etching parameters to achieve the optimum aspect ratio of the scanning tunneling microscopy/field ion microscopy tungsten nanotip by using Taguchi method. The combination of optimum level of process parameters was obtained by using the analysis of signal-to-noise ratio. The level of importance of the process parameters on the nanotip aspect ratio was determined by using analysis of variance. It was found that the optimum level of process parameters are electrolyte concentration of 2 M/lit, wire immersion length of 2.5 mm, cathode tube inner diameter of 40 mm, and voltage of 3.5 V. Within the range of experiments and the process parameters in terms of impact significance were found to be electrolyte concentration, process voltage, wire immersion length, and inner diameter of cathode tube, respectively. By using the optimum level of the process parameters, the nanotip aspect ratio was enhanced by 263% in comparison to the mean value of the experimental results. The nanotip aspect ratio of up to 163:1 was obtained in the present research.     

On robust stability of linear time invariant fractional-order systems with real parametric uncertainties

In this paper, the robust bounded-input bounded-output stability of a large class of linear time invariant fractional order families of systems with real parametric uncertainties is analyzed. The transfer functions contain polynomials in fractional powers of the Laplace variable s, possibly in combination with exponentials of fractional powers of s. Using the concept of the value set and a generalization of the zero exclusion condition theorem, a theorem to check the robust bounded-input bounded-output stability of these families of systems is presented. An upper cutoff frequency for drawing the value sets is provided as well. Finally, two numerical examples are given to illustrate results obtained by the lemma and theorems presented in the paper.